Influence of the Th2 immune response established by Nippostrongylus brasiliensis infection on the protection offered by different vaccines against Chlamydophila abortus infection     

Caro MR  Buendía AJ  Ortega N  Gallego MC  Martínez CM  Cuello F  Ruiz-Ybañez MR  Erb KJ  Salinas J 《Veterinary research communications》2005,29(Z1):51-59

Chlamydophila abortus is the aetiological agent of enzootic abortion in small ruminants in which it infects the placenta to cause abortion during the last trimester of gestation. In a mouse model, a Th1 immune response involving IFN-gamma production and CD8+ T cells is necessary for the infection to be resolved. The authors previously demonstrated that infection with Nippostrongylus brasiliensis, a rodent gastrointestinal nematode extensively used in experimental models to induce Th2 responses, alters the specific immune response against C. abortus infection, increasing bacterial multiplication in liver and reducing specific IFN-gamma production. The aim of the present work was to clarify whether a Th2 immune response has any influence on the success of vaccination using both inactivated and attenuated vaccines. The results showed that the Th2 response established prior to vaccination did not influence the induction of protection offered by the vaccines. However, the effectiveness of this protective response can be altered, depending on the adjuvant employed in the inactivated vaccines, when the Th2 response is established after vaccination, just before challenge with C. abortus.  相似文献   

Field evaluation of a new commercially available ELISA based on a recombinant antigen for diagnosing Chlamydophila abortus (Chlamydia psittaci serotype 1) infection     

Buendía AJ  Cuello F  Del Rio L  Gallego MC  Caro MR  Salinas J 《Veterinary microbiology》2001,78(3):229-239

A new commercially available ELISA (ELISAr-Chlamydia) for detecting antibodies against Chlamydophila abortus has been evaluated using sheep field serum samples. The ELISA is based on a recombinant antigen which expresses part of a protein from the 80-90kDa family that is specific to C. abortus. Sera (105) from six flocks with confirmed ovine chlamydial abortion (OEA) outbreaks were used in this study, as well as sera (258) from 18 flocks which had suffered no OEA in the last lambing. The ELISAr-Chlamydia was compared with the complement fixation test (CFT) and with an ELISA using purified C. abortus elementary bodies (ELISA-EB), employing as reference technique a comparative microimmunofluorescence test that differentiates C. abortus infection from Chlamydophila pecorum infection. The results showed that the sensitivity of ELISAr-Chlamydia was 90.9% with a specificity of 85.9%, the sensitivity of CFT was 71.0% with a specificity of 83.6%, while the sensitivity of ELISA-EB was 95.2% and the specificity was 54.2%. Furthermore, ELISAr-Chlamydia was the test with fewer false positives resulting from positive reactivity to C. pecorum, although 15% of the sera positive for C. pecorum but negative for C. abortus antibodies reacted positively. This study demonstrated with field material that ELISAr-Chlamydia provides the most balanced results between sensitivity and specificity, especially in flocks with no clinical OEA but reactivity to C. abortus.  相似文献   

Animal breeding systems and big game hunting: Models and application     

T.M. Caro  C.R. Young  A.E. Cauldwell 《Biological conservation》2009,142(4):909-929

We apply an age- and stage-structured model incorporating varying harem sizes, paternal care and infanticide to examine the effect of hunting on sustainability of populations. Compared to standard carnivore and herbivore models, these models produce different outcomes for sustainable offtake when either adults, or adult males are harvested. Larger harem size increases sustainable offtake whereas paternal care and infanticide lowers it. Where males are monogamous, populations are vulnerable to male offtake, regardless of paternal care. Surprisingly, an incidental take of 10% of other age-sex-classes has very little effect on these findings. Indiscriminate (subsistence) hunting of all age-sex classes has a dramatic effect on certain populations. Applying these behavior-sensitive models to tourist hunting in the Selous Game Reserve, Tanzania, we find that across the Reserve hunting quotas were generally set at sustainable rates except for leopard (Panthera pardus). In certain hunting blocks within the Reserve, however, quotas for eland (Taurotragus oryx), hartebeest (Alcelaphus buselaphus), lion (Panthera leo), reedbuck (Redunca arundinum), sable antelope (Hippotragus niger), warthog (Phacochoerus aethiopicus) and waterbuck (Kobus ellipsiprymnus) are set at unsustainably high rates. Moreover, particular blocks are consistently awarded high quotas. Behaviorally sensitive models refine predictions for population viability, specify data required to make predictions robust, and demonstrate the necessity of incorporating behavioral ecological knowledge in conservation and management.  相似文献   

Comparison of postharvest changes in mango (cv Cogshall) using a Ripening class index (Rci) for different carbon supplies and harvest dates     

Jacques Joas  Yanis Caro  Mathieu Lechaudel 《Postharvest Biology and Technology》2009,54(1):25-31

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Isolation and characterization of Avenin-like protein type-B from durum wheat     

Salvatore De Caro  Pasquale Ferranti  Francesco Addeo  Gianfranco Mamone 《Journal of Cereal Science》2010

Proteomic techniques were applied for the investigation of “avenin-like protein type-B” in tetraploid wheat. Protein was extracted from endosperm, according to a classical chromatographic procedure and its identity confirmed by liquid chromatography-tandem mass spectrometry analysis. The characterization of avenin-like protein type-B was in good agreement with the gene-derived sequence, with the exception of glutamine as N-terminus. The presence in the glutenin extract and the high number of cysteine residues suggested that avenin-like protein type-B is integrated into gluten polymers via inter-chain disulphide bonds. This raises the question of whether the protein could play a role in determining the functional properties of gluten.  相似文献   

Is earthworms' dispersal facilitated by the ecosystem engineering activities of conspecifics?     

