Comparison of selection methods among and within full-sibling progenies in Urochloa humidicola     

Yasmin Vasques Berchembrock  Ulisses José de Figueiredo  José Airton Rodrigues Nunes  Cacilda Borges do Valle  Sanzio Carvalho Lima Barrios 《Grass and Forage Science》2020,75(2):145-152

Hybridization in the Urochloa humidicola breeding programme allowed to explore the genetic variability of the apomictic sources and, thus, to obtain new sexual and apomictic parents with compatible ploidy and/or superior progenies. However, given the high variability arising from crosses, there is a need to adopt efficient selection strategies among and within progenies. The aim of this study was to compare the selection methods among U. humidicola progenies with (t-BLUP) and without (BLUP) the weighting based on prediction error and subsequent selection within progenies by individual simulated (BLUP_IS) or optimal individual BLUP. We evaluated seventy-one U. humidicola full-sib progenies from biparental crosses between nine sexual and ten apomictic parents. The experiment was set up in an incomplete block design with 64 blocks of 15 plots each, consisting of individual plants. Seven consecutive cuts were made and the traits total dry-matter, leaf dry-matter and regrowth scores were measured. Genetic variance was expressive for all traits. The selection procedure among progenies of U. humidicula based on t-BLUP proved to be more advantageous regarding the use of BLUP not weighted by the prediction error, resulting in higher selection gains, taking as reference the optimal procedure of the individual BLUP. Furthermore, the use of BLUP_IS allowed an optimized selection of hybrids, allowing the breeder to explore with variable selection intensity the genetic variability within the selected full-sib progenies based on relative genetic merit.  相似文献   

Effect of supplementation of beef cattle with different protein levels and degradation rates during transition from the dry to rainy season     

Rodolfo Maciel Fernandes  Chafic Mustafé de Almeida  Bruna Caldas Carvalho  João Alexandrino Alves Neto  Verônica Aparecida Costa Mota  Flávio Dutra de Resende  Gustavo Rezende Siqueira 《Tropical animal health and production》2016,48(1):95-101

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Secreted phosphatase activities in trypanosomatid parasites of plants modulated by platelet-activating factor     

Dutra PM  Dias FA  Santos MA  Rodrigues CO  Romeiro A  Attias M  De Souza W  Lopes AH  Meyer-Fernandes JR 《Phytopathology》2001,91(4):408-414

ABSTRACT The secreted phosphatase activities of two trypanosomatid parasites were characterized and compared with supernatants of living cells. The plant parasite Phytomonas fran?ai and the phytophagous hemipteran parasite Herpetomonas sp. hydrolyzed p-nitrophenylphosphate at a rate of 15.54 and 6.51 nmol Pi/mg of protein per min, respectively. Sodium orthovanadate (N(a)VO(3)) and sodium fluoride (NaF) decreased the phosphatase activities. The phosphatase activity of P. fran?ai was drastically diminished (73% inhibition) in the presence of sodium tartrate, whereas the phosphatase activity of Herpetomonas sp. was inhibited by 23%. Cytochemical analysis showed the localization of these enzymes on the external surface and in the flagellar pocket of the two trypanosomatids. Sodium tartrate inhibited this reaction, confirming the biochemical data. Platelet-activating factor modulated the phosphatase activities, inhibiting P. fran?ai activity and stimulating Herpetomonas sp. phosphatase activity.  相似文献   

Poisoning of horses by bamboo, Bambusa vulgaris     

Jos Diomedes Barbosa  Carlos Magno C. de Oliveira  Marcos Dutra Duarte  Gabriela Riet-Correa  Paulo Vargas Peixoto  Carlos Hubinger Tokarnia 《Journal of Equine Veterinary Science》2006,26(9):393-398

The clinical and pathological aspects of a neurological disease observed in 16 horses in Pará, Amazonia, Brazil, are presented. The symptoms were mainly motor incoordination, paresis of the tongue, somnolence, difficulties in apprehension, chewing and swallowing of food, as well as instability and standing with abducted members. The clinical course was subacute or chronic and in most cases was not fatal. Postmortem examination performed in one already very sick, euthanized animal, did not show significant macroscopic lesions; histopathological examination revealed slight edema and degenerative alterations of a few axons, mainly in the medulla oblongata. In all pastures where horses were affected, plenty of bamboo had been eaten, probably because of scarcity of pasture. By feeding large amounts of fresh bamboo leaves of this region, in different growing stages, to three horses (horse 1, 47 g/kg/d for 30 days; horse 2, 10 g/kg/d for 60 days; horse 3, 18 g/kg on the first day, and 31 g/kg/d for 6 more days)—the animals ate the leaves unassisted—it was possible to reproduce nervous symptoms essentially identical to those observed in the natural disease 24 to 72 hours after the first feeding of the plant. In spite of continuous administration of the plant, intensity of the clinical signs did not increase. Based on field observations and comparison of the clinical and pathological pictures seen in the natural and experimental disease, the described illness can be concluded to be caused by the ingestion of large amounts of the leaves of Bambusa vulgaris f. vulgaris.  相似文献   

Epizootic botulism of cattle in Brazil.     

