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1.
Lee AW Chen TL Shih CM Huang CY Tsao NW Chang NC Chen YH Fong TH Lin FY 《Journal of agricultural and food chemistry》2010,58(24):12941-12949
Ursolic acid (UA), a triterpenoid compound found in plants, is used in the human diet and in medicinal herbs and possesses a wide range of biological benefits including antioxidative, anti-inflammatory, and anticarcinogenic effects. Endothelial expression of allograft inflammatory factor-1 (AIF-1) mediates vasculogenesis, and nitric oxide (NO) produced by endothelial NO (eNOS) represents a mechanism of vascular protection. It is unclear whether UA affects the neovascularization mediated by AIF-1 and eNOS expression. This study investigated the effects and mechanisms of UA on angiogenesis in vivo in hind limb ischemic animal models and in vitro in human coronary artery endothelial cells (HCECs). This study explored the impact of UA on endothelial cell (EC) activities in vitro in HCECs, vascular neovasculogenesis in vivo in a mouse hind limb ischemia model, and the possible role of AIF-1 in vasculogenesis. The results demonstrate that UA enhances collateral blood flow recovery through induction of neovascularization in a hind limb ischemia mouse model. In vitro data showed that UA increases tube formation and migration capacities in human endothelial cells, and exposing HCECs to UA increased AIF-1 expression through a NO-related mechanism. Moreover, UA administration increased capillary density and eNOS and AIF-1 expression in ischemic muscle. These findings suggest that UA may be a potential therapeutic agent in the induction of neovascularization and provide a novel mechanistic insight into the potential effects of UA on ischemic vascular diseases. 相似文献
2.
Hsu CL Lin W Seshasayee D Chen YH Ding X Lin Z Suto E Huang Z Lee WP Park H Xu M Sun M Rangell L Lutman JL Ulufatu S Stefanich E Chalouni C Sagolla M Diehl L Fielder P Dean B Balazs M Martin F 《Science (New York, N.Y.)》2012,335(6064):89-92
Lysosomal storage diseases (LSDs) are a group of heterogeneous disorders caused by defects in lysosomal enzymes or transporters, resulting in accumulation of undegraded macromolecules or metabolites. Macrophage numbers are expanded in several LSDs, leading to histiocytosis of unknown pathophysiology. Here, we found that mice lacking the equilibrative nucleoside transporter 3 (ENT3) developed a spontaneous and progressive macrophage-dominated histiocytosis. In the absence of ENT3, defective apoptotic cell clearance led to lysosomal nucleoside buildup, elevated intralysosomal pH, and altered macrophage function. The macrophage accumulation was partly due to increased macrophage colony-stimulating factor and receptor expression and signaling secondary to the lysosomal defects. These studies suggest a cellular and molecular basis for the development of histiocytosis in several human syndromes associated with ENT3 mutations and potentially other LSDs. 相似文献
3.
Yu-Ru Huang Yung-Hsiang Tsai Ching-Lu Liu Wei-Zong Syue Yi-Cheng Su 《Journal Of Aquatic Food Product Technology》2018,27(2):132-143
Proximate compositions of dorsal, ventral, leg, and tail cuts of farmed spectacled caiman (Caiman crocodilus) were determined. The water, protein, fat, and ash contents of muscle were 77–78%, 19–23%, 0.3–1.2%, and 1.0%, respectively. The dominant nucleotide-related compound in the tissues was inosine monophosphate (IMP) (68.6–84.9%). The predominant free amino acids (FAAs) in the muscle of spectacled caiman were taurine (Tau), glycine (Gly), alanine (Ala), and histidine (His), along with a dipeptide of anserine (Ans) (80–183 mg/100 g) and carnosine (Car) (35–103 mg/100 g) being detected in each sample. The major fatty acid composition included palmitic (16:0), oleic (18:1 cis-9), linoleic (18:2n-6), and stearic (C18:0) acids. The amount of docosahexaenoic acid (4.27–8.76%) was higher than that of eicosapentaenoic acid (0.99–2.24%). The ratio of polyunsaturated fatty acid/saturated fatty acid in spectacled caiman (0.83–1.19) was higher than that in other commonly consumed meat. Meat from the spectacled caiman can be characterized as a valuable food for human consumption in terms of these nutritional features. 相似文献
4.
