Probiotics have been widely used in aquaculture worldwide especially in China as antibiotics have been banned in aquaculture. In the present study, 27 potential strains were isolated from Rhynchocypris lagowskii Dybowski intestinal tract towards the assessment of their probiotic potential. Two potential probiotics were finally screened from the 27 candidate strains according to the results of enzyme-producing ability, in vitro against pathogens ability and antibiotic sensitivity test. The 2 potential probiotics were identified and confirmed on the basis of their colony morphology, biochemical characteristics, and 16S rRNA sequencing. The probiotic strains, LSG2-3–2 and LSG3-6, were identified to be Bacillus methylotrophicus and B. tequilensis, respectively. Further studies showed that LSG2-3–2 and LSG3-6 had excellent tolerance to high temperature (80℃), low pH (3–5), bile salts (0.3%), intestinal juice (10%), and gastric juice (0.5%). The adhesion rates of LSG2-3–2 and LSG3-6 in the in vitro intestinal mucosal adhesion model were 17.74% and 24.04%, respectively. Analysis of their bacteria surface adhesive proteins revealed that the lectins on the bacterial surface of LSG2-3–2 and LSG3-6 were mainly protein and glycoprotein properties, respectively. The adhesion receptor components in the mucus proteins of the two strains were all protein properties. The results of the inhibitory adhesion test indicated that LSG3-6 had a higher inhibitory effect on Aeromonas hydrophila and LSG2-3–2 had a better inhibitory effect on A. veronii. The biosafety assay confirmed that the isolates were not pathogenic to the host fish. Based on the presently observed characteristic features, it can be concluded that LSG2-3–2 (B. methylotrophicus) and LSG3-6 (B. tequilensis) retrieved from the digestive tract of R. lagowskii can be used for the healthy breeding and disease prevention of R. lagowskii.
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Zhang Yurou Wu Zhenchao Yu Mengnan Zhang Dongming Wang Qiuju Lin Lili Wang Guiqin Elsadek Mahmoud M. Yao Qi Chen Yuke Guo Zhixin 《Aquaculture International》2022,30(2):747-772
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以萝卜(Raphanus sativus L.)为试验材料,采用室内水培试验,研究不同浓度钴(CoCl2·6H2O)(0.01、0.05、0.10、0.50、1.00 mmol·L~(-1))对80 mmol·L~(-1)NaCl胁迫下萝卜种子萌发和幼苗抗氧化能力的影响,为盐渍地萝卜栽培提供参考依据。结果表明:在NaCl胁迫下添加不同浓度钴后,萝卜种子发芽率、发芽势、发芽指数及活力指数均显著高于NaCl胁迫处理。NaCl胁迫的幼苗丙二醛(MDA)含量,超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性显著增加,添加0.01~0.10 mmol·L~(-1)外源钴有利于增加萝卜幼苗SOD、过氧化物酶(POD)及CAT活性,降低MDA含量。但添加0.50~1.00 mmol·L~(-1)外源钴则降低SOD及CAT活性,提高MDA含量。综上所述,适当浓度(0.01~0.10 mmol·L~(-1))的钴能降低NaCl胁迫对萝卜种子萌发的抑制作用,提高幼苗抗氧化酶活性,降低MDA累积量,其中,0.10 mmol·L~(-1)外源钴对NaCl胁迫的作用效果最好,而较高浓度(0.50~1.00 mmol·L~(-1))的外源钴反而对萝卜幼苗产生毒害作用。 相似文献
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为开发高品质的益生菌发酵复合果蔬汁,对果蔬汁的发酵工艺及风味品质进行研究.通过观测发酵前后的pH值变化、滴定酸度、糖度和活菌数等因素,研究不同的发酵微生物、不同的果蔬种类和不同发酵时间对发酵结果的影响,结合感官评价和气相色谱-质谱联用法(GC-MS)对风味品质进行评价分析.果蔬种类和发酵菌种单因素试验结果表明,选择芒果、圣女果和胡萝卜为复合果蔬汁原料,以植物乳杆菌、副干酪乳杆菌和瑞士乳杆菌复配发酵48 h的制品pH低、酸度较高、活菌数高、感官评价结果质量最优;GC-MS风味分析表明,发酵过程中酯类、醇类和酸类的挥发性风味物质相对增加,风味较好.说明以优化的发酵工艺制备的复合果蔬汁具有感官风味较好、活菌数含量高等特点,可为发酵制品的研制及发酵果蔬汁的市场开拓提供参考. 相似文献
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为改善干热河谷地区咖啡园脆弱的生态环境,探讨不同的覆盖作物种植模式对咖啡园土壤质量的影响,本研究通过设置紫花苜蓿单播（A1）,紫花苜蓿×光叶紫花苕混播（A2）两个处理,以休闲处理（CK）为对照,分析了覆盖作物盛花期时对土壤酶活、理化性质、细菌群落结构和多样性的影响。结果表明：A1、A2处理都能显著提高咖啡园土壤有机质和氮素含量（P<0.05）,促进土壤pH从弱碱性恢复到中性,更利于咖啡树的生长;与CK相比,A1的土壤脲酶显著提高（P<0.05）,A1、A2、CK的土壤过氧化氢酶活性无显著差异（P>0.05）;A1的Sobs指数、Chao1指数显著低于A2、CK处理（P<0.05）,A1、A2的Shannon指数显著低于CK（P<0.05）,与休闲处理相比,单播/混播覆盖作物种植降低了土壤细菌多样性,但紫花苜蓿×光叶紫花苕混播的土壤细菌多样性比紫花苜蓿单播更丰富;酸杆菌门是A1的优势菌门,放线菌门是A2、CK组的优势菌门,酸杆菌门的RB41是3个处理的优势菌属;pH值对土壤细菌种群分布的影响最大;细菌群落的主要功能是碳水化合物代谢和氨基酸代谢。覆盖作物种植可以显著增加干热河谷地区咖啡园土壤营养,调整土壤酸碱度,影响土壤细菌群落结构和功能,土壤改良效果显著,紫花苜蓿和光叶紫花苕混播是干热河谷地区咖啡园覆盖作物种植的较优模式。 相似文献
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