Melatonin reduces apoptotic cells,SOD2 and HSPB1 and improves the in vitro production and quality of bovine blastocysts          下载免费PDF全文

TC Marques  EC da Silva Santos  TO Diesel  LO Leme  CF Martins  MAN Dode  BG Alves  FPH Costa  EB de Oliveira  ML Gambarini 《Reproduction in domestic animals》2018,53(1):226-236

Effects of adding different concentrations of melatonin (10^?7, 10^?9 and 10^?11 M) to maturation (Experiment 1; Control, IVM  + 10^?7, IVM  + 10^?9, IVM  + 10^?11) and culture media (Experiment 2; Control, IVC  + 10^?7, IVC  + 10^?9, IVC  + 10^?11) were evaluated on in vitro bovine embryonic development. The optimal concentration of melatonin (10^?9 M) from Experiments 1–2 was tested in both maturation and/or culture media of Experiment 3 (Control, IVM  + 10^?9, IVC  + 10^?9, IVM /IVC  + 10^?9). In Experiment 1, maturated oocytes from Control and IVM  + 10^?9 treatments showed increased glutathione content, mitochondrial membrane potential and percentage of Grade I blastocysts (40.6% and 43%, respectively). In Experiment 2, an increase in the percentage of Grade I blastocysts was detected in IVC  + 10^?7 (43.5%; 56.7%) and IVC  + 10^?9 (47.4%; 57.4%). Moreover, a lower number and percentage of apoptotic cells in blastocysts were observed in the IVC  + 10^?9 group compared to Control (3.8 ± 0.6; 3.6% versus 6.1 ± 0.6; 5.3%). In Experiment 3, the IVC  + 10^?9 treatment increased percentage of Grade I blastocysts with a lower number of apoptotic cells compared to IVM /IVC  + 10^?9 group (52.6%; 3.0 ± 0.5 versus 46.0%; 5.4 ± 1.0). The IVC  + 10^?9 treatment also had a higher mRNA expression of antioxidant gene (SOD 2) compared to the Control, as well as the heat shock protein (HSPB 1) compared to the IVM  + 10^?9. Reactive oxygen species production was greater in the IVM /IVC  + 10^?9 treatment group. In conclusion, the 10^?9 M concentration of melatonin and the in vitro production phase in which it is used directly affected embryonic development and quality.  相似文献   

Parasitological and clinical studies of experimentally cryptosporidia-infected NMRI mice     

U Eberhardt  J Boch  J Heine 《Berliner und Münchener tier?rztliche Wochenschrift》1985,98(8):266-268

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Collection of wood quality data by X-ray densitometry: a case study with three southern pines     

Thomas L. Eberhardt  Lisa J. Samuelson 《Wood Science and Technology》2015,49(4):739-753

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Restriction fragment length polymorphisms in Australian and New Zealand isolates of Leptospira interrogans serovar pomona     

BG CORNEY  J COLLEY 《Australian veterinary journal》1996,74(2):156-157

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Detection of uranium from cosmos-1402 in the stratosphere     

Leifer R  Juzdan ZR  Kelly WR  Fassett JD  Eberhardt KR 《Science (New York, N.Y.)》1987,238(4826):512-514

The nuclear reactor from the Soviet radar reconnaissance satellite, Cosmos-1402, reentered the earth's atmosphere on 7 February 1983 and disintegrated over the South Atlantic Ocean. The reactor was powered by approximately 50 kilograms of uranium-235 ((235)U). In an effort to determine the fate of the reactor core, a series of aerosol samples were collected at altitudes between 27 and 36 kilometers in the Northern Hemisphere approximately 1.1 years later by high-altitude balloons. At an altitude of 36 kilometers a 53 +/- 20 percent excess in the (235)U concentration was measured. The total excess of (235)U in the stratosphere was calculated to be 44 +/- 15 kilograms.  相似文献   

Plasma heparin values and hemostasis in equids after subcutaneous administration of low-dose calcium heparin   总被引：1，自引：0，他引：1  

H Gerhards  C Eberhardt 《American journal of veterinary research》1988,49(1):13-18

Different doses of heparin were given to equids SC to establish 0.05 to 0.20 U of heparin/ml of plasma. Plasma heparin values and antithrombin III activities were assayed, using chromogenic substrate methods. Activated partial thromboplastin and thrombin times were determined, using conventional coagulation assays. Tests were run every hour (or every 2 hours for antithrombin III) for 12 hours from 5 groups of 5 equids each after single injection of 40, 60, 80, 100, or 125 U of calcium heparin/kg of body weight and from 11 equids after injection of 150 U of calcium heparin/kg. The smaller dose (40 U/kg) did not or only insufficiently increase plasma heparin values. After 150 U of heparin/kg, plasma heparin concentrations were increased for 11 hours and were significantly (P = 0.05) increased between postadministration hours 2 and 7. The desired range beyond 0.05 U of heparin/ml was achieved between postadministration hours 2 and 6. Antithrombin III, activated partial thromboplastin time, and thrombin time were not significantly affected by any heparin dose. In cumulative studies, 2 equids were given 150 U of heparin/kg 7 times at 12-hour intervals. After the 3rd injection, a mean plasma concentration of 0.20 U of heparin/ml was achieved; after the 7th injection, the mean concentration was 0.22 U of heparin/ml. Four equids were given 150 U of heparin/kg initially, followed by 125 U of heparin/kg 6 times at 12-hour intervals. After the 3rd injection, a maximal plasma heparin concentration of 0.12 U of heparin/ml was achieved; after the 7th injection, the mean concentration was 0.18 U of heparin/ml. Repeated SC heparin injections caused moderate swelling at the injection sites.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Nitrogen rhizodeposition in agricultural crops: Methods,estimates and future prospects     

