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This experiment was conducted to investigate the effects of dietary supplemented with combined yeast and Bacillus coagulans fermentation culture on intestinal morphology, intestinal permeability, cecal fer⁃ mentation and microflora of Rex rabbits. A total of 160 healthy Rex rabbits weaned at 35⁃day⁃old with similar body weight were randomly allocated to 4 groups with 8 replicates per group and 5 rabbits per replicate. Rex rabbits in the control group were fed a basal diet, while the others in experimental groups were fed experimental diets supplemented with 0.01%, 0.02% and 0.04% combined yeast and Bacillus coagulans fermentation cul⁃ ture based on the basal diet, respectively. The pretrial period lasted for 7 days and formal period lasted for 56 days. The results showed as follows: 1) the crypt depth of jejunum in the 0.02% and 0.04% added groups was significantly lower than that in the control group (P<0.05), the jejunum villus height / crypt depth ratio in the 0.02% added group was significantly higher than that in the control group (P < 0. 05), and the ileum villus height in the 0.04% added group was significantly higher than that in the control group (P<0.05) . 2) The ac⁃ tivity of diamine oxidase (DAO) in serum of 0.01% and 0.02% added groups was significantly lower than that in the control group (P<0.05), but there was no significant difference in the content of D⁃lactic acid (D⁃LA) in serum among all groups (P>0.05). 3) There were no significant differences in the pH of jejunum, ileum and cecum among all groups (P>0.05). There were no significant differences in the concentrations of acetic acid, propionic acid, butyric acid and total volatile fatty acid and the ratios of acetic acid, propionic acid and butyric acid in cecum among all groups (P>0.05). 4) Compared with the control group, the Simpson index of 0.02% and 0.04% added groups showed an increasing trend (0.05≤P<0.10). At the phylum level, the rela⁃ tive abundance of Verrucomicrobia showed a trend of decreasing first and then increasing with the combined yeast and Bacillus coagulans fermentation culture increasing (0.05≤P<0.10); the relative abundance of Te⁃ nericutes in the 0.02% added group was significantly higher than that in the 0.01% and 0.04% added groups (P<0.05), but there was no significant difference between the added groups and the control group (P>0.05). At the genus level, the relative abundance of Ruminococcaceae_UCG⁃014, Akkermansia, dgA⁃11_gut_group, Paramuribaculum and Subdoligranulum showed a trend of change with the combined yeast and Bacillus coagu⁃ lans fermentation culture increasing (0.05≤P<0.10). In summary, dietary supplemented with combined yeast and Bacillus coagulans fermentation culture can improve the intestinal morphology and reduce intestinal perme⁃ ability, but has no significant effects on the pH of jejunum, ileum and cecum and cecum fermentation, and can improve the microbial diversity of cecum of Rex rabbits. The recommended addition level is 0.02% to 0.04% based on the previous results of growth performance. © 2023 Chinese Journal of Clinical Research. All rights reserved. 相似文献
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本试验旨在探究泌乳期母兔乳中微生物群落组成与多样性变化。选用3胎次妊娠母兔12只，分别于泌乳第0天（分娩后0.5 h内，L0）、第3天（L3）、第7天（L7）、第21天（L21）、第35天（L35），取母兔新鲜乳汁进行16S rDNA测序和生物学信息分析。结果显示：1) alpha多样性方面，Chao1和Observed＿species指数表现为L0、L21极显著高于L7(P<0.01),L3显著高于L7(P<0.05);Shannon指数表现为L0显著高于L7(P<0.05),L21极显著高于L3、L7(P<0.01);Simpson指数表现为L21显著高于L7(P<0.05)。2)beta多样性方面，L0、L21、L35的微生物组成相似。3）在门水平上，L7的厚壁菌门（Firmicutes）相对丰度极显著低于L0、L35(P<0.01)，显著低于L21(P <0.05);L7的拟杆菌门（Bacteroidetes）相对丰度极显著低于L3、L21(P<0.01)，显著低于L0(P<0.05);L7的变形菌门（Proteobacte... 相似文献
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