Insulin dysregulation in horses with systemic inflammatory response syndrome          下载免费PDF全文

François‐René Bertin  Debra Ruffin‐Taylor  Allison Jean Stewart 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(4):1420-1427

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Genetic diversity and population structure of anciently introduced Cuban cacao Theobroma cacao plants     

Igor Bidot Martínez  Manuel Riera Nelson  Marie-Christine Flamand  Pierre Bertin 《Genetic Resources and Crop Evolution》2015,62(1):67-84

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Effect of sirolimus on insulin dynamics in horses     

Demia J. de Tonnerre  Carlos E. Medina Torres  Darko Stefanovski  Mary A. Robinson  Kate L. Kemp  François-René Bertin  Andrew W. van Eps 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(2):703-712

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Effects of acute asphyxia at birth on subsequent heat production capacity in newborn pigs     

Herpin P  Wosiak F  Le Dividich J  Bertin R 《Research in veterinary science》1999,66(1):45-49

The effect of acute asphyxia at birth on subsequent ability to produce heat was investigated in 30 newborn pigs. A model of experimentally induced asphyxia consisting of the prevention of breathing within the first four minutes of life was used. Blood was sampled from an umbilical artery catheter within the first 75 minutes of life for blood gas, pH, glucose, lactate and catecholamine analysis. After the treatment and 24 hours later, heat production capacity, shivering intensity and rectal temperature were measured 10 degrees C below thermoneutrality. Effects on blood gas parameters were severe but transient whereas alterations in carbohydrate metabolism were maintained during the first 75 minutes (P < 0.05). Acute asphyxia at birth induced only minor alterations of thermoregulatory abilities during the first day of life: rectal temperature was lower one hour after birth (P < 0.05) and the postnatal increase in heat production capacity was less pronounced than in controls. It is suggested that the lower viability usually reported for piglets suffering from asphyxia during delivery is most likely to result from reduced vigour and colostrum intake, as well as altered carbohydrate metabolism early after birth.  相似文献   

Susceptibility of Chinese Meishan and European large white pigs to enterotoxigenic Escherichia coli strains bearing colonization factor K88, 987P, K99, or F41   总被引：1，自引：0，他引：1  

M F Duchet-Suchaux  A M Bertin  P S Menanteau 《American journal of veterinary research》1991,52(1):40-44

Conventionally raised Chinese Meishan and European Large White pigs were intragastrically challenge exposed with 2.1 x 10(10) enterotoxigenic Escherichia coli strains bearing colonization factor K88, 987P, F41, or F41 plus K99. In response to challenge exposure with the K88-positive (K88+) organisms, 96% of Large White pigs died within 48 hours, whereas none of the Meishan pigs died. Both breeds of pigs had similar susceptibility to strains bearing 987P or F41. Lastly, Meishan pigs were found to be more susceptible than Large White pigs to a strain expressing K99 and F41. In pigs with diarrhea, challenge-exposure strains intensively colonized the jejunum (10(8) to 10(10) bacteria/g of tissue) and, to less extent, the duodenum (except K88+ strain, which comprised 10(8)/g). In most cases, jejunal concentrations of the challenge-exposure strains were substantially lower in pigs that did not have diarrhea. Half the resistant Meishan pigs eliminated the K88+ strain from the intestines. Colostral antibody titer that agglutinated challenge-exposure strains did not differ between Meishan and Large White gilts. Results indicate that resistance of pigs to the K88+ strain did not extend to enterotoxigenic strains bearing other well-known factors. They indicate, in addition, that genetic resistance to K88+ strains described in pigs in Europe may exist in pigs in China.  相似文献   

Somaclonal variation and improvement in chilling tolerance in rice: changes in chilling-induced electrolyte leakage   总被引：2，自引：0，他引：2  

P. Bertin    J. Bouharmont  J.-M. Kinet 《Plant Breeding》1996,115(4):268-272

Excised leaves of four rice varieties, ‘Facagro 57’, ‘Facagro 76’, ‘Kirundo 3’, ‘Kirundo 9’, were chilled for six days at 10/5°C day/night, along with 11 somaclonal families arising from three of these varieties, which had been selected over four generations for improved chilling tolerance. At the end of the chilling treatment, electrolyte leakage from leaf pieces was followed for 12 h and compared with an unchilled control. With unchilled leaves, no varietal differences nor a rise in leakage over time were observed. Chilling induced higher leakages in all varieties, except in ‘Kirundo 3’ at the time of first measurement, and a continuous rise over time was found in all varieties. Varietal differences were detected, ‘Kirundo 3’ being the least affected, followed by ‘Kirundo 9’ and ‘Facagro 76’, and finally ‘Facagro 57’, which coincides with their ranking for chilling tolerance established through chilling survival tests. The leakages were generally lower in the somaclonal families than in the variety they originated from in ‘Kirundo 9’ and ‘Facagro 76’, while the opposite was observed in ‘Kirundo 3’. The significance and usefulness of electrolyte leakage measurement for chilling tolerance screening, and the potentials of somaclonal variation for chilling tolerance improvement are discussed.  相似文献   

