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  1. The apparent (AIAAD) and standardised ileal amino acid digestibility (SIAAD) of wheat distillers dried grains with solubles (wheat-DDGS) was determined for broilers and turkeys at 28 d using two experiments.

  2. A total of 84 male Ross 308 broiler chicks (Experiment 1) or 84 male BUT 10 turkey poults (Experiment 2) were offered a nutrient-adequate diet from d 1 to 23. On d 23, birds in each experiment were allocated to 4 treatments consisting of a nitrogen-free diet (NFD) (with or without protease) and an assay diet containing wheat-DDGS as the only source of amino acids (with or without protease) in a randomised complete block design.

  3. In Experiments 1 and 2, the coefficient of AIAAD or SIAAD of Lys and Asp were low irrespective of protease. In Experiment 1, the coefficient of AIAAD ranged from 0.35 (Ala) to 0.75 (Pro) without protease, whereas the range was 0.42 (Thr) to 0.82 (Pro) with protease. Protease improved the coefficient of AIAAD for Arg and Pro and tended to improve the coefficient of AIAAD for Met. Without protease, the coefficient of SIAAD ranged from 0.51 (Ala) to 0.84 (Pro), whereas the range was from 0.65 (Ala) to 0.93 (Pro) with protease. Protease improved the coefficient of SIAAD of Arg, Leu, Phe, Met, Val and Pro.

  4. In Experiment 2, the coefficient of AIAAD was lower than 0.50 for all amino acids except for Glu (0.70) and Pro (0.81) without protease. On the other hand, the coefficient of SIAAD ranged from 0.41 (Thr) to 0.89 (Pro) without protease, whereas the range was from 0.56 (Arg) to 0.88 (Pro) with protease supplementation. With the exception of Cys and Pro, protease improved the coefficient of AIAAD and SIAAD of all other amino acids by 0.05–0.19.

  5. It was concluded that the AIAAD and SIAAD of wheat-DDGS are variable and are generally greater in broilers compared to turkeys at 28 d of age. Protease improved the ileal digestibility of a large number of amino acids in wheat-DDGS for broilers and turkeys.

