Discovering new areas of veterinary science through qualitative research interviews: introductory concepts for veterinarians          下载免费PDF全文

CF May 《Australian veterinary journal》2018,96(8):278-284

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Melatonin reduces apoptotic cells,SOD2 and HSPB1 and improves the in vitro production and quality of bovine blastocysts          下载免费PDF全文

TC Marques  EC da Silva Santos  TO Diesel  LO Leme  CF Martins  MAN Dode  BG Alves  FPH Costa  EB de Oliveira  ML Gambarini 《Reproduction in domestic animals》2018,53(1):226-236

Effects of adding different concentrations of melatonin (10^?7, 10^?9 and 10^?11 M) to maturation (Experiment 1; Control, IVM  + 10^?7, IVM  + 10^?9, IVM  + 10^?11) and culture media (Experiment 2; Control, IVC  + 10^?7, IVC  + 10^?9, IVC  + 10^?11) were evaluated on in vitro bovine embryonic development. The optimal concentration of melatonin (10^?9 M) from Experiments 1–2 was tested in both maturation and/or culture media of Experiment 3 (Control, IVM  + 10^?9, IVC  + 10^?9, IVM /IVC  + 10^?9). In Experiment 1, maturated oocytes from Control and IVM  + 10^?9 treatments showed increased glutathione content, mitochondrial membrane potential and percentage of Grade I blastocysts (40.6% and 43%, respectively). In Experiment 2, an increase in the percentage of Grade I blastocysts was detected in IVC  + 10^?7 (43.5%; 56.7%) and IVC  + 10^?9 (47.4%; 57.4%). Moreover, a lower number and percentage of apoptotic cells in blastocysts were observed in the IVC  + 10^?9 group compared to Control (3.8 ± 0.6; 3.6% versus 6.1 ± 0.6; 5.3%). In Experiment 3, the IVC  + 10^?9 treatment increased percentage of Grade I blastocysts with a lower number of apoptotic cells compared to IVM /IVC  + 10^?9 group (52.6%; 3.0 ± 0.5 versus 46.0%; 5.4 ± 1.0). The IVC  + 10^?9 treatment also had a higher mRNA expression of antioxidant gene (SOD 2) compared to the Control, as well as the heat shock protein (HSPB 1) compared to the IVM  + 10^?9. Reactive oxygen species production was greater in the IVM /IVC  + 10^?9 treatment group. In conclusion, the 10^?9 M concentration of melatonin and the in vitro production phase in which it is used directly affected embryonic development and quality.  相似文献   

Effects of anabolic implants of oestradiol alone or in combination with trenbolone acetate on the ultrastructure of mammary glands in female lambs regarding their interference in prolactin secretion     

Blanco A  Moya L  Flores R  Agüera E  Monterde JG 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2002,49(1):13-17

The side-effects of anabolic steroid implants on mammary gland ultrastructure were evaluated in female lambs treated with oestradiol (n = 10) and with oestradiol plus trenbolone acetate (n = 10). Ten non-implanted lambs were used as controls. Apart from the ultrastructural study of the mammary gland, an assessment of the prolactin pituitary cell population was carried out by immunological methods. Our results showed that oestrogenic implants exert stimulating effects on mammary gland development, both by activating the synthesis process at mammary gland cell levels and by increasing prolactin pituitary production. Nevertheless, there was no evidence of secretory products in the lumen of the gland. Implants containing trenbolone acetate counteracted the mammary stimulus of oestrogens showing ultrastructural images of cell autolysis and necrosis.  相似文献   

Wood of Gmelina arborea in Costa Rica     

Roque  Róger Moya 《New Forests》2004,28(2-3):299-307

New Forests - Gmelina arborea Roxb. (gmelina) was introduced in Costa Rica in the late 1960s. At the present time, gmelina is the most widely planted tree species in Costa Rica. According to...  相似文献   

