Reproductive cycle and growth of the sand dollar Scaphechinus griseus inhabiting Japanese surf clam Pseudocardium sachalinense fishing grounds in Tomakomai,southwest Hokkaido,Japan     

Sakurai  Izumi  Abe  Honami  Ogata  Takashi 《Fisheries Science》2019,85(3):465-474

Fisheries Science - Bioturbation by the sand dollar Scaphechinus griseus is suspected to inhibit the recruitment of Pseudocardium sachalinense, a commercially important bivalve. This study aimed to...  相似文献   

Recovery of brodifacoum in vomitus following induction of emesis in dogs that had ingested rodenticide bait     

KH Parton  EK Willson  MG Collett  LH Booth 《New Zealand veterinary journal》2018,66(1):41-43

AIM: To assess the benefit of inducing emesis in dogs that have ingested rodenticide bait containing brodifacoum (BDF), by determining the amount of BDF in bait recovered from the vomitus relative to the estimated amount consumed.

METHODS: Between 2014 and 2015 samples of vomitus from seven dogs that ingested rodenticide baits containing BDF were submitted by veterinarians in New Zealand. All seven dogs had been given apomorphine by the veterinarian and vomited within 1 hour of ingesting the bait. Some or all of the bait particles were retrieved from each sample and were analysed for concentrations of BDF using HPLC. Based on estimations of the mass of bait consumed, the concentration of BDF stated on the product label, and the estimated mass of bait in the vomitus of each dog, the amount of BDF in the vomited bait was calculated as a percentage of the amount ingested.

RESULTS: For five dogs an estimation of the mass of bait ingested was provided by the submitting veterinarian. For these dogs the estimated percentage of BDF in the bait retrieved from the vomitus was between 10–77%. All dogs were well after discharge but only one dog returned for further testing. This dog had a normal prothrombin time 3 days after ingestion.

CONCLUSIONS AND CLINICAL RELEVANCE: The induction of emesis within 1 hour of ingestion can be a useful tool in reducing the exposure of dogs to a toxic dose of BDF. The BDF was not fully absorbed within 1 hour of ingestion suggesting that the early induction of emesis can remove bait containing BDF before it can be fully absorbed.  相似文献   

Differences in lectin-binding properties between the common mucosal epithelium and follicle-associated epithelium in the rabbit small intestine     

Takeuchi T  Fujibuchi T  Imai Y  Kamei Y  Murase R  Kito K  Abe Y  Ueda N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):645-647

Differences in sugar distribution between the villous epithelium and follicle-associated epithelium (FAE) were compared using lectins in the rabbit small intestine. In every portion, villous columnar epithelial cells primarily exhibited a positive reaction to the GalNAc, GlcNAc, galactose, and oligosaccharide. In the ileal Peyer's patch (PP), whereas microvillous epithelial cells exhibited positive reactions, M cells tended to be negative. The villous epithelial reaction to the fucose group was negative, but M cells and microvillous epithelial cells showed a positive to the fucose. No epithelium had a positive reaction to the mannose and glucose. The variety of lectin-binding properties of villous epithelial cells and M cells may reflect specificity for the recognizing luminal substances such as antigenic molecules and bacterial elements.  相似文献   

Role of the hyaluronan receptor CD44 during porcine oocyte maturation     

Yokoo M  Shimizu T  Kimura N  Tunjung WA  Matsumoto H  Abe H  Sasada H  Rodriguez-Martinez H  Sato E 《The Journal of reproduction and development》2007,53(2):263-270

Previous our studies have shown that CD44, the principal receptor for hyaluronan, is present on cumulus cells during oocyte maturation. Although hyaluronan-CD44 interaction has been implicated in cumulus expansion and/or oocyte maturation, the full significance of CD44 remains unknown. The objective of the present study was to further investigate the role of CD44 in cumulus expansion and oocyte maturation in pigs. We demonstrate here in that CD44 has a key role in oocyte maturation but not in cumulus expansion. Previous studies have reported the physiological significance of cumulus expansion in oocyte maturation. However, our results suggest that cumulus expansion is a necessary condition for oocyte maturation, but that it is not sufficient on its own. Furthermore, western blot analysis demonstrated that the CD44 of the in vitro-matured cumulus-oocyte complexes (COCs) had a larger molecular weight and more terminal sialic acid, which has been proven to inhibit the hyaluronan-binding ability of the receptor, than the CD44 of the in vivo-matured COCs, indicating that the hyaluronan-CD44 interactions during in vitro maturation might be insufficient compared with those in vivo. The insufficient interactions of hyaluronan-CD44 during in vitro maturation may cause the inferior capacity of fertilization and development of oocytes matured in vitro.  相似文献   

