羊生长激素的提取及生物化学和生物活性的测定     

郭亚平  夏国良  杨传任 《中国农业大学学报》1990,(4):442-446

600 g 冰冻羊垂体经过酸性溶液提取、硫酸铵的分级分离和 DEAE-纤维素柱层析得纯品(1),其得率为0.08g/kg,最后将部分纯品(1)过 Sephadex G-100分子筛,得到高纯的羊生长激素[纯品(2)]。提取的羊生长激素具有相当高的理化均一性,纯品(2):聚丙烯酰胺凝胶电泳呈现一条主带和二条酸性小带;聚丙烯酰胺等电聚焦电泳呈现二条带,其等电点分别为 pH7.35和 pH7.40,SDS—电泳呈现二条很难分开的带,其平均分子量为20 100道尔顿;用DNS-CI 法测得的 N-末端氨基酸是2个,丙氨酸和苯氨酸。纯品(1)理化均一性稍差:SDS—电泳较纯品(2)多出两条分子量低的带;等电聚焦电泳多出六条酸性小带。除此之外,其他理化性质同纯品(2)。用大白鼠胫骨实验测定生物活性,测得纯品(1)可使垂体大白鼠胫骨软骨盘的宽度相对增加79%。未对纯品(2)进行生物活性测定。用羊催乳素放射免疫药盒测定,纯品(1)和纯品(2)与羊催乳素均无交叉反应。  相似文献   

Cloning of plasma membrane H-ATPase gene in Populus euphratica Oliv.     

Ning De-juan Hou Pei-chen Hu Zan-min Shen Xin Chen Shao-liang College of Biological Sciences  Biotechnology  Beijing Forestry University  Beijing  P. R. China Institute of Genetics  Developmental Biology  Chinese Academy of Sciences  Beijing  P. R. China 《中国林学(英文版)》2006,8(4):15-19

For this paper, the plasma membrane (PM) H -ATPase gene has been cloned from Populus euphratica Oliv. through a homology based strategy. The isolated 3,210 bp cDNA contains a single 2,862 bp open reading frame (ORF) which encodes a putative H -ATPase protein of 953 amino acid residues, with a significant homology to plasma membrane H -ATPase of Primus persica, Phaseolus vulgaris, Sesbania rostrata and Daucus carota. The predicted protein has a molecular weight of 104,553 Da. The copy number analysis revealed multiple copies of the PM H -ATPase in the P. euphratica genome after digestion of their genomic DNA by the restriction enzymes EcoRI, NdeⅠ, FbaⅠand BglⅡ, and Southern blot.  相似文献   

EFFECT OF HIGH LOADS IN THE BRIDGE LOAD SPECTRUM ON FATIGUE CRACK GROWTH OF 16 Mn STEEL     

Zhong An  Shen Xianrong 《保鲜与加工》1995,(2):15-22

This paper studies an effect of high loads in Rayleigh distribution random load-ing on fatigue crack growth wi th 16 Mn steel compact tenion specimen,The results show that thehigh loads in the load spectrum close to service load of steel bridges accelerate crack growth and re-duce crack growth life,Lives predicted with constant amplitude da/dn data and equivalent loads arequite in agreement with test lives.  相似文献   

ELLIPSOMETRIC SPECTROSCOPY OF ELECTROCHROMISM OF POLYANILINE FILMS     

Xie Shangfen Shen Hui 《保鲜与加工》1991,(4):93-96

The electrochromism of polyaniline films, which is prepared by electropolymer-ization from aniline in acidic aqueous solution onto platinum,is investigated in 330-700nm through in-situ ellipsometric technique. The results indicate that the ellipsometric peak of the reduced state(-0. 2V) of the film appears at 390-400nm and the oxidized state(0. 55V) at 500-530nm. The elec-trochromic reaction from the reduced "yellow" state to the oxidized "green" state is suggested via intermediate state whose ellipsometric peak appears at 466-470nm.  相似文献   

Complete Genome Sequencing and Analysis of Rehmannia Mosaic Virus Isolate from Shanxi Province     

Wang De-fu  Zhang Xi-mei  Guo Shang  Shen Shao-fei  Long Dan-dan  Li Ling-yu  Niu Yan-bing 《东北农业大学学报(英文版)》2021,28(3):58-65