J D?bereiner  C H Tokarnia  J Langenegger  I S Dutra 《DTW. Deutsche tier?rztliche Wochenschrift》1992,99(5):188-190

The first diagnosis of botulism in cattle in Brazil and its epizootiology are reviewed. The high prevalence of the disease raised on phosphorus deficient pastures in Savanna regions has caused severe economic losses in the past. The temperature induced microcomplement fixation test (TIMCF) confirmed the clinical-pathological diagnosis in all of the 24 cases studied by this method. The most important reason why botulism has not been controlled satisfactorily in Brazil is the lack of an available effective vaccine (type C and D). Additional prophylactic measures are phosphorus supplementation and removal of carcasses from the pasture.  相似文献   

CA15.3, CEA and LDH in Dogs with Malignant Mammary Tumors     

L.C. Campos  G.E. Lavalle  A. Estrela‐Lima  J.C. Melgaço de Faria  J.E. Guimarães  Á.P. Dutra  E. Ferreira  L.P. de Sousa  É.M.L. Rabelo  A.F.D. Vieira da Costa  G.D. Cassali 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2012,26(6):1383-1388

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Behavioral and physiological evaluation of sows raised in outdoors systems in the Brazilian semiarid region     

de Melo  Rafaela Leitão Correia  Dutra Júnior  Wilson Moreira  Palhares  Liliane Olímpio  de Moura Ferreira  Débora Nathália  de Aquino  Rafael Santos  Cordeiro Manso  Helena Emília Cavalcanti 《Tropical animal health and production》2019,51(5):1057-1063

Tropical Animal Health and Production - The objective of this study was to evaluate the biochemical, behavioral, and physiological parameters in pregnant sows of native and improved crossbreeds...  相似文献   

Influence of biofilm on the production of <Emphasis Type="Italic">Farfantepenaeus paulensis</Emphasis> in pens in the Patos Lagoon estuary     

Eduardo Luis Cupertino Ballester  Fabrício Martins Dutra  Tito Luís Pisseti  Ronaldo Olivera Cavalli  Paulo César Abreu  Wilson WasieleskyJr 《Aquaculture International》2018,26(3):713-726

The objective of the present work was to determine the influence of artificial substrates that increase the area for biofilm development on the production performance of Farfantepenaeus paulensis juveniles in pens. Shrimp were stocked at a density of 20/m² in pen structures (bottom area?=?50 m²) that were installed in the Patos Lagoon estuary. Two treatments with three repetitions were analyzed, where artificial substrates (polyethylene nets—1-mm mesh size) were added to increase the area for biofilm development by 100%, and where no substrates were added. During the experimental period, the biomass and the composition of the biofilm were assessed. After 86 days of rearing, no significant differences were found in shrimp performance between the treatments (p?>?0.05). However, the examination of the chlorophyll a, dry weight, and composition of the biofilm indicated that the shrimp were actively consuming the biofilm attached to the artificial substrates. Significant decreases in the abundances of nematodes >?500 μm after the 56th day and of tintinnids and rotifers between day 28 and day 42, indicated that the shrimp were selectively predating on these organisms. Moreover, a decrease in the chlorophyll a concentration in the biofilm suggests that the shrimp were consuming the microalgae. Although the increase in the area for biofilm development did not improve shrimp performance, the shrimp presented the highest growth rates when they consumed most of the biofilm microorganisms.  相似文献   

Antiparasitic efficacy of dietary administration of trichlorfon (Masoten®) in the control of Neoechinorhynchus buttnerae (Neochinorhynchidae) in Colossoma macropomum (Serrasalmidae)     

Castro  Liliane Araújo  Andrade-Porto  Sanny Maria  Oliveira  Rebeca Guimarães  Batista  Yasmin Luzeiro  Silva  Felipe Moura Araújo  Oliveira  Christiane Patrícia Feitosa  Affonso  Elizabeth Gusmão  Cruz  Claudinei  Tavares-Dias  Marcos 《Aquaculture International》2021,29(6):2477-2488

Aquaculture International - This study investigated the efficacy of trichlorfon against Neoechinorhynchus buttnerae, as well as its palatability, residue analysis, and the blood parameters of...  相似文献   

Pseudomonas putida improved soil enzyme activity and growth of kasumbha under low input of mineral fertilizers     

Asia Nosheen  Humaira Yasmin  Rabia Naz  Asghari Bano  Rumana Keyani  Ishtiaq Hussain 《Soil Science and Plant Nutrition》2018,64(4):520-525

Effective management of the nutrients and enzyme activity in the soil is necessary for maximum crop growth and productivity. However, the excessive use of chemical fertilizers (CFs) not only adversely affects the soil nutrient status and soil physicochemical properties but also aids pollution to the ecosystem. The objective of present study was to investigate the effect of single as well as combined applications of phosphate-solubilizing bacteria and agrochemicals on important soil enzyme activities and their impact on the growth of kasumbha (safflower). Pseudomonas putida (P. putida;10⁶ cells/mL) was applied as seed inoculation prior to sowing, and CFs were applied as full, half, and quarter doses during sowing to modulate the growth of kasumbha host plants. P. putida in combination with half dose of CFs (PH) increased the soil urease and phosphatase activities, while P. putida combined with quarter dose of CFs (PQ) augmented the soil invertase activities. Moreover, the PQ treatment exhibited the maximum colony-forming units of P. putida. Leaf chlorophyll, carotenoids, protein contents, and root lengths were increased by PH treatment. Whereas, shoot length and leaf area were improved by PH and PQ treatments, respectively. Leaf protease activity was enhanced by P. putida in combination with full dose of CFs and PQ treatments, while leaf phosphate contents were significantly improved by PQ treatment. It can be concluded that P. putida in combination with half as well as quarter doses of CFs is a promising approach for the improvement of soil enzyme activities and growth of kasumbha and replacing 50% of the use of CFs.  相似文献