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Yasmin Vasques Berchembrock Ulisses José de Figueiredo José Airton Rodrigues Nunes Cacilda Borges do Valle Sanzio Carvalho Lima Barrios 《Grass and Forage Science》2020,75(2):145-152
Hybridization in the Urochloa humidicola breeding programme allowed to explore the genetic variability of the apomictic sources and, thus, to obtain new sexual and apomictic parents with compatible ploidy and/or superior progenies. However, given the high variability arising from crosses, there is a need to adopt efficient selection strategies among and within progenies. The aim of this study was to compare the selection methods among U. humidicola progenies with (t-BLUP) and without (BLUP) the weighting based on prediction error and subsequent selection within progenies by individual simulated (BLUPIS) or optimal individual BLUP. We evaluated seventy-one U. humidicola full-sib progenies from biparental crosses between nine sexual and ten apomictic parents. The experiment was set up in an incomplete block design with 64 blocks of 15 plots each, consisting of individual plants. Seven consecutive cuts were made and the traits total dry-matter, leaf dry-matter and regrowth scores were measured. Genetic variance was expressive for all traits. The selection procedure among progenies of U. humidicula based on t-BLUP proved to be more advantageous regarding the use of BLUP not weighted by the prediction error, resulting in higher selection gains, taking as reference the optimal procedure of the individual BLUP. Furthermore, the use of BLUPIS allowed an optimized selection of hybrids, allowing the breeder to explore with variable selection intensity the genetic variability within the selected full-sib progenies based on relative genetic merit. 相似文献
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A literature survey was conducted to determine the amphibian diversity and distribution in Brazilian Amazonia. Patterns of endemisms and similarity of fauna between localities were also addressed. Twenty-eight inventories were found for the region, the majority localized in areas with easier access by road or river. A minimum of 163 amphibian species was recorded for Brazilian Amazonia. Although many species are endemic to the Amazonian lowlands as an entity, the patterns of species uniqueness among sites suggested low endemic distribution within the lowlands of the Amazon Basin. The mean similarity between localities varied from low to intermediate (mean=0.40), indicating that the Brazilian Amazonia is characterized by distinctive assemblages of amphibians throughout its extension. Localities further apart had lower similarity. No threatened species were recorded. These results contribute to determine priority areas for new inventories and establishment of conservation units. We suggest that areas next to the Amazon deforestation frontier should be prioritized for new studies due to the high rate of alteration and potential loss of species. Additionally, studies on amphibian population dynamics are few in Brazilian Amazonia and more of them should be emphasized to help to draw a better picture of the status of amphibians in this region. 相似文献
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Barbosa LC Pereira UA Teixeira RR Maltha CR Fernandes SA Forlani G 《Journal of agricultural and food chemistry》2008,56(20):9434-9440
The [4 + 3] cycloaddition of the proper furans with the oxyallyl cation, generated in situ from 2,4-dibromopentan-3-one, produced a series of 8-oxabicyclo [3.2.1]oct-6-en-3-ones. Exposure of the oxabicycles to ozone afforded the corresponding 8,9,10,11-tetraoxatricyclo[5.2.1.1 (2,6)]undecan-4-ones in variable yields (7-100%). The phytotoxic properties of these ozonides (or 1,2,4-trioxolanes) and their oxabicycle precursors were evaluated as the ability to interfere with the growth of Sorghum bicolor and Cucumis sativus seedlings. Among oxabicycles, the highest inhibitory activity was shown by compounds possessing a alpha,beta-unsaturated carbonyl moiety. A differential sensitivity of the two crops was evident with ozonides. The most active compounds were also tested against the weed species Ipomoea grandifolia and Brachiaria decumbens. To the best of our knowledge, this is the first article describing ozonides as potential herbicides. 相似文献
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Derossi R Medeiros U de Almeida RG Righetto FR Frazílio FO 《Veterinary journal (London, England : 1997)》2008,178(2):294-297
