Detection and characterization of leptospiral antigens using a biotin/avidin double-antibody sandwich enzyme-linked immunosorbent assay and immunoblot.   总被引：2，自引：1，他引：1       下载免费PDF全文

M J Champagne  R Higgins  J M Fairbrother    D Dubreuil 《Canadian journal of veterinary research》1991,55(3):239-245

A biotin/avidin double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of antigens of Leptospira interrogans serovars in experimentally inoculated bovine urine samples was evaluated. Immunoglobulin G (IgG) from rabbits immunized with L. interrogans serovar hardjo type hardjobovis sonicated, whole cell, and formalinized-heated antigen preparations were purified by a protein A-superose column coupled to fast protein liquid chromatography, and evaluated for species specificity in the ELISA. The ELISA using each specific IgG detected as few as 10(4) leptospires of the homologous serovar hardjo diluted in phosphate-buffered saline solution with Tween 20 (PBSS-Tween 20). On immunoblot analysis of proteinase-K-digested whole cell leptospiral preparations, each IgG revealed the presence of bands specific to serovar hardjo, suggesting the presence of serovar-specific epitopes on the lipopolysaccharide molecules. The minimum number of cells of heterologous serovars pomona, grippotyphosa, bratislava, icterohaemorrhagiae and copenhageni detected by each ELISA was greater, ranging from 10(6) to 10(7). The common antigenic determinants observed on immunoblot analysis were different for each specific IgG, except for a major cross-reacting, possibly flagellar, protein doublet at approximately 36-36.5 kDa. Leptospires were equally well detected by the ELISA in both bovine urine and PBSS-Tween 20.  相似文献   

Pronuclear Embryo Yield in Canindé and Saanen Goats for DNA Microinjection     

RR Moura  ES Lopes‐Junior  DIA Teixeira  IA Serova  LE Andreeva  LM Melo  VJF Freitas 《Reproduction in domestic animals》2010,45(5):e101-e106

The objective of this study was to examine the effect of donor breed on pronuclear‐stage embryo yield to be used for DNA microinjection in a transgenesis goat program. Twelve Canindé and twelve Saanen goats were heat synchronized using a progestagen‐cloprostenol treatment. Forty‐eight hours before the sponge removal, superovulation was induced with a total administration of 4.4 mg/kg bodyweight NIH‐FSH‐P1, given twice daily in decreasing doses over 3 days. In addition, goats received 100 μg of GnRH and they were hand‐mated at 36 and 48 h after progestagen removal. Embryo recovery was performed by oviduct flushing at 72 h after sponge removal. Embryos were microinjected with a DNA construct and noticeable swelling of the nuclei was the criterion for successful microinjection. The total diameter, cytoplasm diameter, zona pellucida thickness and pronuclei diameter were measured for each microinjected embryo. A higher (p < 0.05) percentage of fertilized ova was observed in Canindé (89.9%) than Saanen (36.2%) goats. In addition, Canindé donors produced a higher percentage of pronuclear embryos when compared with Saanen: 72.5% vs 20.6% (p < 0.05), respectively. Successful microinjection was verified in 96.7% and 73.3% of times in Canindé and Saanen embryos, respectively (p < 0.05). Significant differences were observed for all morphometric parameters except for cytoplasm diameter. In conclusion, under our study experimental conditions, Canindé were more efficient than Saanen goats concerning the pronuclear embryo yield and manipulation. The use of Canindé goats in transgenesis programs could be increase the interest in their breeding and could be contribute to saving them from extinction.  相似文献   

Intraosseous approach to the nasolacrimal duct for removal of a foreign body in a dog     

Pope ER  Champagne ES  Fox D 《Journal of the American Veterinary Medical Association》2001,218(4):541-2, 526