Molecular dynamics of heterochromatin protein 1beta, HP1beta, during mouse preimplantation development     

Yamazaki T  Kobayakawa S  Yamagata K  Abe K  Baba T 《The Journal of reproduction and development》2007,53(5):1035-1041

To elucidate the molecular dynamics of HP1beta in mouse preimplantation embryos, we examined the localization, dynamics, and mobility of HP1beta in the (pro)nucleus by live cell imaging. Time-lapse observation revealed that the chromatin association of HP1beta is regulated in a cell cycle-dependent manner. HP1beta was localized in the interphase nucleus and was dynamically dissociated from the nucleus during the metaphase stage. The HP1beta assembly and clustered heterochromatin structure were both found in the nuclei of 2-cell and later-stage embryos. Moreover, fluorescent recovery after photobleaching analysis implied that HP1beta is more freely mobile in the pronucleus of the 1-cell embryo than in the 4-cell nucleus. These results suggest that the chromatin configuration may be regulated by the stability and mobility of chromatin-associated proteins including HP1beta during early embryonic stages.  相似文献   

Effect of calcium ion on the thermal denaturation of subfragment-1 and rod regions of squid myosin upon the heating of myofibrils     

Kazuyo Abe  Yasunori Kinoshita  Kunihiko Konno 《Fisheries Science》2012,78(1):163-168

Thermal inactivation of Ca²⁺ ATPase of squid myofibrils was significantly suppressed in the presence of Ca²⁺. Monomeric myosin content decreased much faster than Ca²⁺ ATPase inactivation in Ca medium, which was well explained by fast rod denaturation. In contrast, rod denaturation was slower than S-1 in EDTA medium. The decrease in monomeric myosin content was explained by faster S-1 denaturation. Comparing the S-1 and rod denaturation rates at a fixed temperature, it was concluded that S-1 denaturation was suppressed by Ca²⁺ whereas the rod denaturation was not. An unfolding experiment with isolated myosin rod confirmed that there was no stabilizing effect of Ca²⁺ on myosin rod. It was concluded that significant stabilization of the S-1 portion by Ca²⁺ generated the apparently different myosin denaturation patterns in the two media.  相似文献   

Beneficial effects on meat quality of yellowtail Seriola quinqueradiata induced by diets containing red pepper     

Itaru Shioya  Koji Inoue  Akihisa Abe  Akira Takeshita  Takahiro Yamaguchi 《Fisheries Science》2011,77(5):883-889

The meat quality of farmed yellowtail Seriola quinqueradiata fed on extruded pellets (EP) containing 0.5% (v/v) red pepper (experimental group) was compared with yellowtail of the same age fed on EP (control group). In 1-year-old yellowtail, the crude lipid content of the dorsal muscle of the experimental group tended to be lower than that of the control group. In contrast, there was no difference in the lipid content of the dorsal muscle between the control group and the experimental group in 2-year-old yellowtail. The muscle texture of the experimental group was significantly firmer than that of the control group, with the effect of red pepper unrelated to fish age and lipid content. Color change of red muscle of the experimental group was significantly lower than that of the control group, and the content of thiobarbituric acid-reactive substances in the red muscle was significantly lower in the experimental group than in the control group. These results are the first to demonstrate that the inclusion of red pepper in the diet is able to reduce the loss of muscle texture firmness and to slow down color change in red muscle of yellowtail.  相似文献   

Development of an in vitro culture system for producing eel larvae from immature ovarian follicles in Japanese eel <Emphasis Type="Italic">Anguilla japonica</Emphasis>     

Tomoki Abe  Shigeho Ijiri  Shinji Adachi  Kohei Yamauchi 《Fisheries Science》2010,76(2):257-265

To standardize conditions during the final maturation and ovulation of ovarian follicles from Japanese eel, we have developed a culture system for the production of fertilizable eggs from post-vitellogenic ovarian follicles in vitro. Post-vitellogenic ovarian follicles were incubated in culture medium supplemented with 17α,20β-dihydroxy-4-pregnen-3-one (DHP) with or without bovine serum albumin (BSA) to assess the effects of protein concentration. Eggs that ovulated during incubation were fertilized, and the remaining follicles were incubated in prostaglandin F_2α (PGF_2α) for a further 3 or 6 h before fertilization. Male eels were injected repeatedly with human chorionic gonadotropin. The quality of eggs obtained under the different culture conditions was evaluated after artificial fertilization in terms of hatching success. Hatching rates tended to decrease with increasing concentrations of BSA in the incubation medium in a dose-dependent manner. The addition of PGF_2α drastically increased the number of eggs that ovulated, but the rate of hatching was greatly decreased compared with eggs obtained earlier by DHP incubation alone. The larvae obtained from artificially fertilized eggs produced in vitro survived for 14 days without feeding. We conclude that in vitro culture systems thus have a great potential for the acquisition of good quality eggs under tightly controlled artificial conditions, culminating in the production of eel larvae.  相似文献   

