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Koji?YoshidaEmail author Ryota?Shibasaki Chika?Takami Chisato?Takenaka Kazukiyo?Yamamoto Takafumi?Tezuka 《Journal of Forest Research》2004,9(3):195-203
In order to clarify the chronic influence of acid fog on the gas exchange rates of momi fir (Abies firma Sieb. et Zucc.) trees, we exposed them to simulated acid fog (pH 3) for 3 years. The composition of the acid fog was similar to that observed in a region where momi fir trees have been declining, and it contained organic acids. We then treated the firs with various additional stresses, such as drought, low temperature, fine root cutting, ozone exposure, soil acidification, nitrogen load, and rhizosphere aluminum stress. Under chronic exposure to acid fog, the momi fir seedlings exhibited a pattern of stomatal behavior whereby they excessively opened in summer and closed in winter. Furthermore, the stomata of these seedlings tended to open during drought stress, and their needles were visibly injured after ozone exposure. The net photosynthesis rates of the seedlings exposed to acid fog were regulated by their stomatal aperture, rather than directly by acid fog. These results suggest that acid fog exposure disturbs the control of stomatal function in the momi fir seedlings. In addition, we found that chronic acid fog exposure suppressed the decrease in net photosynthesis rate, due to its nitrogen load. 相似文献
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Nobuaki Tamai Chisato Takenaka Shigehiro Ishizuka Takafumi Tezuka 《Journal of Forest Research》2001,6(4):311-313
The relationship between soil air volume and CH4 consumption in brown forest soils was investigated. The CH4 consumption was negatively affected when the soil air volume decreased in experiments carried out bothin situ and in the laboratory. A strong dependence of the CH4 consumption on the soil air volume was found in the laboratory experiment. These results indicate the possibility that the
soil air volume is the primary rate-limiting factor for the CH4 consumption in forest soil. Thus our work suggests that the other factors by which CH4 consumption might be affected should be examined under the same air volume condition in the soil. To obtain accurate data,
we need to be careful not to change the air volume by trampling or disturbing during the measurement of the natural methane
fluxin situ. 相似文献
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This article discusses the effect of aluminum on root growth of Querucus serrata Thunb. In a 9-week hydroponics experiment, the effects of various concentrations (0 to 5.0 mM) of Al on root growth were examined. Results revealed that root biomass increases with the increase in Al concentration up to 2.5 mM, and then it tended to decrease. In the next experiment, the effects of H+ and Al3+ on roots were compared in a 4-week hydroponics experiment using three treatment solutions: control (pH 6.0), −Al (without Al, pH 3.5), and +Al (with 2.5 mM Al, pH 3.5). No clear difference in the biomass and root length between the control and −Al treated roots was observed, and root and shoot biomass were increased by Al treatment. These results confirmed that the H+ concentration level, at a pH of 3.5, is not toxic for Q. serrata and the Al-induced increase in root biomass is not caused by the amelioration of H+ toxicity by Al. In the third experiment, roots were exposed to an Al solution (pH 3.6) intermittently. This treatment clarified that Al stimulated rooting and root elongation. In the fourth experiment, the effect of 1 mM Al on root growth during a 15-month period in a sand culture were examined. This experiment confirmed that Al stimulated good growth and development of root systems at appropriate concentrations. Therefore, it is considered that Al-induced root growth enhancement occurs as a long-term and short-term phenomenon. 相似文献
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Koji Yoshida Shoko Inaba Chisato Takenaka Takafumi Tezuka 《Journal of Forest Research》2005,10(2):157-161
To clarify the nutrient status in momi fir (Abies firma Sieb. et Zucc.) seedlings under complex stress conditions of acid fog with soil acidification or nitrogen loading, we exposed seedling shoots to simulated acid fog (pH 3) and simultaneously loaded the soil with acid or excess nitrogen for 17 months. Proton and nitrogen loading reduced K concentration in soil, but these treatments had little effect on the nutrient status of fine roots in momi fir seedlings. Acid fog exposure resulted in reduced concentrations of Mg, Al, and Fe in current-year needles and Mg, Al, Cu, Fe, and Zn in 1-year-old needles. The complex effects of acid fog exposure with proton or nitrogen loading on nutrient status were relatively slight. However, elements such as Al and Cu notably reflect the effects of proton loading. These results show that the nutrient status of trace elements, rather than major elements, in needles of momi fir seedlings is sensitive to exposure to acid fog or proton loading. 相似文献
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Chisato YONEMOCHI Takuo IKEDA Chisato HARADA Toyoko KUSAMA Michito HANAZUMI 《Animal Science Journal》2003,74(2):81-88