A Labrador retriever was evaluated because of chronic mucopurulent discharge from the left eye. A foreign body was identified in the nasolacrimal duct by use of dacryocystorhinography. Attempts to alleviate the inflammation by use of flushing and administration of antimicrobials were unsuccessful. At surgery, the infraorbital foramen was used as a landmark for a skin incision, because the nasolacrimal duct courses dorsal and parallel to the infraorbital canal. An air drill was used to remove the portion of the maxillary bone overlying the nasolacrimal duct, which exposed the intraosseous portion of the duct and allowed removal of a plant-material foreign body. The incision in the duct was allowed to heal by second intention, and the dog recovered without complications.  相似文献   

Equine conjunctival pseudotumors     

Moore CP  Grevan VL  Champagne ES  Collins BK  Collier LL 《Veterinary ophthalmology》2000,3(2-3):57-63

Five horses presented with unilateral pink, smooth, nonulcerated conjunctival masses with histologic features characteristic of inflammatory pseudotumors, i.e. proliferative inflammatory lesions clinically resembling true neoplasia. Although causes for the inflammatory lesions were not determined, based on the presence histologically of mononuclear (predominantly lymphocytic) inflammatory cell infiltrates and the absence of infectious agents, parasites or foreign bodies, an immune-mediated pathogenesis was suspected. Affected horses ranged from 5 to 8 years of age with no apparent breed or sex predilection. Conjunctival lesions were nodular in two cases and relatively flat and more diffuse in three cases. Third eyelid lesions were present in three cases and two affected eyes had corneal involvement. Based on findings from these five cases, the prognosis for equine conjunctival pseudotumors appears to be good when lesions are treated by partial or complete surgical excision, local administration of anti-inflammatory agents, or a combination of surgery and anti-inflammatory therapy.  相似文献   

Primary lens luxation in the Chinese Shar Pei: clinical and hereditary characteristics     

Lazarus JA  Pickett JP  Champagne ES 《Veterinary ophthalmology》1998,1(2-3):101-107

A pedigree analysis of a family of 15 related Chinese Shar Peis was conducted. This pedigree analysis, including affected and nonaffected dams, sires and offspring, was compiled to document and characterize the occurrence, common clinical signs, and age of onset of primary lens luxation while suggesting a possible mode of inheritance in this breed. Of the five offspring from the mating of an affected dam to two unrelated affected males, 100% of offspring were affected with bilateral primary lens luxations. Of the four viable offspring from the mating of the same affected dam to an unrelated, unaffected male, two dogs (50%) were affected. The average age of onset of affected animals (seven) in this first generation was 4.9 years (range 3–6 years). The six dogs in the second generation of the same pedigree line were 2-years-old at examination with none of these animals affected at the time of this study. The most common presenting complaints were a unilateral change in ocular appearance (5 of 7 dogs) and subjective vision impairment (4 of 7 dogs). The most common clinical sign upon ophthalmic examination was iridodonesis (unilateral 4 of 7 dogs; bilateral 3 of 7 dogs) and the presence of an aphakic crescent (3 of 7 dogs). Gonioscopy and tonometry of severely affected eyes revealed a narrow or closed iridocorneal angle and ocular hypertension. This study suggests that primary lens luxation does occur in the Chinese Shar Pei, resembling the clinical condition (age of onset, clinical signs) previously described in the terrier breeds, the Border Collie, and the Tibetan Terrier. Application of the phenotypic findings in this study to a Mendelian genetic model of inheritance suggests that primary lens luxation in the Chinese Shar Pei is inherited as a simple autosomal recessive trait.  相似文献   

Effects of an extreme flash flood on the native fish assemblages across a Mediterranean catchment     

A. M. PIRES  M. F. MAGALHÃES  L. MOREIRA DA COSTA  M. J. ALVES  & M. M. COELHO 《Fisheries Management and Ecology》2008,15(1):49-58

