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Saa LR Perea A Jara DV Arenas AJ Garcia-Bocanegra I Borge C Carbonero A 《Tropical animal health and production》2012,44(7):1423-1427
A cross-sectional study was carried out to determine the seroprevalence and risk factors associated with bovine respiratory syncytial virus (BRSV) infection in non-vaccinated dairy and dual-purpose cattle herds from Ecuador. A total of 2,367 serum samples from 346 herds were collected from June 2008 to February 2009. A questionnaire, which included variables related to cattle, health, management measures, and the environment, was filled out in each herd. Presence of antibodies against BRSV was analyzed using a commercial indirect ELISA test. A logistic regression model was used to determine risk factors associated with BRSV at herd level. The individual seroprevalence against BRSV in non-vaccinated herds in Ecuador was 80.48% [1,905/2,367; 95% confidence interval (CI)?=?78.9-82.1]. The herd prevalence was 91.3% (316/346; 95% CI?=?88.3-94.3), and the intra-herd prevalence ranged between 25% and 100% (mean, 90.47%). The logistic regression model showed that the existence of bordering cattle farms, the dual-purpose farms, and the altitude of the farm (more than 2,338?m above sea level) were risk factors associated with BRSV infection. This is the first study about BRSV prevalence in Ecuador. It shows the wide spread of the BRSV infection in the country. The risk factors found will help to design effective control strategies. 相似文献
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A statistical assessment of the biological relationship between simultaneous canine mammary tumours 
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下载免费PDF全文 Simultaneous canine mammary tumours (CMTs) are frequently reported in the literature, but few studies have addressed their biological relationship in detail or performed statistical assessments. In this study, 269 canine mammary gland tumours from 216 dogs were categorized using an extended histopathological classification, where semiquantitative and binomial scales enumerated morphological parameters of the tumours. The classification facilitated a statistical study of the biological relationship between simultaneous within‐dog tumours. Seventy‐seven percent of the dogs had single tumours and 23% had simultaneous tumours. Sixty‐one percent of the neoplasias were benign, with complex adenoma as the most frequent diagnosis and 39% were malignant, with complex carcinoma as the most common malignancy. Simultaneous tumours within dogs more often had equal diagnoses and neoplastic level (benign or malignant) than would be expected by chance alone, as compared with random pairs of single tumours from different dogs. This statistically supported finding indicated the presence of a biological relationship between simultaneous tumours. 相似文献
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Andreu Colom-Cadena Roser Velarde Jesús Salinas Carmen Borge Ignacio García-Bocanegra Emmanuel Serrano Diana Gassó Ester Bach Encarna Casas-Díaz Jorge R López-Olvera Santiago Lavín Luís León-Vizcaíno Gregorio Mentaberre 《Acta veterinaria Scandinavica》2014,56(1)
Background
In 2010, an Iberian ibex (Capra pyrenaica hispanica) stock reservoir was established for conservation purposes in north-eastern Spain. Eighteen ibexes were captured in the wild and housed in a 17 hectare enclosure. Once in captivity, a caseous lymphadenitis (CLA) outbreak occurred and ibex handlings were carried out at six-month intervals between 2010 and 2013 to perform health examinations and sampling. Treatment with a bacterin-based autovaccine and penicillin G benzatine was added during the third and subsequent handlings, when infection by Corynebacterium pseudotuberculosis was confirmed. Changes in lesion score, serum anti-C. pseudotuberculosis antibodies and haematological parameters were analyzed to assess captivity effects, disease emergence and treatment efficacy. Serum acute phase proteins (APP) Haptoglobin (Hp), Amyloid A (SAA) and Acid Soluble Glycoprotein (ASG) concentrations were also determined to evaluate their usefulness as indicators of clinical status.Once in captivity, 12 out of 14 ibexes (85.7%) seroconverted, preceding the emergence of clinical signs; moreover, TP, WBC, eosinophil and platelet cell counts increased while monocyte and basophil cell counts decreased. After treatment, casualties and fistulas disappeared and both packed cell volume (PCV) and haemoglobin concentration significantly increased. Hp, SAA and ASG values were under the limit of detection or showed no significant differences.Conclusions
A role for captivity in contagion rate is suggested by the increase in antibody levels against C. pseudotuberculosis and the emergence of clinical signs. Although boosted by captivity, this is the first report of an outbreak of caseous lymphadenitis displaying high morbidity and mortality in wild ungulates. Treatment consisting of both vaccination and antibiotic therapy seemed to prevent mortality and alleviate disease severity, but was not reflected in the humoural response. Haematology and APP were not useful indicators in our study, perhaps due to the sampling frequency. Presumably endemic and irrelevant in the wild, this common disease of domestic small ruminants is complicating conservation efforts for the Iberian ibex in north-eastern Spain. 相似文献7.
