High genetic relatedness among Mycobacterium avium strains isolated from pigs and humans revealed by comparative IS1245 RFLP analysis     

Tirkkonen T  Pakarinen J  Moisander AM  Mäkinen J  Soini H  Ali-Vehmas T 《Veterinary microbiology》2007,125(1-2):175-181

Members of the Mycobacterium avium complex cause pig mycobacteriosis and opportunistic human infections. Infections due to environmental mycobacteria are increasing in both industrial and developing countries. Mycobacterium-infected pig carcasses can pass for human consumption due to the poor specificity of meat control by visual detection at the slaughter houses. The genetic relatedness of porcine and human MAC isolates in Finland has been unknown. M. avium isolates isolated from pig organs (n=16) and clinical samples (n=13) were compared by IS1245 RFLP analysis to evaluate the similarity of the isolates obtained from human and porcine samples. Nearly identical multicopy M. avium subsp. hominissuis IS1245 RFLP fingerprints were obtained for isolates of porcine and human origin. IS1245 RFLP patterns of 38% of the porcine and human M. a. hominissuis isolates were >90% similar. The RFLP patterns of two porcine and two human isolates showed >95% similarity. The high similarity of the IS1245 RFLP patterns of the human and porcine M. a. hominissuis isolates indicates close genetic relatedness, suggesting that M. a. hominissuis is transmitted between pigs and humans, or that pigs and humans share common environmental sources of infection. Porcine and human isolates with RFLP patterns differing by only one or two bands were found, which shows that the same M. a. hominissuis strains may infect both humans and pigs.  相似文献   

Embryonic development in the blue fox     

M Valtonen  W A King  I Gustavsson  A M?kinen 《Nordisk veterinaermedicin》1985,37(4):243-248

Embryos from vixens at different stages of gestation were measured and photographed. Implantation was observed 16-18 days after breeding. Differentiation and the development of characteristic features were described and embryonic loss was studied in relation to number of corpora lutea.  相似文献   

MED12, the mediator complex subunit 12 gene, is mutated at high frequency in uterine leiomyomas     

Mäkinen N  Mehine M  Tolvanen J  Kaasinen E  Li Y  Lehtonen HJ  Gentile M  Yan J  Enge M  Taipale M  Aavikko M  Katainen R  Virolainen E  Böhling T  Koski TA  Launonen V  Sjöberg J  Taipale J  Vahteristo P  Aaltonen LA 《Science (New York, N.Y.)》2011,334(6053):252-255

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Extreme neutral genetic and morphological divergence supports classification of Adriatic three-spined stickleback (Gasterosteus aculeatus) populations as distinct conservation units     

Jos Manuel Cano  Hannu Sakari Mkinen  Tuomas Leinonen  Jrg Freyhof  Juha Meril 《Biological conservation》2008,141(4):1055-1066

The fact that the intraspecific genetic differentiation in neutral genetic markers and genes coding for adaptive traits are not typically correlated has caused a great deal of conceptual and practical trouble in delimitation of conservation units. Although the importance of combining information on adaptive genetic divergence with information on historical and recent gene flow in the delimitation of conservation units has been recognized, integrated empirical studies to this end are still rare. We explored the evidence for the specific conservation status of two freshwater three-spined stickleback (Gasterosteus aculeatus) populations on the Adriatic side of the Balkan Peninsula by comparing their phenotypic and genetic characteristics to those of other representative European populations. Apart from focusing on the neutral genetic divergence in mitochondrial DNA sequences and microsatellite markers, we also compared the patterns of morphological differentiation (i.e. bony armour development) resulting from adaptation to freshwater environments. The Balkanic populations formed two distinct groups with regard to neutral genetic variation and had the least developed bony armour of all the examined populations. All morphometric analyses identified the two Balkanic populations as phenotypically – and hence most likely also ecologically – clearly distinct from other European three-spined stickleback populations. These results suggest that the two Balkanic populations (River Neretva and River Zeta) fulfil the most stringent criteria (i.e. lack of genetic and ecological exchangeability) to be classified as conservation units distinct from other European three-spined stickleback populations.  相似文献   

