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为研究不同浓度的赤霉酸对饲料添加菌生长的影响,用2种不同浓度的赤霉酸溶液单独添加饲料乳酸菌(A4+A7)和纤维素分解菌(Nf+Y6)进行培养52 h,其中每4 h作一个单位测定出OD600 nm值并绘制生长曲线,分析不同浓度的赤霉酸对饲料添加菌生长的影响。结果表明,赤霉酸浓度为10 mg/L时,各组OD600 nm值分别为0.64、0.70、0.84、0.78、0.72,其中试验组2的OD600 nm值与对照组和其他试验组相比有明显增高,总活菌数高达11.6×108 CFU/mL,比对照组(1.63×108 CFU/mL)高7倍以上;当赤霉酸浓度增加到20 mg/L时,各组OD600 nm值分别为0.64、0.60、0.59、0.59、0.63,其中各试验组的OD600 nm值与对照组相比无明显差异(P>0.05),试验组活菌数(1.60×108 CFU/mL)与对照组相比(1.63×108 CFU/mL)无明显差异(P>0.05)。通过试验数据和生长曲线得知赤霉酸浓度在10 mg/L时能促进乳酸菌和纤维素分解菌的生长繁殖;赤霉酸浓度为20 mg/L时乳酸菌和纤维素分解菌的生长速度明显下降。综上提示,适当添加赤霉酸对饲料添加菌生长有明显的促进作用,赤霉酸浓度过高则饲料添加菌的生长量降低。  相似文献   
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试验旨在研究氨基酸副产物(amino acid by-products,ABP)对全株红高粱和全株甜玉米发酵品质及消化率的影响,并通过扫描电镜(SEM)探讨ABP对青贮饲料发酵品质及消化率的作用机制。试验设无添加剂的对照组和添加2.0% ABP的试验组Ⅰ、2.0% ABP与饲用菌混合添加的试验组Ⅱ,分别进行红高粱和甜玉米的发酵试验,检测其发酵品质及消化率。结果表明:①试验组Ⅰ红高粱和甜玉米青贮pH降至3.90和3.28,感官评定均属于优质青贮饲料。②对于红高粱青贮,与对照组相比,试验组干物质(DM)含量略有提高,但差异不显著(P>0.05),粗蛋白质(CP)含量显著高于对照组(P<0.05),试验组Ⅱ纤维成分略低于对照组,但差异不显著(P>0.05);各试验组乳酸和乙酸含量显著高于对照组(P<0.05),试验组Ⅱ丁酸含量显著低于其余组(P<0.05);试验组干物质和中性洗涤纤维(NDF)消化率均高于对照组,但差异不显著(P>0.05),酸性洗涤纤维(ADF)消化率显著高于对照组(P<0.05)。③对于甜玉米青贮,各试验组干物质和纤维含量均低于对照组,但差异不显著(P>0.05),粗蛋白质含量显著高于对照组(P<0.05);试验组Ⅰ乳酸含量显著高于其余组(P<0.05),试验组Ⅱ乙酸含量显著高于其余组(P<0.05),而各试验组丁酸含量显著高于对照组(P<0.05);试验组各养分消化率均低于对照组但高于未发酵原料,其中干物质和酸性洗涤纤维消化率差异显著(P<0.05)。④SEM结果表明,青贮过程中ABP通过破坏红高粱表面蜡质层促进饲用菌的黏附,并降解其细胞壁纤维成分而改善了青贮发酵品质并提高消化率;但ABP对青贮甜玉米蜡质层及细胞壁纤维结构破坏作用不明显。综上,添加ABP可提高红高粱青贮的发酵品质和养分消化率,但对甜玉米青贮的作用不大。  相似文献   
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采用分光光度计和SPAD仪分别测定35个海岛棉品种盛花期功能叶叶绿素各成分含量(叶绿素a、b和类胡萝卜素含量分别表示为Ca、Cb、Cxc)、总叶绿素含量(CT)和SPAD值,并进行比较,分析其关系和变化规律。结果显示:(1)35个海岛棉品种开花盛期叶片SPAD值间与叶绿素含量各指标间有极显著差异, 新海3号的Ca、Cb、CT最高,新海18号的为最低;新海8号SPAD值最高,新海41号SPAD值最低;新海31号的Cxc含量最高,新海18号最低。(2)海岛棉功能叶片的SPAD值与Ca、Cb和CT之间具有显著的正相关, Ca、Cb与CT间极显著相关,相关系数分别为0.931、0.990及0.972,Cxc与其他指标均无显著相关。(3)依据SPAD值与叶绿素含量各指标进行聚类,35个海岛棉品种分为5类,新海8号Ca、Cb、CT及SPAD值最高。  相似文献   
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This study was conducted to investigate the effects of amino acid by-products(ABP) on fermentation quality and digestibility of red sorghum and sweet corn,and explore the mechanism of action by scanning electron microscopy(SEM).There was a control group without additives,an experimental group Ⅰ with 2.0% ABP,and an experimental group Ⅱ with 2.0% ABP mixed with forage fungus for red sorghum and sweet corn,respectively.The results showed that:①2.0% ABP reduced the pH of red sorghum and sweet corn silage to 3.90 and 3.28 respectively,and they were belonged to the high-quality silage range by sensory evaluations.