Effects of recombinant canine granulocyte colony-stimulating factor on white blood cell production in clinically normal and neutropenic dogs.     

L Mishu  G Callahan  Z Allebban  J M Maddux  T C Boone  L M Souza  C D Lothrop 《Journal of the American Veterinary Medical Association》1992,200(12):1957-1964

Recombinant canine granulocyte colony-stimulating factor (rcG-CSF) was administered to clinically normal dogs, cyclic-hematopoietic dogs, and dogs undergoing autologous bone marrow transplantation, to determine whether rcG-CSF could be used to stimulate WBC production and function in normal and neutropenic dogs. To the normal dogs, rcG-CSF was administered by SC injection at rates of 1 microgram/kg of body weight, q 12 h; 2 micrograms/kg, q 12 h; or 5 micrograms/kg, q 12 h. A significant dose-dependent increase in the WBC count resulted from the stimulation of bone marrow progenitor cells. The increased WBC count was characterized by mature neutrophilia and monocytosis. Neutrophil myeloperoxidase and phagocytic activity were normal in rcG-CSF-treated normal dogs, demonstrating the production of normal functional neutrophils in response to rcG-CSF treatment. Recombinant canine G-CSF prevented neutropenia and associated clinical signs but did not completely eliminate the cycling of neutrophils in cyclic-hematopoietic dogs when it was administered at rates of 1 microgram/kg, q 12 h, and 2.5 micrograms/kg, q 12 h. The time to bone marrow reconstitution was not decreased in dogs treated with rcG-CSF at a rate of 2.5 micrograms/kg, q 12 h, for 13 days following autologous bone marrow transplantation. On the basis of our findings, we suggest that treatment with rcG-CSF is an effective way to stimulate myelopoiesis in dogs, but that the dose of rcG-CSF required to stimulate WBC production will vary depending on the cause of neutropenia. Recombinant canine G-CSF should be useful in stimulating production and maintaining function of WBC for treatment of clinical diseases seen commonly in veterinary practice.  相似文献   

Glycine max and Glycine soja are capable of cold acclimation          下载免费PDF全文

J. Robison  N. Arora  Y. Yamasaki  M. Saito  J. Boone  B. Blacklock  S. Randall 《Journal of Agronomy and Crop Science》2017,203(6):553-561

Soybean has been considered a cold intolerant species; based largely upon seed germination and soil emergent evaluations. This study reports a distinct acquisition of cold tolerance, in seedlings, following short acclimation periods. Diversity in cold responses was assessed in eight cultivars of Glycine max and six accessions of G. soja. All varieties of soybean significantly increased in freezing tolerance following acclimation. This study indicates soybean seedlings are indeed capable of sensing cold and acquiring cold tolerance. Germination rates after cold imbibition were negatively correlated with maturity group, but positively correlated with cold acclimation potential in G. soja. Seed fatty acid composition was varied between the species, with Glycine soja accessions containing about 2‐times more linolenic acid (18:3) than G. max. Furthermore, high levels of linoleic acid (18:2) in seeds were positively correlated with germination rates following cold imbibition in G. soja only. We suggest that domestication has not impacted the overall ability of soybean to cold acclimate at the seedling stage and that there is little variation within the domesticated species for ability to cold acclimate. Thus, this brief comparative study reduces the enthusiasm for the “wild” species as an additional source of genetic diversity for cold tolerance.  相似文献   

Equine conidiobolomycosis: A review and case study     

S. Zetterström  R. Hanson  A. Velloso Alvarez  F. Caldwell  L. Boone  J. Schumacher 《Equine Veterinary Education》2021,33(12):e482-e489

Conidiobolus coronatus is one of the most commonly identified upper respiratory fungal pathogens in horses. This article includes a review of clinical signs, diagnostics, treatment and outcomes in previously reported cases of equine conidiobolomycosis, as well as six additional cases seen at our hospital. Each of the six horses presented with a complaint of serosanguinous or haemorrhagic nasal discharge and conidiobolomycosis was confirmed by histopathology and fungal culture. Five horses recovered after administration of antifungal drugs alone (n = 4) or in combination with extensive nasal septum resection (n = 1). Surgical treatment alone was ineffective. One horse was euthanised without treatment because of the extent of the disease.  相似文献   

