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1.
In order to establish a genetic model of the resistance to Phytophthora cupsici in Capsicum annuum genotype‘Line 29′, three experiments were conducted which included, as well as‘Line 29′, the susceptible genotype‘Morron INIA 224’and several of its F1, F2, F3 and backcrosses. Plants with 4–6 leaves were inoculated by irrigating the culture substrate with a zoospore suspension of isolate B 1. The F2 test was applied to the segregating generations to test whether there were one, two, three or four genes involved in the resistance. Additivity and equal weight of all the genes in the final effect were assumed. The hypothesis that best explained the results obtained was the one that assumed three genes in‘Line 29′; at least four alleles had to be present in any genotype for it to behave as resistant. The possible influence of isolate aggressiveness and inoculation method on the results is discussed. 相似文献
2.
Ayala MD López Albors O García Alcázar A Abellán E Latorre R Vázquez JM Ramírez Zarzosa G Martínez F Gil F 《Anatomia, histologia, embryologia》2003,32(5):271-275
Muscle growth was studied in larvae of sea bass, Dicentrarchus labrax L., reared at two temperatures: real ambient temperature ( congruent with 15 degrees C during vitelline phase and increased gradually) and 19 degrees C from fertilization until the end of larval development. Muscle cellularity, body length and body weight were measured. Early temperature influenced larval development and so, pre-larval phase finished earlier at 19 degrees C than at ambient temperature (4 and 6 days, respectively). Temperature also affected muscle growth such that at hatching and at mouth opening hypertrophy of muscle fibres was greater at 19 degrees C (P < 0.05), whereas hyperplasia was similar in both groups. After 25 days, the cross-sectional area of the white muscle was greater at 19 degrees C (P < 0.05), which was mainly associated with a higher proliferation of new white muscle fibres. At this stage the body length was also higher at 19 degrees C. Metamorphosis finished earlier in fish reared at 19 degrees C (52 days) than at natural temperature (82 days). At this developmental stage body length and cross-sectional area of the myotome were similar in both groups. However, muscle cellularity differed between groups. Thus, hypertrophy of muscle fibres was higher in fish reared at ambient temperature (P < 0.05), whereas proliferation of new muscle fibres was higher at 19 degrees C (P > 0.05). 相似文献
3.
Maria Vargas Amparo Chiralt Ana Albors Chelo González-Martínez 《Postharvest Biology and Technology》2009,51(2):263-271
Edible coatings based on high molecular weight chitosan, pure or combined with methylcellulose or oleic acid, were applied to fresh-cut carrots cv. Nantesa by simple immersion and by applying a vacuum pulse (5 kPa for 4 min). Water vapour resistance, colour, mechanical properties and respiration rates of non-coated and coated samples were determined. Coatings improved sample appearance, since they diminished the occurrence of the white blush during storage. When applied by simple immersion, they neither conferred significant barrier properties nor the preservation of the mechanical properties of fresh-cut carrot samples. In contrast, coating application with a vacuum pulse enhanced all the positive effects, since the resistance of water vapour transmission of the samples was significantly improved, and better preservation of the sample colour and mechanical response during cold storage was obtained. Differences in film composition did not significantly affect the coating behaviour, probably due to the variability induced by different factors when coatings were applied to the carrot surface. 相似文献
4.
Rafael Latorre Kees de Jong Mircea‐Constantin Sora Octavio Lpez‐Albors Carlos Baptista 《Anatomia, histologia, embryologia》2019,48(6):557-563
Epoxy plastination techniques were developed to obtain thin transparent body slices with high anatomical detail. This is facilitated because the plastinated tissue is transparent and the topography of the anatomical structures well preserved. For this reason, thin epoxy slices are currently used for research purposes in both macroscopic and microscopic studies. The protocol for the conventional epoxy technique (E12) follows the main steps of plastination—specimen preparation, dehydration, impregnation and curing/casting. Preparation begins with selection of the specimen, followed by freezing and slicing. Either fresh or fixed (embalmed) tissue is suitable for epoxy plastination, while slice thickness is kept between 1.5 and 3 mm. Impregnation mixture is made of epoxy E12 resin plus E1 hardener (100 ppw; 28 ppw). This mixture is reactive and temperature sensitive, and for this reason, total impregnation time under vacuum at room laboratory temperature should not last for more than 20–24 hr. Casting of impregnated slices is done in either flat chambers or by the so‐called sandwich method in either fresh mixture or the one used for impregnation. Curing is completed at 40°C to allow a complete polymerization of the epoxy‐mixture. After curing, slices can be photographed, scanned or used for anatomical study under screen negatoscope, magnification glass or fluorescent microscope. Based on epoxy sheet plastination, many anatomical papers have recent observations of and/or clarification of anatomical concepts in different areas of medical expertice. 相似文献
5.
