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1.
Wei-Na Wang An-Li Wang Yue Wang Junxia Wang Ru-Yong Sun 《Journal of the World Aquaculture Society》2005,36(1):1-7
This study examined the effects of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponese . The response of the cellular defense was determined by monitoring changes in reactive oxygen intermediates in hemocytes, superoxide dismutase activity, catalase activity, glutathione peroxidase activity, glutathione reductase and glutathione transferase activity in muscle. The results showed that prawns fed the basal diet had a significant increase in reactive oxygen intermediates in response to exposure to ammonia-N. When dietary vitamin C was added to the feed, there was a significant decrease in reactive oxygen intermediates in response to exposure to ammonia-N. Total superoxide dismutase and glutathione peroxidase activity of prawns fed the basal diet decreased as ammonia-N increased. This decrease in activity was significantly less pronounced in prawns that were fed the dietary vitamin C supplement. In prawns that were fed the dietary vitamin C supplement there was no significant variation in catalase reductase or gluxathione reductase activity in response to variations in ammonia-N concentration. The glutathione transference activity increased at a low concentration of ammonia-N in prawns that were fed the dietary vitamin C supplement, but there was no significant variation in the prawns fed the basal diet. Overall the results indicate that dietary vitamin C supplements enhance the capacity of M. nipponese to respond to elevations in ammonia-N. This response involved several biochemical reactions known to be involved in cellular defense. 相似文献
2.
GUAN Cang-hai ZHAO Yu-qiao Guo Liang CHEN Yu-Zhu WANG Wei-Na JIANG Xing-ming 《园艺学报》2000,36(10):1908-1912
MNX1 antisense RNA 1 （MNX1-AS1） is a newly discovered long non-coding RNA （lncRNA）， which is highly dysregulated in various carcinomas and its expression level is closely related to the overall survival and prognosis of patients. MNX1-AS1 regulates the occurrence and development of carcinomas by endogenous competitive adsorption of miRNA， regulating cell cycle， inducing epithelial mesenchymal transformation and activating multiple signaling pathways. The in-depth study of the carcinogenesis of MNX1-AS1 is useful for the early diagnosis， targeted therapy and prognostic assessment of relevant carcinomas. This article reviews the roles of MNX1-AS1 in malignant tumor. 相似文献
3.
An-Li Wang Wei-Na Wang Yue Wang Li-Xin Shang Yuan Liu Ru-Yong Sun 《Aquaculture (Amsterdam, Netherlands)》2003,220(1-4):833-841
The 96-h LC50 of ammonia-N and the effects of dietary vitamin C on oxygen consumption, ammonia-N excretion and Na+/K+ ATPase activity of Macrobrachium nipponense exposed to ambient ammonia were investigated. The results showed that the 96-h LC50 of ammonia-N was 36.6 mg l−1 for the freshwater prawn, M. nipponense, at pH 8.0. When prawns were exposed to high ambient ammonia-N concentrations, the oxygen consumption rate increased and ammonia excretion decreased. Dietary vitamin C supplementation led to higher oxygen consumption and lower ammonia excretion. Na+/K+ ATPase activity increased with increased ambient ammonia-N exposure in the range of 0–18.3 mg l−1, and then was reduced at ambient ammonia-N 36.6 mg l−1. Na+/K+ ATPase activities of prawns fed a vitamin C-supplemented diet were significantly lower than those of prawns fed a diet which was not supplemented with vitamin C. 相似文献
4.
Kang-Le Lu Li-Na Wang Ding-Dong Zhang Wen-Bin Liu Wei-Na Xu 《Fish physiology and biochemistry》2017,43(1):65-76
High-fat diets may have favorable effects on growth and cost, but high-fat diets often induce excessive fat deposition, resulting in liver damage. This study aimed to identify the hepatoprotective of a Chinese herb (berberine) for blunt snout bream (Megalobrama amblycephala). Fish were fed with a normal diet (LFD, 5 % fat), high-fat diet (HFD, 15 % fat) or berberine-supplemented diets (BSD, 15 % fat with berberine 50 or 100 mg/kg level) for 8 weeks. After the feeding, histology, oxidative status and mitochondrial function of liver were assessed. The results showed that HFD caused fat accumulation, oxidative stress and apoptosis in hepatocytes of fish. Hepatocytes in HFD group appeared to be hypertrophied, with larger liver cells diameter than these of LFD group. Berberine-supplemented diets could attenuate oxidative stress and hepatocytes apoptosis. HFD induced the decreasing mitochondrial complexes activities and bulk density and surface area density. Berberine improved function of mitochondrial respiratory chain via increasing the complex activities. Moreover, the histological results showed that berberine has the potential to repair mitochondrial ultrastructural damage and elevate the density in cells. In conclusion, our study demonstrated that berberine has attenuated liver damage induced by the high fat mainly via the protection for mitochondria. 相似文献
5.
Catharanthus roseus receptor-like kinase1-like kinases (CrRLK1Ls)是植物所特有的一类蛋白类受体激酶,在维持细胞壁完整性、细胞间通讯、生物和非生物应激反应中发挥重要作用。本研究鉴定了马铃薯CrRLK1Ls (StCrRLK1Ls)基因家族的成员数量,并对其理化特性、染色体位置、进化特征、亚细胞定位和对晚疫病菌侵染时的表达模式进行全面分析。结果显示,共获得17个StCrRLK1Ls基因,其氨基酸序列大小为753～997 aa,分子量和等电点分别为83.34～108.69kD和5.30～7.56,主要位于质膜。进化分析将马铃薯、拟南芥、水稻、苹果和番茄的CrRLK1Ls家族成员分为7个亚组,马铃薯CrRLK1Ls家族成员分布于亚组Ⅲ、Ⅳ、Ⅴ、Ⅵ和Ⅶ。StCrRLK1Ls不均匀的分布于8条染色体上,存在3个串联重复基因簇,包含6个基因。此外,StCrRLK1Ls启动子区域存在响应植物激素、防卫和逆境等多种顺式调控元件。‘大西洋’和‘陇薯7号’接种晚疫病菌(Phytophthora infestans,Pi)后,大量StCrRLK1... 相似文献
6.