Predicting lumber grade and by-product yields for Scots pine trees     

Henna T. Lyhykäinen  Harri Mäkinen  Annikki Mäkelä  Sami Pastila  Antti Heikkilä  Arto Usenius 《Forest Ecology and Management》2009

The purpose of this study was to develop models for estimating yields of lumber grades and by-products of individual Scots pine (Pinus sylvestris L.) trees using stem and crown dimensions as explanatory variables. Two separate data sets were used: (1) one simulated by the process-based growth model, PipeQual, which provides information about stem form and branch properties. The model was used to predict the 3D structure of Scots pine stems from thinning regimes of varying intensity and rotation periods and (2) an empirical data set with detailed 3D measurements of stem structure. The stems were sawn using the WoodCim sawing simulator and the yields and grades of the individual sawn pieces, as well as by-products, were recorded. The sawn timber was classified on A, B, C and D-grades for side and centre boards separately (Nordic Timber grading). By-products were pulpwood, sawmill chips, sawdust and bark.  相似文献   

Effect of tree-level airborne laser-scanning measurement accuracy on the timing and expected value of harvest decisions     

Markus Holopainen  Antti Mäkinen  Jussi Rasinmäki  Juha Hyyppä  Hannu Hyyppä  Harri Kaartinen  Risto Viitala  Mikko Vastaranta  Annika Kangas 《European Journal of Forest Research》2010,129(5):899-907

The objective was to compare tree-level airborne laser-scanning (ALS) data accuracy with standwise estimation data accuracy as input data for forest planning, using tree- and stand-level simulators. The influence of the input data accuracy was studied with respect to (1) timing of the next thinning or clear-cutting and (2) the relative variation in the predicted income of the next logging expressed as the net present value (NPV). The timing and predicted NPV of thinning and clear-cutting operations were considered separately. The research was based on Monte Carlo simulations carried out with the tree- and stand-level simulators using a simulation and optimisation (SIMO) framework. The simulations used treewise measurements taken on 270 circular plots measured at the Evo Field Station, Finland, as input data. Deviations in the tree data measured were generated according to the mean standard errors found in standwise field estimation and tree-level ALS. The accuracy factors of ALS individual tree detection were based on the EUROSDR/ISPRS Tree Extraction Project. The results show that input data accuracy significantly affects both the timing and relative NPV of loggings. Tree-level ALS produces more accurate simulation results than standwise estimation with the error levels assumed. Diameter-based characteristics are the most important input data in all simulations. Accurate tree height estimates cannot be fully utilised in current simulators.  相似文献   

Propagating the errors of initial forest variables through stand- and tree-level growth simulators     

Antti Mäkinen  Markus Holopainen  Annika Kangas  Jussi Rasinmäki 《European Journal of Forest Research》2010,129(5):887-897

Developments in the field of remote sensing have led to various cost-efficient forest inventory methods at different levels of detail. Remote-sensing techniques such as airborne laser scanning (ALS) and digital photogrammetry are becoming feasible alternatives for providing data for forest planning. Forest-planning systems are used to determine the future harvests and silvicultural operations. Input data errors affect the forest growth projections and these effects are dependent on the magnitude of the error. Our objective in this study was to determine how the errors typical to different inventory methods affect forest growth projections at individual stand level during a planning period of 30 years. Another objective was to examine how the errors in input data behave when different types of growth simulators are used. The inventory methods we compared in this study were stand-wise field inventory and single-tree ALS. To study the differences between growth models, we compared two forest simulators consisting of either distance-independent tree-level models or stand-level models. The data in this study covered a 2,000-ha forest area in southern Finland, including 240 sample plots with individually measured trees. The analysis was conducted with Monte Carlo simulations. The results show that the tree-level simulator is less sensitive to errors in the input data and that by using single-tree ALS data, more precise growth projections can be obtained than using stand-wise field inventory data.  相似文献   

Effect of wide spacing on increment and branch properties of young Norway spruce     

Harri Mäkinen  Sebastian Hein 《European Journal of Forest Research》2006,125(3):239-248

