PST脂质体在猪血浆中稳定性的研究     

胥传来  乐国伟  姚惠源 《四川畜牧兽医》2002,29(2):21-22

本文旨在通过逆相蒸发法制备PST脂质体，通过测定酸价，丙二醛含量的变化，并做了猪血浆体外24h实验，验证了PST脂质体在血浆中能稳定存在，为PST脂质体的临床应用作初步的研究。  相似文献   

Forms of employment and species caseload of veterinary practitioners in New Zealand     

Boland CJ  Morris RS 《New Zealand veterinary journal》1989,37(3):112-116

A survey by means of a postal questionnaire was undertaken to investigate the patterns of work and the need for information of veterinary practitioners in New Zealand. Of the 670 eligible veterinarians, 399 practitioners (60 per cent) participated in the survey. Of these, 38 per cent were in large animal practice (less than 20 per cent of work devoted to cats and dogs) and 31 per cent were in small animal practice (more than 80 per cent of work with cats and dogs). The remaining 31 per cent were in mixed practice, with a workload intermediate between the other two groups. Across the entire sample of practitioners, cats and dogs took up the largest number of veterinary hours per person (1092 hours per year). Dairy cattle were second (438 hours), and horses third (302 hours). Deer and goats ranked next, and each used more veterinary hours per person than did either sheep or beef cattle. Other species comprised very minor parts of the overall workload. Women spent a much higher proportion of their working hours with small animals and a much lower proportion with horses than did men. For other species workload patterns were similar between men and women. In relation to employment of the practitioner group, women were under represented, compared with men, among those with responsibilities for the management of practices, even when account was taken of the fact that the women in the sample were younger. Fewer than one per cent of men in the sample were not employed full-time, whereas 15 per cent of the women were in part-time employment. The survey indicates that there has been a substantial change in the demographic structure of the veterinary profession and the forms of veterinary work carried out. It also shows that the differences in work and career patterns between men and women need more intensive study to improve the accuracy of predictions of future requirements for veterinary manpower.  相似文献   

差异显示法研究不同锌源对组织基因表达的影响     

虞泽鹏  乐国伟  施用晖 《畜牧与兽医》2004,36(4):4-6

选用Avine肉用仔鸡作为试验对象 ,饲喂硫酸锌和蛋氨酸锌 2种锌源。采用银染差异显示技术进行差异基因的初步筛选。结果成功回收扩增差异条带 2 6条 ;其中加锌组 (硫酸锌组和蛋氨酸锌组 ) 2 3条 ,3条为缺锌组所特有。在加锌组的 2 3条中 ,6条为蛋氨酸锌组所特有 ,1 3条为硫酸锌组所特有 ,其余 4条为 2组所共有。这些初步筛选得到的差异基因尚需进一步进行克隆、测序和杂交进行验证。  相似文献   

昆虫抗菌肽生物学活性及其应用前景   总被引：8，自引：0，他引：8  

宫霞  施用晖  乐国伟 《中国畜牧杂志》2004,40(8):37-40

昆虫抗菌肽是昆虫免疫后存在于血淋巴中的一类活性肽 ,具有广谱的抗菌、抗病毒、抑制肿瘤的生物活性 ,具有很高的应用潜力。本文主要介绍昆虫抗菌肽的类型、生理活性、基因的克隆与表达及在动物科学中的应用前景  相似文献   

不同锌源对断奶小鼠生长及机体抗氧化能力的影响   总被引：9，自引：1，他引：9  

虞泽鹏  乐国伟  施用晖  侯丽 《畜牧与兽医》2005,37(4):1-3

选用断奶小鼠72只作为研究对象,随机分为3组(对照组,硫酸锌组和蛋氨酸锌组),每组设4个重复。测定体重、组织锌含量及相关生理生化指标,以研究硫酸锌、蛋氨酸锌2种不同锌供给形式对机体的生长效应及抗氧化能力的影响。结果表明,硫酸锌组和蛋氨酸锌组均能不同程度地提高小鼠体重,蛋氨酸锌组小鼠体重显著高于对照组与硫酸锌组(P<0.05)。饲粮中添加硫酸锌可提高小鼠肝脏和血清中的锌含量(P>0.05);蛋氨酸锌组小鼠肝脏和血清锌含量高于对照组(P<0.05)及硫酸锌组(P>0.05)。2种锌源均显著提高碱性磷酸酶(AKP)、谷胱甘肽过氧化物酶(GSHPx)活性(P<0.05),不同锌源间无明显差异(P>0.05)。添加不同的锌源不同程度地提高总抗氧化能力(TAOC)、总SOD(TSOD)、铜锌超氧化物歧化酶(CuZnSOD)活性(P<0.05),蛋氨酸锌组TAOC、TSOD显著高于硫酸锌组(P<0.05)。添加锌显著降低NO含量(P<0.05),蛋氨酸锌效果显著强于硫酸锌(P<0.05);一氧化氮合酶(NOS)活性表现出与NO相反的趋势(P>0.05)。  相似文献   

植物TIR1/AFBs基因家族研究进展          下载免费PDF全文

滕青云  乐丽娜  宋冰倩  陈英 《农业工程》2020,10(2):93-97

TIR1/AFBs基因家族是一种存在于细胞核中的生长素受体,属于F-box蛋白基因中的一个小亚族。它们通过与相关生长素相结合活化转录因子来促进基因的表达,从而进行调控,是生长素信号转导过程中的关键部分。为了深入研究生长素信号转导机制,从TIR1/AFBs基因家族的发现与结构,家族成员表达模式的差异及对植物生长发育方面的调节等方面概括介绍了TIR1/AFBs基因家族的分子调控机制,总结了TIR1/AFBs基因的功能。最后探讨了TIR1/AFBs基因的研究方向。  相似文献   