草金鱼总色素光谱吸收峰在400～600nm,系类胡萝卜素色素。在不同酸碱度、维生素和有机物作用下,该观赏鱼总色素稳定性各不相同。环境pH值过高或过低对该色素具有强烈的破坏作用,中性pH值溶液利于该色素的有效保存;Vc和VE对该色素具有保护作用,但是当Vc剂量不适时,该色素遭到严重破坏,VK对该色素具较强的破坏作用;乳酸对该色素具有增色作用,苯甲酸、草酸、柠檬酸,丙二酸和反丁烯二酸对该色素具有保护作用,乌头酸、水杨酸、顺丁烯二酸、亚油酸、萘乙酸、溴丙酸和苹果酸对该色素具有破坏作用,其中以苹果酸的破坏作用最强。淀粉对该色素具有保护作用,葡萄糖和蔗糖则具有破坏作用,其中以蔗糖的破坏作用最强。研究结果提示：在提取、保存该色素以及配制增色饵料时,要特别注意避免不良因素的影响,确保试验结论的正确和增色效果的良好。 相似文献
7.
富含半胱氨酸的类受体激酶(cysteine-rich receptor-like kinase,CRK)在植物生长发育和环境适应过程中发挥重要的作用。本研究鉴定了马铃薯CRK(StCRK)家族成员,并对其理化性状、进化特征、亚细胞定位、染色体位置和表达模式进行分析。鉴定获得8个StCRKs,其氨基酸序列大小为459~686 aa,分子量介于50.75~77.50 kD,等电点介于5.84~8.75,主要位于质膜。进化分析将来自马铃薯、拟南芥、香蕉、苹果、水稻、番茄和棉花的CRKs分为9个亚组,2号、3号和5号染色体上的StCRKs分布于亚组I(6个成员)和VI(2个成员);存在2个串联重复基因簇,包含4个成员。StCRKs启动子区域存在多种顺式调控元件,主要响应激素、低温、防卫和逆境等信号。接种晚疫病菌(Phytophthora infestans,Pi)和干腐病菌(Fusarium sulphureum,Fs)后,分别发现8个和6个StCRKs为差异表达。其中,StCRK4和StCRK8响应Pi和Fs信号,在接种以上2种病原菌后,表达量上调8倍以上,推测其响应多个真菌信号,可能在马铃薯对真菌病害的广谱抗性中起重要作用,可作为进一步抗病研究和功能分析的候选基因。 相似文献
8.
Maintaining the balance of intestinal microbiota is vital for the growth and health of crustaceans. The current study explored the influences of the surface layer proteins (SLPs) of Pediococcus pentosaceus on the growth performance, disease resistance, and gut microbial community of the freshwater prawn Macrobrachium rosenbergii. After 28 days of feeding, obvious enhancements in the growth performance were found in the PF (a commercial basic diet + intact P. pentosaceus) group compared to the CF (a commercial basic diet, control) and RF (a commercial basic diet + LiCl-treated P. pentosaceus) groups. Meanwhile, the relative percent survival (RPS) of the PF group was found to be 82.1%, higher than the RF group (RPS = 63.2%) in the challenge test. There were noteworthy differences in the relative richness of Aeromonas among different treatments, and the PF group was the lowest. Whereas the relative abundance of Lactococcus between the CF and RF group did not markedly differences, which was obviously lower than the PF group. The prediction of bacterial phenotype indicated that the pathogenic potential of intestinal microbiota was decreased by increasing dietary SLPs-carrying P. pentosaceus. The results suggested that SLPs-carrying P. pentosaceus can improve growth, modulate intestinal microbiota, and increase the resistance of prawns against Aeromonas hydrophila infection.
相似文献9.
我国湿地保护的立法思考 总被引：4，自引：0，他引：4
湿地被称为“地球之肾”,具有重要的生态价值、经济价值和社会价值。我国湿地面积广大,但近些年来由于各种原因,湿地面积却在急剧减少。因此,迫切需要构建湿地保护的法律制度,为湿地保护提供法律保障。笔者在对我国湿地开发与利用现状及其管理体制、相关立法存在的问题进行分析的基础上,对我国湿地保护立法提出一些立法构想。 相似文献
10.
本试验以都儿菜的嫩茎为材料,对愈伤组织的诱导、不定芽的分化,不定芽的分化继代培养、试管苗的生根、试管苗移栽所需要的条件进行了研究,建立起都儿菜嫩茎的愈伤组织培养及快速繁殖技术体系.结果表明:MS+BA0.5mg.L-1+2,4-D1.4mg.L-1为诱导都儿菜嫩茎愈伤组织的理想培养基;MS+BA0.5mg.L-1是都儿菜愈伤组织和不定芽分化的理想培养基;1/2MS+IAA0.5mg.L-1是都儿菜不定苗生根的理想培养基;与实生苗相比,定植于田间的试管苗生长旺盛,肉质茎单位面积产量提高14%,单株增产24%. 相似文献