A BOOST TECHNIQUE FOR IRRADIATION OF MALIGNANT CANINE NASAL TUMORS     

Donald E.  Thrall  DVM  PHD  Margaret C.  McEntee  DVM†  Carol  Novotney  DVM†  Marlene L.  Hauck  DVM† Rodney L.  Page  DVM  MS† 《Veterinary radiology & ultrasound》1993,34(4):295-300

Eighteen dogs with malignant nasal cavity tumors were treated with radiation therapy, including a boost technique. Three 3:0 Gy boost doses were added to a treatment protocol consisting of sixteen 3.0 Gy daily fractions, bringing the total dose to 57 Gy. This boost technique was implemented without an associated increase in overall treatment time by giving the boost doses on a twice-a-day basis. Boost doses were given during the first half of the radiation therapy period. The treatment was completed as planned in 16 of the 18 dogs; two dogs received lower doses (51 and 54 Gy). Median survival was 177 days, poorer than in some other reported studies of nasal tumor irradiation. Acute effects were unacceptable, with 11 of the 18 dogs developing severe mucositis, desquamation, edema, swelling, and pruritus. The extensive nature of the acute reactions compromised assessment of the effect of the increased radiation dose on the tumor. Although there is justification for assessing more aggressive radiation protocols in canine nasal tumor patients, total doses approximating 60 Gy can not be given as described because of the inability of acutely responding normal tissues to compensate.  相似文献   

Thrombocytopenia in Cats: A Retrospective Study of 41 Cases     

Holly L. Jordan DVM    Carol B. Grindem DVM  PhD  Edward B. Breitschwerdt DVM 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1993,7(5):261-265

The prevalence of feline thrombocytopenia (<200,000 platelets/L) at North Carolina State University, College of Veterinary Medicine Teaching Hospital, from January 1985 to March 1990, was 1.2% (41/3300). Cats were divided into six categories based on clinical diagnoses: 29% (12/41) had infectious disease, 20% (8/41) had neoplasia, 7% (3/41) had cardiac disease, 2% (1/41) had primary immune-mediated disease, 22% (9/41) had multiple diseases, and 20% (8/41) had disorders of unknown etiology. The mean platelet count for all thrombocytopenic cats was 52,000/μL ± 46,000/μL (1 SD) with a range of 1000–190,000/μL. No significant differences were found between groups with respect to platelet count, packed cell volume, or white blood cell count, though anemia and leukopenia were common among the cats as a whole. Bleeding disorders (hemorrhage or thrombosis) were observed in 29% (12/41) of thrombocytopenic cats and were more likely to be associated with neoplasia, cardiac disease, and platelet counts less than or equal to 30,000/μL. Disseminated intravascular coagulopathy was diagnosed in 12% (5/41) of the cats. Infections and/or neoplasia affecting the bone marrow were the most common diseases associated with thrombocytopenia. Feline leukemia virus and myeloproliferative neoplasia accounted for approximately 44% (18/41) of the specific diagnoses in thrombocytopenic cats. (Journal of Veterinary Internal Medicine 1993; 7:261–265. Copyright © 1993 by the American College of Veterinary Internal Medicine.)  相似文献   

Wetland effects on lake water quality in the Minneapolis/St. Paul metropolitan area     

Naomi E. Detenbeck  Carol A. Johnston  Gerald J. Niemi 《Landscape Ecology》1993,8(1):39-61

A method developed to evaluate the cumulative effect of wetland mosaics on water quality was applied to 33 lake watersheds in the seven-county region surrounding Minneapolis-St. Paul, Minnesota. A geographic information system (GIS) was used to record and measure landscape variables derived from aerial photos. Twenty-seven watershed land-use and land-cover variables were reduced to eight principal components which described 85% of the variance among watersheds. Relationships between lake water quality variables and the first six principal components plus an index of lake mixis were analyzed through stepwise multiple regression analysis. A combination of three landscape components (wetland/watershed area, agriculture/wetlands, and forest/soils components) explained 49% of the variance in a trophic state index, even though most of the lakes examined were already highly eutrophic, and thus were influenced by internal loading. The regression equations explained a range of 14 to 76% of the variation in individual water quality variables. Forested land-use was associated with lower lake trophic state, chloride, and lead. High lake trophic state was associated with agricultural land-use and with wetland distance from the lake of interest. The extent of wetlands was associated with low total lead and high color in lakes downstream. Wet meadows or herbaceous, seasonally-flooded wetlands contributed more to lake water color than did cattail marshes.  相似文献   