氮添加对天山高寒草原土壤酶活性和酶化学计量特征的影响   总被引：1，自引：0，他引：1  

勒佳佳  苏原  彭庆文  耿凤展  韩文轩  李文利  李凯辉  刘学军 《干旱区研究》2020,(2):382-389

为探究氮添加对高寒草原生态系统土壤酶活性的影响,于2018年在中国科学院巴音布鲁克草原生态系统研究站,选择4个氮添加水平(对照,N0,0 kg·hm^-2·a^-1;低氮,N1,10 kg·hm^-2·a^-1;中氮,N3,30 kg·hm^-2·a^-1;高氮,N9,90 kg·hm^-2·a^-1),开展土壤酶活性对氮添加响应的研究,分析土壤酶活性对氮添加的响应特点,土壤酶化学计量比以及土壤酶活性与土壤环境因子的关系。结果表明:与对照相比,氮添加在N3水平显著增加β-1,4葡萄糖苷酶(βG)、β-D-纤维二糖水解酶(CBH)和β-1,4木糖苷酶(βX)酶活性(P<0.05),N1和N3水平显著增加碱性磷酸酶(AKP)活性(P<0.05),N3水平显著降低多酚氧化酶(PPO)活性(P<0.05),氮添加对亮氨酸氨基肽酶(LAP)活性影响不显著,N3水平下显著增加N-乙酰-β-D氨基葡萄糖苷酶(NAG)活性(P<0.05)。相关分析表明,8种土壤酶活性均与土壤有机碳(SOC、NAG除外)和总磷(TP)显著相关,与土壤总氮(TN)不相关。研究区土壤酶活性C∶N∶P化学计量比为1∶1∶1.2,与全球生态系统的土壤酶活性C∶N∶P的比值1∶1∶1相偏离,表明该研究区土壤微生物生长受磷素限制。冗余分析(RDA)进一步揭示出土壤有机碳和土壤全磷含量是影响土壤酶活性的主要因子。  相似文献   

Socio-spatial organization reveals paternity and low kinship in the Honduran white bat (Ectophylla alba) in Costa Rica     

Edgar G. GUTIÉRREZ  Isabela VIVAS-TORO  Daniela CARMONA-RUÍZ  David VILLALOBOS-CHAVES  Bernal RODRÍGUEZ-HERRERA  Melina Del REAL-MONROY  Gloria LEÓN-AVILA  Jorge ORTEGA 《Integrative zoology》2021,16(5):646-658

Ectophylla alba is a tent-making bat that roosts in mixed-sex clusters comprising adults and offspring. Our goal was to determine the genetic identity of individuals belonging to different roosting groups. We tested the hypothesis of kin selection as a major force structuring group composition. We used 9 microsatellites designed for E. alba to determine the genetic identity and probability of parentage of individuals. We analyzed parentage and kinship using the software ML-Relate, GenAIEx, and Cervus. The obtained relationship probabilities (0.5) revealed a clear maternal relationship between female adults and offspring with allele compatibility, and at least 5 relationships between male adults and pups. We found a low degree of relatedness within roosting groups. Between roosting groups at different sites, the mean probability of a half-sibling relationship ranged from 0.214 to 0.244 and, for full-sibling relationship, from 0.383 to 0.553. Genetically, adult individuals were poorly related within clusters, and kinship as an evolutionary force could not explain group membership.  相似文献   

物性分析仪测定鸭梨质构条件的优化     

徐金涛  高丽娟  李龙飞  张海娥  乐文全  郝宝锋 《北方园艺》2016,(18):29-33

以鸭梨果实为试材,采用物性分析仪TPA模式在不同压缩速度和目标形变量下,测定鸭梨果实的质地参数,以优化TPA模式评价的测定条件。结果表明:在TPA测定过程中,目标形变量对硬度、内聚性、弹性、粘附性、胶着性、恢复性、断裂性和咀嚼性等8个TPA参数的影响极显著(P≤0.01);而压缩速度对硬度和胶着性性影响显著(P≤0.05),对断裂性极显著(P≤0.01),对其它5个TPA参数影响不显著。选用1.0mm·s-1的压缩速度和20%的目标形变量作为鸭梨果实TPA模式下的测定条件,能够客观地反映鸭梨果肉组织的质地特征。  相似文献   

玉米生长后期的根系分布研究          下载免费PDF全文

廖荣伟  刘晶淼  白月明  安顺清  梁宏  卢建立  乐章燕  曹玉静 《中国生态农业学报》2014,22(3):284-291

为了研究玉米生长后期根系的生长发育规律,利用中国气象局固城农业气象试验站大型根剖面系统,采用微根管观测系统及方形整段标本法和地下根系室玻璃窗,对‘屯玉46号’玉米根系的生长状况进行了试验研究。结果表明:垂直方向上,方形整段标本法和微根管法测得的根长密度占整层总根长密度比例的变化趋势一致,相关系数分别为0.987和0.717,且两种方法在0~20 cm土层的根长密度比例均为最大。0~60 cm土层为玉米根系生长活跃区,方形整段标本法测得根长密度生长量为其余层的4倍。两种方法测得的根长密度无显著差异,相关系数为0.830,均匀性水平较好。玉米成熟期根系的水平幅度较乳熟期窄,下层根系仍处于生长中,垂直深度增加。玻璃窗与方形整段标本法观测的根深测定结果存在差异,这可能与观测环境条件不一致有关。  相似文献