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1.
Kaneki K Kanouchi H Matsumoto M Kawasaki Y Akuzawa M Oka T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(7):781-785
Perchloric acid-soluble protein (PSP) is a members of a new hypothetical family (YER057c/YJGF family) of small proteins with presently unknown function. The high degree of evolutionary conservation of these proteins reflect an involvement in basic cellular regulation. The expression of PSP was examined in rat hepatoma cell dRLh 84-beared rats. The tumor weight increased to 4.24 g at 3 weeks after the transplantation of hepatoma cells and hepatoma which has less differentiated characteristics were observed in rat liver. The expression of PSP in rat hepatoma was down regulated as compared with intact tissue. Thus the expression of PSP seems to be associated with the differentiation process in these transformed cells. On the other hand, some positive cells against the PSP-antibody were observed in the central region of tumor tissue by immunohistochemistry. These cells were shown to be the lymphocytes and the macrophages. The involvement of PSP to cellular growth and differentiation is discussed. 相似文献
2.
Uyanga Ariya Ken-Ya Hamano Takushi Makimoto Shu Kinoshita Yasuaki Akaji Yuko Miyazaki Muneto Hirobe Keiji Sakamoto 《Journal of Forest Research》2016,21(2):73-83
Dendrochronological approaches enable us to understand forest stand dynamics by estimation of disturbance history and age structure. The present study was conducted in an old-growth beech forest in a forest reserve in western Japan. Increment cores were taken for tree ring analysis from all canopy trees in a 50 m × 130 m study plot. Radial growth release criteria were developed to identify significant growth releases in each tree ring series and to characterize the disturbance history of the study site. The age structure of the forest was indicative of continuous establishment by Fagus crenata and simultaneous establishment by Magnolia obovata. A variety of low-intensity disturbances were identified in each decade, especially after the 1900s, but the occurrence of high-intensity catastrophic disturbance was rare, and likely played an important role in maintaining species diversity in the existing forest canopy. The results also suggest that F. crenata regenerates gradually before and after both large- and small-scale disturbances, whereas M. obovata and Betula grossa regenerate only after large-scale catastrophic disturbances. 相似文献
3.
Shotaro KUBO Morihiro TATENO Yasuaki ICHIKAWA Yasuyuki ENDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1275-1279
Tick-borne diseases are often encountered in canine clinical practice. In the present
study, a molecular epidemiological survey of dogs in Japan was conducted to understand the
prevalence and geographical distribution of Babesia spp.,
Hepatozoon spp., Ehrlichia spp. and
Anaplasma spp. Pathogen-derived DNA in blood samples obtained from 722
dogs with a history of exposure to ticks and/or fleas was examined by PCR. The prevalence
of Babesia gibsoni, Babesia odocoilei-like species,
Hepatozoon canis and Ehrlichia
spp./Anaplasma spp. was 2.4% (16/722), 0.1% (1/722), 2.5% (18/722) and
1.5% (11/722), respectively. While B. gibsoni and
Ehrlichia spp./Anaplasma spp. were detected in the
western part of Japan, H. canis was detected in Tohoku area in addition
to western and central parts of Japan. 相似文献
4.
Nakamura Y Yoshimoto M Murata Y Shimoishi Y Asai Y Park EY Sato K Nakamura Y 《Journal of agricultural and food chemistry》2007,55(11):4407-4413
In the present study, papaya (Carica papaya) seed and edible pulp were carefully separated and then the contents of benzyl isothiocyanate and the corresponding glucosinolate (benzyl glucosinolate, glucotropaeolin) quantified in each part. The papaya seed with myrosinase inactivation contained >1 mmol of benzyl glucosinolate in 100 g of fresh seed. This content is equivalent to that of Karami daikon (the hottest Japanese white radish) or that of cress. The papaya seed extract also showed a very high activity of myrosinase and, without myrosinase inactivation, produced 460 micromol of benzyl isothiocyanate in 100 g of seed. In contrast, papaya pulp contained an undetectable amount of benzyl glucosinolate and showed no significant myrosinase activity. The n-hexane extract of the papaya seed homogenate was highly effective in inhibiting superoxide generation and apoptosis induction in HL-60 cells, the activities of which are comparable to those of authentic benzyl isothiocyanate. 相似文献
5.