The influence of transgenic event CBH 351 (Starlink)-derived hybrid corn (SL) on the health condition, physiological function and lactational performance of dairy cows as well as the transfer of the cry9C gene and Cry9C protein present in SL to milk, blood, liver and muscles was examined, and compared with a diet containing non-transgenic (isogenic) control corn (non-SL). After adapting to a diet containing non-SL for 2 weeks, four Holstein cows were assigned to each of the non-SL and SL groups and were fed diets containing non-SL or SL, respectively, for 5 weeks. There were no significant influences on the physiological condition, milk yield or serum biochemical and hematological values after feeding with SL. There was also no influence on pH value, cell density of protozoa, or volatile fatty acid concentration and composition of rumen fluids. In addition, no significant differences were observed on histopathological examination of the major organs and tissues between the SL and non-SL groups. Moreover, the cry9C gene and Cry9C protein were not detected by the polymerase chain reaction method and ELISA in the milk, blood, liver and muscles of the cows at the end of the experiment. 相似文献
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The consumption of poultry meats exceeds 50% of total meat consumption. With the outbreak of bovine spongiform encephalopathy and foot and mouth disease, the consumption of poultry meat is set to increase. The use of meat bone meal derived from cattle and pigs is prohibited. Therefore, plant protein has to be used more frequently and the importance of the study of amino acid nutrition is increasing. In order to improve dietary efficiency, it is necessary to elucidate the exact amino acid requirements of poultry. The amino acid requirements are not constant and are affected by various factors. By using the plasma amino acid concentration as a parameter, it is possible to predict the amino acid requirements within a short period using a small number of animals repeatedly. By satisfying the amino acid requirements through supplementing crystalline amino acids, it is possible to reduce dietary protein and excretory nitrogen simultaneously. However, when the dietary protein is reduced, the abdominal fat tends to increase. This is solved by deciding the optimum dietary protein level, but it is difficult to control the taste of poultry meat by dietary amino acids. 相似文献
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Nakamura K Murata C Ito M Iwamori T Nishimura S Hisamatsu S Sonoki S Nakayama A Suyama E Kawasaki H Taira K Nishino K Tachi C 《The Journal of reproduction and development》2006,52(1):73-80
As the first step in investigating the possiblity of applying ribozyme technology to artificial control of the sex ratios at birth in farm animals, where the demand for females exceeds that for males, we designed a hammerhead ribozyme (HHRz) and 2 tRNA(val)-hammerhead ribozyme complexes (tRNARz3 and tRNARz4), and examined their effects upon murine Sry mRNA in vitro and in cells. We demonstrated that HHRz and tRNARz3 could effectively cleave the target Sry mRNA in vitro. For the purpose of experiments in vivo, HHRz was cloned into the highly efficient pUC-CAGGS mammalian expression vector (pCAG/HHRz), and the tRNA ribozyme complexes were cloned into the pol III promoter-driven pPUR-KE vector (pPUR/tRNARz3 and pPUR/tRNARz4); the ribozyme vectors were co-transfected with the target vector (pCAG/Sry). A suppressive action (up to approx. 60%) was confirmed for pCAG/HHRz and pPUR/tRNARz3 upon the transiently expressed exogenously introduced Sry in M15 cultured cells. 相似文献
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Chisato Tomimura B. M. P. Singhakumara P. Mark S. Ashton 《Journal of Sustainable Forestry》2013,32(8):818-834
This study examined the vegetation growing beneath Caribbean pine (Pinus caribaea) plantations established within previously abandoned tea lands adjacent to intact rain forest in southwest Sri Lanka. To examine the pattern and composition of secondary succession and factors affecting it, vegetation was sampled in different relative locations (interior and edge conditions) within plantations. Results demonstrated that more vegetation was found near the plantation edges than in the interior, and this pattern was prominent for both wind- and bird- dispersed species. The vegetation represented a mixture of species belonging to a range of successional guilds representing early (17 species, 42.4% in stem count) and late-successional species (52 species, 40.6%), although native long-lived canopy tree species were mostly absent. Bird-dispersed species dominated the flora (80 species, 86.7% in stem count). Abundance of an exotic shrub Clidemia hirta (Melastomataceae) showed a negative correlation with that of other species, indicating its impact on native flora. Underplanting of native canopy species may be effective in assisting secondary succession and control C. hirta in the plantations. 相似文献
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Denny Irawati Yuya Takashima Chisato Ueda J. P. Gentur Sutapa Sri Nugroho Marsoem Futoshi Ishiguri Kazuya Iizuka Nobuo Yoshizawa Shinso Yokota 《Journal of Wood Science》2013,59(6):522-527
We examined the enzymatic saccharification and ethanol fermentation of the spent media (SMs) from Auricularia polytricha cultivation using wood meals of Falcataria moluccana, Shorea sp., and Tectona grandis. Although the hydrolysis weight decrease and reducing sugar yield were higher in SM of F. moluccana, the ethanol yield was higher in SM of Shorea sp. Ozone treatment of SM further increased the hydrolysis weight decrease, reducing sugar, and ethanol yields in Shorea sp. These results indicate that SM of A. polytricha is a suitable biomass material to produce fermentable sugars for ethanol production, and that ozone treatment is a suitable method for increasing the ethanol yield. 相似文献