Subconjunctival cyst associated with Thelazia gulosa in a calf.     

P E Miller  B G Campbell 《Journal of the American Veterinary Medical Association》1992,201(7):1058-1060

A large subconjunctival cyst of 4 weeks' duration was surgically excised from the left eye of a 7-month-old Simmental calf. Two white, partially mineralized, approximately 1-cm-long, fertile female nematodes subsequently identified as Thelazia gulosa were found embedded in the cyst wall. Thelazia spp are generally regarded as nonpathogenic or mildly pathogenic in cattle in North America, despite reported infection rates ranging from 12.2 to 34.2%. Additionally, parasitic subconjunctival nodules associated with Thelazia spp rarely have been reported in the past, and cyst formation has not been described. It was postulated that in this calf, immature or adult worms may have penetrated normal tissue barriers, or entered via an earlier conjunctival wound, and created an inflammatory response with subsequent cyst formation.  相似文献   

安普霉素对仔猪内分泌的调控作用及血液生化指示的影响   总被引：4，自引：0，他引：4  

姚浪群  萨仁娜  佟建明  霍启光 《动物营养学报》2003,15(2):58-64

采用单因子试验设计 ,28日龄大长北三元杂交断奶仔猪72头随机分为3组 ,研究饲料中添加不同剂量的安普霉素 (0、20、90mg/kg)对仔猪内分泌的调控作用及血液生化指标的影响。试验期为4周。结果表明 :仔猪日粮中添加90mg/kg的安普霉素可促进机体与生长有关的内分泌活动 ,提高内源激素 (生长激素、胰岛素、甲状腺激素T3)水平 (P<0.05),从而促进肌肉蛋白沉积 ;并具有显著降低血液中氨、尿素氮含量和提高血糖水平的作用 (P<0.05) ,表明安普霉素对仔猪具有增加氮沉积 ,促进蛋白质合成、抑制蛋白质分解的作用  相似文献   

Studies on efficacy and stability of a vaccine bait containing ERA strain of rabies virus propagated in a BHK-21 cell line.          下载免费PDF全文

K F Lawson  H Chiu  M Matson  P Bachmann    J B Campbell 《Canadian journal of veterinary research》1992,56(2):135-141

In a dose response study in foxes, the median protective dose of ERA BHK21 vaccine in a blister pack bait was 10(6.0) tissue culture infective doses (TCID)/mL, while artificially aged baits with titers of 10(6.3) TCID/mL induced seroconversion in 78% of foxes. There was no significant difference in the development of antibodies in foxes receiving 1, 2 or 3 mL volumes of vaccine in the bait. When baits were exposed to the elements and fed to foxes over a 21 day period, 85% of the animals seroconverted. Age, sex and the way in which the vaccine container was contacted did not appear to be factors in the responses of these animals. Juvenile foxes, approximately six months of age, were marked more readily with the tetracycline bait marker than older animals. Approximately 25% of foxes did not appear to respond well to vaccination and the titer of the vaccine was a critical factor in producing seroconversion in these animals.  相似文献   

金霉素与赖氨酸和蛋氨酸交互作用研究     

佟建明  萨仁娜 《畜牧兽医学报》2002,33(2):110-113

采用3×2×2因子饲养试验和2×2×2因子代谢试验,研究金霉素与赖氨酸和蛋氨酸的交互作用对肉仔鸡生产性能的影响,及金霉素对两种氨基酸代谢的影响。金霉素在饲料中添加150 mg/kg时,对0-3周肉仔鸡具有显著的促生长作用（P<0.01）;并显著提高肉仔鸡的饲料采食量和饲料转化率（P<0.01）,促进氮沉积（P<0.01）。对4-6周肉仔鸡的生产性能无显著影响（P>0.05）;金霉素、赖氨酸和蛋氨酸存在显著的互作关系（P<0.05）。金霉素的促生长效果受饲料中赖氨酸和蛋氨酸含量的影响,当日粮中赖氨酸水平为1.3％,蛋氨酸水平为0.6％时,肉仔鸡的生长性能最高。金霉素对赖氨酸的利用率没有显著影响（P>0.05）,对蛋氨酸和胱氨酸的表观利用率具有显著抑制作用（P<0.05）。研究结果表明持续低剂量金霉素与蛋氨酸和赖氨酸具有交互作用,这种互作关系影响彼此对肉仔鸡生产性能的作用效果,金霉素具有提高肉仔鸡赖氨酸需要量的作用。  相似文献   