Abstract  The effects of an extreme flash flood on the native fish assemblages across a Mediterranean catchment in Southwest Portugal were investigated. The fish community structure and habitat were surveyed at 10 sites pre- and post-flood. Overall species richness and rank abundances changed little after the flood, despite there being a consistent decline in the abundance of small cyprinids. Pre- and post-flood habitat correlates of abundance changed for at least some species, and variability in assemblage structure was associated with flood, depth and pH. These results indicate that extreme floods occurring early in the wet season may have little disruptive effects on the overall structure of native fish assemblages in Mediterranean streams, although may at least partially influence population dynamics for some species. Habitat quality and complexity are likely to play a role in mediating species responses to flooding.  相似文献   

Clinical diagnosis of uterine didelphia in Ayrshire heifer     

Raggio I  Lefebvre RC  Poitras P  Vaillancourt D  Champagne JP 《The Canadian veterinary journal. La revue veterinaire canadienne》2006,47(9):899-901

A 24-month-old Ayrshire heifer was referred because of infertility. A clinical diagnosis of uterus didelphys was established after a complete genital examination before the animal was culled from the herd.  相似文献   

Biofortification of taro (Colocasia esculenta) through breeding for increased contents in carotenoids and anthocyanins     

A. Champagne  L. Legendre  V. Lebot 《Euphytica》2013,194(1):125-136

Biofortification of taro (Colocasia esculenta) has never been studied. The aim of the present study is to compare the chemical compositions and individual constituent contents for major compounds (starch, sugars, cellulose, proteins, minerals), carotenoids and anthocyanins between parents and hybrids selected first for their agronomic performance and second for their corm characteristics (flesh color, quality and taste). For major compounds, 45 selected hybrids were compared to 66 cultivars and for carotenoids and anthocyanins, 34 selected hybrids were compared to 79 cultivars. Total sugars, cellulose and mineral contents presented moderate increases in hybrids. Carotenoids and anthocyanins contents were not correlated with corm flesh colors, most likely in relation with the wide range of the observed variability. Anthocyanin contents could not be increased in the selected hybrids, while total carotenoid contents were increased by more than fourfolds. The results of this study indicate that carotenoid contents can be rapidly improved by selecting plants of good agronomic performance and corm shape with increased density of yellow and orange colors. Potential applications to taro breeding programs are discussed.  相似文献   

Unconditional quantum teleportation   总被引：5，自引：0，他引：5  

A Furusawa  JL Sorensen  SL Braunstein  CA Fuchs  HJ Kimble  ES Polzik 《Science (New York, N.Y.)》1998,282(5389):706-709

Quantum teleportation of optical coherent states was demonstrated experimentally using squeezed-state entanglement. The quantum nature of the achieved teleportation was verified by the experimentally determined fidelity Fexp = 0.58 +/- 0.02, which describes the match between input and output states. A fidelity greater than 0.5 is not possible for coherent states without the use of entanglement. This is the first realization of unconditional quantum teleportation where every state entering the device is actually teleported.  相似文献   

Two‐Dimensional Vibration Spectroscopy of Rice Quality and Cooking     

F. E. Barton  D. S. Himmelsbach  A. M. McClung  E. L. Champagne 《Cereal Chemistry》2002,79(1):143-147

Rice samples were taken from a study of rice milling properties that affect quality. The spectra of milled and cooked samples were taken in the near‐infrared, mid‐infrared, and Raman region. These spectra, two regions at a time, were regressed by a two‐dimensional technique to develop contour maps that indicated the correlation of two spectral regions. These relationships demonstrate that it is possible to recognize the hydration effects caused by gelatinization (cooked samples vs. milled rice). Three water (O‐H stretch) spectral bands (960, 1445, 1,930 nm) in the near‐infrared (NIR) show marked differences between milled and cooked rice. The difference spectra indicated that there were additional phenomena occurring besides the addition of water. These differences are apparent in both C‐O‐H and N‐H bands, which indicate that water is interacting with both starch and protein. The two‐dimensional technique developed in this laboratory was used to get a better interpretation of what occurs during cooking. The Raman spectrum, which is relatively insensitive to water (O‐H stretch), revealed only changes in protein that could be associated with denaturization.  相似文献