Gómez A Del Palacio JF Latorre R Henry RW Sarriá R Albors OL 《Veterinary radiology & ultrasound》2012,53(2):197-203
Our aim was to compare plastinated sections of the canine heart with corresponding two-dimensional (2D) echocardiographic images. Thirteen dog hearts were fixed by dilation and then processed by the S10 silicon plastination method (Biodur). Two dogs without evidence of cardiac disease were imaged using 2D echocardiography so as to obtain a complete series of the standard right and left parasternal images, which were compared with corresponding plastinated slices obtained by knife sectioning of the hearts. The plastinated slices revealed the internal anatomy of the heart with great detail and were particularly useful to display the spatial relationship between complex anatomic structures. The plastinated slices corresponded accurately with the echocardiographic images. Because of the dilation of the right heart during the fixation process, it was not possible to obtain plastinated specimens in ventricular systole. This paper may be a reference atlas for assisting 2D echocardiography interpretation. 相似文献
6.
Ibabe Martínez I Gil Cano F Ramírez Zarzosa G Vázquez JM Latorre R López Albors O Arencibia A Orenes YM 《Anatomia, histologia, embryologia》2000,29(4):211-219
Cross-sections of the lateral musculature of 19 species of teleost fish of the Percomorphi order were studied histochemically and morphometrically. The red, pink and white muscles were observed in all the species except in Seriola dumerilii and Scomber japonicus, in which no pink muscle was found. The red fibres were the smallest due to their minimal diameters. Histochemically and according to their location at least five types were found in the same species: typical red, in rosette, of the border, infiltrated pink and in mosaic. The pink fibres were larger than the red but smaller than the white. The form and disposition of the pink muscle varied according to the species. This muscle is made up by fibres of medium size, moderate m-ATPase and intermediate oxidative activities. Their histochemical properties change near red and white muscles, where they acquire the histochemical characteristics of the typical red fibres or the low m-ATPase activity of white fibres, respectively. The white muscle is the widest muscular layer. All of the species, except Thalassoma pavo are made up by three fibre types (small with high m-ATPase activity, medium with moderate m-ATPase activity, and large with low m-ATPase activity). They are mixed and show a histochemical and morphometric mosaic form. The extent of this mosaic depends on the species. In most of the analysed species it was observed in areas close to the pink muscle, but in three of them (Liza aurata, Seriola dumerilii and Coris julis) it occupied all of the myotome. 相似文献
7.
Effect of triploidy on muscle cellularity and flesh quality of turbot (Scophthalmus maximus) 
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下载免费PDF全文 Jorge Hernández‐Urcera María Dolores Ayala Marina Santaella María Jesús Periago Octavio López‐Albors Rosa Cal 《Aquaculture Research》2017,48(7):3606-3617
Production of triploid turbot is a relevant goal for the aquaculture industry due to the benefits associated with their sterility and growth. In order for a better understanding of the final quality of the species, muscle cellularity and quality parameters of the flesh were studied in seven diploid and seven triploid turbot after sexual maturation. White muscle cellularity was evaluated throughout white muscle cross‐sectional area from the epaxial left muscle section of each fish. To ascertain the flesh quality, several physico‐chemical parameters (moisture, ash, protein, total fat, fatty acids and minerals) were analysed, and textural mechanical properties (springiness, hardness, chewiness, cohesiveness, gumminess and adhesiveness) were determined objectively with a texturometer. Muscle cellularity was different between both groups, such that muscle fibre density was higher for diploid specimens (P < 0.001) and muscle fibres diameters were higher for triploids (P < 0.001). Both ploidy groups showed no significant differences in moisture, ash, protein and total fat content (P > 0.05). All textural properties analysed showed no significant differences between both ploidy groups (P > 0.05). In both ploidy groups, fibre density and fibre diameter showed no significant correlations (P > 0.05) with textural parameters such as springiness, cohesiveness, chewiness and adhesiveness. Ploidy did not affect fatty acid composition, with the exception of eicosapentaenoic acid (C20:5ω3) which was higher in the triploid group. No significant differences (P > 0.05) existed between diploid and triploid turbot in content of all minerals analysed. According to our results, the ploidy status determines significant variations on muscle cellularity but does not influence the flesh quality of the turbot. 相似文献
8.