Reduction and temporary stabilization of acetabular fractures using ASIF mandibular reduction forceps: technique and results using plate fixation in 25 dogs     

Boswell KA  Boone EG  Boudrieau RJ 《Veterinary surgery : VS》2001,30(1):1-10

OBJECTIVE: To report the results of acetabular fracture fixation in 25 dogs in which a specialized forceps (ASIF mandibular reduction forceps, Synthes USA, Paoli, PA) was used to obtain fracture reduction and stabilization. STUDY DESIGN: A retrospective clinical study. ANIMAL POPULATION: Twenty-five client-owned dogs with traumatic acetabular fractures. METHODS: The mandibular reduction forceps (MRF) use a screw on each side of the fracture to attach the clamp directly to the bone and permit direct manipulation of the fragments. Medical records from 25 dogs with acetabular fractures were reviewed to determine the effectiveness of this technique in obtaining, and then maintaining, fracture reduction while a plate was being applied. RESULTS: Clinical results were considered successful in 24 of 25 dogs; the small size of 1 dog prevented application of the MRF. The final reduction and fixation of the fractures was evaluated as anatomic in 17 dogs, near-anatomic in 6 dogs, and nonanatomic in 1 dog. CONCLUSIONS AND CLINICAL RELEVANCE: Application of the MRF is an effective technique for aiding the reduction of acetabular fractures in dogs. It maintains reduction while simultaneously permitting unimpeded access to the dorsal acetabular rim, thus facilitating accurate contouring of a plate. Accurate reduction and rigid fixation of articular fractures is essential to prevent secondary osteoarthritis.  相似文献   

Effects of Exogenous Glutathione on the Quality of Chilled Bull Semen     

MN Munsi  MMU Bhuiyan  S Majumder  MGS Alam 《Reproduction in domestic animals》2007,42(4):358-362

Glutathione (GSH) at concentrations of 0.0 (control), 0.5, 1.0, 2.0 and 3.0 mM was added to chilled bull semen to determine its effects on the keeping quality of semen used for artificial insemination (AI). The semen was preserved with egg yolk citrate extender. All samples were stored at 4-8 degrees C for 5 days. Sperm motility and proportion of abnormal acrosome were assessed daily. Sperm motility was significantly (p < 0.01) higher in the semen treated with 0.5 mM glutathione than in untreated semen on each day. The optimum sperm motility (>or=50%) for AI was retained significantly (p < 0.01) for 3 days in 0.0, 0.5, 1.0 and 2.0 mM glutathione treated semen, whereas in 0.3 mM glutathione-treated semen, sperm motility was 46.8% for 3 days. Acrosomal damage was significantly (p < 0.01) reduced after addition of 0.5 mM GSH in the preserved semen. Bull semen can be preserved in chilled condition for 5 days with 0.5 mM GSH with sperm motility above 40% and 12% acrosome abnormality.  相似文献   

Paratracheal air cyst in a foal     

S. Zetterström  K. Horzmann  J. Yin  R. Moon  S. Boorman  S. Ceriotti  A. Wooldridge  L. Boone 《Equine Veterinary Education》2022,34(5):e187-e192

A 1-day-old American Paint Horse was presented for a large air-filled mass along the ventral aspect of the neck. Bronchoscopy and oesophagoscopy revealed no sign of communication with the trachea or oesophagus. Radiographs and a computed tomography scan of the neck identified a communicating tract between the lumen of the cystic mass and mid-trachea. The foal was systemically healthy at this initial presentation, and delayed removal of the cyst was recommended to allow further maturation of the foal prior to undergoing general anaesthesia. Upon discharge, the cyst continued to grow in size and became more fluid than air-filled requiring repeat centesis and draining. The foal was then re-presented at 3 weeks of age for surgical removal. In surgery, direct communication with the trachea was identified and ligated. Histopathology demonstrated that the cyst lining was composed of squamous epithelium with goblet cells and occasional ciliated cells. The location, morphological features and congenital presentation of the mass were consistent with a paratracheal air cyst (PAC). Surgical resection resulted in excellent functional and cosmetic outcome. Although not previously reported in horses, PAC should be included in the differential diagnosis of an air-filled ventral neck mass in equine neonates. Complete surgical excision may result in a successful outcome.  相似文献   

