The use of ultrasonography in the reproductive evaluation of boars          下载免费PDF全文

RO Pinho  BS Camilo  DMA Lima  FAC Villadiego  JCM Vergara  HH Shiomi  RE Cardoso  PS Lopes  JD Guimarães 《Reproduction in domestic animals》2018,53(2):393-400

The objective was to study the use of ultrasound as a complementary test in the breeding soundness evaluation in male pigs and study the pattern of echogenicity of the testicular parenchyma in boars of different racial groups. Twenty‐six adult boars from four different racial groups were used, 10 from the Piau breed (group 1), four from the commercial and finishing group (group 2), six Pietrain breed (group 3) and six from the Duroc breed (group 4). All animals were evaluated for breeding soundness evaluation and the ultrasound examination of the testicles. The groups of animals that were evaluated showed no difference in the main semen parameters that were evaluated, except for the sperm volume, concentration of the ejaculated sperm and the supravital staining; the lowest figures were for the animals from the Piau breed (group 1). In relation to the testicular biometrics, Duroc animals (group 4) had a greater scrotal width compared to the other groups. But when we assessed the intensity of pixels of the testicles, there was a difference between groups. The groups 2 (finishing animals), 3 (Pietrain) and 4 had no difference between themselves. Group 3 had greater pixel intensity in relation to group 1. Of the 26 animals studied, five showed an abnormality during ultrasound evaluation, like hydrocele, hyperechoic mass in the testicular parenchyma, cyst in the head of the epididymis and the presence of fluid in the head and tail of the epididymis. The various animal groups studied did not differ in the principal reproductive parameters evaluated, showing that despite the great variability of reproductive traits between breeds and within the same breed, the breeding soundness evaluation, the more complete it is, is essential for the selection of breeders and the ultrasonography of the reproductive system becomes an important addition in this examination.  相似文献   

Establishment techniques affect productivity,nutritive value and atmospheric N2 fixation of two sunn hemp cultivars     

David M. Jaramillo  José C. B. Dubeux Jr  Joao M. B. Vendramini  Luana M. D. Queiroz  Erick R. S. Santos  Martin Ruiz-Moreno  Liza Garcia  Daciele Sousa de Abreu  Lucas Ramos de Miranda  Michelle Cristina Fernando de Siqueira 《Grass and Forage Science》2020,75(2):153-158

Nitrogen fertilization is a common practice for sustaining forage production in forage systems in southeastern United States. Warm-season annual legumes may be an alternative forage to warm-season perennial grasses that do not require N fertilization. Sunn hemp (Crotalaria juncea L.) is a fast-growing, warm-season annual legume native to India and Pakistan. The objective of this 2-year study was to assess the herbage accumulation (HA), atmospheric N₂ fixation (ANF) and nutritive value of sunn hemp. Treatments were the factorial arrangement of two sunn hemp cultivars (“Crescent Sun” and “Blue Leaf”), three seeding rates (17, 28 and 39 kg seed/ha) and seed inoculation (inoculated or non-inoculated seeds), distributed in a randomized complete block design with four replicates. Crescent sun had greater HA (3,218 vs. 1764 kg DM/ha) and ANF (41 vs. 25 kg N/ha). Blue leaf had greater crude protein (CP) (188 vs. 176 g/kg) and in vitro digestible organic matter (IVDOM) concentrations (564 vs. 531 g/kg) than crescent sun. Non-inoculated seed had greater CP than inoculated seed, 188 and 177 g/kg, respectively, and inoculation did not affect HA. Intermediate seeding rate (28 kg/ha) decreased HA (2002 kg DM/ha), while HA from high and low seeding rates (17 and 39 kg/ha, respectively) did not differ (2,863 and 2,615 kg DM/ha respectively). Planting non-inoculated crescent sun at 17 kg/ha seeding rate is a feasible management practice to produce sunn hemp in subtropical regions; however, inoculation should always be recommended for proper establishment.  相似文献   

The inflammatory cell infiltrates in porcine cysticercosis: immunohistochemical analysis during various stages of infection     

Londoño DP  Alvarez JI  Trujillo J  Jaramillo MM  Restrepo BI 《Veterinary parasitology》2002,109(3-4):249-259

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First report of Porcine teschovirus (PTV), Porcine sapelovirus (PSV) and Enterovirus G (EV-G) in pig herds of Brazil     

Daiane Güllich Donin  Raquel de Arruda Leme  Alice Fernandes Alfieri  Geraldo Camilo Alberton  Amauri Alcindo Alfieri 《Tropical animal health and production》2014,46(3):523-528