Chung-Saint Lin Tser-Sheng Lin Din-Yuan Yu Yi-Cheng Su Yung-Hsiang Tsai 《Journal Of Aquatic Food Product Technology》2013,22(8):1301-1310
ABSTRACTVibrio parahaemolyticus is a human pathogen frequently found in seafood. Once the seafood is contaminated by V. parahaemolyticus, it can become a vehicle for foodborne illness. The conventional culture methods for detection of V. parahaemolyticus are time-consuming and cannot differentiate pathogenic strains from nonpathogenic ones. In this study, a multiplex polymerase chain reaction (PCR) technique was investigated for detecting tdh, chiA, and toxR of V. parahaemolyticus. The sensitivity of the multiplex PCR was determined by testing 28 strains of V. parahaemolyticus, 15 non-V. parahaemolyticus strains, and fresh seafood spiked with cells of V. parahaemolyticus. All the strains were analyzed for production of thermostable direct hemolysin (TDH) and chitinase. This study showed that both the chiA and toxR are excellent markers for detecting V. parahaemolyticus strains, and a multiplex PCR targeting chiA and tdh genes can be applied to simultaneously detect environmental and pathogenic V. parahaemolyticus. 相似文献
5.
Hui-Hua Hsiao Tien-Chiu Wu Yung-Hsiang Tsai Chia-Hung Kuo Ren-Han Huang Yong-Han Hong Chun-Yung Huang 《Marine drugs》2021,19(4)
Intensive efforts have been undertaken in the fields of prevention, diagnosis, and therapy of lung cancer. Fucoidans exhibit a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan. The determination of oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades is challenging. In this investigation, we used a previously developed fucoidan (SCA), which served as a native fucoidan, to generate two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2). SCA, SCA-S1, and SCA-S2 showed differences in compositions and had the characteristic structural features of fucoidan by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses. The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades. Our findings indicate that the oversulfation of fucoidan promotes apoptosis of lung cancer cells and the mechanism may involve the Akt/mTOR/S6 pathway. Further in vivo research is needed to establish the precise mechanism whereby oversulfated fucoidan mitigates the progression of lung cancer. 相似文献
6.
Lin FY Chen YH Chen YL Wu TC Li CY Chen JW Lin SJ 《Journal of agricultural and food chemistry》2007,55(5):1977-1984
Toll-like receptor 4 (TLR4) initiates the inflammatory response in blood vessels in reaction to immune stimuli such as lipopolysaccharide (LPS) produced by gram-negative bacteria. LPS-induced proliferation and functional perturbation in vascular smooth muscle cells play important roles during atherogenesis. Ginkgo biloba extract is an antiatherothrombotic Chinese herbal medicine with anti-inflammatory properties. The effects of G. biloba extract on LPS-induced proliferation and TLR4 expression and the underlying mechanisms for these actions, in human aortic smooth muscle cells (HASMCs), were examined in vitro. LPS-induced proliferation was mediated by the expression of TLR4 in HASMCs. LPS increased the expression of TLR4 in HASMCs, and this effect was mediated by the activation of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, phosphorylation of intracellular mitogen-activated protein kinases (MAPKs), and increases in the cytoplasmic level of HuR and TLR4 mRNA stability. G. biloba extract inhibited LPS-induced HASMC proliferation and decreased the expression of TLR4 by inhibiting LPS-induced NADPH oxidase activation, mRNA stabilization, and MAPK signaling pathways. These results suggest that LPS-induced TLR4 expression contributes to HASMC proliferation and that G. biloba inhibits LPS-stimulated proliferation of HASMCs by decreasing TLR4 expression. 相似文献
7.
