Retracted: Effect of Post‐Thaw Storage Time on Motility and Fertility of Cryopreserved Beluga Sturgeon (Huso huso) Sperm          下载免费PDF全文

MS Aramli  RM Nazari  MR Gharibi 《Reproduction in domestic animals》2015,50(2):349-352

The aim of this study was to test the influence of post ‐ thaw storage time on the duration of sperm motility, percentage of motile sperm, and fertilization and hatching rates of fresh sperm and sperm stored for 0, 30 and 60 min at 4°C post‐thawing. After being frozen in liquid nitrogen and then thawed, the percentage of motile sperm and duration of motility were not affected by 30 min of storage at 4°C, whereas a significant decline in these parameters was observed after 60 min of storage. Similarly, fertilization and hatching rates were significantly affected within 60 min of storage at 4°C, and the fertility of frozen‐thawed sperm was significantly lower than that of fresh sperm. We conclude that cryopreserved sperm of beluga sturgeon could be stored for 30 min without the loss of sperm quality. This described procedure for beluga sturgeon cryopreservation is reliable and efficient and therefore can be recommended for hatchery practice after scaling up this technique.  相似文献   

The Influence of Morphology,Follicle Size and Bcl‐2 and Bax Transcripts on the Developmental Competence of Bovine Oocytes     

THC De Bem  PR Adona  FF Bressan  LG Mesquita  MR Chiaratti  FV Meirelles  CLV Leal 《Reproduction in domestic animals》2014,49(4):576-583

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Rhodococcus equi-associated osteomyelitis in foals     

EC FIRTH  MR ALLEY†  H HODGE 《Australian veterinary journal》1993,70(8):304-307

Two cases of Rhodococcus equl infection in foals are described, in which osteomyelitis was a feature. Because rhodococcal infection is usually low grade and chronic, and because the signs of early metaphysitis can be subtle, any articular or periarticular swelling in a foal from a farm with a history of rhodococcosis should be strongly suspected to be associated with R equl until proven otherwise.  相似文献   

Reply     

MR ALDERTON 《Australian veterinary journal》1993,70(11):429-430

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Hydrops foetalis in dwarf calves associated with twinning     

PAW HARPER  MR LATTER  JF WILKINS 《Australian veterinary journal》1995,72(6):236-238

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Influence of the Th2 immune response established by Nippostrongylus brasiliensis infection on the protection offered by different vaccines against Chlamydophila abortus infection     

Caro MR  Buendía AJ  Ortega N  Gallego MC  Martínez CM  Cuello F  Ruiz-Ybañez MR  Erb KJ  Salinas J 《Veterinary research communications》2005,29(Z1):51-59

Chlamydophila abortus is the aetiological agent of enzootic abortion in small ruminants in which it infects the placenta to cause abortion during the last trimester of gestation. In a mouse model, a Th1 immune response involving IFN-gamma production and CD8+ T cells is necessary for the infection to be resolved. The authors previously demonstrated that infection with Nippostrongylus brasiliensis, a rodent gastrointestinal nematode extensively used in experimental models to induce Th2 responses, alters the specific immune response against C. abortus infection, increasing bacterial multiplication in liver and reducing specific IFN-gamma production. The aim of the present work was to clarify whether a Th2 immune response has any influence on the success of vaccination using both inactivated and attenuated vaccines. The results showed that the Th2 response established prior to vaccination did not influence the induction of protection offered by the vaccines. However, the effectiveness of this protective response can be altered, depending on the adjuvant employed in the inactivated vaccines, when the Th2 response is established after vaccination, just before challenge with C. abortus.  相似文献   

Field evaluation of a new commercially available ELISA based on a recombinant antigen for diagnosing Chlamydophila abortus (Chlamydia psittaci serotype 1) infection     

Buendía AJ  Cuello F  Del Rio L  Gallego MC  Caro MR  Salinas J 《Veterinary microbiology》2001,78(3):229-239

A new commercially available ELISA (ELISAr-Chlamydia) for detecting antibodies against Chlamydophila abortus has been evaluated using sheep field serum samples. The ELISA is based on a recombinant antigen which expresses part of a protein from the 80-90kDa family that is specific to C. abortus. Sera (105) from six flocks with confirmed ovine chlamydial abortion (OEA) outbreaks were used in this study, as well as sera (258) from 18 flocks which had suffered no OEA in the last lambing. The ELISAr-Chlamydia was compared with the complement fixation test (CFT) and with an ELISA using purified C. abortus elementary bodies (ELISA-EB), employing as reference technique a comparative microimmunofluorescence test that differentiates C. abortus infection from Chlamydophila pecorum infection. The results showed that the sensitivity of ELISAr-Chlamydia was 90.9% with a specificity of 85.9%, the sensitivity of CFT was 71.0% with a specificity of 83.6%, while the sensitivity of ELISA-EB was 95.2% and the specificity was 54.2%. Furthermore, ELISAr-Chlamydia was the test with fewer false positives resulting from positive reactivity to C. pecorum, although 15% of the sera positive for C. pecorum but negative for C. abortus antibodies reacted positively. This study demonstrated with field material that ELISAr-Chlamydia provides the most balanced results between sensitivity and specificity, especially in flocks with no clinical OEA but reactivity to C. abortus.  相似文献   

Bovine viral diarrhoea virus in beef heifers in commercial herds in Australia: mob‐level seroprevalences and incidences of seroconversion,and vaccine efficacy     

JM Morton  NJ Phillips  LF Taylor  MR McGowan 《Australian veterinary journal》2013,91(12):517-524

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Squamous cell carcinoma in a tuatara (Sphenodon punctatus)     

WD Roe  MR Alley  SM Cooper  L Hazley 《New Zealand veterinary journal》2013,61(5):207-210

CASE HISTORY: A mature male tuatara was presented with a history of recurrent cloacal prolapse.

CLINICAL AND PATHOLOGICAL FINDINGS: The prolapsed tissue included a 12 mm diameter mass, which histologically showed cords and nodules of neoplastic epithelial cells.

DIAGNOSIS: The mass was diagnosed as a squamous cell carcinoma with ulceration of the overlying stratified squamous epithelium and diffuse inflammation of the surrounding dermis.

CLINICAL RELEVANCE: This case presented a rare opportunity to investigate squamous cell carcinoma in a long-lived lower vertebrate species. Future recurrence or metastasis of the mass may add useful information to the current base of knowledge of the behaviour of malignant neoplasms in reptiles.  相似文献   

Bacterial sinusitis as a cause of beak deformity in an Antipodes Island parakeet (Cyanoramphus unicolor)     

BD Gartrell  MR Alley  T Kelly 《New Zealand veterinary journal》2013,61(4):196-198

CASE HISTORY: From 26 days of age, an Antipodes Island parakeet (Cyanoramphus unicolor) was noted to have a severe beak deformity and reduced bodyweight gain compared to its nest mate. The bird was euthanised at 43 days of age.

CLINICAL AND PATHOLOGICAL FINDINGS: The beak abnormality consisted of distortion of the right nares and severe shortening resulting in deviation of the upper maxilla to the right and cranially. On sectioning the head, copious mucoid material was found in the infraorbital sinus and the bony sinus architecture was disrupted. Histopathological examination of the infraorbital sinuses revealed a large focus of chronic but active inflammation, bony lysis on the right side and pockets of a mixed population of bacteria.

DIAGNOSIS: Severe beak deformity, likely secondary to bacterial sinusitis.

CLINICAL RELEVANCE: The case illustrates the need to look for underlying aetiologies to beak malformation, particularly in young parrots.  相似文献