Tree density and site quality influence on Pinus halepensis Mill. reproductive characteristics after large fires     

Daniel Moya  Josep M. Espelta  Iraima Verkaik  Francisco López-Serrano  Jorge De Las Heras 《Annals of Forest Science》2007,64(6):649-656

In Spain, many Pinus halepensis Mill. forests have been seriously affected by significant forest fires in the past decade, in 1994 alone, more than 100 000 ha were burned in Eastern Spain. In order to study the reproductive characteristics of P. halepensis after forest fires, six locations were selected in four areas affected by serious fires in the summer of 1994, and the following different precipitation zones were studied: dry-subhumid, dry and semi-arid. Ten years after the fires, data relevant to the production of pine cones: serotinous (grey), mature (brown), immature (green) and opened cones, was collected from areas with natural post-fire regeneration. Various cone and seed characteristics, such as pine cone seed number and weight, and germination percentage, were measured. The results showed greater production of cones and strobili in high-density sites. The biggest cone sizes (volume) and seed number per cone were related to site quality (dry-subhumid precipitation zone). Also, viability and germination percentages were higher with better site quality, with significant differences in values for serotinous and non-serotinous cones. Despite these differences, the canopy seed bank was large enough to ensure regeneration in this area for this age stand.  相似文献   

Antimicrobial and Antibiofilm Effect of Inulin-Type Fructans,Used in Synbiotic Combination with Lactobacillus spp. Against Candida albicans     

García-Gamboa  Ricardo  Domínguez-Simi  Miguel Ángel  Gradilla-Hernández  Misael Sebastián  Bravo-Madrigal  Jorge  Moya  Andrés  González-Avila  Marisela 《Plant foods for human nutrition (Dordrecht, Netherlands)》2022,77(2):212-219

Plant Foods for Human Nutrition - There is great interest in the search for new alternatives to antimicrobial drugs, and the use of prebiotics and probiotics is a promising approach to this...  相似文献   

Grouping of <Emphasis Type="Italic">Tectona grandis</Emphasis> (L.f.) clones using wood color and stiffness     

Róger Moya  Juan Diego Marín 《New Forests》2011,42(3):329-345

Commercial Tectona grandis plantations using clones have attracted considerable interest for lumber production in tropical regions where small areas are frequent. However, studies on wood properties are limited. Therefore, the purpose of this study is to group clones with similar wood color characteristics using the CIELab system, and mechanical resistance by dynamic stiffness (Ed) in order to obtain different types of clones for different climate and edaphic conditions. It was found that lightness (L*), yellowness (b*), redness (a*), and Ed are similar to those found for other plantation trees. These wood characteristics are significantly influenced by clone and site. Variance due to clone and site went from 31 to 53% and from 2.95 to 24.22%, respectively. Another source of variance was distance from pith, with the exception of parameter b*, which was not affected. Finally, clones were grouped (using multivariate analysis) according to color and mechanical resistance. This analysis established 4 groups by color and 5 groups by Ed. Clone clustering will allow us to choose or use clones with similar wood properties according to site and environmental conditions increasing area production and wood quality uniformity.  相似文献   

Changes in photosynthesis and antioxidant metabolism of cotton (Gossypium hirsutum L.) plants in response to manganese stress     

Renan Francisco Rimoldi Tavanti  Grace David Queiroz  Anne Caroline Da Rocha Silva  Walmor Moya Peres  Amanda Pereira Paixão  Fernando Shintate Galindo 《Archives of Agronomy and Soil Science》2020,66(6):743-762