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Carbonero A. Guzmán L.T. García-Bocanegra I. Borge C. Adaszek L. Arenas A. Saa L. R. 《Tropical animal health and production》2018,50(1):197-203
Tropical Animal Health and Production - An extensive cross-sectional study to determine the seroprevalence of and associated risk factors for Brucella infection was performed in dairy and mixed... 相似文献
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Sidsel Fiskaa Hagen Grethe Iren A. Borge Knut Asbjørn Solhaug Gunnar B. Bengtsson 《Postharvest Biology and Technology》2009,51(1):36-42
In this study, curly kale stored at 1 °C for 3 and 6 weeks was compared with plants remaining in the field regarding several properties related to human health and sensory quality. Cold storage had no effect on the antioxidant capacity (ORAC assay), total phenols (Folin-Ciocalteu assay) or flavonol content, but reduced the content of vitamin C and soluble sugars. The ratio ascorbic:dehydroascorbic acid decreased from about 3 to 0.5 upon storage. The largest changes in the content of bioactive compounds were found in plants that remained in the field for 6 additional weeks, including many frost days. In these plants, the levels of flavonols, total phenols and antioxidant capacity were reduced by 25–35% and the vitamin C content by more than 50%, whereas soluble sugars and dry matter increased by roughly 20% and 30%, respectively. Among all the curly kale samples, the antioxidant capacity was positively correlated with the level of total phenols (r = 0.73, P < 0.001) and total flavonols (r = 0.70, P < 0.001). Values obtained with a non-destructive method based on chlorophyll fluorescence (ChlF) were well correlated with the flavonol content in the leaves, with the highest correlation found for quercetin (r = 0.76, P < 0.001). Using the ChlF method, the kinetics of the flavonol content during the experiment was recorded. The same method also showed gradients of flavonol content within the plant, with the highest values in the upper leaves and in the tip of the leaves. ChlF measurement, therefore, proved to be a quick and cost-effective alternative to chemical analyses of the flavonol content in curly kale. 相似文献
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Carbonero A Saa LR Jara DV García-Bocanegra I Arenas A Borge C Perea A 《Preventive veterinary medicine》2011,100(1):84-88
A cross-sectional study was carried out to determine the seroprevalence and risk factors associated to Bovine Herpesvirus 1 (BHV-1) infection in non-vaccinated dairy and dual purpose cattle herds from Ecuador. A total of 2367 serum samples from 346 herds were collected from June 2008 through February 2009. A questionnaire, which included variables related to cattle, health, management measures and environment was filled out in each herd. A commercial indirect ELISA test was used to determine the seropositivity against BHV-1. Generalized Estimating Equations (GEE) model was used to determine risk factors at individual level, including herd as random effect. The individual seroprevalence to BHV-1 in Ecuador was 43.2% (1023/2367; CI(??%): 41.2-45.2%). The herd prevalence was 82.1%; (284/346; CI(??%): 78.1-86.1%) and the intra-herd prevalence ranged from 12.5 to 100% (mean=64.1%). The GEE model showed that animal age (>4 years) (OR: 1.44; CI(??%): 1.18-1.75), BRSV infection (OR: 1.45; CI(??%): 1.09-1.92), altitude over the sea level (≤ 1800 m) (OR: 2.97; CI(??%): 2.1-4.22) and average slope (> 11%) (OR: 1.45; CI(??%): 1.07-1.95) are risk factors associated with BHV-1 infection, while a good cleaning of the facilities (OR: 0.66; CI(??%): 0.44-0.99) was shown to be a protective factor. 相似文献