Cholelithiasis in a cat     

G L Heidner  K L Campbell 《Journal of the American Veterinary Medical Association》1985,186(2):176-177

A 4-year-old cat was examined because of anorexia and lethargy. The cat became icteric within 3 days of admission. Values for aspartate transaminase, alanine transaminase, total bilirubin, alkaline phosphatase, and cholesterol were higher than normal. Radiography revealed hepatomegaly, with loss of detail in the cranioventral portion of the abdomen. Further diagnostic procedures were not permitted, and the cat was euthanatized. At necropsy, cholecystitis, cholangitis, and numerous choleliths were found. Cholelithiasis is a rare cause of obstructive jaundice in the cat.  相似文献   

The nutritional value of low lathyrogenic Lathyrus (Lathyrus sativus) for growing chicks     

R. G. Rotter  R. R. Marquardt  C. G. Campbell 《British poultry science》1991,32(5):1055-1067

1. The feeding value of new low β‐N‐oxalyl‐amino‐L‐alanine (BOAA) lines of Lathyrus sativus (lathyrus) and the benefits of dehulling the seed or of pre‐adapting chicks to lathyrus‐based diets were examined in several experiments.

2. Chicks fed on diets containing 400 g/kg of the low (1.3 g BOAA/kg seed) and medium (2.2 g BOAA/kg seed) BOAA lines did not differ (P>0.05) in weight gain (WG) or in apparent fat and protein digestibilities compared to birds fed on a wheat‐based diet.

3. Consumption of 600 g low BOAA lathyrus/kg diet caused a slight, but significant (P< 0.05), decrease in WG. Food efficiency decreased as the amount of dietary lathyrus was increased, suggesting that lathyrus may contain an antinutritive factor(s) other than BOAA.

4. Removal of the hull (70 g hull/kg seed) from the seed did not affect chick performance (P> 0.05).

5. Pre‐adapting chicks for 7 d to diets containing up to 600 g medium line lathyrus/kg diet did not reduce the detrimental effects of the lathyrus.

6. Although dehulling and pre‐adaptation of chicks to lathyrus were not beneficial, the low and medium lines of lathyrus tested show potential for use in chick diets up to at least 400 g lathyrus/kg diet.  相似文献   

Male pseudohermaphroditism of the testicular feminization type in a heifer   总被引：1，自引：1，他引：0       下载免费PDF全文

Peter AT  Scheidt AB  Campbell JW  Hahn KA 《The Canadian veterinary journal. La revue veterinaire canadienne》1993,34(5):304-305

  相似文献   

A survey of the selenium status of beef cows in Alberta.   总被引：1，自引：1，他引：0  

J R Campbell  G K Jim  C W Booker    P T Guichon 《The Canadian veterinary journal. La revue veterinaire canadienne》1995,36(11):698-702

An epidemiological survey was conducted in Alberta to measure the selenium status in blood of beef cows during the fall and to determine the risk of selenium deficiency among specific geographic regions of Alberta. Three census divisions of Alberta based on the Statistics Canada Census of Agriculture were chosen as the study areas for the project. Soils and plants in area A (Edmonton area) and area B (Calgary area) were known to be deficient in selenium, while soils and plants in area C (southeast corner of Alberta) were known to have adequate levels of selenium. Blood samples were collected from 335 cows on 29 farms from the 3 study areas. These samples were collected from cows that had recently been removed from pasture in October and November 1992. Answers to a short questionnaire pertaining to various herd characteristics and management practices were also obtained for each herd. The average value of selenium for all cows sampled was 2.20 mumol/L. The average value of selenium of cows in areas A and B was 1.93 mumol/L. The average value of selenium of cows in area C was significantly (P < 0.05) higher at 2.70 mumol/L. Nine percent of the cows in the study were considered marginal or deficient in selenium (< 1.27 mumol/L selenium). Herds located in area C, herds that were provided with supplemental feed on pasture, and herds that were pregnancy checked had higher average herd selenium values than did other herds. Cow-calf producers located in areas with selenium-deficient soils should pay particular attention to selenium supplementation for their cows. Some of the negative "geographic" effects on selenium values can be overcome by more progressive management practices.  相似文献   