Ga?l Caro  Anick Abourachid  Thibaud Deca?ns  Lorenza Buono  Jér?me Mathieu 《Biology and Fertility of Soils》2012,48(8):961-965

In this work, we documented the influence of earthworm's galleries on their speed of movements during dispersal events in the soil. We quantified, by using X-rays, the dispersal behaviour of earthworms in the soil. The observations were conducted in mesocosms in controlled conditions for 12?h. Our experiments revealed that during a dispersal sequence of a batch of individuals of the species Aporrectodea terrestris (Savigny 1826): (a) individuals used preferentially existing conspecifics' galleries, (b) individual velocity increased after each dispersal event and (c) the lag time before each dispersal event did not seem to be influenced by previous dispersers. Therefore, dispersal seems to be facilitated by conspecifics' activity, which strongly supports the hypothesis of a feedback between ecosystem engineers' activity and their dispersal speed.  相似文献   

Sero-epidemiological survey of porcine respiratory coronavirus (PRCV) infection in breeding herds in southeastern Spain.     

M J Cubero  L Leon  A Contreras  I Lanza  E Zamora  M R Caro 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1992,39(4):290-298

In 1987 we conducted a sero-epidemiological survey in the Murcia Region (South-East Spain) to discover the prevalence and spread of PRCV-infection among breeding pigs and farms and determine the association between herd size and geographical zone with PRCV-infection. The Murcia Region was divided into four geographical zones and the farms classified by size in four categories. The random sample was statistically representative of both the breeding stock and farms in each geographical zone. We analysed 6,000 breeding pigs from 480 farms. The immunological techniques employed were indirect ELISA and blocking ELISA. The prevalence (P +/- IC) of PRCV-seropositive breeding pigs and infected breeding farms was 14.53 +/- 0.89% and 21.87 +/- 7.83% respectively. On 55% of the infected farms, the prevalence of seropositive breeding pigs was 60-100%. PRCV-infection appears spread throughout the four geographical zones of the Murcia Region. However, a significant association (p less than 0.01) was observed between geographical zone and the prevalence of PRCV-infection. A herd size of greater than 50 breeding pigs had a greater risk (p less than 0.01) of PRCV-infection.  相似文献   

Influence of the Th2 immune response established by Nippostrongylus brasiliensis infection on the protection offered by different vaccines against Chlamydophila abortus infection     

Caro  M. R.  Buend&#;a  A. J.  Ortega  N.  Gallego  M. C.  Mart&#;nez  C. M.  Cuello  F.  Ruiz-Yba&#;ez  M. R.  Erb  K. J.  Salinas  J. 《Veterinary research communications》2005,29(1):51-59

Chlamydophila abortus is the aetiological agent of enzootic abortion in small ruminants in which it infects the placenta to cause abortion during the last trimester of gestation. In a mouse model, a Th1 immune response involving IFN-γ production and CD8⁺ T cells is necessary for the infection to be resolved. The authors previously demonstrated that infection with Nippostrongylus brasiliensis, a rodent gastrointestinal nematode extensively used in experimental models to induce Th2 responses, alters the specific immune response against C. abortus infection, increasing bacterial multiplication in liver and reducing specific IFN-γ production. The aim of the present work was to clarify whether a Th2 immune response has any influence on the success of vaccination using both inactivated and attenuated vaccines. The results showed that the Th2 response established prior to vaccination did not influence the induction of protection offered by the vaccines. However, the effectiveness of this protective response can be altered, depending on the adjuvant employed in the inactivated vaccines, when the Th2 response is established after vaccination, just before challenge with C. abortus.  相似文献   

Automatic determination of calcium and magnesium by coulometric titration     

A. Cladera  A. Caro  J. M. Estela  V. Cerda 《Water, air, and soil pollution》1991,59(3-4):321-331

An automatic system based on the use of a PC-compatible computer was developed for the implementation of coulometric titrations by release of EDTA from its Hg complex on electrochemical reduction of the Hg²⁺ ion. A variety of indicators electrodes were assayed to obtain potentiometric titration curves. The method thus developed allows the straightforward, convenient determination of Ca and Mg and was applied to the analysis of various real samples with good results.  相似文献   

Dissipation of soil-incorporated carbofuran in the field   总被引：1，自引：0，他引：1  

J H Caro  H P Freeman  D E Glotfelty  B C Turner  W M Edwards 《Journal of agricultural and food chemistry》1973,21(6):1010-1015

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