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1.
Metlholo Andries Phukuntsi Helene Brettschneider Desiré L Dalton Teresa Kearney Jacqueline Badenhorst Antoinette Kotze 《African Zoology》2016,51(1):69-76
DNA barcoding has been proposed as a method for species identification. However, this method has been criticised for its over-reliance on a single mitochondrial gene. In this study, four mitochondrial gene regions and one nuclear gene region were used to investigate their different abilities to identify tissue associated with museum specimens of Aethomys chrysophilus, Aethomys ineptus and Micaelamys namaquensis. Aethomys chrysophilus and the more recently elevated A. ineptus are indistinguishable on morphological grounds; however, their ranges are largely parapatric with only one syntopic locality currently known. All of the mitochondrial gene regions were able to separate M. namaquensis from A. chrysophilus and A. ineptus, but they varied in their abilities to resolve differences between A. chrysophilus and A. ineptus. The sequence results identified a specimen from KwaZulu-Natal that was misclassified and should have been identified as A. ineptus. Seven specimens that had not been reclassified following the elevation of A. ineptus to species level were identified as A. ineptus. Individuals of A. chrysophilus from Malawi could not be classified as either A. chrysophilus or A. ineptus, and may be a hybrid or a new, distinct species. This study indicates that DNA barcoding may be used to separate M. namaquensis from A. chrysophilus and A. ineptus, and although it was not able to separate A. chrysophilus and A. ineptus, it did indicate specimens from Malawi may be a new cryptic species. 相似文献
2.
The African Penguin (Spheniscus demersus) has suffered population declines and is listed in the IUCN Red List as Endangered. The species is endemic to the coast of southern Africa, and breeding colonies are distributed on the south-western coast of Africa. Currently, African Penguins are being kept in zoo and aquarium facilities throughout South Africa. In this study, molecular genetic data based on 12 microsatellite markers from 1 119 African Penguin samples from four facilities were generated in order to determine the level of genetic variation, population structure and differentiation, and effective population size to assist in the development of an effective captive management plan. Expected heterozygosity ranged from 0.57 to 0.62, and allelic richness from 4.2 to 5.1. However, based on differences between first- and second-generation captive birds, we conclude that the ex situ population is at risk of losing genetic variability in the future and management programmes should include exchange of birds between captive facilities in order to induce gene flow and increase effective population size. Adding individuals from in situ populations should also be considered in the future in cases where these birds cannot be rehabilitated. Molecular genetic analyses of wild penguin populations should be carried out for comparison, and to ascertain to what degree ‘in situ genetic diversity’ is represented among ex situ populations. With regular resampling and analyses, the extent of the effect of processes such as genetic drift on diversity in the ex situ penguin populations will become evident. 相似文献
3.
An immunohistochemical study of various peptide-containing endocrine cells and neurones at the equine ileocaecal junction 总被引：1，自引：0，他引：1
The ileocaecal junctions of 5 horses and 2 donkeys were examined by using antisera to the following peptides: somatostatin, glucagon, gastrin, neurotensin, vasoactive intestinal peptide (VIP), peptide histidine isoleucine (PHI), calcitonin gene-related peptide (CGRP), substance P (SP) and neuropeptide Y (NPY). Antisera to somatostatin, neurotensin and NPY demonstrated endocrine cells in the ileal- and caecal parts of the ileocaecal junction, while immunoreactivity for glucagon was demonstrated in endocrine cells of the ileal part only. Nerve cell bodies showing immunoreactivity to SP, VIP, CGRP and PHI were demonstrated in the myenteric and submucosal plexuses and were associated with small blood vessels in the submucosa of all the regions tested. Ramified nerve fibres in the submucosa immunoreactive to SP, VIP, CGRP and PHI extended to the mucosa and to small blood vessels in the submucosa. Nerve fibres showing immunoreactivity to SP, VIP and PHI extended to the circular smooth muscle layer of the ileocaecal junction. 相似文献
4.