Pharmacokinetic and Phase I Evaluation of Carboplatin in Dogs   总被引：1，自引：1，他引：0  

Rodney L. Page DVM  MS    Margaret C. McEntee DVM    Steven L. George PhD    Patrick L. Williams PhD    Greta L. Heidner DVM    Carol A. Novotney DVM    Jim E. Riviere DVM  PhD    Mark W. Dewhirst DVM  PhD  Donald E. Thrall DVM  PhD 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1993,7(4):235-240

Thirty dogs with spontaneously occurring malignant neoplasms were treated monthly with carboplatin (CBDCA) given as a 30-minute intravenous infusion in a dose escalation study. Twenty-eight dogs were considered evaluable for toxicity. The maximally tolerated dose of CBDCA was conceptually defined as that dose, determined by logistic regression analyses of toxicity data, resulting in a 50% incidence of moderate toxicity (MOD₅₀) or a 5% incidence of severe toxicity (SEV₅). Each designated maximally tolerated dose was calculated for the first course of treatment only and for the first and second courses of treatment combined to estimate cumulative drug toxicity. The MOD₅₀ and SEV₅ for the first treatment course were 340 and 278 mg/M², respectively. MOD₅₀ and SEV₅ values for the first plus second treatment courses were 327 and 231 mg/M², respectively. The nadir of neutrophil and platelet counts occurred approximately 14 days after treatment. The mean neutrophil and platelet values for all dogs experiencing myelosuppression during the first two treatment courses were 1541/μL and 62,600/μL, respectively. Nonparametric pharmacokinetic analysis of plasma CBDCA values suggested that half-life (T_1/2), area-under-the-curve and total body clearance (CL_b) were not dose dependent. Volume of distribution (VD_ss) significantly increased with dose only between 100 and 150 mg/M², not between 150 and 300 mg/M². Dose-independent serum CBDCA pharmacokinetic disposition indicates that detailed investigation of tissue CBDCA distribution would be warranted and may identify novel dosing strategies that could improve the therapeutic index of CBDCA by minimizing toxicity. (Journal of Veterinary Internal Medicine 1993; 7:235–240. Copyright © 1993 by the American College of Veterinary Internal Medicine.)  相似文献   

Proposed criteria for classification of acute myeloid leukemia in dogs and cats     

Jain NC  Blue JT  Grindem CB  Harvey JW  Kociba GJ  Krehbiel JD  Latimer KS  Raskin RE  Thrall MA  Zinkl JG 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1991,20(3):63-82

Blood and bone marrow smears from 49 dogs and cats, believed to have myeloproliferative disorders (MPD), were examined by a panel of 10 clinical pathologists to develop proposals for classification of acute myeloid leukemia (AML) in these species. French-American-British (FAB) group and National Cancer Institute (NCI) workshop definitions and criteria developed for classification of AML in humans were adapted. Major modifications entailed revision of definitions of blast cells as applied to the dog and cat, broadening the scope of leukemia classification, and making provisions for differentiating erythremic myelosis and undifferentiated MPD. A consensus cytomorphologic diagnosis was reached in 39 (79.6%) cases comprising 26 of AML, 10 of myelodysplastic syndrome (MDS), and 3 of acute lymphoblastic leukemia (ALL). Diagnostic concordance for these diseases varied from 60 to 81% (mean 73.3 +/- 7.1%) and interobserver agreement ranged from 51.3 to 84.6% (mean 73.1 +/- 9.3%). Various subtypes of AML identified included Ml, M2, M4, M5a, M5b, and M6. Acute undifferentiated leukemia (AUL) was recognized as a specific entity. M3 was not encountered, but this subclass was retained as a diagnostic possibility. The designations M6Er and MDS-Er were introduced where the suffix "Er" indicated preponderance of erythroid component. Chief hematologic abnormalities included circulating blast cells in 98% of the cases, with 36.7% cases having >30% blast cells, and thrombocytopenia and anemia in approximately 86 to 88% of the cases. Bone marrow examination revealed panmyeloid dysplastic changes, particularly variable numbers of megaloblastoid rubriblasts and rubricytes in all AML subtypes and increased numbers of eosinophils in MDS. Cytochemical patterns of neutrophilic markers were evident in most cases of Ml and M2, while monocytic markers were primarily seen in M5a and M5b cases. It is proposed that well-prepared, Romanowsky-stained blood and bone marrow smears should be examined to determine blast cell types and percentages for cytomorphologic diagnosis of AML. Carefully selected areas of stained films presenting adequate cellular details should be used to count a minimum of 200 cells. In cases with borderline diagnosis, at least 500 cells should be counted. The identity of blast cells should be ascertained using appropriate cytochemical markers of neutrophilic, monocytic, and megakaryocytic differentiation. A blast cell count of > 30% in blood and/or bone marrow indicates AML or AUL, while a count of < 30% blasts in bone marrow suggests MDS, chronic myeloid leukemias, or even a leukemoid reaction. Myeloblasts, monoblasts, and megakaryoblasts comprise the blast cell count. The FAB approach with additional criteria should be used to distinguish AUL and various subtypes of AML (Ml to M7 and M6Er) and to differentiate MDS, MDS-ER, chronic myeloid leukemias, and leukemoid reaction. Bone marrow core biopsy and electron microscopy may be required to confirm the specific diagnosis. Immunophenotyping with lineage specific antibodies is in its infancy in veterinary medicine. Development of this technique is encouraged to establish an undisputed identity of blast cells. Validity of the proposed criteria needs to be substantiated in large prospective and retrospective studies. Similarly, clinical relevance of cytomorphologic, cytochemical, and immunophenotypic characterizations of AML in dogs and cats remains to be determined.  相似文献   

