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自1974年发现木豆雄性不育株以来,国际上不断对木豆核雄性不育(GMS)和核质互作雄性不育(CGMS)基因及其种质资源进行研究和挖掘,最终实现了木豆杂种优势的成功利用。本文系统总结了过去30多年来,国际上木豆杂种优势机理、表现及授粉方式等基础研究,GMS、CGMS不育系及其配套制种体系研究,杂种优势强度研究,以及木豆杂交种生产技术研究进展。提出了木豆杂种优势利用的具体研究方向和展望。 相似文献
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Alharbi KB Al-Swailem A Al-Dubaib MA Al-Yamani E Al-Naeem A Shehata M Hashad ME Albusadah KA Mahmoud OM 《Tropical animal health and production》2012,44(1):173-177
Camels are the prime source of meat and milk in many desert regions of the world including Saudi Arabia. Paratuberculosis
of camels, locally called Silag, is a serious and invariably fatal disease in the Arabian camel. Six camels were used in this
study. Five camels with clinical paratuberculosis were used to study the pathology of the disease and confirm its aetiology.
The sixth camel was clinically healthy and used as a control. The camels were examined clinically and bled for haematological
and blood chemistry analysis. They were then humanely killed with a high intravenous dose of thiopental sodium (10 mg/kg)
for pathological studies as well as obtaining tissues for microbiological and molecular studies. The clinical signs of the
disease were emaciation, diarrhoea, alopecia, wry neck and pale mucous membranes. Laboratory diagnosis showed reduced haemoglobin
concentration, low haematocrit and high activity of the serum enzyme alanine aminotransferase. Serum creatinine concentration
was normal. These results indicated the infected camels were anaemic and the function of their livers was affected. Postmortem
examination showed thickened and corrugated intestinal mucosa, enlarged granulomatous mesenteric lymph nodes, miliary and
diffuse granulomas in the liver (in four camels), generalized lymph node granulomas (in one camel), splenic granuloma (in
one camel) and mediastinal lymph node granuloma (in two camels). Histopathological examination showed diffuse infiltration
of macrophages in all organs showing lesions. Ziehl–Neelsen staining of tissue scraping and tissue sections showed masses
of acid fast bacilli, except for the spleen. Infection with Mycobacterium avium subsp. paratuberculosis was confirmed by PCR by targeting the IS900 gene. 相似文献
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ST Rolim Filho HFL Ribeiro GMF de Camargo DF Cardoso RR Aspilcueta‐Borquis H Tonhati KB Nunes WG Vale EM Barbosa KC de Sousa 《Reproduction in domestic animals》2013,48(5):705-709
The osteopontin gene may influence the fertility of water buffaloes because it is a protein present in sperm. The aim of this work was to identify polymorphisms in this gene and associate them with fertility parameters of animals kept under extensive grazing. A total of 306 male buffaloes older than 18 months, from two farms, one in the state of Amapá and the other in the state of Pará, Brazil were used in the study. Seven SNPs were identified in the regions studied. The polymorphisms were in gene positions 1478, 1513 and 1611 in the region 5′upstrem and positions 6690, 6737, 6925 and 6952 in the region amplified in intron 5. The SNPs were associated with the traits, namely scrotal circumference, scrotal volume, sperm motility, sperm concentration and sperm pathology. There were significant SNPs (p < 0.05) for all the traits. The SNP 6690 was significant for scrotal circumference, sperm concentration, sperm motility and sperm pathology and the SNP 6737 for scrotal volume. The genotype AA of SNP 6690 presented the highest averages for scrotal circumference, sperm concentration and motility and the lowest total number of sperm pathologies. For the scrotal volume trait, the animals with the largest volume were correlated with the presence of the genotype GG of SNP 6737. These results indicate a significance of the osteopontin gene as it seems to exert a substantial influence on the semen production traits of male buffaloes. 相似文献
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Basmah M. Alharbi Abeer A. Mahmoud Hussein A. H. Said-Al Ahl 《Journal of plant nutrition》2013,36(18):2395-2405
AbstractA field experiment was conducted on three cultivars (Italian Giant, Italian Plain, and Local) of parsley to compare plant growth, herb fresh weight, and essential oil (EO) content, yield and composition at three harvest times (Day 1, 47, and 91) in response to phosphorus (P) application rates (0, 12, 24, and 36?kg ha?1). Repeated measures analysis revealed that the ideal P rate for the growth of parsley and its EO yield is 24?kg ha?1; whereas the second harvest gives the highest height and weight; and the third harvest gives the highest EO content and yield. At all harvests, the highest EO content was obtained from Italian Giant fertilized with 36?kg ha?1 P. β-myrcene, 1,3,8-p-menthatriene, β-phellandrene, and myristcin were the major compounds in all three cultivars, but their ideal P fertilization and harvest time varied with cultivar. This study showed biomass, EO content and yield, and the accumulation of EO constituents of parsley cultivars are influenced by P application and harvest date in different ways. 相似文献
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Saif Alharbi William R. Raun Daryl B. Arnall Hailin Zhang 《Journal of plant nutrition》2019,42(6):673-679
The process of improving crop management inputs by use of remote sensing devices is a new technology. This study presents the use of the normalized-difference vegetative index (NDVI) combined with the coefficient of variation (CV) to predict plant populations in corn (Zea mays L.) over different growth stages and different locations. About 76 plots were selected from the two sites to conduct this research. The results showed that we were not able to reliably predict plant population at lately growth stages because the canopy covered the soil with overlapping leaves. However, at early growth stages, the results suggest that as the corn plant emerged from the soil, the biomass per unit area was small and the sensor technology application for prediction of plant population was possible. Further studies may be designed based on this observation to investigate the possibility at early growth stages such as V2 and V3. 相似文献
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Identification of lactoferrin and glutamate receptor‐interacting protein 1 in bovine cervical mucus: A putative marker for oestrous detection 
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下载免费PDF全文 WY Lee MH Park KW Kim H Song KB Kim CS Lee NK Kim JK Park BC Yang KB Oh GS Im HJ Chung 《Reproduction in domestic animals》2017,52(1):16-23
Accurate detection of oestrus is important for artificial insemination. The aim of this study was to identify oestrous‐specific bovine cervical mucus proteins that could be used to determine the optimal time for artificial insemination. Non‐oestrous and controlled internal drug release (CIDR)‐induced oestrous‐stage mucus proteins were purified and subjected to surface‐enhanced laser desorption/ionization time‐of‐flight mass spectrometry, sodium dodecyl sulphate polyacrylamide gel electrophoresis and MALDI‐TOF/TOF. Among differentially expressed proteins, lactoferrin (LF) and glutamate receptor‐interacting protein 1 (GRIP1) showed a twofold increase during the CIDR‐induced oestrous stage compared to the levels in non‐oestrous stage in bovine cervical mucus. The RT‐PCR, Western blotting and immunohistochemistry results showed that LF and GRIP1 expression was significantly increased during the oestrous stage in the uterus. This study demonstrated that bovine LF and GRIP1 exist during the oestrous stage, but not during the non‐oestrous stage, suggesting that cervical mucus LF and GRIP1 are useful oestrous detection markers in cattle. 相似文献
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