MALDI‐MS Lipid Profiles of Oocytes Recovered by Ovum Pickup from Bos indicus and 1/2 indicus × taurus with High vs Low Oocyte Yields     

KC Silva‐Santos  CR Ferreira  GMG Santos  MN Eberlin  LS Siloto  CO Rosa  TN Marcantonio  MM Seneda 《Reproduction in domestic animals》2014,49(5):711-718

The aim of the present study was to compare the lipid profile in oocytes of indicus and 1/2 indicus × taurus cows with high and low antral follicle count (AFC)/oocyte yields. After an OPU procedure (D0), antral follicles ≥3 mm were counted by ultrasonography (D4, 19, 34, 49, 64), and cows were assigned to groups with either high AFC (≥30 follicles; indicus, NH group; 1/2 indicus × taurus, AH group) or low AFC (≤15 antral follicles; indicus, NL group; 1/2 indicus × taurus, AL group). The lipid profiles of the oocytes were determined by MALDI‐MS. For GI, GII and GIII oocytes, the indicus samples tend to cluster separately from the 1/2 indicus × taurus samples. The lipid species [PC (P‐38:5) + H]⁺ and/or [PC (P‐36:2) + Na]⁺, [PC (38:2) + H]⁺, [PC (38:5) + Na]⁺ and [TAG (60:8) + NH⁴]⁺ were more abundant in indicus (NH and NL groups) than 1/2 indicus × taurus. The higher lipid content in the indicus oocytes likely reflects differences in the rate of lipid metabolism and may contribute to oocyte competence and embryo development.  相似文献   

Three-dimensional structure of interleukin-2   总被引：17，自引：0，他引：17  

B J Brandhuber  T Boone  W C Kenney  D B McKay 《Science (New York, N.Y.)》1987,238(4834):1707-1709

Interleukin-2 is an effector protein that participates in modulating the immune response; it has become a focal point for the study of lymphokine structure and function. The three-dimensional structure of the interleukin molecule has been solved to 3.0 angstrom resolution. Interleukin-2 has a novel alpha-helical tertiary structure that suggests one portion of the molecule forms a structural scaffold, which underlies the receptor binding facets of the molecule.  相似文献   

Forest biogeochemistry and primary production altered by nitrogen saturation   总被引：1，自引：0，他引：1  

John D. Aber  Alison Magill  Steven G. Mcnulty  Richard D. Boone  Knute J. Nadelhoffer  Marty Downs  Richard Hallett 《Water, air, and soil pollution》1995,85(3):1665-1670

Results from four intensive site-level manipulations and one extensive field survey in northern temperate and boreal forests show a consistent set of responses to chronic N additions. These include 1) initial and often large increase in net N mineralization followed by decreases, 2) increases in net nitrification. 3) increases in N concentration in foliage, and 4) decreased Mg∶N and Ca∶Al ratios, and declining tree growth and vigor in all evergreen stands. These results are synthesized into a set of proposed summary relationships that define the temporal pattern of responses of N-limited systems to N additions.  相似文献   

The interactive effects of UV-B and insecticide exposure on tadpole survival, growth and development     

Christine M. Bridges  Michelle D. Boone 《Biological conservation》2003,113(1):49-54

Because declines within amphibian populations can seldom be attributed to a single cause, it is important to focus on multiple stressors, both natural and anthropogenic. Variables such as UV-B radiation and chemical contamination can interact with one another in ways that might not be predicted from single-factor studies. We exposed southern leopard frog (Rana sphenocephala) tadpoles to the insecticide carbaryl and varying intensities of UV-B radiation in artificial ponds and examined their effects on survival, size at metamorphosis, and the duration of the larval period. Tadpole survival to metamorphosis was positively influenced by UV-B intensity. Tadpoles in ponds exposed to carbaryl contained over three times more algae and yielded larger metamorphs than control ponds. Although previous laboratory studies have indicated carbaryl becomes more toxic in the presence of UV-B, we did not find such an effect, perhaps because of the protection afforded by dissolved organic carbon within the ponds. Our research emphasizes the importance of conducting field studies to more accurately predict what occurs under a natural setting.  相似文献