Effects of Confluent, Roscovitine Treatment and Serum Starvation on the Cell-cycle Synchronization of Bovine Foetal Fibroblasts     

SR Cho  SA Ock  JG Yoo  B Mohana kumar  SY Choe  GJ Rho 《Reproduction in domestic animals》2005,40(2):171-176

The present study was designed to examine the effects of cell-cycle synchronization protocols, such as confluent, roscovitine treatment and serum starvation, in bovine foetal fibroblasts on synchronization accuracy at G0/G1, viability, apoptosis, necrosis and ploidy for use as a nuclei donor. The cells in 5-10 passages were randomly allocated into three treated groups. Cells were cultured either in Dulbecco's modified Eagle's medium (DMEM) + 10% foetal bovine serum (FBS) until 90% confluent (group 1, confluent), in DMEM + 10% FBS + 30 microM roscovitine for 12 h (group 2, roscovitine), or in DMEM + 0.5% FBS for 5 days (group 3, serum starvation). Most of the cells (>80%) in all groups were arrested at the G0/G1 stage. Although the rates did not differ, cells in group 1 showed an increased cell population arrested at the G0/G1 phase. Significantly (p < 0.05) higher rates of apoptosis occurred in group 3 than in group 1 and 2 (10% vs 6% and 6%, respectively). No differences in chromosomal abnormality were observed among groups. However, by increasing the number of cell culture passages up to 15, significantly (p < 0.05) higher chromosomal abnormality was observed than in 5 and 10 passages (39% vs 28% and 23%, respectively) in group 1. The results clearly indicated that bovine foetal fibroblasts could be effectively synchronized at G0/G1 stages by all the three different treatments, confluent, roscovitine and serum starvation. However, cells in confluent showed reduced apoptosis and necrosis when they underwent 5-10 passages, exhibiting increased percentage of cells with stable chromosome diversity. Hence, cells in confluent merit further studies before they could be used as nuclear donors.  相似文献   

Leaf, floret and seed infection of wheat by Pyrenophora semeniperda     

M. A. Campbell  R. W. Medd †‡ 《Plant pathology》2003,52(4):437-447

Infection processes of Pyrenophora semeniperda on seedling and adult wheat leaves and wheat ears were investigated. Almost 100% germination of conidia occurred on seedling leaves, compared with 20–30% on adult leaves. Appressoria formed over the anticlinal epidermal cell walls and haloes always accompanied infection. Sometimes papillae formed within the leaves as a resistance mechanism. Infection hyphae ramified through the intercellular spaces of the mesophyll resulting in cellular disruption. The infection processes on floral tissues were similar to those observed on leaves; however, no infection occurred on anther, stigmatic or stylar tissues. Infection of ovarian tissue occurred both with and without appressoria formation. Hyphae grew mainly in the epidermal layers and appeared unable to breach the integumental layer as no growth was observed in endosperm or embryo tissues. The optimum dew period temperature for conidial germination was 23·6°C, compared with 19·9°C for lesion development, 20·4°C for the production of infection structures on seedling leaves and 23·7°C for floret infection. Leaf disease development occurred in a logistic manner in response to dew period, with maximum infection observed after 21 h compared with > 48 h in seeds. An initial dark phase during the dew period was necessary for infection and temperature after the dew period had an effect, with significantly more numerous and larger lesions being formed at 15°C compared with 30°C. Seedling leaves were found to be more susceptible than older leaves, under both field and controlled environment conditions. Infection of wheat seeds following inoculation of ears, or after harvest burial of inoculated disease-free seeds, was demonstrated. In the latter, 3-week-old seedlings were slightly stunted, whereas older plants were unaffected. The apparent unimportance of this plant pathogen as a cause of leaf disease in relation to its poor adaptation to dew periods and dew period temperature is discussed, along with the importance of its seed borne characteristics.  相似文献