<Emphasis Type="Italic">Lupinus albus</Emphasis> Conglutin Gamma Modifies the Gene Expressions of Enzymes Involved in Glucose Hepatic Production <Emphasis Type="Italic">In Vivo</Emphasis>     

Ana E. González-Santiago  Belinda Vargas-Guerrero  Pedro M. García-López  Alma L. Martínez-Ayala  José A. Domínguez-Rosales  Carmen M. Gurrola-Díaz 《Plant foods for human nutrition (Dordrecht, Netherlands)》2017,72(2):134-140

Lupinus albus seeds contain conglutin gamma (Cγ) protein, which exerts a hypoglycemic effect and positively modifies proteins involved in glucose homeostasis. Cγ could potentially be used to manage patients with impaired glucose metabolism, but there remains a need to evaluate its effects on hepatic glucose production. The present study aimed to analyze G6pc, Fbp1, and Pck1 gene expressions in two experimental animal models of impaired glucose metabolism. We also evaluated hepatic and renal tissue integrity following Cγ treatment. To generate an insulin resistance model, male Wistar rats were provided 30% sucrose solution ad libitum for 20 weeks. To generate a type 2 diabetes model (STZ), five-day-old rats were intraperitoneally injected with streptozotocin (150 mg/kg). Each animal model was randomized into three subgroups that received the following oral treatments daily for one week: 0.9% w/v NaCl (vehicle; IR-Ctrl and STZ-Ctrl); metformin 300 mg/kg (IR-Met and STZ-Met); and Cγ 150 mg/kg (IR-Cγ and STZ-Cγ). Biochemical parameters were assessed pre- and post-treatment using colorimetric or enzymatic methods. We also performed histological analysis of hepatic and renal tissue. G6pc, Fbp1, and Pck1 gene expressions were quantified using real-time PCR. No histological changes were observed in any group. Post-treatment G6pc gene expression was decreased in the IR-Cγ and STZ-Cγ groups. Post-treatment Fbp1 and Pck1 gene expressions were reduced in the IR-Cγ group but increased in STZ-Cγ animals. Overall, these findings suggest that Cγ is involved in reducing hepatic glucose production, mainly through G6pc inhibition in impaired glucose metabolism disorders.  相似文献   

Phenological and physiological evaluation of first and second cropping periods of sorghum and maize crops     

Maria Antonia Machado Barbosa  Kacilda Naomi Kuki  Pedro Santos Peno Bengala  Emilly dos Santos Pereira  Angélica Fátima de Barros  Sebastián Giraldo Montoya  Leonardo Duarte Pimentel 《Journal of Agronomy and Crop Science》2020,206(2):263-276

Environmental conditions influence phenology and physiological processes of plants. It is common for maize and sorghum to be sown at two different periods: the first cropping (spring/summer) and the second cropping (autumn/winter). The phenological cycle of these crops varies greatly according to the planting season, and it is necessary to characterize the growth and development to facilitate the selection of the species best adapted to the environment. The aim of this study was to characterize phenological phases and physiological parameters in sorghum and maize plants as a function of environmental conditions from the first cropping and second cropping periods. Two parallel experiments were conducted with both crops. The phenological characterization was based on growth analyses (plant height, leaf area and photoassimilate partitioning) and gas exchange evaluations (net assimilation rate, stomatal conductance, transpiration and water-use efficiency). It was found that the vegetative stage (VS) for sorghum and maize plants was 7 and 21 days, respectively, longer when cultivated during the second cropping. In the first cropping, the plants were taller than in the second cropping, regardless of the crop. The stomatal conductance of sorghum plants fluctuated in the second cropping during the development period, while maize plants showed decreasing linear behaviour. Water-use efficiency in sorghum plants was higher during the second cropping compared with the first cropping. In maize plants, in the second cropping, the water-use efficiency showed a slight variation in relation to the first cropping. It was concluded that the environmental conditions as degree-days, temperature, photoperiod and pluvial precipitation influence the phenology and physiology of both crops during the first and the second cropping periods, specifically cycle duration, plant height, leaf area, net assimilation rate, stomatal conductance and water-use efficiency, indicating that both crops respond differentially to environmental changes during the growing season.  相似文献   

The effect of feeding a novel multistrain yeast fraction on European seabass (Dicentrachus labrax) intestinal health and growth performance     

Mark D. Rawling  Nicola Pontefract  Ana Rodiles  Ilektra Anagnostara  Eric Leclercq  Marion Schiavone  Mathieu Castex  Daniel L. Merrifield 《Journal of the World Aquaculture Society》2019,50(6):1108-1122

Fish were fed a single‐strain yeast fraction (SsYF; 2 g/kg) or a multistrain yeast fraction (MsYF; 0.8 g/kg) for 10 weeks. The results demonstrated significant (p ≤ 0.03) elevations in weight gain, specific growth rate, protein efficiency ratio, and feed conversion ratio in fish fed the yeast fraction‐supplemented diets. In the distal intestine, a significant elevation in microvilli density was observed after 5 and 10 weeks of dietary supplementation with MsYF and SsYF, respectively, compared to control fed fish (p < 0.001). A significant elevation (p = 0.02) in the perimeter ratio was observed in fish fed diets supplemented with the yeast fractions. After 10 weeks of feeding on the experimental diets, Rt‐qPCR demonstrated a significant downregulation (p < 0.05) in the stress response genes, heat‐shock protein 70 (hsp70) and proliferating cell nuclear antigen (pcna), in fish fed diets supplemented with the yeast fractions. Significant (p < 0.05) elevations in interleukin 1‐beta (il1β) and interleukin‐10 (il10) gene expression were observed in fish fed diets supplemented with the MsYF compared to the other dietary groups. These findings suggest that feeding an MsYF specifically at a lower incorporation rate < 1 g/kg, compared to a commercial SsYF at 2 g/kg, is effective in improving the intestinal health status and growth performance of European seabass.  相似文献   