Miyuki Nakajima Shuirong Tang Yasuaki Hori Eiko Yaginuma Satoshi Hattori 《Soil Science and Plant Nutrition》2016,62(1):90-98
Submerged rice paddies are a major source of methane (CH4) which is the second most important greenhouse gas after carbon dioxide (CO2). Accelerating rice straw decomposition during the off-rice season could help to reduce CH4 emission from rice paddies during the single rice-growth season in cold temperate regions. For understanding how both temperature and moisture can affect the rate of rice straw decomposition during the off-rice season in the cold temperate region of Tohoku district, Japan, a modeling incubation experiment was carried out in the laboratory. Bulk soil and soil mixed with 2% of δ13C-labeled rice straw with a full factorial combination of four temperature levels (?5 to 5, 5, 15, 25°C) and two moisture levels (60% and 100% WFPS) were incubated for 24 weeks. The daily change from ?5 to 5°C was used to model the freezing–thawing cycles occurring during the winter season. The rates of rice straw decomposition were calculated by (i) CO2 production; (ii) change in the soil organic carbon (SOC) content; and (iii) change in the δ13C value of SOC. The results indicated that both temperature and moisture affected the rate of rice straw decomposition during the 24-week aerobic incubation period. Rates of rice straw decomposition increased not only with high temperature, but also with high moisture conditions. The rates of rice straw decomposition were more accurately calculated by CO2 production compared to those calculated by the change in the SOC content, or in its δ13C value. Under high moisture at 100% WFPS condition, the rates of rice straw decomposition were 14.0, 22.2, 33.5 and 46.2% at ?5 to 5, 5, 15 and 25°C temperature treatments, respectively. While under low moisture at 60% WFPS condition, these rates were 12.7, 18.3, 31.2 and 38.4%, respectively. The Q10 of rice straw decomposition was higher between ?5 to 5 and 5°C than that between 5 and 15°C and that between 15 and 25°C. Daily freezing–thawing cycles (from ?5 to 5°C) did not stimulate rice straw decomposition compared with low temperature at 5°C. This study implies that to reduce CH4 emission from rice paddies during the single rice-growth season in the cold temperate regions, enhancing rice straw decomposition during the high temperature period is very important. 相似文献
6.
Rie Gappa-Adachi Yasuaki Morita Yoshifumi Shimomoto Shigeharu Takeuchi 《Journal of General Plant Pathology》2014,80(1):103-107
Sweet peppers (cv. Tosajishi beauty) with leaf blight symptoms were observed in Kochi Prefecture, 2003. Initially, small spots formed on leaves, and later enlarged to brown to dark brown spots, and eventually blighted leaves fell. Several bacteria that caused the same symptoms were isolated and subsequently reisolated. These bacteria were identified as Pseudomonas cichorii on the basis of bacterial characteristics and the 16S rRNA gene sequence. This is the first report of bacterial leaf blight in the sweet pepper in the world. 相似文献
7.
Taiko Oda Jong-Soo Lee Yuta Sato Yasuaki Kabe Satoshi Sakamoto Hiroshi Handa Remy E. P. Mangindaan Michio Namikoshi 《Marine drugs》2009,7(4):589-599
N,N-Didesmethylgrossularine-1 (DDMG-1), a compound with a rare α-carboline structure, was isolated from an Indonesian ascidian Polycarpa aurata as responsible for the observed inhibitory activity against TNF-α production in lipopolysaccharide-stimulated murine macrophage-like RAW264.7 cells. DDMG-1 inhibited the mRNA level of mTNF-α, IκB-α degradation, and binding of NF-κB to the target DNA site in LPS-stimulated RAW 264.7 cells. Moreover, DDMG-1 had an inhibitory effect on the production of IL-8, which is produced in CD14+-THP-1 cells stimulated by LPS. DDMG-1 is thus a promising drug candidate lead compound for the treatment of chronic inflammatory diseases, such as rheumatoid arthritis. 相似文献
8.
9.
The external layer of a teleost fish scale is composed of type I collagen, an amorphous matrix substance and hydroxyapatite
crystals. Calcification of this layer can be inhibited in the scale-regenerating process under calcium- and phosphate-deficient
(CaDPD) conditions, and can be facilitated by incubation in physiological saline. The aim of this study was to evaluate this
model of calcification using histological and quantitative analysis in order to promote further understanding of the mechanism
of calcification in fish scales. We found that the external layer of the scales produced under CaDPD conditions contained
more densely aligned collagen fibrils with a small amount of the amorphous matrix substance. The CaDPD scale contained only
one-third of the amount of calcium and phosphate present in the control fish. After 4 hours of incubation, a two- to threefold
increase in calcium content and a 1.5-fold increase in phosphate content were observed. Calcification proceeded in the external
layer, and mineral deposits grew only in the electron-dense matrix substance. We conclude that this model would be suitable
for studying the early process of fish scale calcification that occurs in the noncollagenous substance. The electron-dense
substance may contain key molecules that promote calcification. 相似文献
10.