The effects of stand density on increment and branch properties were studied in three spacing experiments of Norway spruce [Picea abies (L.) Karst.]. The stand densities ranged from 350 stems ha⁻¹, regarded as open-grown trees, up to 1,600 stems ha⁻¹, corresponding to the density recommended for forestry practice. Properties of all the branches were measured from the stem apex downwards. The study material included a total of 5,661 branches from 45 trees. Increasing stand density resulted in a decrease in radial increment as well as shorter and narrower crowns, but it had no effect on height increment. The average number of spike knots per tree was 0.87, 0.27, and 0.33 in densities of 350, 700 and 1,600 ha⁻¹, respectively. Additionally, in the widely spaced stands of 350 stems ha⁻¹, the fraction of trees having spike knots was high (over 50%). At a density of 1,600 ha⁻¹, the sample trees had somewhat less branches in a whorl compared with the more widely spaced plots. The most pronounced effect of stand density was the increase in branch diameter with decreasing stand density. At a density of 350 ha⁻¹, the maximum branch diameter of all the sample trees exceeded the diameter limit of quality class B in the European quality requirements for round wood. The results give some indication that trees subjected to severe competition would produce smaller branches per unit of crown projection area. However, the possibilities for reducing branch dimensions relative to stem and crown size through competition appear quite restricted.  相似文献   

Two cases of reciprocal translocations in domestic pigs producing small litters     

Mäkinen A  Pitkänen T  Andersson M 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》1997,114(1-6):377-384

SUMMARY: Two boars of Finnish Yorkshire breed were selected for chromosomal analysis because of their small litters. One had a complex three breakpoint reciprocal translocation where the small distal chromosome segments were fused in new combinations between autosomes 2,9 and 14. This boar with a 2n = 38,XY,t(2;9;14)(q23;q22;q25) karyotype had produced approximately 3.3 piglets per litter and its fertility was reduced by 70% compared to average of litter size in Finnish Yorkshire breed pigs. In another Finnish Yorkshire breed boar, the long arm of autosome 7 had broken and attached to the terminal end of autosome 15. The boar had a 2n = 38,XY, rcp(7q-;15q+)(q24;q26) karyotype. The average of piglets per litter sired by this boar was 5.2 and the litter size was reduced by approximately 50%. The chromosomal rearrangements t(2;9;14) was inherited by four of the boar's offspring studied from two whole litters having seven piglets altogether. Meiotic chromosomes were analysed conventionally from one male translocation carrier offspring of the t(2;9;14) boar. Total sperm count and progressive motility of spermatozoa were within normal limits in one male translocation carrier offspring of the t(2;9;14) boar and in the rcp(7q-;15q+) boar. The two reciprocal translocations seem to have arisen spontaneously in these two boars. ZUSAMMENFASSUNG: Zwei F?lle reziproker Translokationen bei Ebern mit kleinen Nachkommen Würfen Von zwei Finnischen Yorkshire Ebern hatte einer drei komplexe reziproke Translokationen, wo kurze distale Chromosomensegmente zu neuen Kombinatioenen zwischen Autosomen 2, 9 und 14 fusioniert waren. Der Eber mit 2n = 38,XY,t(2;9;14)(q23;q22;q25) Karyotyp hatte Nachkommenwürfe mit etwa 3.3 Ferkel weniger und 70% Fruchtbarkeit der bei dieser Rasse normalen. In einem anderen Eber war der lange Arm von Chromosom 7 gebrochen und am Ende von Chromosom 15: 2n = 38,XY, rcp(7q-; 15q+)(q24;q26) Karyotyp. Nachkommenwurfgr??e war 5.2. Das chromosomale Rearrangement t(2;9;14) wurde an 4 Nachkommen in zwei Würfen mit zusammen 7 Ferkel vererbt. Meiotische Chromosomen wurden bei einem m?nnlichen Translokationstr?ger dieser Nachkommen analysiert. Gesamtspermienenzahl und ihre Vorw?rtsbewegung waren in normalen Grenzen bei diesem Nahkommen ebenso wie beim rcp(7q-;15q+) Eber. Die zwei reciproken Translokationen scheinen spontan entstanden zu sein.  相似文献   

X chromosome detection in an XO mare using a human X paint probe, and PCR detection of SRY and amelogenin genes in 3 XY mares     

Mäkinen A  Suojala L  Niini T  Katila T  Tozaki T  Miyake Y  Hasegawa T 《Equine veterinary journal》2001,33(5):527-530

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