Correction to: Predation on glass eels of Japanese eel <Emphasis Type="Italic">Anguilla japonica</Emphasis> in the Tone River Estuary,Japan     

Yoichi Miyake  Aigo Takeshige  Hikaru Itakura  Hajime Itoh  Hiroaki Onda  Akira Yamaguchi  Akihito Yoneta  Kohma Arai  Yulina V. Hane  Shingo Kimura 《Fisheries Science》2018,84(6):1015-1016

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Alessandro Bonanno and Lawrence Busch (eds): Handbook of the international political economy of agriculture and food     

Marie Louise Ryan 《Agriculture and Human Values》2018,35(3):731-732

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三种不同方法显示仔猪小肠肌间神经元的比较     

张晗  张原  赵琪  王瑞玲  滕可导 《猪业科学》2003,20(8):33-35

本研究采用小肠铺片NADPH-d组化法、NADH-d组化法和抗NF免疫组化法分别对0日龄、5日龄、28日龄仔猪的肌间神经元进行了比较研究。结果表明:NADPH-d组化法可以选择性的标记合成NO的神经元亚群,大部分阳性肌间神经元符合DogielI型神经元的形态特征;NADH-d组化法可以非选择性的标记大部分神经元,且采用该法观察到的肌间神经元的密度明显高于NADPH-d组化法,但该法不能清晰的显示肌间神经丛的细微结构,不适合用于神经元类型的观察;抗NF免疫组化法可以清晰的显示肌间神经元的突起,28日龄仔猪体积较大的阳性神经元主要为DogielI型和DogielII型神经元。  相似文献   

水飞蓟对动物机体免疫功能的影响研究   总被引：2，自引：0，他引：2  

何维明  薛振芳 《畜牧兽医学报》1995,26(2):147-153

将小鼠分为四组，分别饲喂１０％水飞蓟、２０％水飞蓟、环磷酰胺（０．０１ｇ／ｋｇ）和空白对照（常规饲料），研究对小鼠免疫功能的影响。实验结果证明，水飞蓟各剂量组能增高小鼠外周血白细胞数目（Ｐ＜０．０１、０．００１）；提高小鼠腹腔巨噬细胞的吞噬率（Ｐ＜０．００１，０．００１）及吞噬指数（Ｐ＜０．０５、０．００１）并随剂量加大有增强的趋势；能促进小鼠淋巴细胞转化，提高淋巴细胞转化百分率（Ｐ＜０．０１、０  相似文献   

几种造林措施对墨西哥柏、银荆保存率及生长的影响     

甘家生  杨卫明  陆斌  张瑾扬  高义  杜明珠  韦淑成  华秀  杨兴仁  张铨 《西部林业科学》1996,(1)

在呈贡劣质山地对墨西哥柏、银荆树植树造林进行了不同整地方式、植塘规格、种植密度、抚育方式的试验。结果：撩壕整地和水平台地的保存率及幼树生长量优于块状整地，且在阴坡深草地段尤为显著。３种植塘规格（６０ｃｍ×６０ｃｍ×６０ｃｍ，５０ｃｍ×５０ｃｍ×５０ｃｍ，４０ｃｍ×４０ｃｍ×４０ｃｍ）对墨西哥柏的保存率和高、径生长差异不显著，对银荆差异极显著，大塘优于小塘。３种种植密度（１．５ｍ×１．５ｍ，１．５ｍ×２ｍ，２ｍ×２ｍ），对幼林期的高、径生长及冠幅大小无显著影响，在３０°以上的陡坡，台地抚育优于菱形、扇形、块状抚育，与对照比较，４种抚育方式与之差异极显著。  相似文献   

试论林业基层单位科技情报的计算机辅助管理     

滕华卿 《林业勘察设计》2004,(2):42-44

通过对林业基层单位科技情报管理现状的分析，提出实施林业科技情报计算机辅助管理的必要性与可行性，进而论述了建立林业科技报情报计算机管理系统的具体方法、步骤、措施及前景展望。  相似文献   

‘Plantibodies’: a flexible approach to design resistance against pathogens     

A. Schots  J. De Boer  A. Schouten  J. Roosien  J. F. Zil Verentant  H. Pomp  L. Bouwman-Smits  H. Overmars  F. J. Gommers  B. Visser  W. J. Stiekema  J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191

Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (V_L) and heavy chain (V_H) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location.  相似文献   

蝴蝶花的组织培养和根状茎的诱导     

赵宏波  陈发棣  房伟民  张聂  郭维明 《园艺学报》2005,32(3):556-556

蝴蝶花(IrisjaponicaThunb.)是鸢尾科鸢尾属的多年生常绿宿根草本植物,地下有横走的根状茎;耐荫,是良好的耐荫地被植物。试验于2003年10月至2004年5月进行。选择健康的蝴蝶花植株,取地下的根状茎,选带节的茎段作为外植体,用洗洁精溶液浸泡4～5min,采用75%乙醇60s结合0.1%HgCl212min进行灭菌。根状茎在培养基MS 6BA1.0mg·L-1 KT2.0mg·L-1 NAA0.1mg·L-1,25℃下黑暗培养,腋芽出芽率达65.4%。黑暗比光照更有利于腋芽的萌发,可能是由于蝴蝶花是阴性植物;在自然条件下其根状茎的生长和顶芽的萌发处于暗环境,离体条件下黑暗培养更符合其…  相似文献   

Characteristics for Practical Use of Attenuated Isolate UA-Fukushima of <Emphasis Type="Italic">Tomato mosaic virus</Emphasis>     

Tsutomu MATSUMOTO  Yuichiro NARA  Hiromitsu FURUYA  Harumi TAKAHASHI  Kiichi TAIRAKO  Hideki YAMAMOTO 《Journal of General Plant Pathology》2002,68(4):382-384

L₁₁A-Fukushima (L₁₁A-F) derived from attenuated isolate LuA of Tomato mosaic virus (ToMV) has the highest ability to cross protect against virulent ToMV among LuA and its derivatives and is stably inherited. Growth, yield, fruit quality and symptom attenuation of inoculated tomato plants did not differ significantly between L₁₁A-F and L₁₁A. The infectivity of progeny viruses in tomato infected with LuA-F was less than 4% of that with virulent ToMV. From these results, L₁₁A-F appears to possess the properties necessary for practical use. To manage L₁₁A-F strictly, a PCR-based assay to detect trace contamination of virulent ToMV in L₁₁A-F preparations was established. Received 10 June 2002/ Accepted in revised form 30 October 2002  相似文献   

Effects of Latent Infection of Stock Plants and Abundance of Thrips on the Occurrence of <Emphasis Type="Italic">Tomato spotted wilt virus </Emphasis>in Chrysanthemum Fields     

Shohei MATSUURA  Shigeru HOSHINO  Hideaki HAYASHI  Tetsuyuki KOHGUCHI  Kyoji HAGIWARA  Toshihiro OMURA 《Journal of General Plant Pathology》2002,68(1):99-102

DAS-ELISA proved to be reliable enough to detect a latent infection by Tomato spotted wilt virus (TSWV) in asymptomatic stock plants of chrysanthemum. A high density of Frankliniella occidentalis, the predominant vector, in the presence of latently infected stock plants resulted in a high incidence of disease in the chrysanthemum production field. The incidence of disease was low when the vector thrips were not abundant in spite of the presence of latently infected stock plants. These results suggest that an infestation of the vector thrips causes severe secondary spread of TSWV originating from latently infected stock plants in chrysanthemum production fields. Received 27 July 2001/ Accepted in revised form 27 November 2001  相似文献