Porcine teschovirus (PTV), Porcine sapelovirus (PSV) and Enterovirus G (EV-G) have been associated with enteric, respiratory, reproductive and neurological disorders. Although Brazil is the world’s fourth largest producer and exporter of pork, no information on the occurrence of PTV, PSV and EV-G infections is available for Brazilian pig herds. This study aimed to investigate the occurrence of Porcine enteric picornavirus infections in pig farms located in three distinct geographical regions of Brazil. Forty randomly selected diarrhoeic and normal consistency faeces of suckling (n?=?22) and nursery (n?=?18) pigs from farms located in 21 distinct cities of the Southern, Southeast, and Midwest regions of Brazil were evaluated by nested-RT-PCR assays. Suckling piglets presented the expected amplicon size for PTV (158 bp) and EV-G (313 bp) in single and mixed infections in 40.9 % (9/22) of the faecal samples. PSV amplicon (212 bp) was not detected in this age group. For nursery pigs, Porcine enteric picornaviruses amplicons were present in 77.8 % (14/18) of the faecal samples. PTV and EV-G were detected in single and mixed infections, while PSV was detected only in two samples in co-infection with PTV and EV-G in this age group. The Brazilian regions evaluated presented at least two of the tested viruses. Sequencing analysis revealed high similarities to the related viruses (95.3 to 99.2 % for PTV, 94.2 to 98.5 % for PSV and 86 to 100 % for EV-G). For the first time PTV, PSV and EV-G have been molecularly detected and characterised in pig faecal samples in Brazil.  相似文献   

Identification of active edge sites for electrochemical H2 evolution from MoS2 nanocatalysts     

Jaramillo TF  Jørgensen KP  Bonde J  Nielsen JH  Horch S  Chorkendorff I 《Science (New York, N.Y.)》2007,317(5834):100-102

The identification of the active sites in heterogeneous catalysis requires a combination of surface sensitive methods and reactivity studies. We determined the active site for hydrogen evolution, a reaction catalyzed by precious metals, on nanoparticulate molybdenum disulfide (MoS2) by atomically resolving the surface of this catalyst before measuring electrochemical activity in solution. By preparing MoS2 nanoparticles of different sizes, we systematically varied the distribution of surface sites on MoS2 nanoparticles on Au(111), which we quantified with scanning tunneling microscopy. Electrocatalytic activity measurements for hydrogen evolution correlate linearly with the number of edge sites on the MoS2 catalyst.  相似文献   

Comment on "The response of vegetation on the Andean flank in western Amazonia to Pleistocene climate change"     

Punyasena SW  Dalling JW  Jaramillo C  Turner BL 《Science (New York, N.Y.)》2011,333(6051):1825; author reply 1825

Cárdenas et al. (Reports, 25 February 2011, p. 1055) used the presence of Podocarpus pollen and wood to infer ≥5°C cooling of Andean forests during Quaternary glacial periods. We show that (i) Podocarpus has a wide elevation range in the Neotropics, and (ii) edaphic factors cannot be discounted as a factor governing its distribution. Paleoecologists should therefore reevaluate Podocarpus as a cool-temperature proxy.  相似文献   

Characterization of lettuce (Lactuca sativa L.) grown with biopesticides and deltamethrin     

Simone Palma Favaro  Yardlei Carlos AlbaAnderson Dias Vieira de Souza  Antonio Camilo Arguelho ViannaAntonia Railda Roel 《Scientia Horticulturae》2011

The purpose of this study was to evaluate the physico-chemical characteristics and the post-harvest loss of mass of curly lettuce (Lactuca sativa L.) cv. Verônica that was grown in a protective environment with applications of biopesticides. The curly lettuce was treated by spraying with neem oil, cassava flour wastewater (manipueira), biofertiliser or the control treatment of deltamethrin. We determined the weight, circumference, dry matter, ash, crude protein, total phenols and minerals profile for the heads of lettuce. After harvesting, the lettuce was packed into opened plastic bags and kept at 5 °C for 5 days to evaluate its weight loss. No differences were observed among the treatments for the physico-chemical parameters. The lettuce that was sprayed with manipueira or biofertiliser had the greatest weight loss during storage. The achievement of similar yields with cheaper products suggests a benefit of using organic bioinsecticides.  相似文献   

Antioxidant activity of ethanolic extracts from several asparagus cultivars   总被引：3，自引：0，他引：3  