The aim of the study was to evaluate and compare the effects of caudal epidural administration of meperidine (MP), lidocaine (LD), and a combination of the two (MPLD) in six mature saddle horses. Horses were randomly assigned to receive three treatments (MP 0.3 mg/kg; LD 0.2 mg/kg; and MPLD: MP 0.3 mg/kg and LD 0.2 mg/kg), with at least 1 week between treatments. Drugs were injected into the epidural space between the first and second coccygeal areas in conscious standing horses. Analgesia, ataxia, sedation, cardiovascular and respiratory effects, and rectal temperature were recorded at different intervals before (baseline) and after administration. Epidural administration of MPLD resulted in a longer duration of analgesia of the tail, perineum, and upper hind limb regions than did administration of MP or LD alone. 相似文献
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Weishou Shen Juan M. Cevallos-Cevallos Ulisses Nunes da Rocha Hector A. Arevalo Philip A. Stansly Pamela D. Roberts Ariena H. C. van Bruggen 《European journal of plant pathology / European Foundation for Plant Pathology》2013,137(4):727-742
Intensive insecticide and nutrient management have been attempted worldwide to reduce citrus huanglongbing (HLB) symptom development and yield loss. However, effects of insecticide and nutrient applications on HLB have been poorly understood. Leaf nutrients, jasmonic and salicylic acid contents, cycle threshold (Ct) values of Ca. Liberibacter asiaticus (Las), and community structure of endophytic α-proteobacteria were evaluated after insecticide treatment, ‘nutrition’ treatment (including systemic resistance inducing agents), or both in comparison with a control in a two-factor field experiment in 2008–2012. Leaf N, Mn, Zn and B significantly increased whilst Cu decreased after nutrient applications. Salicylic acid significantly increased in old leaves treated with insecticides, nutrients or both, and in young leaves treated with nutrients only. The jasmonic acid concentration was highest after the nutrition treatment in both old and young leaves. Ct values of Las and leaf area and weight significantly increased after long-term nutrient applications in 2011 and/or 2012. Redundancy analysis of the endophytic α-proteobacteria community structure indicated that the communities were mainly separated according to nutrient applications, which were positively associated with Ct values of Las and Ca, Mn, Zn, B, Mg, and Fe contents in leaf samples collected in 2012. Thus, effects of insecticides on HLB were significant in the early 2-year period whilst nutrients had significant effects on Las content and leaf size and weight after at least 3 years of application. 相似文献
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Ulisses Jos de Figueiredo Sanzio Carvalho Lima Barrios Cacilda Borges do Valle Jos Airton Rodrigues Nunes 《Grass and Forage Science》2019,74(4):678-686
The diallel cross constitutes an informative genetic design for choosing genitors and crosses in breeding programmes since it provides estimates of general (GCA) and specific (SCA) combining abilities. Because the breeding programme for Urochloa humidicola ([Rendle] Morrone & Zuloaga; syn. Brachiaria humidicola [Rendle] Schweick) at Embrapa Beef Cattle is recent, these estimates are still unknown. Thus, the aim of this study was to obtain the estimates of GCA and SCA from the partial diallel cross design between sexual and apomictic parents. The crosses involved nine sexual and ten apomictic parents. Seventy‐one full‐sib progenies were obtained and evaluated in an incomplete block design, using all parents and the cv. BRS Tupi as a check. The agronomic and forage quality traits were evaluated using seven harvests. Statistical analysis was performed using the mixed model approach. Significant variations were associated with GCA among sexual parents for biomass yield and forage quality traits. However, this cannot be observed for apomictic parents for GCA. The SCA effect was not significant for the assessed traits. These results showed the predominance of additive effects. Sexual parents SEX3 and SEX4 presented the highest potential for contributing favourable alleles in hybrid combinations. The absence of GCA among apomictic parents and SCA suggests the need to increase genetic variability for the generation of breeding populations of U. humidicola. 相似文献
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da Rocha Ulisses Nunes Andreote Fernando Dini de Azevedo João Lúcio van Elsas Jan Dirk van Overbeek Leo S. 《Journal of Soils and Sediments》2010,10(2):326-339
Purpose
The role of dominant bacterial groups in the plant rhizosphere, e.g., those belonging to the phyla Acidobacteria and Verrucomicrobia, has, so far, not been elucidated, and this is mainly due to the lack of culturable representatives. This study aimed to isolate hitherto-uncultured bacteria from the potato rhizosphere by a combination of cultivation approaches. 相似文献8.