Antoinette M. G. A. WinklerPrins Narciso Barrera-Bassols 《Agriculture and Human Values》2004,21(2-3):139-156
Ethnopedology is the study of local knowledge of soil and land management in an ecological perspective. It is an emerging hybrid discipline that is a component of ethnoecology and stands to offer much for land-based studies. This paper reviews the field of ethnopedology in Latin America and compares some of the many case studies from that region. Various literature sources are considered, including the ethnographical, ethnohistorical, archaeological, geographical, agronomic, ethnoecological, and development studies. Our review invokes the theory of ethnoecology that focuses on the linkages between kosmos (beliefs and symbolic representations), corpus (environmental knowledge), and praxis (the set of practical operations through which the material appropriation of nature takes place) of local land-users. The main topics covered are the ethnohistorical and archaeological evidence of ethnopedology, local soil and land classification, local land management systems, local perceptions and beliefs of soil and land resources, and local soil fertility management. After analysing past and present research trends, recommendations are given on how ethnopedological studies can contribute to enhance sustainable land use and management in Latin America. 相似文献
5.
Fortunel NO Otu HH Ng HH Chen J Mu X Chevassut T Li X Joseph M Bailey C Hatzfeld JA Hatzfeld A Usta F Vega VB Long PM Libermann TA Lim B 《Science (New York, N.Y.)》2003,302(5644):393; author reply 393
6.
Dawes ME Lakritz J Tyler JW Cockrell M Marsh AE Estes DM Larson RL Steevens B 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2004,18(1):104-108
Lactoferrin (LF) is an iron-binding protein present in both colostrum and secondary granules of polymorphonuclear neutrophils (PMNs). We hypothesized that supplemental LF enhances neutrophil function in neonatal calves. Newborn calves were assigned to receive colostrum (C), colostrum + LF (CLF, 1 g/kg), or milk replacer + LF (MRLF, 1 g/kg). Serum (LF and IgG) and whole blood (neutrophil isolation) samples were obtained prior to treatment (day 0) and at 24 hours and 9 days of age. Serum IgG concentrations (mean +/- SD) in C, CLF, and MRLF calves at 24 hours were 1,911 +/- 994 mg/dL, 2,181 +/- 625 mg/dL, and 0 mg/ dL, respectively. Serum LF concentrations in C, CLF, and MRLF calves on day 0 were 324 +/- 334 ng/mL (range 0-863 ng/mL), 135 +/- 158 ng/mL (range 0-429 ng/mL), and 318 +/- 337 ng/mL (range 0-964 ng/mL), respectively. LF concentrations in C, CLF, and MRLF calves at 24 hours were significantly higher (P < .05), at 1,564 +/- 1,114 ng/mL (range 335-3,628 ng/mL, 2,237 +/- 936 ng/mL (range 31-3,287 ng/mL), and 3,189 +/- 926 ng/mL (range 1,736-4,120 ng/mL), respectively. Cytochrome c reduction in opsonized zymosan-treated or phorbol ester-treated cells was not significantly affected by supplemental LF provided at birth. Oral LF is absorbed in calves but does not alter PMN superoxide production and does not alter IgG absorption. 相似文献
7.
D L Barnes N F G Rethman C C De Witt G D Kotze 《African Journal of Range and Forage Science》2013,30(3):90-95
Abstract The feasibility and potential of reinforcing veld with legumes in the south‐eastern Transvaal highveld was assessed in a number of trials during the period 1974–84. Experience was gained with regard to methods of establishment, adaptation of legumes to the local environment, responses to fertilization, especially with lime and superphosphate, and the reaction of legumes to fire and grazing. In all, 17 legumes were involved, including the genera Coronilla, Desmodium, Glycine, Lespedeza, Lotus, Macroptilium, Medicago, Neoto‐nonia, Trifolium and Vigna. Only Coronilla varia (crownvetch) showed a satisfactory degree of persistence. However, crownvetch is slow to establish, is intolerant of waterlogging and has a relatively high requirement for lime and phosphate. Considering these features, and the probability of undesirable changes in grass species composition in reinforced veld, it is recommended that intensification be achieved through development of improved pastures for use in conjunction with veld, rather than by means of veld reinforcement. 相似文献
8.