F. Tristán M. A. Rivero O. L. Albors G. Ramis J. M. Vázquez M. Martínez J. S. Martínez F. Gil 《Anatomia, histologia, embryologia》2009,38(4):275-278
The objective of this study was to determine the relationship between body weight and the size, number and proportion of muscle fibre types in the pig semitendinosus muscle at birth. Based on weight at birth, 68 specimens selected from 21 different litters from the same farm were allotted in two equal groups (G1 and G2). G1 included 34 piglets ≤1.1 kg and G2 34 pigs ≥2 kg. Fifteen piglets per group were killed at birth and the remaining 19 piglets in each group were reared until weaning (21 days) and post-weaning (67 days). The weight and total cross-sectional area of the semitendinosus muscle were recorded at birth. Then, type I and type II fibres from the superficial portion of the muscle were identified according to histochemistry and immunohistochemistry techniques and percentages, average size of each fibre type, and the total number of muscle fibres were estimated by morphometry. Birth weight in G1 was 54.74% lower than that in G2. Correspondingly, the total cross-sectional area of the semitendinosus, as well as the size and number of muscle fibres, was significantly lower in G1 ( P < 0.001). Weight at birth still influenced weights at weaning and post-weaning, hence it was 43.17% and 28.38% lower respectively in G1. It is concluded that pig weight at birth is associated with muscle cellularity of the semitendinosus muscle of pig, which may influence the postnatal muscle growth and final size of muscle fibres and meat quality. 相似文献
9.
López Albors O Rojo D Sarriá R Soria F Pérez Cuadrado E Latorre R 《Veterinary journal (London, England : 1997)》2011,190(1):113-118
Double balloon endoscopy (DBE) enables the diagnosis and treatment of small intestinal disease. The dog is a potential animal model for DBE training and also a candidate for the clinical application of this technique. However, the anatomy of the canine small intestine may limit the use of DBE by restricting the push-and-pull manoeuvres required for DBE. To establish what these limitations are, the length and width of different portions of the small intestine as well as the mesenteric dependences were measured in 55 dogs. Several external parameters related to body size plus the age, weight, sex and breed were recorded. Potential restriction of the progress of the enteroscope due to narrow intestinal diameter is expected in the jejunum of mid- and small size dogs. 相似文献
10.
R. Sarria O. López Albors F. Soria I. Ayala E. Pérez Cuadrado P. Esteban R. Latorre 《Veterinary journal (London, England : 1997)》2013,195(3):331-336
Exploration of the canine small intestine using conventional endoscopy is restricted to the duodenum and/or the ileum. Double balloon endoscopy (DBE) is a ‘push and pull’ technique that has been described in humans and permits a complete exploration of the small intestine. In this study, oral DBE was performed on 12 healthy dogs (10–34 kg) to characterize for the first time the efficiency, exploration dynamics and safety of the technique.DBE was successful in 83% of dogs; the average estimated insertion depth of the endoscope was 287 ± 36 cm, and the average duration of the exploration was 84 ± 8 min. No complications or relevant adverse clinical effects were observed, and there was no indication of post-procedure pancreatitis based on serology of two specific markers of pancreatitis (amylase and lipase) and the immediate nonspecific inflammatory mediator C-reactive protein. The study showed that oral DBE is viable and safe in the dog, allowing for the diagnosis and treatment of gastrointestinal diseases deep in the small intestine to an extent that has not previously been possible using conventional endoscopy. 相似文献