Naturally occurring estrogens in processed milk and in raw milk (from gestated cows)     

Malekinejad H  Scherpenisse P  Bergwerff AA 《Journal of agricultural and food chemistry》2006,54(26):9785-9791

The occurrence of the steroid hormones estrone (E1), 17alpha-estradiol (alphaE2), 17beta-estradiol (betaE2), and estriol (E3) in processed bovine milk with different fat contents and in raw milk from (non)gestated cows was investigated. Following liquid extraction, optional enzymatical deconjugation, C18 solid-phase extraction, and derivatization, estrogens were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Free and deconjugated E1 (6.2-1266 ng/L) was the major estrogen followed by alphaE2 (7.2-322 ng/L) and betaE2 (5.6-51 ng/L), whereas E3 was detected regularly at the detection limit of 10 ng/L. The lowest and highest concentrations were determined in raw milk from nonpregnant and from cows in the third trimester of gestation, respectively. The estrogen concentration in processed milk coincides with that of raw milk between first and second trimesters, reflecting the contribution of lactating pregnant cows to the final consumable product. The daily intake of total investigated estrogens through milk is 372 ng, which is dramatically more than currently recognized.  相似文献   

Using grounded theory to examine people's attitudes toward how animals are used.     

Sarah Knight  Karl Nunkoosing  Aldert Vrij  Julie Cherryman 《Society and Animals》2003,11(4):307-327

This study uses qualitative methodology to examine why people have different attitudes toward different types of nonhuman animal use. Seventeen participants took part in a semi-structured interview. The study used Grounded Theory to analyze the interviews and developed a model that consists of 4 major themes: (a) "attitudes toward animals," (b) "knowledge of animal use procedures," (c) "perceptions of choice," and (d) "cost-benefit analysis." The findings illustrate that cognitive processing, characteristics of the species of animal being used, and the type of animal use can all influence attitudes toward animal use. Because previous research has focused on participant variables such as age and gender to explain variance in attitudes toward animal use (Furnham & Pinder, 1990; Kellert & Berry, 1981) and measured attitudes toward animal use in general (rather than distinguishing between different types of use) (Armstrong & Hutchins, 1996), these findings can add to knowledge of people's views on animal use. This paper discusses how such views may be justified and maintained.  相似文献   

Ochratoxin a contents in wine: comparison of organically and conventionally produced products     

Chiodini AM  Scherpenisse P  Bergwerff AA 《Journal of agricultural and food chemistry》2006,54(19):7399-7404

Ochratoxin A (OTA) content was determined in 44 organically and conventionally produced wines originating from different geographical regions. Wine samples were extracted using a series of C18 and mixed-bed solid-phase cartridges and analyzed by HPLC with fluorescence detection. The identity of the mycotoxin was confirmed using liquid chromatography-tandem mass spectrometry. Recoveries were in excess of 90%, intraday precisions were better than 6%, and the interday variation was 15%. Limit of detection was 0.05 microg/L (HPLC). All sampled wines contained OTA below the level permitted by the European Union of 2 microg/L, ranging from not detectable (nd) to 0.75 microg/L for red wines (n = 26), from nd to 0.092 microg/L for rosé wines (n = 2), and from nd to 0.22 microg/L for white wines (n = 16). The concentration of OTA in organically produced wines (nd to 0.72 microg/L, median 0.092 microg/L, n = 19) was not significantly different from that in conventional products (nd to 0.75 microg/L, median 0.066 microg/L, n = 25) as assessed by a Mann-Whitney statistical test (p = 0.54).  相似文献