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Worapa Seehalak Norihiko Tomooka Aree Waranyuwat Piyada Thipyapong Paisan Laosuwan Akito Kaga Duncan A. Vaughan 《Genetic Resources and Crop Evolution》2006,53(5):1043-1059
Thailand is a center of diversity for section Angulares of the Asian Vigna (genus Vigna subgenus Ceratotropis) and 4 Vigna species are cultivated in Thailand. Using newly collected wild and cultivated germplasm of Vigna from Thailand and outgroup accessions AFLP analysis was conducted to clarify genetic diversity and relationships. The results
suggest that cultivated V. umbellata and V. mungo evolved from wild relatives in a single domestication event. Vigna umbellata is poorly differentiated from its wild and weedy relatives compared to V. mungo. Results suggest northern Thailand and the neighboring Shan state, Myanmar, is the probable center of domestication for V. umbellata as wild accessions from this area and cultivated rice bean from a wide area in Asia are not greatly diverged. Vigna minima, V. tenuicaulis and V. exilis accessions in Thailand are well differentiated with considerable intra-specific variation. Vigna hirtella consists of two well differentiated subgroups, suggesting taxonomic revision may be necessary. Close genetic relationships
between V. radiata and V. grandiflora, and between V. mungo and V. trinervia are confirmed. Naturally growing V. mungo populations in northern Thailand appear to be true wild species as they are well differentiated from Indian wild and Thai
cultivated populations. The origin of naturally growing cowpea in Thailand needs to be further studied using a more comprehensive
set of materials. This study clarifies inter and intra-specific genetic diversity and inter species relationships of Thai
Vigna species. 相似文献
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Kanittha Phetudomsinsuk Kaitkanoke Sirinarumitr Aree Laikul Anuchai Pinyopummin 《Acta veterinaria Scandinavica》2008,50(1):41
Background
One of the semen quality parameters use to determine fertility is the percentage of sperm that express normal morphology. Sperm head morphometry is also correlated with fertility. The objectives of this study were 1) to investigate the sperm morphology and normal sperm head morphometry of Thai native crossbred stallions, and 2) to compare our results with the characteristics of proven fertile sperm from purebred stallions.Methods
Semen samples were collected monthly from nine stallions, of which five were Thai native crossbred (T) and four were purebred of proven fertility (F: F1 was a Standard-bred; F2 was a Warm-blood; F3 and F4 were Thoroughbreds). All the animals were aged between 5 and 12 years. Sperm morphological examination was performed using formaldehyde-fixed samples under phase-contrast microscopy (1000×). Normal sperm head morphometry characteristics were measured by Computer-Assisted Semen Analysis (Hamilton Thorne, USA.) after applying the Harris'' haematoxylin staining technique.Results
The percentages of morphologically normal and abnormal sperm varied among individual stallions in both the T and F groups. The mean percentage of morphologically normal sperm was not significantly different (P > 0.05) between T and F stallions (mean ± SE, 49.7 ± 1.3 and 48.1 ± 2.8, respectively). A comparison between the T and F sperm heads revealed that all the dimensional parameters were significantly different (P < 0.05). The coefficients of within-animal variation (CVs) ranged from 2.6 (shape factor 1) to 7.5 (elongation) and 2.9 (shape factor 1) to 8.1 (elongation) in T and F, respectively. In the case of the T group, those sperm head parameters that featured a low within-animal CV and a high between-animal CV were perimeter (2.9, 19.1), shape factor 1 (2.6, 25.8) and shape factor 3 (3.8, 32.0). In the case of the F group, only shape factor 1 (2.9, 26.1) featured such characteristics.Conclusion
We found variability in the percentage of morphologically normal and abnormal sperm, as well as in sperm head dimensions among Thai native crossbred stallions, and these results were similar to those of purebred stallions. Our findings demonstrate that the heads of the T sperm specimens were larger and rounder than that of the F sperm. Perimeter, shape factor 1 and shape factor 3 could be used as parameters for the identification of individual T stallions based on a sperm sample. 相似文献5.
