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Bolormaa S Neto LR Zhang YD Bunch RJ Harrison BE Goddard ME Barendse W 《Journal of animal science》2011,89(8):2297-2309
Chromosomal regions containing DNA variation affecting the traits intramuscular fat percentage (IMF), meat tenderness measured as peak force to shear the LM (LLPF), and rump fat measured at the sacro-iliac crest in the chiller (CHILLP8) were identified using a set of 53,798 SNP genotyped on 940 taurine and indicine cattle sampled from a large progeny test experiment. Of these SNP, 87, 64, and 63 were significantly (P < 0.001) associated with the traits IMF, LLPF, and CHILLP8, respectively. A second, nonoverlapping sample of 1,338 taurine and indicine cattle from the same large progeny test experiment genotyped for 335 SNP, including as a positive control the calpastatin (CAST) c.2832A > G SNP, was used to confirm these locations. In total, 37 SNP were significantly (P < 0.05) associated with the same trait and with the same favorable homozygote in both data sets, representing 27 chromosomal regions. For the trait IMF, the effect of SNP in the confirmation data set was predicted from the discovery set by multiplying the estimated allele effect of each SNP in the discovery set by the number of copies of the reference allele of each SNP in the confirmation set. These weighted effects were then summed over all SNP to generate a molecular breeding value (MBV) for each animal in the confirmation data set. Using a bivariate analysis of MBV and IMF phenotypes of animals in the confirmation set, a panel of 14 SNP explained 5.6 and 15.6% of the phenotypic and genetic variance of IMF, respectively, in the confirmation data set. The amount of variation did not increase as more SNP were added to the MBV and instead decreased to 1.2 and 3.8% of the phenotypic and genetic variance of IMF, respectively, when 329 SNP were included in the analysis. 相似文献
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M. Watanabe Y. Inoue N. Sakagami O. Bolormaa K. Kawasaki S. Hiradate N. Fujitake & H. Ohta 《European Journal of Soil Science》2007,58(3):786-793
Sclerotium grains in soil contain humus-metal complexes that are probably produced from fungal metabolites. The characterization of major elements in sclerotium grains collected from volcanic ash soils in Mt Myoko was examined by X-ray photoelectron spectroscopy, FT-IR spectrometry and CHN analysis, and the concentration of trace elements was determined by PIXE (particle induced X-ray emission spectrometer) analysis. The content of major elements, C, H, N, O and Al, was approximately 47.6, 3.32, 0.78, 30.2 and 1.4% by mass, respectively. Trace elements such as Ti, Cr, Mn, Cu, Zn, Br and Pb were detected in the grains at concentrations between 10 and 100 μg g−1 . Functional carbon groups for the whole grain were characterized by the dominance of O-alkyl C associated with aromatic C. The comparison between the surface and subsurface (matrix) of the grain showed that the concentrations of O, C and N were relatively greater on the surface of sclerotium grains than in the matrix. The proportion of carbon having C–O, C=O, and O–C=O bonds, O and N showed a tendency to decrease from the surface towards the matrix. The proportion of C assigned as C–C and/or C–H bonds had a tendency to increase towards the matrix associated with Al. 相似文献
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Bolormaa S Hayes BJ Hawken RJ Zhang Y Reverter A Goddard ME 《Journal of animal science》2011,89(7):2050-2060
Cattle in breeds formed by recent crossing of Bos taurus (Bt) and Bos indicus (Bi) subspecies should contain chromosomes that are a composite of Bt and Bt segments. Using data from a 50K SNP chip, we were able to identify whether a chromosome segment of 11 SNP in a composite animal descended from a Bt or a Bi ancestor. When the method was tested in purebred Bt or Brahman cattle, about 94% of segments were assigned correctly. About 10% of the genome in Australian Brahman cattle appears to be of Bt origin, as might be expected from their history. We then examined the effect of the origin of each chromosome segment on BW in a population of 515 Bt × Bi composite cattle and found 67 chromosome segments with a significant (P<0.01) effect. We confirmed these effects by examining these 67 segments in a population of Brahman cattle and in a population of mixed breeds including composite breeds such as Santa Gertrudis and Brahman cattle. About 66% of the 67 segments had an effect in the same direction in the confirmation analyses as in the discovery population. However, the effect on BW and other traits of chromosome segment origin is small, indicating that we had low power to detect these effects with the number of animals available. Consequently, when chromosome segment origin was used in genomic selection to predict BW, the accuracy was low (0.08). Chromosome segments that had a positive effect on BW tend to be at greater frequency in composite breeds than chromosome segments with a negative effect on BW. 相似文献