What Is Your Diagnosis?     

Jordan HL  Sellon RK  Laws TA  Tompkins WA  Grindem CB 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1994,23(4):131-134

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The effect of in vitro antibacterial properties of bovine milk diets on the natural colonisation of newborn piglets with coliform bacteria.     

S Stephens  M John  J E Cooper 《Research in veterinary science》1979,26(1):21-24

The diets suitable for the hand rearing of piglets in incubators were examined. Diet A was based on cows' milk heated to 56 degrees C and had bacteriostatic and antiadhesive properties against Escherichia coli. Diet B was based on evaporated cows' milk and did not have these properties. The numbers of coliform bacteria naturally colonising the small intestines of newborn piglets fed entirely on these diets for one week did not differ significantly, however both were significantly higher than in control piglets suckled from birth. Faecal counts of coliforms were similar in all three groups. Examination of the bacteriostatic sensitivity of the isolated strains to sows' milk indicated a predominance of milk-sensitive strains colonising the suckled piglets and a predominance of milk-resistant strains in the piglets fed diet B. This diet-dependent colonisation could not be explained by the in vitro bacteriostatic properties of the diets alone.  相似文献   

Transport media for Campylobacter fetus venerealis     

de Lisle GW  Stephens DJ  Bird MM 《New Zealand veterinary journal》1982,30(3):31-32

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Application of nox-restriction fragment length polymorphism for the differentiation of Brachyspira intestinal spirochetes isolated from pigs and poultry in Australia.     

Kirsty M Townsend  Vo Ngan Giang  Carol Stephens  Paul T Scott  Darren J Trott 《Journal of veterinary diagnostic investigation》2005,17(2):103-109

Sixty-nine intestinal spirochetes isolated from pigs and poultry in eastern Australia were selected to evaluate the effectiveness of a species-specific PCR-based restriction fragment length polymorphism (RFLP) analysis of the Brachyspira nox gene. For comparative purposes, all isolates were subjected to species-specific PCRs for the pathogenic species Brachyspira hyodysenteriae and Brachyspira pilosicoli, and selected isolates were examined further by sequence analysis of the nox and 16S ribosomal RNA genes. Modifications to the original nox-RFLP method included direct inoculation of bacterial cells into the amplification mixture and purification of the PCR product, which further optimized the nox-RFLP for use in a veterinary diagnostic laboratory, producing sufficient product for both species identification and future comparisons. Although some novel profiles that prevented definitive identification were observed, the nox-RFLP method successfully classified 45 of 51 (88%) porcine and 15 of 18 (83%) avian isolates into 5 of the 6 recognized species of Brachyspira. This protocol represents a significant improvement over conventional methods currently used in veterinary diagnostic laboratories for rapid specific identification of Brachyspira spp. isolated from both pigs and poultry.  相似文献   

Water requirements of canine athletes during multi‐day exercise          下载免费PDF全文

Lara Stephens‐Brown  Michael Davis 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(3):1149-1154

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