②For the red sorghum silage,compared with the control group,the dry matter (DM) contents of the experimental groups were slightly increased,but the difference were not significant (P>0.05),while the crude protein (CP) contents were significantly increased (P<0.05).The fiber content of the experimental group Ⅱ was slightly lower than the control group,but the difference was not significant (P>0.05).The lactic acid and acetic acid contents of experimental groups were significantly higher than that of control groups (P<0.05).The content of butyric acid in experimental group Ⅱ was significantly lower than that of the other groups (P<0.05).The dry matter and neutral washing fiber (NDF) digestibilities of the experimental groups were higher than the control group,but the difference were not significantly (P>0.05),and acidic washing fiber(ADF) digestibility was significantly higher than control group (P<0.05).③ For sweet corn silage,the dry matter and fiber contents of experimental groups were lower than those in the control group,but the differences were not significant (P>0.05),the crude protein content was significantly higher than the control group (P<0.05).The lactic acid content of the experimental group Ⅰ was significantly higher than the other groups (P<0.05),the acetic acid content of experimental group Ⅱ was significantly higher than that of the other groups (P<0.05),and the butyric acid content of each experimental group was significantly higher than the control group (P<0.05).The digestibilities of the experimental groups were lower than the control group but higher than the raw materials,and the differences of dry matter and acid washing fiber digestibilities were significant (P<0.05).④SEM results showed that ABP promoted the adhesion of forage bacteria by destroying the waxy layer on the surface of red sorghum silage,and degraded the cell wall fiber components to improve the quality of the silage fermentation and improve the digestibility during the silage process.But the destructive effect of ABP on sweet corn silage was not obvious.In conclusion,the addition of ABP could improve the fermentation quality and digestibility of red sorghum,but the effect on sweet corn silage was not obvious.  相似文献   
5.
采用单因素实验和正交实验方法,以感官评定及最终pH值为评价标准,对保加利亚乳杆菌(Lactobacillus bulgaricum)和嗜热链球菌(Streptococcus thermophilus)发酵鹰嘴豆乳与牛乳混合乳的最佳条件进行了研究。结果表明,当豆水比为1∶8(kg∶L),牛乳与豆乳混合比为1∶2(L∶L),接种量为3%,发酵温度为39℃,发酵时间为24h时,产品的豆腥味较淡,酸味、口感最好。  相似文献   
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在大田生产条件下,对6个转基因抗虫杂交棉品种光舍性能及产量和品质性状进行了比较研究,结果表明,与豫杂35相比,中杂7号的叶片SPAD值、光舍性能和Fv/Fm,Fv/Fo,ФPS Ⅱ在部分时间下降,产量无显著差异,纤维品质指标有优有劣;中棉38叶片SPAD值、光合性能和Fv/Fm,Fv/Fo,ФPSⅡ总体较低,产量减少,但纤维品质较好;中棉48叶片光舍性能优于豫杂35,结铃数极显著减少,但铃重极显著增加,产量无显著差异,纤维品质较优;泗杂3号叶片SPAD值、光舍性能和Fv/Fm,Fv/Fo,ФPSⅡ有升有降,增产达极显著水平,但纤维品质略差;郑杂1号叶片Fv/Fm,Fv/Fo,ФPSⅡ总体较低,皮棉减产,品质也略差。  相似文献   
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