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SUMMARY Twenty-four of 57 calves fed a diet containing 33% cotton seed meal (CSM) died between 7 and 15 weeks of age. Initial deaths were not accompanied by premonitory signs, but after CSM withdrawal most calves developed rough coats, anorexia, weakness, ascites and subcutaneous oedema. Those that died had large volumes of serous fluid in the body cavities, hard livers of ‘nutmeg’ appearance, and pulmonary congestion. Histopathologically the livers showed periacinar necrosis in acute cases and periacinar fibrosis in chronic cases. Lungs from several calves had oedema, haemosiderosis and fibrosis in some pulmonary vessels. Atrophy of myocardial fibres was present in most cases. The concentration of free gossypol in the diet was 100 to 220 mg/kg. Ante-mortem and post-mortem findings supported a diagnosis of gossypol poisoning. The deaths continued for 4 weeks after withdrawal of CSM from the diet.  相似文献   
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Different stunning methods (carbon dioxide [CO2] and hypothermia) used in industrial fish processing were compared with asphyxia in air, which is used in traditional fishing, to evaluate the effects of these methods on the stress responses and the meat quality of the Amazon hybrid surubim, Pseudoplatystoma fasciatum female × Leiarius marmoratus male. After the application, blood and behavioral indicators were evaluated. Following death by gill cutting, the fish were gutted, and analyses of muscle pH, rigor mortis, the exudation of the meat, and coloration were performed. Significantly higher levels of cortisol and glucose were exhibited by the fish subjected to asphyxia than by the fish stunned by either CO2 or hypothermia. The fish subjected to asphyxia displayed decreasing muscle pH during the first 2 h after death and showed higher rates of rigor mortis after 3 h than the fish that were stunned by the other two methods. The asphyxia is a practice that exposes fish to suffering by causing an increase in stress responses, which affects meat quality. The immersion of fish in water and ice (hypothermia) for 5 min was more effective stunning method than the use of CO2, resulting in higher loss of sensibility and greater welfare.  相似文献   
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Effect of floor cooling on performance of lactating sows during summer   总被引:2,自引:0,他引:2  
Forty Landrace × Large White lactating sows were used to evaluate the effects of cooling of the floor when maintained under high temperature conditions during summer on their productive and reproductive performance. The sows were allocated in a completely randomized design with two treatments with 20 replicates according to parity number and body weight, with each animal being considered an experimental unit. The treatments consisted of cooling of the floor under the sow with water circulation at about 17 °C and no cooling. The resulting temperatures of the floor were 27.6 and 35.8 °C for the cooled and the control treatments, respectively. The sows from both treatments were exposed to average maximum and minimum environmental temperatures of 26.9 and 20.8 °C, respectively. Sows maintained on a cooled floor had a higher feed intake (6.47 vs. 5.61 kg/day; P < 0.01). Despite this higher intake, sows maintained on a cooled floor had higher body weight and body protein losses during the lactation period (P < 0.01) in connection with a higher milk yield and subsequent growth of the litter (2280 vs. 1798 g/day; P < 0.01). There was an effect of treatment on rectal temperature, surface temperatures and respiratory rate (P < 0.01) with lower values in sows submitted to floor cooling. It is concluded that floor cooling under the lactating sow improves its productive and reproductive performance, as well as the performance of its litter.  相似文献   
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Pinguinain is the name given to a proteolytic enzyme preparation obtained from Bromelia pinguin fruits that has been scarcely studied. The present paper deals on the reexamination of the proteases present in fruits of B. pinguin grown in Cienfuegos, Cuba. The preparation (partially purified pinguinain, PPP) showed the main characteristics of the cysteine proteases, i.e., optimum pH within alkaline range (pH 7.2-8.8), inhibition of proteolytic activity by thiol blocking reagents, which is usually reverted by addition of cysteine, a remarkable thermal stability and notable stability at high ionic strength values. Isoelectric focusing and zymogram of PPP revealed the presence of several proteolytic components between pI 4.6 and 8.1. Preliminary peptidase purification by cationic exchange chromatography showed the presence of two main proteolytic fractions with molecular masses of approximately 20.0 kDa, according to SDS-PAGE.  相似文献   
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Journal of Soils and Sediments - This study consists of the evaluation of metals acute toxicity levels taking into consideration a hypothetic scenario of dam breaking and land deposition of two...  相似文献   
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Chlamydiosis in workers at a duck farm and processing plant   总被引:4,自引:0,他引:4  
SUMMARY An outbreak of chlamydiosis was Investigated in workers at a duck farm and processing plant during winter 1989. Chlamydia psittaci was isolated from ducks, but there was little evidence of clinical chlamydiosis in the ducks. Serological tests showed that 76% of workers had been exposed to Infection, 12% recently, 16% had not been exposed and 8% had inconclusive test results. There was a strong association (P < 0.05) between years of employment and exposure to Infection. Infection appeared to be contracted during the first year of employment. Measures were Implemented to reduce shed contamination and control rodents and wild birds. At the same time, ducks were medicated, the processing plant was modified, work practices were altered and workers were advised about chlamydiosis to reduce the risk of human infection.  相似文献   
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