ABSTRACT

This study aimed to assess the physiological and biochemical responses of cotton plants to manganese (Mn²⁺) nutrition. Four cotton genotypes (G1 – TMG 47; G2 – FM 975 WS; G3 – TMG 11 WS and G4 – IMA 8405 GLT) were grown in nutrient solution under two Mn²⁺ concentrations (2 and 200 µmol L^?1) for 10 days. No visible symptoms of Mn²⁺ toxicity were observed in the genotypes tested. All genotypes showed a marked increase in leaf chlorophylls, pheophytins, carotenoids, sucrose and total sugars concentration in response to high Mn²⁺ in a nutrient solution. However, the net photosynthetic rate, stomatal conductance, internal carbon dioxide concentration and transpiration decreased in genotypes G1 and G2 growing under 200 µmol L^?1. Antioxidant enzymes such as superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione reductase (GR) activities increased in genotypes G1, G3 and G4. Cotton genotypes showed an increased leaf antioxidant and sugar metabolism as a possible strategy to mitigate oxidative stress. The decrease in the net photosynthetic rate and stomatal conductance; the increased antioxidant enzymes activities (SOD, APX and GR); and the increase in leaf sucrose and total sugar concentration were the main physiological and biochemical responses in cotton plants to Mn²⁺ stress.  相似文献   

Time-resolved spectral studies of blue-green fluorescence of artichoke (Cynara cardunculus L. Var. Scolymus) leaves: identification of chlorogenic acid as one of the major fluorophores and age-mediated changes     

Morales F  Cartelat A  Alvarez-Fernández A  Moya I  Cerovic ZG 《Journal of agricultural and food chemistry》2005,53(25):9668-9678

Synchrotron radiation and the time-correlated single-photon counting technique were used to investigate the spectral and time-resolved characteristics of blue-green fluorescence (BGF) of artichoke leaves. Leaves emitted BGF under ultraviolet (UV) excitation; the abaxial side was much more fluorescent than the adaxial side, and in both cases, the youngest leaves were much more fluorescent than the oldest ones. The BGF of artichoke leaves was dominated by the presence of hydroxycinnamic acids. A decrease in the percentage of BGF attributable to the very short kinetic component (from 42 to 20%), in the shape of the BGF excitation spectra, and chlorogenic acid concentrations indicate that there is a loss of hydroxycinnamic acid with leaf age. Studies on excitation, emission, and synchronized fluorescence spectra of leaves and trichomes and chlorogenic acid contents indicate that chlorogenic acid is one of the main blue-green fluorophores in artichoke leaves. Results of the present study indicate that 20-42% (i.e., the very short kinetic component) of the overall BGF is emitted by chlorogenic acid. Time-resolved BGF measurements could be a means to extract information on chlorogenic acid fluorescence from the overall leaf BGF.  相似文献   

Variation of wood color parameters of Tectona grandis and its relationship with physical environmental factors   总被引：1，自引：0，他引：1  

Róger Moya  Julio Calvo-Alvarado 《Annals of Forest Science》2012,69(8):947-959

? Context

Teak??s wood color is considered an important attribute in the marketing phase and it has been influenced by environmental setting, stand conditions and management, plant genetic source, and age. However, there is a lack of understanding about how the environmental factors might affect the teak??s wood color planted in short-rotation forest plantations.

? Aims

The aim of this study is to understand the relationship, gathered from generated information, between edaphic and climatic variables and their effects in the wood color variation of Tectona grandis from trees in forest plantations.

? Methods

Twenty-two plots were grouped in five cluster sites that shared similar climatic and soil conditions. Data about soil??s physical?Cchemical properties and climatic variables were collected and analyzed. Representative trees were harvested next to each plot in order to obtain a wood sample per tree at a diameter breast height. Wood color was measured using standardized CIELab??s chromaticity system.

? Results

After comparing the wood change color index (?E*) in the five studied clusters, it was found that heartwood produced from drier and fertile sites had more yellowish-brown color. The heartwood b* color index resulted with significant correlations (R?>?0.5, P?<?0.05) among nine climatic and eight edaphic variables.

? Conclusion

It was concluded that climatic variables should be considered as the first-order causal variables to explain wood color variation. Hence, darker b* wood color was associated with dry climates; also, with deeper and fertile sites.  相似文献