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Ohtsubo S Taiyoji M Kawase T Taniguchi M Saitoh E 《Journal of agricultural and food chemistry》2007,55(5):1762-1766
We examined the biochemical and structural properties of oryzacystatin-II, a phytocystatin in rice (Oryza sativa L. japonica), under heat-stress conditions. The enzyme inhibitory reactivity of oryzacystatin-II was enhanced by heating in a temperature-dependent manner and reached a maximum level by heating at 65 degrees C for 10 min. Size-exclusion chromatography showed that oryzacystatin-II forms a homodimer at ambient temperature and that the enhancement of inhibitory reactivity is due to the conversion of the dimeric to a monomeric form. The monomeric form of oryzacystatin-II reverted to the dimer during storage at 4 degrees C, suggesting that dimerization is an intrinsic property of oryzacystatin-II. The affinity of the monomer for cysteine proteinases was significantly higher than that of the dimer. This is the first paper to describe the noncovalent dimerization for a cystatin under nonstress conditions. 相似文献
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Annual net production of the five Sargassaceae species in Yoro,western Wakasa Bay,Sea of Japan 总被引：1，自引：0，他引：1
Kousuke?YatsuyaEmail author Tomokazu?Nishigaki Akio?Douke Yozo?Wada 《Fisheries Science》2005,71(5):1098-1106
ABSTRACT: Annual net production of the five Sargassaceae species was investigated in Yoro, western Wakasa Bay, Sea of Japan. The Sargassaceae species were collected at a depth of 2.0–2.5 m and subjected to stratified clipping for 13 months. Annual net production was estimated at 2407 g dry wt/m2 for Sargassum patens , 2132 g dry wt/m2 for Sargassum macrocarpum , 1458 g dry wt/m2 for Sargassum siliquastrum , 1197 g dry wt/m2 for Myagropsis myagroides , and 1471 g dry wt/m2 for Sargassum piluliferum . The ratio of annual net production to maximum biomass (P/Bmax ) for these species ranged between 1.3 and 1.7, being higher than in previous studies which used the same method. Winter storms with strong wave exposure and relatively calm condition in Yoro were considered to augment P/Bmax . The total losses estimated from the different units of stratified clipping were compared. If larger and less segregated units were applied, the total loss would decrease. It was suggested that a 50 cm stratum would be applicable to the larger species (3 m or longer) and a stratum of 20 cm or less would be applicable to mid-sized or smaller species (less than 3 m). 相似文献
3.
Tomokazu Hagihara Masashi Ando Kenichi Kawasaki Yasuo Makinodan Yasuyuki Tsukamasa 《Fisheries Science》2006,72(2):393-401
ABSTRACT: The physical properties of 49 commercial kamabokos were measured by the puncture and stress relaxation tests. The principal component analysis was applied to the physical parameters of both tests, and their cumulative contribution ratios were over 90% with the first and second principal components, respectively. The comparison among the kamabokos was carried out using the synthetic physical parameters. The kamabokos produced in same area showed the peculiar distribution. The relative positional relation of kamabokos measured by the stress relaxation test was different from that by the puncture test for many of the kamabokos. Physical property evaluation using a principal component analysis is very effective for intensiveness of many measurement parameters. If much more kamabokos were measured by this method, regional characteristics of kamabokos would be clarified. 相似文献
4.
Masafumi Katayama Tomokazu Fukuda Toshimitsu Hatabu Kiyoaki Narabara Asaki Abe Yasuhiro Kondo 《Animal Science Journal》2014,85(3):277-285
In chickens, although estrogen receptors (ER) are reported to be associated with the immunological processes, detailed information about the differences in ER expression in the tissues related to the development of lymphocytes is not fully known, especially during the developmental stage. To learn more about this immunological relationship, we used semi‐quantitative polymerase chain reaction method to detect the ER expression levels in the thymus tissues of chicks during the developmental stage. Furthermore, ER‐expressing cells were detected by immunohistochemistry. The results of this study show that the expression level of ER increased on embryonic day 16 and decreased on day 20. Furthermore, ER expression was significantly higher in male than in female chickens at day 16. The increased expression on day 16 and decreased level on day 20 were also reproduced in the incidence of immunoreactive cells, although there was a 1‐day delay in the elevated incidence of the cells. This study revealed the changes in ER expression and the incidence of ER‐positive cells in the thymus of chickens during the developmental stage. 相似文献
5.