Rodríguez R  Jaramillo S  Rodríguez G  Espejo JA  Guillén R  Fernández-Bolaños J  Heredia A  Jiménez A 《Journal of agricultural and food chemistry》2005,53(13):5212-5217

Three different methods (antiradical activity, inhibition of primary oxidation, and ferric reducing power) have been used to evaluate the antioxidant activity of eight different asparagus cultivars and byproducts: white and green asparagus from Alcalá del Río (Guadalquivir Valley, Seville) and American hybrids, native spears, and their byproducts from Huétor-Tájar (Vega de Granada). The correlation between antioxidant activity and total phenol content was studied. Six standards were also tested to validate the modified methods for antioxidant activity determination. Results obtained for antiradical capacity and reducing power were very similar, and a high correlation with phenols was found (R > or = 0.9 for both tests). Sample origin was an important factor, spears from Huétor-Tájar having higher values (ARC between 7 and 10 and P(R) of 0.25-0.33) than those from Alcalá del Río (ARC 0.6-2 and P(R) of 0.05-0.07). Significant differences were found between spears with the same origin, suggesting that genetics are another factor to take into account. Asparagus inhibits lipid primary oxidation, but no correlation between the inhibition percentage and phenols was observed. Asparagus origin was the only factor that led to significant differences: samples from Huétor-Tájar had higher values (POIC between 18 and 32) than those from Alcalá del Río (POIC of 5-9). Byproducts from the canning industry at Huétor-Tájar were also assayed for antioxidant activity; the results obtained suggested that byproducts could be considered as an excellent source of natural antioxidants.  相似文献   

Quantitative electroencephalographic and metabolic studies of midaflur, a new anesthetic agent     

J Jaramillo 《American journal of veterinary research》1979,40(8):1062-1065

Midaflur [4-amino-2,2,5,5-tetrakis(trifluoro methyl)-3-imidazoline] was tested for its ability to induce changes in (i) the EEG of Sprague-Dawley rats with chronically implanted electrodes and (ii) metabolic and kidney functions on Fischer 344 rats. Ataxia and loss of righting reflex were observed in animals given the smaller doses (2 to 5 mg/kg of body weight, intraperitoneally), with little or no effect on the EEG. At higher dose rates, a significant linear dose-response relationship was found for midaflur in depressing brain activity. Changes in body weight and feed and water intake occurred in rats given single doses of midaflur (2 to 20 mg/kg). These effects seemed to be due, in part, to the drug-induced inactivity and to the vehicle in which midaflur has been formulated (equal parts of ethyl alcohol, propylene glycol, and water). Urinary volumes were reduced after injection of midaflur, possibly as a consequence of the changes in water intake. Since midaflur is highly fluorinated, studies were done to detect possible release of inorganic fluoride due to metabolic breakdown. Serum fluoride concentrations in rats given single doses of midaflur (2 to 20 mg/kg) were consistently lower than those of control (untreated) animals, reflecting a reduction in the daily intake of fluoride as a consequence of the diminished feed intake. Significant changes were not observed in the urinary fluoride concentrations in treated rats as compared with these values in control animals given the vehicle alone.  相似文献   

The relationship between the degree of thrombocytopenia and infection with Ehrlichia canis in an endemic area     

Bulla C  Kiomi Takahira R  Pessoa Araújo J  AparecidaTrinca L  Souza Lopes R  Wiedmeyer CE 《Veterinary research》2004,35(1):141-146

Ehrlichia canis is the causative agent of canine monocytic ehrlichiosis. In order to evaluate platelet counts as a screening test for E. canis in an endemic area, 217 whole blood samples from dogs were divided into three groups: 71 non-thrombocytopenic samples (group A, platelet counts greater than 200 000/mL) and 146 thrombocytopenic samples (less than 200 000/mL). The thrombocytopenic group was further divided into 62 with platelet counts between 100 000-200 000/mL (Group B) and 84 samples with less than 100 000 platelets/mL (Group C). All samples were examined for the presence of a segment of the Ehrlichia canis 16S rRNA gene using a nested polymerase chain reaction. Sixty-seven of the 217 samples (30.9%) were positive for the presence of the E. canis 16S rRNA gene; 53 (63.1%) of the group C samples and 13 (21%) of group B. Only one (1.4%) of the non-thrombocytopenic samples (Group A) was positive. These data support the concept that platelet counts may be a good screening test for canine monocytic ehrlichiosis, and that the magnitude of thrombocytopenia may increase the reliability of diagnosis.  相似文献