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S. Bolormaa J.H.J. Van Der Werf S.W. Walkden‐Brown K Marshall A. Ruvinsky 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2010,127(3):207-214
Three microsatellite markers on goat chromosome 23 adjacent to the MHC were used to test for quantitative trait loci (QTL) affecting faecal worm egg count (WEC) and leukocyte traits in ten Australian Angora and twelve Australian Cashmere half‐sib families (n = 16–57 per family). Data were collected from 280 Angora and 347 Cashmere kids over a 3‐ and 4‐year period. A putative QTL affecting trichostrongyle WEC was found in two small families at the 5% chromosome‐wise threshold level. The biggest QTL effect for WEC of 1.65 standard deviations (σp) was found within the region of OarCP73‐BM1258. A significant QTL affecting blood eosinophil counts at the 1% chromosome–wise threshold level was detected at marker BM1258 (at 26 cM) in two Angora and Cashmere families. The magnitude of the putative QTL was 0.69 and 0.85 σp in Angora and Cashmere families, respectively. Due to the comparatively low power of the study these findings should be viewed as indicative rather than definitive. 相似文献
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Bolormaa S Hayes BJ Savin K Hawken R Barendse W Arthur PF Herd RM Goddard ME 《Journal of animal science》2011,89(6):1684-1697
A genome wide-association study for production traits in cattle was carried out using genotype data from the 10K Affymetrix (Santa Clara, CA) and the 50K Illumina (San Diego, CA) SNP chips. The results for residual feed intake (RFI), BW, and hip height in 3 beef breed types (Bos indicus, Bos taurus, and B. indicus × B. taurus), and for stature in dairy cattle, are presented. The aims were to discover SNP associated with all traits studied, but especially RFI, and further to test the consistency of SNP effects across different cattle populations and breed types. The data were analyzed within data sets and within breed types by using a mixed model and fitting 1 SNP at a time. In each case, the number of significant SNP was more than expected by chance alone. A total of 75 SNP from the reference population with 50K chip data were significant (P < 0.001) for RFI, with a false discovery rate of 68%. These 75 SNP were mapped on 24 different BTA. Of the 75 SNP, the 9 most significant SNP were detected on BTA 3, 5, 7, and 8, with P ≤ 6.0 × 10(-5). In a population of Angus cattle divergently selected for high and low RFI and 10K chip data, 111 SNP were significantly (P < 0.001) associated with RFI, with a false discovery rate of 7%. Approximately 103 of these SNP were therefore likely to represent true positives. Because of the small number of SNP common to both the 10K and 50K SNP chips, only 27 SNP were significantly (P < 0.05) associated with RFI in the 2 populations. However, other chromosome regions were found that contained SNP significantly associated with RFI in both data sets, although no SNP within the region showed a consistent effect on RFI. The SNP effects were consistent between data sets only when estimated within the same breed type. 相似文献
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外购干草在牧民载畜行为及生产方式转型中扮演着重要角色.本文利用内蒙古450户的牧户调研数据,通过核减干草效用计算牧户实际载畜率值,探究核减前后牧户载畜率的变化趋势,剖析核减干草后在新一轮草原补奖政策标准下牧户超载情况.结果表明:三类草原区牧户在核减外购干草效用后的载畜率较核减前均有不同程度下降,其中草甸草原区降幅最大,... 相似文献
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Production of a bacteriocin‐like inhibitory substance by Leuconostoc mesenteroides subsp. dextranicum 213M0 isolated from Mongolian fermented mare milk,airag 
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下载免费PDF全文 Kensuke Arakawa Saki Yoshida Hiroki Aikawa Chihiro Hano Tsognemekh Bolormaa Sedkhuu Burenjargal Taku Miyamoto 《Animal Science Journal》2016,87(3):449-456
Strain 213M0 was selected with productivity of a bacteriocin‐like inhibitory substance (BLIS) among 235 strains of lactic acid bacteria (LAB) isolated from Mongolian fermented milk ‘airag’. Strain 213M0 was species‐identified as Leuconostoc mesenteroides subsp. dextranicum by morphological observation, carbohydrate fermentation profiling and sequencing the 16S rRNA gene. Incubation temperature proper to produce the BLIS was 25°C rather than 30 and 37°C, and the production actively proceeded during the exponential growth phase of the producer cells. Antibacterial effect of BLIS 213M0 was limited to all nine strains of Listeria sp. bacteria and seven strains of LAB cocci among 53 tested strains, which corresponds to a typical feature of the class IIa pediocin‐like bacteriocins. BLIS 213M0 was not inactivated in every broad pH range solution (pH 2.0‐11.0), and was stable against storage at 25°C for 1 week and heating at 121°C for 15 min under pH 4.5. Peptide frame of BLIS 213M0 was confirmed by inactivation with some peptidases, and then its molecular weight was estimated to be 2.6‐3.0 kDa using an in situ activity assay following sodium dodecyl sulfate polyacrylamide gel electrophoresis. The estimated size was different from the other Leuconostoc bacteriocins already reported. These results suggest that BLIS 213M0 would be a novel listericidal bacteriocin. 相似文献
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