Recent developments in laser spectroscopy of atomic ions stored in electromagnetic traps are reviewed with emphasis on techniques that appear to hold the greatest promise of attaining extremely high resolution. Among these techniques are laser cooling and the use of single, isolated ions as experimental samples. Doppler shifts and other perturbing influences can be largely eliminated. Atomic resonances with line widths of a few parts in 10(11) have been observed at frequencies ranging from the radio frequency to the ultraviolet. Experimental accuracies of one part in 10(18) appear to be attainable. 相似文献
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Masafumi KATAYAMA Tomokazu FUKUDA Toshihiro OKAMURA Eisaku SUZUKI Katsuo TAMURA Yuuko SHIMIZU Yoshihito SUDA Keiichi SUZUKI 《Animal Science Journal》2011,82(2):274-281
In pig production, dietary additive antibiotics are usually used for growth stimulation and disease prevention, although there is public concern about the increased incidence of resistant antibiotics and food safety. It is possible that such antibiotics might be replaced by naturally derived products such as seaweed and licorice. In this study, we evaluated the effect of dietary addition of seaweed and licorice on enhancing the immune function in swine. The animals of each group (eight animals per group) were sensitized at day 42 and 49, and the immunoglobulin production and the expression of cytokines were detected by the ELISA and real‐time PCR. As the results, saliva IgA production of the seaweed‐treated group increased around five times compared to that of control (day 56). Delayed hypersensitivity reaction and IgG production of the seaweed‐treated group increased around 1.8–2.0 times. In addition, enhanced saliva IgA production was detected at day 50 (around two times) and day 51 (around five times) by the licorice treatment, and lower expression level of tumor necrosis factor‐α messenger RNA at day 51 (around 1/25) was observed in the licorice treatment. We conclude that the replacement of antibiotics by naturally derived dietary additives might be feasible for immune system enhancement. 相似文献
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从植物营养的观点出发,转化土壤中不溶性有机磷和无机磷化合物为可溶性磷酸盐的过程具有极重大的意义,结果可以大大地提高土壤的生产力。最近,P.A.曼吉娜(Р.А.Менкина)指出:Bacillus megatherium var .phos-phaticum 与 Serratia var.phosphaticum 能矿化卵磷脂或核酸一类的有机磷化合 相似文献
9.
Dietary intake of iodine‐enriched eggs decreases the incidence of mouse mammary tumors caused by the activated ErbB2 oncogene 
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下载免费PDF全文 Kazunori Oyama Takahiro Shimoda Makoto Miyagawa Mizuki Sone Jiro Yokoyama Katsuhiko Nishimori Tomokazu Fukuda 《Animal Science Journal》2018,89(8):1169-1177
Human epigenetic studies suggest that consumption of seaweed prevents mammary cancer, which possibly is explained by iodine daily intake. In this study, we evaluated the efficacy of dietary intake of iodine‐enriched eggs on mammary tumor incidence caused by the expression of activated type ErbB2. Female transgenic mice were divided into three groups, and fed a basic diet, a diet supplemented with ordinary eggs, or with iodine‐enriched eggs. The number of mammary tumors greater than 5 mm in diameter was recorded in mice at 6 months of age. We report that the average number of mammary tumors per mouse was significantly lower in the iodine‐enriched egg‐added diet group than in either the basic diet or ordinary egg diet groups. These results indicate that iodine intake through livestock‐derived products can reduce the incidence of mammary cancers caused by the expression of activated type ErbB2. 相似文献
10.
TB Mitchell JJ Bollinger DHE Dubin X Huang WM Itano RH Baughman 《Science (New York, N.Y.)》1998,282(5392):1290-1293
Laser-cooled 9Be+ ions confined in two-dimensionally extended lattice planes were directly observed, and the images were used to characterize the structural phases of the ions. Five different stable crystalline phases were observed, and the energetically favored structure could be sensitively tuned by changing the areal density of the confined ions. The experimental results are in good agreement with theoretical predictions for the planar (infinite in two dimensions) one-component plasma. Qualitatively similar structural phase transitions occur, or are predicted to occur, in other experimentally realizable planar systems. 相似文献