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The blood pressure lowering effect of a pea protein hydrolysate (PPH) that contained <3 kDa peptides, isolated by membrane ultrafiltration from the thermolysin digest of pea protein isolate (PPI), was examined using different rat models of hypertension as well as hypertensive human subjects. The PPH showed weak in vitro activities against renin and angiotensin converting enzyme (ACE) with inhibitory activities of 17 and 19%, respectively, at 1 mg/mL test concentration. Oral administration of the PPH to spontaneously hypertensive rats (SHR) at doses of 100 and 200 mg/kg body weight led to a lowering of hourly systolic blood pressure (SBP), with a maximum reduction of 19 mmHg at 4 h. In contrast, orally administered unhydrolyzed PPI had no blood pressure reducing effect in SHR, suggesting that thermolysin hydrolysis may have been responsible for releasing bioactive peptides from the native protein. Oral administration of the PPH to the Han:SPRD-cy rat (a model of chronic kidney disease) over an 8-week period led to 29 and 25 mmHg reductions in SBP and diastolic blood pressure, respectively. The PPH-fed rats had lower plasma levels of angiotensin II, the major vasopressor involved in development of hypertension, but there was no effect on plasma activity or renal mRNA levels of ACE. However, renal expression of renin mRNA levels was reduced by approximately 50% in the PPH-fed rats, suggesting that reduced renin may be responsible for the reduced levels of angiotensin II. In a 3-week randomized double blind placebo-controlled crossover human intervention trial (7 volunteers), significant (p<0.05) reductions (over placebo) in SBP of 5 and 6 mmHg were obtained in the second and third weeks, respectively, for the PPH group. Therefore, thermolysin derived bioactive peptides from PPH reduced blood pressure in hypertensive rats and human subjects, likely via effects on the renal angiotensin system.  相似文献   
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Appropriate planting patterns ensure sustained soil fertility and higher productivity for farmers. This study assessed the intercropping crop combinations and information sources of kola farmers in Osun State, Nigeria. Sixty respondents were selected using multistage and purposive random sampling techniques. Data were collected with a structured questionnaire. The arable crops commonly intercropped with kola were plantain/banana, yam, cassava, and maize. Tree crops combined with kola included cocoa, citrus, and oil palm. Co-farmers/friends and radio served as popular sources of information for farmers. Farm size was significantly related to respondents’ information sources on kola intercropping. The authors recommend that the Cocoa Research Institute of Nigeria's extension personnel make greater use of information sources most frequently used by respondents in order to ensure adequate cropping systems.  相似文献   
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Veterinary Research Communications - Infections with divergent strains of astroviruses appear to be endemic in commercial poultry. In order to investigate enteric viruses associated with hatchery...  相似文献   
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In cropping systems with limited amounts of external inputs, the soil organic matter pool (SOM) may contribute significantly to plant nutrition. The impact of organic inputs on total SOM and particulate organic matter (POM) N contents as affected by soil type and the relationships between sources of N and maize N uptake were assessed for a set of alley cropping trials in the West- African moist savanna. The trials were established in Niaouli (Bénin Republic), in Glidji, Amoutchou, and Sarakawa (Togo), and in Bouaké and Ferkessédougou (Côte d‘ Ivoire). The total soil N content, averaged over all treatments and years, varied between 324 and 1140 mg N kg?1 soil. The POM-N content varied between 50 and 160 mg N kg?1 soil. The average proportion of soil N belonging to the POM pool ranged between 9% and 29%. This was significantly related to the annual N inputs from maize stover and prunings, when averaged over the different alley cropping treatments. The trial ‘age‘ also appeared to be related to the impact of the different treatments on the POM-N content. The Ferkessédougou soil contained a relatively higher proportion of total soil N in the POM pool because of its relatively high silt and clay content, compared to the other sites. The relative change in POM-N content between 1996 and the initial sampling was about twice the relative change in total soil N content. This suggests that N incorporated in the POM is relatively labile, compared to N incorporated in the other SOM fractions. Maize N uptake was related to the amount of add pruning-N (partial r2 of 27%), the rainfall during the growing season (partial r2 of 17%), the POM-N content (partial r2 of 14%), and to a lesser degree to the POM N concentration (partial r2 of 5%), the fertilizer N addition rate (partial r2 of 3%), and the silt and clay content of the soil (partial r2 of 3%). The POM-N content was shown to be influenced by organic matter additions and soil characteristics and to contribute significantly to maize N supply. This pool may be an important indicator for the soil fertility status of savanna soils.  相似文献   
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Plasma sex steroid hormonal profile and gonad histology were correlated to study the annual reproductive cycle of Hemibagrus nemurus. Hormones were measured by Enzyme Linked Immunosorbent Assay. Gonad tissues were observed by using light microscopy. The highest testosterone (T) value for male was observed in November and that of female was in October. 11-ketotestosterone (11-KT) and 17β-estradiol (E2) levels were highest in June and November, respectively. Hormonal profiles of T, 11-KT and E2 showed several peaks which indicated a non-seasonal pattern. There were significant differences (p < 0.05) in the monthly levels of T, 11-KT and E2. Gonadosomatic index of H. nemurus ranged from 1.14 ± 0.02 % to 7.06 ± 1.40 %, and high gonadosomatic indices were recorded in May, August and November. Gonad histology revealed that spermatozoa were always present in the testes which implied continuous spermatogenesis and asynchronous ovarian development pattern was observed in the ovaries. The annual reproductive cycle of H. nemurus did not show a seasonal pattern and this indicate that H. nemurus is a non-seasonal breeder with several spawning cycles and can be referred to as indeterminate batch spawner. The major significances of this study are annual sex steroid hormonal profile and asynchronous ovarian development of H. nemurus. This information will contribute to our knowledge of reproductive biology of H. nemurus.  相似文献   
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