Innovation strategies for smart specialisation (RIS3): Past,present and future research     

Joo Lopes  Joo J. Ferreira  Luís Farinha 《Growth and change》2019,50(1):38-68

The recent change in the regional development strategy of the European Union (EU) results in an important need to study the terms of change and their respective implications. This study seeks, through a bibliometric analysis, to ascertain the developments taking place in studies on research and innovation strategies for smart specialisation (RIS3) to identify shortcomings and opportunities for future research. This bibliometric review drew upon the Scopus database with the sample selected containing all the articles containing the keywords “Research and Innovation Strategies for Smart Specialisation” or “RIS3.” This correspondingly reports how some authors maintain that the path the EU should take involves imitative innovation. Meanwhile, others propose that each region should specialise in those industries that are already established there and thus avoid targeting any areas they do not already know/specialise in. Our findings detail six clusters in RIS3 research, which help in contextualising the literature review: (a) business discovery; (b) smart specialisation; (c) innovation; (d) specialisation; (e) regional policies; and (f) regional development. This study furthermore sets out perspectives for future lines of research and correspondingly seeks to convey a vast theoretical basis that may serve as the point of departure for future studies.  相似文献   

Backshoring and nearshoring: An overview     

Benedetta Piatanesi  Josep‐Maria Arauzo‐Carod 《Growth and change》2019,50(3):806-823

Increasingly, economies that have traditionally benefited from offshoring are losing some of their strategical advantages, with a consequent increase in backshoring by developed economies. This paper describes the phenomenon and tries to shed light on the current challenges, trends, and debates in the area, and on the main determinants of backshoring. A new phenomenon known as nearshoring is also analysed—this consists of relocating some previously offshored manufacturing activities so that they are now close to previous core locations, but not so close as to suffer from disagglomeration effects. This combines the advantages of offshoring and backshoring.  相似文献   

Development of colchicine‐induced tetraploid St. Augustinegrass (Stenotaphrum secundatum) lines     

Esdras M. Carbajal  M. Carolina Zuleta  Luellen Swayzer  Brian M. Schwartz  Maria Carolina Chavarro  A. Carolina Ballen‐Taborda  Susana R. Milla‐Lewis 《Plant Breeding》2019,138(6):958-966

St. Augustinegrass is well suited for lawns and commercial landscapes. While many genotypes are cross‐fertile, all cultivars are propagated vegetatively in sod production. To ensure varietal purity, development of sterile triploid hybrids by crossing tetraploid and diploid genotypes has been successfully used in other warm‐season turfgrasses. Applying this model in St. Augustinegrass would be beneficial to sod producers and turf managers who require purity for certification and uniformity for performance, respectively. This study was conducted to develop colchicine‐induced tetraploid lines of St. Augustinegrass. Seeds of cultivar ‘Raleigh’ were treated with four colchicine concentrations at four exposure times. A non‐treated control was included among the treatments. Seedlings that germinated were screened for genome size changes using flow cytometry. Line DSA 13005 and two progeny lines derived through selfing, DSA 16001 and DSA 16016, were corroborated as tetraploids (2n = 4x = 36) through chromosome counts. These lines will be used in future breeding efforts to attempt development of sterile triploid cultivars of St. Augustinegrass.  相似文献   

Paternal genomotype affects female growth in a hybrid fish complex          下载免费PDF全文

Sara Carona  Maria J. Collares‐Pereira  M. Filomena Magalhães 《Ecology of Freshwater Fish》2017,26(4):719-721

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Food Value of Four Microalgae for Juveniles of the Lion's Paw Scallop Lyropecten subnodosus (Sowerby, 1833)     

Maria Concepción  Lora-Vilchis Miguel  Robles-Mungaray Natalia  Doktor Domenico  Voltolina 《Journal of the World Aquaculture Society》2004,35(2):297-304

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Leishmaniasis phytotherapy. Nature's leadership against an ancient disease.     

P B de Carvalho  E I Ferreira 《Fitoterapia》2001,72(6):599-618

The use of phytotherapy to treat human diseases has its roots in pre-historical times. Despite the modern advances achieved in the field of synthetic chemistry, the most efficient drugs available have their genesis directly or indirectly related with the vegetal kingdom. Indigenous communities have long used plant extracts to treat illnesses. Many of these extracts have shown effective action, with new bioactive compounds being extracted and screened every year. These extracts have also proven to be good sources of therapeutic agents to the treatment of Leishmaniasis. This work highlights some of these agents, while trying to emphasize the importance of plants as a source of new and powerful drugs against this widespread disease.  相似文献   

Modeling multi-scale spatial ecological processes under the discrete event systems paradigm     

Maria J. Perestrello de Vasconcelos  Bernard P. Zeigler  Lee A. Graham 《Landscape Ecology》1993,8(4):273-286

A multi-scale spatial ecological model of a wet sclerophyllous forest subject to recurrent fires is presented. The model is specified in a Discrete Event Systems framework (DEVS) (Zeigler, 1990) interfaced with a Geographic Information System (GIS), and includes the ability to simulate landscape dynamics at several levels of resolution simultaneously. This is achieved by encoding a modular hierarchical representation of the forest landscape components into a set of nested, interconnected, and spatially referenced dynamic models. The results of the landscape dynamics simulations are displayed as sequences of maps through time, illustrating the potential of this modeling methodology for dealing with complex hierarchical structures that operate at several spatial and temporal resolutions.Research work supported by Junta Nacional de Investigaçäo Científica e Tecnológica, Portugal, and the Advanced Resource Technology Program, University of Arizona, USA.  相似文献