Pandolfi Victor César Freitas Yamachita Andrei Lincoln de Souza Felipe Pinheiro de Godoy Sara Mataroli de Lima Ed Christian Suzuki Feliciano Daniele Cassiano de Pádua Pereira Ulisses Povh Jayme Aparecido Ayres Denise Rocha Bignardi Annaiza Braga Penafort Jefferson Murici de Fátima Ruas Claudete Lopera-Barrero Nelson Mauricio 《Aquaculture International》2021,29(6):2435-2449
The objective of the present work was to develop species-specific microsatellite markers for P. scalare and to analyze the diversity and genetic structure of a wild population, from the Amazon River, and three commercial stocks (common, marble, and clown morphological variants), from farmers in Vieras-Minas Gerais. Through microsatellite-enriched genetic libraries, 11 microsatellite markers with adequate amplification patterns were characterized. Population genetic analysis identified eight polymorphic loci that generated 66 alleles ranging from two alleles (PSCA1B3) to nine (PSCA2H1). The polymorphic information content ranged from 0.031 to 0.827. High genetic differentiation was observed between the wild population and the stocks, and moderate differentiation between the three stocks. Deviation in the Hardy-Weinberg equilibrium was observed in one locus in the wild population, in five loci in the common morphological variant, in two in the marble, and in two in the clown morphological variant. Bayesian analysis of genetic structure revealed the existence of two clusters, one represented by the natural population and the other by the stocks. The developed microsatellite markers serve as a tool for the analysis of diversity and genetic structure and conservation studies of P. scalare.
相似文献9.
DeRossi R Almeida RG Medeiros U Righetto FR Frazílio FO 《Veterinary journal (London, England : 1997)》2007,173(3):658-663
To determine the efficacy and safety of subarachnoid butorphanol combined with lidocaine, six calves were studied. Each calf underwent two treatments, at least one week apart, via subarachnoid injection: (1) butorphanol (0.03 mg/kg) plus 2% lidocaine (4 mg/kg) and (2) 2% lidocaine (4 mg/kg) alone. Subarachnoid injections were performed at the lumbosacral space. Analgesia, motor block, sedation, heart rate, arterial blood pressure, respiratory rate, arterial oxygen saturation as measured by pulse oximetry, and rectal temperature were compared before and after subarachnoid administration of drugs. Subarachnoid administration of the butorphanol-lidocaine combination induced bilateral prolonged analgesia extending from the coccygeal to the T11-T13 dermatomes in the calves, with minimal sedation and severe ataxia. Cardiovascular effects were significant in both treatments: heart rate was increased, and there was a minimal decrease in arterial pressure. It was concluded that adding a small dose of butorphanol to subarachnoid lidocaine in calves is effective and safe. 相似文献
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Rafael Alves Vianna Roberta Torres Chideroli Arthur Roberto da Costa Oswaldo Pinto Ribeiro Filho Leandro Licursi de Oliveira Juarez Lopes Donzele Eduardo Arruda Teixeira Lanna Daniela Dib Gonalves Ulisses de Padua Pereira 《Aquaculture Research》2020,51(3):1276-1283
Streptococcosis causes serious economic losses to fish farms every year. A four‐week feeding trial was carried out to evaluate arginine (ARG) supplementation in the diet of Nile tilapia (Oreochromis niloticus) fingerlings challenged by Streptococcus agalactiae. Fish were fed with experimental diets containing five levels of supplementation with 1.39%, 1.76%, 1.97%, 2.18% or 2.39% analysed level of ARG. Each diet was randomly distributed in 30 tanks containing 20 fish/tank. After 30 days, no differences were observed in performance parameters, weight gain, daily weight gain, specific growth rate, feed intake or food conversion. After the performance assay, fingerlings were infected with 1.95 × 108 CFU/fish of Streptococcus agalactiae. Mortality was verified daily, and the respiratory burst of leucocytes and nitric oxide production were measured at 0 hr, 24 hr, 7 days and 15 days after infection. The survival rate of the fish is compared using the Kaplan–Meier method, with values of 4.29%, 14.8%, 10.8%, 17.2% and 33.3% for the 1.39, 1.76, 1.97, 2.18 and 2.39 treatments respectively. A reduction in the mortality rate and a boost in the immune responses was observed in all the supplementations of ARG in the diet; however, the best survival rates were obtained in the treatments with 2.39% ARG as well as the most efficient immune responses. 相似文献
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