Spermatic and oxidative profile of domestic cat (Felis catus) epididymal sperm subjected to different cooling times (24, 48 and 72 hours)          下载免费PDF全文

DSR Angrimani  KK Nagai  BR Rui  LC Bicudo  JDA Losano  MM Brito  MCP Francischini  M Nichi 《Reproduction in domestic animals》2018,53(1):163-170

Cooling stored epididymal samples for several days allows facilities to transport and process genetic material post‐mortem. Improvements to this practice allow the preservation of sperm from domestic cats, which are the ideal study model for wild felids. However, the modifications in spermatic features and the oxidative profile are not fully understood in cats. This information is necessary for the development of biotechniques, such as new extenders for cryopreservation. Therefore, the purpose of this study was to evaluate the spermatic and oxidative profile in samples from the epididymal cauda of domestic cats cooled at 5°C for 24, 48 and 72 hr. Spermatozoa were collected from the epididymis cauda. Evaluations consisted of computer‐assisted sperm analysis (CASA), plasma membrane integrity (eosin/nigrosin), acrosome integrity (fast green/rose bengal), sperm morphology, sperm DNA integrity (toluidine blue), mitochondrial activity (3′3 diaminobenzidine), activity of the antioxidant enzymes glutathione peroxidase (GPx) and superoxide dismutase (SOD), measurement of lipid peroxidation (TBARS) and protein oxidation. A decrease in sperm motility parameters was observed after 72 hr of cooling (i.e. total and progressive) with a higher percentage of minor (37.7 ± 6.3%) and total defects (53.4 ± 6.3%). Additionally, a decrease in high mitochondrial activity (Class I: 16.6 ± 2.2%) occurred after 72 hr. The decrease in motility rates after a long cooling time probably was caused by the increase in sperm abnormalities. A long cooling time causes cold shock and mitochondrial exhaustion, but there was no observed change with the oxidative stress condition. Therefore, cat epididymal sperm stored at 5°C appear to maintain a high quality for up to 48 hr of cooling time.  相似文献   

A fast,low‐cost and efficient method for the diagnosis of sperm DNA fragmentation in several species          下载免费PDF全文

BR Rui  DSR Angrimani  LC Bicudo  JDA Losano  M Nichi  RJG Pereira 《Reproduction in domestic animals》2018,53(1):171-175

Sperm DNA fragmentation is a condition that interferes directly in the reproductive efficiency. Currently, there are several methods for assessing the sperm DNA integrity, such as Alkaline Comet, TUNEL and Sperm Chromatin Structure Assay. However, many of these techniques are laborious and require high‐precision equipment. Thus, the development of new techniques can optimize the evaluation of sperm DNA damage. Therefore, the aim of this study was to standardize the toluidine blue (TB) stain technique for the analysis of DNA fragmentation of dog, cat, bull, stallion and ram spermatozoa. For this purpose, we used six animals of each specie (n = 30), in reproductive age. Sperm was collected by different methods according to the particularities of each species, and such samples were divided into two aliquots: a sperm sample was kept at 5°C (considered as intact sperm DNA), and the remaining samples were submitted to the induction of DNA fragmentation by exposure to ultraviolet light for 4 hr. Samples were then mixed with the intact sample to obtain known and progressive proportions of sperm with fragmented DNA (0%, 25%, 50%, 75% and 100%). Semen smears were performed and subjected to staining with TB. Blue‐stained spermatozoa were considered to have DNA fragmentation. We observed high linear regression coefficients between the expected proportion of damaged DNA and the results of TB for dog, cat, ram, bull and stallion samples. In conclusion, TB stain was considered a fast and effective technique for the study of spermatozoa DNA in several species.  相似文献   

Equine Surgery          下载免费PDF全文

Bradley R. Jackman 《The Canadian veterinary journal. La revue veterinaire canadienne》1994,35(5):305

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Use of a three-drill-tract technique for arthrodesis of the distal tarsal joints in horses with distal tarsal osteoarthritis: 54 cases (1990-1999)     