Florian Wichern  Elmar Eberhardt  Jochen Mayer  Rainer Georg Joergensen  Torsten Müller 《Soil biology & biochemistry》2008,40(1):30-48

The objective of the present review was to present the current knowledge on nitrogen (N) rhizodeposition, including techniques for ¹⁵N labelling of agricultural plants, amounts of N rhizodeposition and its fate in soil. Rhizodeposition is the process of release of organic and inorganic compounds from living plant roots. It is often quantified in terms of carbon (C) and less often as N derived from rhizodeposition (NdfR). Rhizodeposition of N can be estimated by labelling plants with ¹⁵N and following its fate in soil. Most methods used for labelling plants with ¹⁵N can only be applied after appearance of the first leaf and only allow pulse or multiple pulse labelling. Only the split-root technique and the application of gaseous ¹⁵N allow continuous labelling. All methods available at present have their flaccidities mostly due to the fact that the application of N is not following its physiological pathway of assimilation or by using artificial conditions. In the studies reviewed, amounts of N rhizodeposits ranged from 4% to 71% of total assimilated plant N. In legumes the median was 16% and in cereals it was 14%. Rhizodeposits were 15–96% of the below-ground plant biomass (BGP). In legumes the median was 73% and in cereal it was 57%. The high variability of these results shows the need for more investigations on N rhizodeposition looking especially on the factors influencing the amounts released in different plant species under field conditions.  相似文献   

Aspects on Hormonal Control of Normal and Induced Parturition in the Dog   总被引：1，自引：0，他引：1  

B Hoffmann  A Riesenbeck  D Schams  BG Steinetz 《Reproduction in domestic animals》1999,34(3-4):219-226

Average length of gestation on the dog is 63 ± 2 days but may vary between 57-71 days due to the long period of receptivity at oestrus and the extended period of sperm survival in the female genital tract. In contrast to other domestic animals progesterone- and oestrogen concentrations are almost identical in pregnant and non pregnant bitches, except for their rapid decline immediately prior to parturition. Control of luteolysis still poorly understood. Experiments with indomethacin leading to a blockade of the prepartal PgF_2α increase, which commences with the decrease of progesterone, point toward a role of PgF_2α at this stage of pregnancy, which was extended for several days. At physiological conditions first visible signs of parturition were observed at peak-PgF_2α levels, 33.6 ± 17.6 hours after onset of luteolysis, which lasted over 16.8 ± 3.4 hours. Pulse-type releases of oxytocin were only observed after this point of time. To test for the effect of progesterone-withdrawal, four 51-57 days pregnant bitches were treated with the antiprogestin RU 38486 which inhibits the activity of progesterone at the receptor level. In all dogs first visible signs of parturition were observed 33.5 ± 7.5 hours after onset of treatment. However, the process of parturition came to an end after cervical opening and totally only one puppy was born. Different to a normal parturition no increase of PgF_2α was observed. Relaxin levels were not influenced by treatment. These observations suggested that treatment with antiprogestin followed by PgF_2α might be an adequate method to induce parturition in the dog; first experiences seem to confirm this conclusion.  相似文献   

Effects of companion crops and tillage on soil phosphorus in a Brazilian oxisol: a chemical and 31P NMR spectroscopy study     

Eberhardt  Diogo  Marchão  Robélio  Quiquampoix  Hervé  Le Guernevé  Christine  Ramaroson  Volaniaina  Sauvadet  Marie  Muraoka  Takashi  Becquer  Thierry 《Journal of Soils and Sediments》2021,21(2):1024-1037

Journal of Soils and Sediments - Phosphorus (P) is often the main limiting factor for plant growth in highly weathered tropical soils. Phosphate use efficiency and crop yields could be increased in...  相似文献   

Importance of Staphylococcus hyicus ssp hyicus as a cause of arthritis in pigs up to 12 weeks of age     

BD HILL  BG CORNEY  TM WAGNER 《Australian veterinary journal》1996,73(5):179-181

Lame pigs, up to 12 weeks of age, were necropsied to establish a diagnosis. Of 175 pigs examined, 165 were confirmed to have arthritis by histopathological examination of joint tissues. Lesions were most common in the elbow and tarsal joints and least common in the joints of the feet. Typically, there was severe fibrinopurulent inflammation of synovial membranes regardless of the bacteria isolated. A bacterial aetiology was found in 114 (69%) of the 165 pigs. In arthritic pigs in which an aetiology was established the causative agents were Staphylococcus hyicus ssp hyicus (24.6%), Streptococcus equisimilis (26.3%), Actinomyces pyogenes (13.2%), Staphylococcus aureus (7.9%) and Haemophilus parasuis (7.9%). While gender did not affect the prevalence of arthritis, there was an age influence, most of the pigs culled for arthritis being under 6 weeks of age.  相似文献