Metita Sussadee Janjira Phavaphutanon Kornchai Kornkaewrat Aree Thayananuphat 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(1):67-74
The purpose of the present study was to establish normal electroretinogram (ERG) parameters using 56 normal eyes of four dog breeds common in Thailand: poodle, Labrador retriever, Thai ridgeback, and Thai Bangkaew. Standard ERG findings were bilaterally recorded using a handheld multi-species ERG unit with an ERG-jet lens electrode for 28 dogs under preanesthesia with diazepam, anesthesia with propofol, and anesthesia maintenance with isoflurane. There were significant differences in the mean values of ERG amplitudes and implicit times among the four dog breeds (p < 0.05) except for the b-wave implicit time of the photopic 30 Hz flicker response with 3 cd.s/m2 (p = 0.610). Out of the four breeds, Thai Bangkaew had the longest implicit time (p < 0.001) of scotopic low intensity responses, b-wave of scotopic standard intensity responses (3 cd.s/m2), a-wave of the higher intensity response (10 cd.s/m2), and a-wave of the photopic single flash response (3 cd.s/m2). For the b/a ratio, only the ratio of the Cone response was significantly different among the different breeds. In this summary, normal ERG parameters for four dog breeds were reported. Data from the investigation supported the hypothesis that determination of breed-specific limits of normality for ERG responses is necessary for individual clinics and laboratories. 相似文献
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Kim IY Shin JH Kim HS Lee SJ Kang IH Kim TS Moon HJ Choi KS Moon A Han SY 《The Journal of reproduction and development》2004,50(2):245-255
Pyrethroid insecticides are among the most commonly used classes of insecticides worldwide, but their endocrine disrupting activities remain unclear. Therefore, in the present study, we examined the estrogenic activities of pyrethroid insecticides in E-screen and competition binding assays. In addition, we measured estrogen receptor (ER) protein and pS2 mRNA levels in human breast cancer cells (MCF-7 BUS) to clarify the mechanism of their estrogenicity. Seven pyrethroid insecticides (bioallethrine, cypermethrin, deltamethrin, fenvalerate, permethrin, sumithrin, and tetramethrin) were tested because of their worldwide usage. In addition, 17beta-estradiol was tested as a positive control. As expected, 17beta-estradiol significantly increased MCF-7 BUS cell proliferation at concentrations of 10(-11) M and above. Of the pyrethroid insecticides tested, only sumithrin increased MCF-7 BUS cell proliferation in a dose-dependent manner; the maximum induction of cell proliferation was observed at a dose of 10(-5) M. In the anti-estrogenic activity test, bioallethrin, fenvalerate, and permethrin significantly inhibited 17beta-estradiol-induced MCF-7 BUS cell proliferation at 10(-6) M, a concentration comparable to the effective dose (10(-9) M) of ICI 182,780, a pure ER antagonist. However, none of the pyrethroid insecticides competitively inhibited the binding of [(3)H]estradiol to rat uterus ERs in competition binding assays. Both 17beta-estradiol (10(-10) M) and sumithrin (10(-5) M) decreased the levels of cytosolic ERalpha and ERbeta protein expression significantly as compared with the vehicle control. In addition, 17beta-estradiol (10(-10) M) increased pS2 mRNA expression markedly, and sumithrin significantly increased pS2 mRNA levels in a dose-dependent manner. The other six compounds tested in the present study did not affect ER protein levels or pS2 mRNA levels. These results suggest that certain pyrethroid insecticides may be considered to be estrogen-like chemicals that act through pathways other than direct ER binding, and may function as endocrine modulators in both wildlife and humans. 相似文献
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T. Moonmanee C. Navanukraw S. Uriyapongson A. Kraisoon S. Aiumlamai S. Guntaprom T. Rittirod P.P. Borowicz D.A. Redmer 《Domestic animal endocrinology》2013
To determine the relationships among vasculature, mitotic activity, and expression of endothelial nitric oxide synthase (eNOS) of antral follicles in Bos indicus, bovine ovaries were obtained on day 6 of the estrous cycle from 10 crossbred (Brahman to Thai native cows) after a synchronized estrus with prostaglandin F2α analogue. Ovaries were fixed, paraffin-embedded, and used for immunofluorescence detection of factor VIII (a marker of endothelial cells). Immunostaining of eNOS and proliferating cell nuclear antigen (PCNA) were performed with specific monoclonal antibodies. Vasculature and positive staining of eNOS and PCNA were quantitatively evaluated with the image analysis. Follicles were classified by size (small, medium, and large) and by structure as healthy and atretic follicles (n = 82). The expression