SIR FREDERICK BANTING     

Best CH 《Science (New York, N.Y.)》1941,93(2411):248-249

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Survey of Equine Referring Veterinarians' Satisfaction with Their Most Recent Equine Referral Experience          下载免费PDF全文

C. Best  J.B. Coe  J. Hewson  M. Meehan  D. Kelton 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(2):822-831

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Cloning,expression, purification,and biological activity of five feline type I interferons   总被引：1，自引：0，他引：1  

Wonderling R  Powell T  Baldwin S  Morales T  Snyder S  Keiser K  Hunter S  Best E  McDermott MJ  Milhausen M 《Veterinary immunology and immunopathology》2002,89(1-2):13-27

Type I interferons (IFN) are important mediators of the host defense against viral infections in mammals. In humans multiple subtypes of IFN-alpha exist, most of which possess antiviral activity. Little is known about the type I IFN genes in cats and the role they may play in feline immunological responses to viruses. We have isolated cDNAs encoding five feline IFN-alpha (feIFN) subtypes that share from 95 to 99% amino acid sequence identity. FeIFN-alpha5 has five additional amino acids inserted at position 139, which are not present in the other four subtypes. Sequence identity of the feIFN proteins encoded by the five clones compared to human IFN-alpha2 is approximately 60%. Unlike most of the human subtypes, each of the five feline IFN sequences has an N-glycosylation recognition site. Expression of all five feIFN-alpha subtypes in Chinese hamster ovary (CHO) cells was confirmed by Western blot analysis, and all resulting proteins were glycosylated. The antiviral activity of each feIFN-alpha subtype produced in transiently transfected CHO cell cultures was tested in vitro. In addition, subtype feIFN-alpha6 was expressed in the yeast, Pichia pastoris. The resulting secreted mature recombinant protein was purified and demonstrated significant antiviral activity and induction of 2',5'-oligoadenylate synthetase activity in vitro.  相似文献   

THE SIGNIFICANCE OF CHOLINE AS A DIETARY FACTOR     

Best CH 《Science (New York, N.Y.)》1941,94(2449):523-527

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Displacement of diquat from clay and its phytotoxicity     

Weber JB  Weed SB  Best JA 《Journal of agricultural and food chemistry》1969,17(5):1075-1076

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Tracing ingestion of 'novel' foods in UK diets for possible health surveillance--a feasibility study     

Robertson C  Best N  Diamond J  Elliott P 《Public health nutrition》2004,7(2):345-352

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The use of trienbolone acetate implants in heifer beef production at pasture     

J M Best 《The Veterinary record》1972,91(25):624-626

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Failure to detect transmission of Escherichia coli O157:H7 from orally dosed nannies to sucking kids at foot     

La Ragione RM  Ahmed NM  Best A  Clifford D  Weyer U  Woodward MJ 《The Veterinary record》2005,157(21):659-661

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Century One, a History of the Ontario Veterinary Association, 1874-1974          下载免费PDF全文

R. H. Best 《The Canadian veterinary journal. La revue veterinaire canadienne》1977,18(11):324

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Escherichia coli O115 forms fewer attaching and effacing lesions in the ovine colon in the presence of E. coli O157:H7     

La Ragione RM  Best A  Aktan I  Clifton-Hadley FA  Dugdale AH  Weyer U  Crawley T  Townsend P  Cooley WA  Johnson L  Wangoo A  Woodward MJ  Wales AD 《Research in veterinary science》2012,93(1):42-45

Escherichia coli O115 has been isolated from healthy sheep and was shown to be associated with attaching-effacing (AE) lesions in the large intestine. Following previous observations of interactions between E. coli O157 and O26, the aim of the present study was to assess what influence an O115 AE E. coli (AEEC) would have on E. coli O157 colonisation in vitro and in vivo. We report that E. coli O115- and O157-associated AE lesions were observed on HEp-2 cells and on the mucosa of ligated ovine spiral colon. In single strain inoculum, E. coli O115 associated intimately with HEp-2 cells and the spiral colon in greater numbers than E. coli O157:H7. However, in mixed inoculum studies, the number of E. coli O115 AE lesions was significantly reduced suggesting negative interference by E. coli O157. Use of the ligated colon model in the present work has allowed in vitro observations to be extended and confirmed whilst using a minimum of experimental animals. The findings support a hypothesis that some AEEC can inhibit adhesion of other AEEC in vivo. The mechanisms involved may prove to be of utility in the control of AE pathovars.  相似文献