Wei-Cheng Shiao Tien-Chiu Wu Chia-Hung Kuo Yung-Hsiang Tsai Mei-Ling Tsai Yong-Han Hong Chun-Yung Huang 《Marine drugs》2021,19(5)
Fish gelatin and its hydrolysates exhibit a variety of biological characteristics, which include antihypertensive and antioxidant properties. In this study, fish gelatins were extracted from extrusion-pretreated tilapia scales, and then subjected to analyses to determine the physicochemical properties and antioxidant activity of the extracted gelatins. Our findings indicate that TSG2 (preconditioned with 1.26% citric acid) possessed the greatest extraction yield, as well as higher antioxidant activities compared with the other extracted gelatins. Hence, TSG2 was subjected to further hydrolyzation using different proteases and ultrafiltration conditions, which yielded four gelatin hydrolysates: TSGH1, TSGH2, TSGH3, and TSGH4. The results showed that TSGH4 (Pepsin + Pancreatin and ultrafiltration < 3000 Da) had a higher yield and greater antioxidant activity in comparison with the other gelatin hydrolysates. As such, TSGH4 was subjected to further fractionation using a Superdex peptide column and two-stage reverse-phase column HPLC chromatography, yielding a subfraction TSGH4-6-2-b, which possessed the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity compared with the other fractions. Further LC-ESI/MS/MS analysis of TSGH4-6-2-b suggested two novel peptides (GYDEY and EPGKSGEQGAPGEAGAP), which could have potential as naturally-occurring peptides with antioxidant properties. These promising results suggest that these antioxidant peptides could have applications in food products, nutraceuticals, and cosmetics. 相似文献
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9.
Chen WC Lin WY Chen HY Chang CH Tsai FJ Man KM Shen JL Chen YH 《Journal of agricultural and food chemistry》2012,60(10):2753-2757
Melamine-tainted food can induce renal stones in both humans and animals. We have previously reported a novel Drosophila model for the study of renal stone disease. In addition to hyperoxaluria-causing agents, we also tested herein the effect of melamine on crystal formation in Drosophila . The results indicate that administration of melamine alone caused crystal formation in a dose-dependent manner. The crystals also appeared after ingestion of melamine for 3 weeks in the Malpighian tubules of Drosophila when viewed with polarized light. Administration of potassium citrate (K citrate) was found to significantly ameliorate the melamine-induced reduction of lifespan. However, administration of K citrate failed to reduce the quantity of crystals. Because calcium oxalate is not the major crystal induced by melamine, the predominant components of melamine-induced crystals and the potential crystal inhibitors warrant further investigation. 相似文献
10.
Yih-Mon?Jaw Yun-Yun?Chen Yi-Chen?Lee Pei-Hsia?Lee Chii-Ming?Jiang Yung-Hsiang?TsaiEmail author 《Fisheries Science》2012,78(1):155-162
Forty fish meals and five fish soluble concentrates (FSC) imported to Taiwan were sampled to determine biogenic amines and
histamine-forming bacteria. Average levels of water activity (0.8), water content (39.9%), salt content (7.32%), and total
volatile basic nitrogen (TVBN) (150.7 mg/100 g) in FSC samples were significantly higher than those of fish meal samples,
whereas the average levels of crude fat (3.94%) and crude protein (40.7%) in FSC samples were lower than those of fish meal
samples. Thirty-five (87.5%) fish meal samples and 5 (100%) FSC samples had TVBN levels above the decomposition limit level
of 30 mg/100 g in fresh fish meat. Average contents of putrescine, cadaverine, histamine, and tyramine in tested samples were
higher than those of tryptamine, 2-phenylamine, spermidine, and spermine. Four fish meal samples (10%, 4/40) and 1 FSC sample
(20%, 1/5) had histamine levels >20 mg/100 g. Five histamine-producing bacterial strains isolated from fish meal samples producing
1.31–6.21 ppm of histamine in trypticase soy broth supplemented with 1.0% l-histidine (TSBH) were identified as Bacillus licheniformis (three strains), B. amyloliquefaciens (one strain), and B. subtilis (one strain). 相似文献