Dechant JE  Baxter GM  Southwood LL  Crawford WH  Jackman BR  Stashak TS  Trotter GW  Hendrickson DA 《Journal of the American Veterinary Medical Association》2003,223(12):1800-1805

OBJECTIVE: To assess the long-term clinical outcome of horses with distal tarsal osteoarthritis (OA) in which a 3-drill-tract technique was used to induce arthrodesis of the affected joints, identify any preoperative or operative factors associated with outcome, and describe any complications associated with the technique. DESIGN: Retrospective study. ANIMALS: 54 horses. PROCEDURE: Medical records were reviewed for information on signalment, use, history, physical and lameness examination findings, surgical technique, and postoperative care. Radiographs were examined, and severity of OA was graded. Follow-up information was obtained through telephone interviews with owners at least 13 months after the procedure. RESULTS: 32 (59%) horses had a successful outcome, 6 (11%) improved but were not sound after surgery, and 16 (30%) did not improve following surgery. Outcome was negatively associated with the previous use of intra-articular injections. Few postoperative complications were evident. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that distal tarsal OA in horses can be successfully treated by means of distal tarsal arthrodesis with a 3-drill-tract technique. Horses with advanced distal tarsal OA are likely to have poorer outcomes, and the procedure will likely be of minimal benefit in horses with concomitant causes of hind limb lameness prior to surgery and in horses with preexisting proximal intertarsal joint disease.  相似文献   

Sectional notes     

E. Marx  A. H. Eddins  G. D. Ruehle  H. L. Cochran  A. C. Moreau  J. V. Pace  Marx Koehnke  J. C. Campbell  M. E. Gardner  E. R. Jackman  C. A. Henderson  K. W. Lauer  C. N. Clayton  John Noonan  D. H. Friend  G. S. Ralston  H. M. Darling 《American Journal of Potato Research》1943,20(4):95-106

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Sectional notes     

E. Marx  C. H. Metzger  B. A. Brown  Eugene W. Whitman  H. R. Talmage  Frank W. Hussey  H. A. Reiley  Marx Koehnke  J. C. Campbell  E. V. Hardenburg  Karl H. Fernow  Earl B. Tussing  E. R. Jackman  C. A. Henderson  O. D. Burke  K. W. Lauer  John Noonan  H. L. Bailey  Chas D. Gaines  W. N. Keenan  R. E. Goodin 《American Journal of Potato Research》1943,20(8):224-236

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Sectional notes     

H. W. Poulsen  H. G. Zuckerman  C. H. Metzger  W. B. Ward  W. C. Edmundson  E. R. Bennet  R. A. Jehle  A. B. Beaumont  John S. Gardner  E. L. Newdick  Frank W. Hussey  E. J. Wheeler  H. C. Moore  Marx Koehnke  A. G. Tolaas  E. E. Isaac  H. O. Werner  O. Butler  William H. Martin  K. H. Fernow  E. R. Jackman  E. B. Tussing  E. V. Hardenburg  J. B. R. Dickey  K. H. Klages  G. S. Ralston  H. L. Bailey  John Tucker 《American Journal of Potato Research》1934,11(8):207-226

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Sectional notes     

M. A. Lindsay  E. R. Jackman  John Noonan  John L. Toevs  W. B. Ward  H. A. Reiley  J. C. Campbell  E. V. Hardenburg  C. A. Henderson 《American Journal of Potato Research》1945,22(11):350-354

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Sectional notes     

H. G. Zuckerman  C. H. Metzger  B. A. Brown  W. B. Ward  Frank W. Hussey  R. W. Donaldson  E. E. Isaac  Marx Koehnke  William H. Martin  K. H. Fernow  E. V. Hardenburg  Robert Schmidt  E. M. Gillig  E. B. Tussing  E. R. Jackman  T. E. Odland  S. P. Swenson  John Tucker 《American Journal of Potato Research》1936,13(8):223-232

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Sectional notes     

M. A. Lindsay  C. H. Metzger  John R. Robertson  W. B. Ward  Verne C. Beverly  E. R. Jackman  John Noonan  H. R. Talmage 《American Journal of Potato Research》1946,23(8):304-312

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