Possible influence of termites (Macrotermes bellicosus) on forms and composition of free sesquioxides in tropical soils     

Susumu S. Abe  Toshiyuki Wakatsuki 《Pedobiologia》2010,53(5):301-306

There has been less concern about soil mineralogical alteration than about soil physical, chemical and biological changes induced by termite nest-building activity. Furthermore, much less attention has been paid to free sesquioxides than to phyllosilicate minerals. In the present study, we conducted field morphological observations and selective dissolution analysis to characterize free sesquioxides in termite (Macrotermes bellicosus) mounds as compared with surrounding pedons in different toposequence positions, i.e., seasonally flooded valley bottom, hydromorphic fringe and well-drained upland sites. Distinctive redoximorphic features, such as surface yellowish layers on mound structures from the fringe site, indicate possible alteration of iron sesquioxide forms in the mounds due to the transportation of soil from reductive (aquic subsoil) to oxidative (epigeal mound) environments by the nest-building activity of M. bellicosus. On the other hand, the iron-soluble content in the dithionite-citrate-bicarbonate (DCB) system (Fe_d) was generally higher in the mound structures than at the adjacent sub-surface (Ap2) horizon at each toposequence position, while there was less difference in the content of acid ammonium oxalate (AAO) extractable iron (Fe_o) as compared to Fe_d. As a consequence, the iron activity index (Fe_d/Fe_o ratio) was found for the most part to be lower in the mound structures than in the neighboring Ap2 horizon. In addition, the content of Fe_d, AAO-soluble Al (Al_o) and DCB-extractable Al (Al_d) was significantly correlated with clay content in these soils. These findings suggest that M. bellicosus preferentially collects clay particles, probably from the clay-rich subsoils, such as the argillic horizon, which has been formed by the co-migration of phyllosilicate minerals and relatively crystalline sesquioxides. The species then likely incorporates them into the mounds, which induces an increase in the Fe_d content relative to that of Fe_o, resulting in a decreased iron activity index in the mound structures.  相似文献   

Veränderungen der Vegetation durch experimentelle Erdgasbehandlung     

PETR PY&#;EK  ANTONÍN PY&#;EK 《Weed Research》1989,29(3):193-204

Changes in vegetation caused by experimental leakage of natural gas The response of vegetation to natural gas leakage was studied in Central Bohemia, Czechoslovakia, in 1978–84. The gas used was of the following chemical composition: 93·8% methane, 3·6% ethane, 0·8% propane, 0·25% butane, 0·3% carbon dioxide. The test crops were planted in 60·5 m plots containing underground bores from which gas was released into the soil through distribution pipes. In addition, the response of some weed species was recorded. The important effects of contamination observed were restricted growth and decrease in the number of individuals present. A change in the green colour of leaves was recorded in Medicago sativa, Brassica oleracea var. acephala, Secale cereale, Lolium multiflorum. Beta vulgaris and Zea mays. An obvious shift of developmental stages was found in Solanum tuberosum. Helianthus annuus and Zea mays (delay of recruitment and blooming). Restricted or even failure of reproduction can be considered the typical effect of exposure to natural gas: this holds true especially for Allium cepa, Triticum aestivum, Hordeum distichon, Trifolium sp.div., Medicago sativa, and most of the weedy species. Various growth deformations, mostly found in the underground parts of the plants (tubers and roots in Solanum tuberosum, Beta vulgaris), can be ascribed to the unfavourable conditions of soil aeration. Plants of Chenopodium album and Medicago sativa growing in the vicinity of the gas release point had an increased number of stomata. There were changes in the course of the reflectance curves estimated in the population of Trifolium pratense subsp. sativum. There was a conspicuous decrease of the near infrared reflectance in the stand exposed to gas in comparison with the control. Decrease of coverage and density of the stands reduced species diversity and changes in the species' composition (reduction of sensitive species) are the general consequences of natural gas leakage. The first symptoms on the vegetation were observed after 15–30 days which corresponds to about 80–150 m³ of discharged gas.  相似文献