Using double-stranded RNA(dsRNA) technology and sequence-independent amplification(SIA), the molecular identification on infected Rehmannia glutinosa in the field with mosaic symptoms was performed and the whole-genome of the Rehmannia mosaic virus(ReMV) Shanxi isolate(ReMV-SX) was sequenced. Sequencing analysis showed that the virus that infected Rehmannia glutinosa was Rehmannia mosaic virus(ReMV). The full-length of the obtained ReMV-SX sequence(GenBank accession no. JX575184) was 6 395 nt, containing four open reading frames(ORFs). The sequence homology analysis of the complete nucleotide sequence showed that ReMV-SX was 93.8%-97.0% homologous to ReMV in Tobamovirus subgroup I, while only 49.8%-58.9% homologous to the isolates in subgroups II and III of the same genus. Phylogenetic analysis showed that ReMV-SX and ReMV-Henan formed a separate branch and had the closest genetic relationship. The results laid the foundation for ongoing researches in the taxonomic status and evolution of ReMV and for further investigating the pathogenic mechanism of ReMV infecting Rehmannia glutinosa.  相似文献   

Transcript expression profiles of <Emphasis Type="Italic">Takifugu rubripes</Emphasis> spermatozoa and eggs by expressed sequence tag analysis     

Shen XY  Cui JZ  Gong QL  Liu YJ  Nagahama Y 《Fish physiology and biochemistry》2008,34(3):235-243

  相似文献   

Application of quantum dot-antibody conjugates for detection of sulfamethazine residue in chicken muscle tissue     

Ding S  Chen J  Jiang H  He J  Shi W  Zhao W  Shen J 《Journal of agricultural and food chemistry》2006,54(17):6139-6142

A new indirect competitive fluorescence-linked immunosorbent assay (cFLISA) method for the detection of sulfamethazine (SM2) in chicken muscle tissue was demonstrated using quantum dots (QDs) as the fluorescence label coupled with secondary antibody. The sensitivity of the cFLISA was compared with that of the HPLC method. The 50% inhibition value (IC50) and the limit of detection (LOD) of the cFLISA were 7.7 and 1.0 ng/mL, respectively. When SM2 was spiked at levels of 50, 100, and 200 ng/g, recoveries ranged from 80.6% to 117.4%, with a coefficient of variation of 6.9-9.6%. In the incurred sample analysis, the amounts of SM2 quantified by cFLISA were similar to the results obtained by the HPLC method. This study shows that cFLISA could be used as a screening method in practice.  相似文献   

Identification of two glycerophosphodiester phosphodiesterase genes in maize leaf phosphorus remobilization     

Jingxin Wang  Wenbo Pan  Alexiy Nikiforov  William King  Wanting Hong  Weiwei Li  Yang Han  Jana Patton-Vogt  Jianbo Shen  Lingyun Cheng 《作物学报(英文版)》2021,(1):95-108

  相似文献   

2种规格斜带髭鲷形态性状对体质量影响的相关分析     

萧云朴  吴加将  杨传爱  杨志杰 《现代农业科技》2013,(1):242-244,248

2008年12月至2009年12月期间,随机采集南麂海区养殖的斜带髭鲷(Hapalogenys nitens)1龄鱼211尾,3龄鱼114尾,以体质量(y,g)为因变量,体长(x1,cm)、体高(x2,cm)及尾长(x3,cm)等形态性状为自变量,计算相关系数、通径系数、决定系数及身体状况指数,研究了该鱼体质量与形态性状的内在关系。结果表明:形态性状与体质量的相关系数均达极显著水平(P<0.01),对1龄与3龄鱼体质量的决定系数分别为体长>体高>尾长和体高>体长>尾长,多元线性回归方程分别为:y=-86.259 1+9.370 1x1+6.388 9x2+2.182 6x3(R2=0.904 4**)和y=-652.440 6+17.085 8x1+58.239 8x2+22.089 1x3(R2=0.820 9**)。优度拟合显示:1龄与3龄鱼身体状况指数最佳表示分别为CFJ=M/(HL2)和CFR=M/(H2L)。该研究为斜带髭鲷形态学与选育研究提供参考依据。  相似文献   

Applications and prospects of functional oligosaccharides in pig nutrition: A review     

Nian Liu  Haibo Shen  Fan Zhang  Xiang Liu  Qinrui Xiao  Qian Jiang  Bie Tan  Xiaokang Ma 《动物营养（英文）》2023,(2):206-215

Oligosaccharides are low molecular weight carbohydrates between monosaccharides and polysaccharides, which consist of 2 to 20 monosaccharides linked by glycosidic bonds. They have the effects of promoting growth, regulating immunity, improving the structure of intestinal flora, and are antiinflammatory and antioxidant. With the comprehensive implementation of the antibiotic prohibition policy in China, oligosaccharides as new green feed additive have been paid more attention. Oligosaccharides ca...  相似文献