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Kentarou IKUTA Kazumi SASAKURA Kazuhiro NISHIMORI Careen HANKANGA Keiji OKADA Jun YASUDA 《Animal Science Journal》2005,76(1):29-36
The present study examined the effects of feeding order of grain and protein supplements on ruminal nitrogen (N) loss through the diurnal variation of ruminal ammonia N and urea N in the blood and milk of dairy cows. Three ruminally fistulated cows were used in a 3 × 3 Latin square arrangement of treatments; that is, grain supplement fed at the first feed and then the protein supplement at the second (GS‐1st), protein supplement fed first and then the grain supplement (PS‐1st), and the grain and protein supplements mixed and divided into equal portions fed at each supplement feeding time (GP‐mix). No differences in the lactation parameters were observed among the treatments. In GS‐1st treatment, the increase in the concentration of ruminal ammonia N was inhibited after feeding, and the concentration was lower (P < 0.05) than with the other treatments 1–2 h after basal feeding. The concentrations of urea N in the blood and milk with the GS‐1st treatment were the lowest of all treatments throughout the sampling times, and were lower (P < 0.05) than with the GP‐mix treatment except for blood levels 1 h after basal feeding. The results suggest that grain feeding before protein feeding can reduce ruminal N loss. 相似文献
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Yamada Y Sato R Kobayashi S Hankanga C Inanami O Kuwabara M Momota Y Tomizawa N Yasuda J 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(5):443-448
Lactoferrin has several biological activities, including antitumor activities in some human and animal tumor cells. Clinical trials have been carried out in human medicine based on these effects. However, the antitumor effects of lactoferrin in veterinary medicine remain unknown. In this in vitro study, we demonstrated that co-incubation of canine mammary gland tumor cells (CIPp and CHMp) and bovine lactoferrin induced growth arrest of tumor cells. This growth arrest was associated with induction of G1 arrest. Furthermore, this effect was stronger in tumor cells than in normal cells. These findings demonstrate that bovine lactoferrin has anti-tumor activity in canine mammary tumors and has the potential for use in tumor-bearing dogs. 相似文献
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Kobayashi S Sato R Aoki T Omoe K Inanami O Hankanga C Yamada Y Tomizawa N Yasuda J Sasaki J 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(5):429-435
Feline immunodeficiency virus (FIV) infection is characterized by chronic overactivation of immune and inflammatory system, resulting in anergic state and dysfunction of immune cells. Lactoferrin (LF), a glycoprotein present in exocrine secretions and neutrophils, plays an important role in host defense system. Our previous study showed that oral administration of bovine LF (bLF) suppressed oral inflammation, improved the clinical symptoms and decreased serum gamma-globulin as a marker of inflammation in FIV-infected cats with intractable stomatitis. The anti-inflammatory effect was partly involved in regulation of neutrophil function by bLF. In this study, to clarify the relationship between anti-inflammatory effects of bLF and peripheral blood mononuclear cells (PBMC), we examined the effect of bLF on proliferation, cell cycle progression and cytokine expression in mitogen-activated PBMC. MTT [3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl tetrazolium bromide] assay showed that bLF inhibited the concanavalin A (ConA)-induced cell proliferation in FIV-infected cats with the asymptomatic carrier and AIDS-related complex (ARC) phase. Bovine LF restored ConA-induced cell cycle progression and resulted in suppression of the induced apoptosis in feline PBMC. Real-time RT-PCR showed that bLF suppressed ConA-induced expression of interferon-gamma and interleukin-2 in cells of the ARC group regardless of the time of its addition to the medium. These results suggest the hypothesis that therapy with bLF may have the potential to improve and protect functions of overactivated lymphocytes by modulating the cell proliferation, cell cycle and cytokines expression in cats in terminal stage of FIV infection. 相似文献
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This study of 1196 dogs over a period of 18 months determined the seasonal infection patterns of canine babesiosis in Lusaka, the capital city of Zambia. The work also describes a retrospective study of the prevalence of canine babesiosis in laboratory clinical blood samples submitted to the University of Zambia, School of Veterinary Medicine for routine haematological examination from the year 1994 to 2009. A cross-sectional study was also performed to determine the levels of Babesia in a low-income society (during the dry season and the wet season of the year), where 361 samples were collected from dogs presented for mass rabies vaccination campaigns. Morphology of the Babesia indicated that all were of the large-sized Babesia canis infection. Babesia-positive dogs had significantly higher rectal temperatures than negative ones, and dogs younger than 1 year were more likely to be Babesia positive followed by those between 2 and 5 years old. Seasonal trends indicate two peaks, one in the rainy season (November-March) and another in the cold dry season (June/July). Monthly prevalence rates of Babesia ranged from 0% to 2.4% in natural populations and from 0% to 28.6% in laboratory specimens. This study shows that Zambia has lower Babesia prevalence than reported in other African countries. 相似文献
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Hankanga C Kobayashi S Yamada Y Momota Y Tomizawa N Sato R Yasuda J 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(9):881-885
Adenosine deaminase (ADA), an enzyme involved in purine metabolism, has been shown to be of clinical importance in several diseases in humans. To investigate whether ADA is of any clinical significance in cats, plasma adenosine deaminase (P-ADA) and T cell adenosine deaminase (T-ADA) activities were measured in feline immunodeficiency virus (FIV) negative and positive cats. The AIDS-related complex (ARC) group showed a significant elevation in P-ADA activity compared to the asymptomatic carrier (AC), and FIV-negative groups (P<0.005). T-ADA activity was significantly elevated in FIV-positive cats compared to the FIV-negative group (P<0.05) and this elevation was attributed to the increase in the ARC group (P<0.01). A correlation was found between P-ADA and T-ADA activities in the FIV-negative group. T-ADA activity and CD4(+)cell number showed a strong negative correlation in FIV-positive cats (P<0.0005). CD4(+) cell numbers were significantly reduced in the ARC group compared to the healthy controls (P<0.005). Our results showed that T-ADA is increased in FIV-positive cats during the ARC stage. These results also suggest that ADA may be an indicator of T cell activation in the ARC stage of FIV infection. 相似文献
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