A map of the interactome network of the metazoan C. elegans   总被引：1，自引：0，他引：1  

Li S  Armstrong CM  Bertin N  Ge H  Milstein S  Boxem M  Vidalain PO  Han JD  Chesneau A  Hao T  Goldberg DS  Li N  Martinez M  Rual JF  Lamesch P  Xu L  Tewari M  Wong SL  Zhang LV  Berriz GF  Jacotot L  Vaglio P  Reboul J  Hirozane-Kishikawa T  Li Q  Gabel HW  Elewa A  Baumgartner B  Rose DJ  Yu H  Bosak S  Sequerra R  Fraser A  Mango SE  Saxton WM  Strome S  Van Den Heuvel S  Piano F  Vandenhaute J  Sardet C  Gerstein M  Doucette-Stamm L  Gunsalus KC  Harper JW  Cusick ME  Roth FP  Hill DE  Vidal M 《Science (New York, N.Y.)》2004,303(5657):540-543

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干旱胁迫对苗木蒸腾耗水日变化的影响   总被引：29，自引：4，他引：29  

招礼军  李吉跃  于界芬  Sophie Bertin 《北京林业大学学报》2003,25(3):42-47

采用BP340 0精密电子天平 ,于 2 0 0 2年夏季研究了在不同水分胁迫条件下 ,4种北方主要造林树种苗木的日蒸腾耗水速率及实际耗水量变化规律 .结果表明 ,在正常水分条件下 ,各苗木的日最大耗水速率出现在 10 :0 0— 14 :0 0之间 ,阔叶树种的蒸腾耗水速率远远大于针叶树种 ,是针叶树种的 5～ 7倍 ,苗木的耗水速率排序为 :黄栌 >火炬树 >侧柏 >油松 ;1年生的黄栌和火炬树与 5年生侧柏的日耗水量相差不大 ,是油松的 1倍 .当苗木受到干旱胁迫后 ,苗木的日最大耗水速率会提前 ,蒸腾速率迅速下降 ,但下降幅度不同 ,耗水速率排序为 :火炬树 >黄栌 >油松 >侧柏 .在中等干旱胁迫下 ,油松、火炬树、侧柏、黄栌的日平均耗水速率分别下降了 5 4 0 %、6 8 6 %、87 2 %和 90 2 % ;侧柏和黄栌之间 ,油松和火炬树之间的日耗水量基本相同 ,但侧柏和黄栌只有油松和火炬树的一半 ;干旱胁迫继续加重后 ,油松、火炬树、侧柏、黄栌的日平均耗水速率只有水分正常条件下的 15 7%、12 1%、4 3%和 9 2 % ,日耗水总量下降到 5 %～10 % ,4个树种间相差不大 .  相似文献   

Relationship between virulence and adherence of various enterotoxigenic Escherichia coli strains to isolated intestinal epithelial cells from Chinese Meishan and European large white pigs   总被引：6，自引：0，他引：6  

A M Bertin  M F Duchet-Suchaux 《American journal of veterinary research》1991,52(1):45-49

In vitro adherence to intestinal epithelial cells by enterotoxigenic Escherichia coli strains bearing K88, K99, F41, or 987P adhesins and of their variants not bearing adhesins (K88-, K99-, or F41-) was investigated in European Large White and Chinese Meishan pigs. Possible relationship between adherence and virulence was also examined. The K88-positive (K88+) strain strongly adhered to intestinal epithelial cells from 26 of 28 Large White pigs. This strain had previously been found to be highly virulent for Large White pigs. The only surviving pig was of nonadherent phenotype, and cells from 4 dehydrated moribund pigs had strong adherence. By contrast, the same K88+ strain found previously to have little pathogenicity for Meishan pigs adhered with variable intensity to cells from 17 of 23 Meishan pigs; correlation was not evident between adherence and virulence. The K99+ F41+ strain of porcine origin and the F41+ strain generally adhered strongly to cells from 24 and 23 Meishan pigs, respectively, and to cells from 25 of 26 Large White pigs. Correlation was not found between adherence and virulence for the 2 strains. A K99+ F41+ strain of bovine origin adhered to cells from 20 of 22 Meishan and 22 of 23 Large White pigs, and a K99- F41+ variant adhered to cells from 19 of 23 Meishan and 23 of 24 Large White pigs. The adhesin-negative variants never adhered to intestinal epithelial cells. Strain 987 known not to readily produce 987P adhesin after in vitro growth never adhered to cells during the test.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Note: A comparison of molecular diagnostic procedures for the detection of aster yellows phytoplasmas (16Sr-I) in leafhopper vectors     

Sabrina?Bertin  Simona?Palermo  Cristina?Marzachì  Domenico?BoscoEmail author 《Phytoparasitica》2004,32(2):141-145

Different molecular procedures were compared for the detection of aster yellows phytoplasmas (AYP) in the leafhopper vectorsMacrosteles quadripunctulatus (Kirschbaum),Euscelidius variegatus (Kirschbaum) andEuscelis incisus (Kirschbaum). Polymerase chain reaction (PCR) with universal and group-specific primers designed on the 16S-rDNA sequence was most sensitive in nested assays. A dot-blot procedure with an oligoprobe designed on the 16S-rDNA was less sensitive and consistent to detect phytoplasmas in total insect DNA, but consistently detected amplicons from direct PCR. The dot-blot assay with a probe based on a phytoplasma plasmid sequence detected AYP in most vector specimens and did not react with DNAs from leafhoppers infected by flavescence dorée and psyllids infected by apple proliferation phytoplasmas. This last assay is almost devoid of contamination risks, faster and cheaper compared to PCR, therefore it has to be preferred for field-scale analysis of leafhopper populations. http://www.phytoparasitica.org posting Feb. 24, 2004.  相似文献