of factor VIII and eNOS were detected greater in the blood vessels of the theca layers of the healthy follicles than those in atretic follicles. The labeling indices (LIs) in granulosa and theca cells were greater (P < 0.05) in the healthy small and medium follicles than in the healthy large follicles. Vasculature, capillary area density, and capillary number density were positively correlated with eNOS expression and the LIs of granulosa and theca cells but were negatively correlated with the healthy follicle size. During the growing phase of antral follicle in Bos indicus, relationships among vasculature, mitotic activity, and eNOS were observed predominantly in healthy antral follicles. Thus, these data highlight the importance of vasculature, cell proliferation, and eNOS expression of growing and atretic follicles in the first follicular wave. 相似文献
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Suthatip Ngokpol Wittaya Suwakulsiri Sanya Sureram Kriengsak Lirdprapamongkol Thammarat Aree Suthep Wiyakrutta Chulabhorn Mahidol Somsak Ruchirawat Prasat Kittakoop 《Marine drugs》2015,13(6):3567-3580
Four new sesquiterpene lactones (3, 4, 6 and 7) and three known compounds, purpuride (1), berkedrimane B (2) and purpuride B (5), were isolated from the marine fungus, Talaromyces minioluteus (Penicillium minioluteum). New compounds were drimane sesquiterpenes conjugated with N-acetyl-l-valine, and their structures were elucidated by analysis of spectroscopic data, as well as by single crystal X-ray analysis. The isolated compounds could not inhibit the apoptosis-regulating enzyme, caspase-3, while three of the compounds (2, 3 and 7) exhibited weak cytotoxic activity. 相似文献
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Chainarong Navanukraw Vilaivan Khanthusaeng Aree Kraisoon Suthipong Uriyapongson 《Tropical animal health and production》2014,46(8):1441-1446
The study was conducted to compare estrous rate, ovulatory response, plasma progesterone concentrations, and conception rate following cervical artificial insemination in goats given a new or once-used controlled internal drug release (CIDR) device with human chorionic gonadotropin (hCG). Fifty-six Thai-native goats with the average age and body weight of 11 months and 17.3 kg received a 14-day treatment with a new CIDR device (Eazi-BreedTMCIDR®, Pfizer, NY, USA) or a once-used CIDR device. All goats received a 300-IU injection of hCG (Chorulon®, Intervet International B.V., New Zealand) at the day of CIDR removal to induce ovulation. All goats displaying signs of Estrous behavior were artificially inseminated at 12 h after the onset of estrus with frozen semen. No differences in percentage of estrus and ovulation rates were observed; however, goats that received once-used CIDR devices exhibited shorter (P?P?>?0.05) between treatments during CIDR device insertion and at the time of CIDR removal except on day 4. No significant differences were found in overall conception rates between the treatments. This study indicates that the once-used CIDR device with hCG could be applied to synchronize the estrus and ovulation in small-size Thai-native goats without negative effects on Estrous behavior, ovulatory response, and plasma P4 concentration. 相似文献
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Hassan Muhamed Sami Youssef Ahmed Mustafa Hishyer Suliman Aree Abdulqader Hishyer Mohammed Richard Michalet 《林业研究》2019,(5):1849-1857
Zawita natural forest has recently has been subject to mass recreational activities during spring that have denuded large areas of the forest.It was thus essential to assess regeneration before designing optimizing strategies.To this end,we studied the overstory canopy and microhabitat conditions for recruitment of Pinus brutia Ten in 10 plots(20×25 m)on the southern aspects where the Zawita natural forest is still present.In total,1540 regenerating P.brutia were recorded,854 seedlings,597 saplings,and 89 trees.Seedlings and saplings were more frequent beyond the canopy than under the canopy of the parent trees.Regeneration requirements differed between seedlings and saplings.The probability of the occurrence of seedlings was negatively correlated with increasing litter depth and increasing soil compaction.The density of saplings only showed a positive significant correlation with increasing slope.The nearest neighbor index showed a trend toward a positive spatial association between understory shrubs with their neighboring seedlings at a mean distance of 1.6 m.Overall,the study highlighted the requirements for seedling regeneration as a relatively open canopy cover,a light understory litter layer,and noncompacted soils.These results are a step towards designing effective management and restoration programs. 相似文献
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