Adama Sanou Amadou Dicko Kadiatou R. Sow Arthur Djibougou Antoinette Kabore Bassirou Diarra Arsne K. Ouedraogo Dezemon Zingue Moumini Nouctara Zekiba Tarnagda 《The Onderstepoort journal of veterinary research》2021,88(1)
Bovine tuberculosis (bTB) is a zoonotic, infectious, chronic and contagious disease, caused by Mycobacterium bovis that mainly affects cattle. This pathology has a negative impact on animals and animal products trade. Unfortunately, in Burkina Faso where agriculture and livestock sectors represent around 80% of the socio-economic activities, the real situation of the disease is not well known especially in small ruminants and swine. Thus, our study focused on both the epidemiology and the microbiological diagnosis of tuberculosis (TB) in small ruminants and pigs slaughtered at Bobo-Dioulasso abattoir. A prospective study was conducted between August 2017 and December 2017. Epidemiological data collection was performed during routine meat inspection; moreover, samples were taken and transported to the Bacteriology laboratory of Centre Muraz for microbiological analyses. This diagnosis consisted in search of Acid Fast Bacilli (AFB) using the hot Ziehl–Neelsen staining. Out of a total of 14 648 small ruminants and 2430 pigs slaughtered during the study period, 156 and 17 had lesions suggestive of bTB with prevalence of 1.07% and 0.7%, respectively. Females and those between 2 and 4 years old were mainly infected. The most affected organs were: lungs, liver, spleen and lymph nodes. Finally, microscopy revealed 43.35% (75/173) of positive cases for AFB. These results confirm the presence of bTB in small ruminants and pigs in Burkina Faso. Efforts must still be made in the fight against this zoonosis in order to limit its economic and public health impacts. 相似文献
9.
Early growth of two grades ofbare-root Pinus radiata D.Don seedlingswere studied in response to four soilcultivation treatments and two weed controltreatments.Soil cultivation treatments included (i) pitsmade by hand, (ii) pits made with an auger,(iii) ripping alone, and (iv) ripping plusdisking. Weed treatments included (i) manualrelease 1 year after planting or (ii) totalweed control for 1 year involving the use ofherbicides and additional hoeing. Bare-rootseedlings were separated into either medium(4.1-mm average root-collar diameter; height25–31 cm) or small (2.8-mm average root-collardiameter; height 14–20 cm) size classes.Growth on this site was excellent and 7 yearsafter treatment, trees in the least expensivetreatment averaged 12.8 m in height. Use ofmedium seedlings and extra weed controlincreased merchantable volume by20 m3 haminus 1 and 19 m3 haminus 1,respectively. When combined, the increase was39 m3 haminus 1. However, none of the soilcultivation treatments caused a significantincrease in merchantable volume. Disking onthis site proved to be of no benefit. Aboundary-line analysis was used to examine themarginal returns from investing in intensivesilviculture. 相似文献
10.
The Cape dune mole-rat, Bathyergus suillus, is the largest truly subterranean rodent in the world and is restricted to sandy soil areas of the southern and South-western Cape Province. These animals are herbivorous, feeding mostly on the below ground portions of grass and geophytes. The present study was undertaken to explore whether there are any gross anatomical gastrointestinal adaptations associated with the herbivorous diet of the Cape dune mole-rat and to provide a basis for further investigations into the nutrition and ecology of this animal. The stomach was unilocular (simple) and the entire internal aspect displayed macroscopically visible glandular epithelium. The caecum was voluminous and arranged into a spiral of approximately one and a half turns. The tip of the caecum resembled a vermiform appendix and lay against the left lateral abdominal wall. The ascending colon consisted of proximal and distal portions, arranged in a large double loop and attached to each other by a fat-filled mesenteric fold. The looped, folded ascending colon filled the right side of the abdominal cavity, lying against the right dorsal, lateral and ventral abdominal wall. The internal structure of the ascending colon revealed a shallow groove, lined on either side by a row of papillae. The simple, glandular stomach in conjunction with a prominent caecum and a greatly enlarged ascending colon suggests that this animal is a hindgut fermenting herbivore, rather than a caecal fermenter, as is the case in most rodent species. 相似文献