Immunocytochemical Study of the Ultimobranchial Tubule in Wistar Rats     

E. Conde    A. M. Moreno    I. Martin-Lacave  A. Fernandez y H.  Galera 《Anatomia, histologia, embryologia》1992,21(1):94-100

A systematic immunohistochemical study of the ultimobranchial tubule (UBT) has been carried out in 45 Wistar rats of different ages (0, 5, 10, 15, 20, 25, 30, 60 and 120 days). The existence of calcitonin immunoreactive cells in the UBT wall has been demonstrated in a 5-days old rat. In addition, immunohistochemical studies for thyroglobulin revealed positive staining in follicular cells connected to the UBT and, occasionally, in isolated cells lying within solid clusters from the UBT. These last results together with the continued and repeated existence of numerous mitosis and PAS (+) microfollicles, apparently rising from the UBT, support the hypothesis that the ultimobranchial body (UBB) may contribute partially to the formation of a part of the follicular component.  相似文献   

Potential Anti-Obesity,Anti-Steatosis,and Anti-Inflammatory Properties of Extracts from the Microalgae Chlorella vulgaris and Chlorococcum amblystomatis under Different Growth Conditions     

Ana Regueiras  lvaro Huguet  Tiago Conde  Daniela Couto  Pedro Domingues  Maria Rosrio Domingues  Ana Margarida Costa  Joana Laranjeira da Silva  Vitor Vasconcelos  Ralph Urbatzka 《Marine drugs》2022,20(1)

Microalgae are known as a producer of proteins and lipids, but also of valuable compounds for human health benefits (e.g., polyunsaturated fatty acids (PUFAs); minerals, vitamins, or other compounds). The overall objective of this research was to prospect novel products, such as nutraceuticals from microalgae, for application in human health, particularly for metabolic diseases. Chlorella vulgaris and Chlorococcum amblystomatis were grown autotrophically, and C. vulgaris was additionally grown heterotrophically. Microalgae biomass was extracted using organic solvents (dichloromethane, ethanol, ethanol with ultrasound-assisted extraction). Those extracts were evaluated for their bioactivities, toxicity, and metabolite profile. Some of the extracts reduced the neutral lipid content using the zebrafish larvae fat metabolism assay, reduced lipid accumulation in fatty-acid-overloaded HepG2 liver cells, or decreased the LPS-induced inflammation reaction in RAW264.7 macrophages. Toxicity was not observed in the MTT assay in vitro or by the appearance of lethality or malformations in zebrafish larvae in vivo. Differences in metabolite profiles of microalgae extracts obtained by UPLC-LC-MS/MS and GNPS analyses revealed unique compounds in the active extracts, whose majority did not have a match in mass spectrometry databases and could be potentially novel compounds. In conclusion, microalgae extracts demonstrated anti-obesity, anti-steatosis, and anti-inflammatory activities and could be valuable resources for developing future nutraceuticals. In particular, the ultrasound-assisted ethanolic extract of the heterotrophic C. vulgaris significantly enhanced the anti-obesity activity and demonstrated that the alteration of culture conditions is a valuable approach to increase the production of high-value compounds.  相似文献   

Ontogenetic movements of juvenile blacktip reef sharks: evidence of dispersal and connectivity between coastal habitats and coral reefs     

A Chin  MR Heupel  CA Simpfendorfer  AJ Tobin 《水产资源保护:海洋与淡水生态系统》2013,23(3):468-474

Retracted: Effect of Post‐Thaw Storage Time on Motility and Fertility of Cryopreserved Beluga Sturgeon (Huso huso) Sperm          下载免费PDF全文

MS Aramli  RM Nazari  MR Gharibi 《Reproduction in domestic animals》2015,50(2):349-352

The aim of this study was to test the influence of post ‐ thaw storage time on the duration of sperm motility, percentage of motile sperm, and fertilization and hatching rates of fresh sperm and sperm stored for 0, 30 and 60 min at 4°C post‐thawing. After being frozen in liquid nitrogen and then thawed, the percentage of motile sperm and duration of motility were not affected by 30 min of storage at 4°C, whereas a significant decline in these parameters was observed after 60 min of storage. Similarly, fertilization and hatching rates were significantly affected within 60 min of storage at 4°C, and the fertility of frozen‐thawed sperm was significantly lower than that of fresh sperm. We conclude that cryopreserved sperm of beluga sturgeon could be stored for 30 min without the loss of sperm quality. This described procedure for beluga sturgeon cryopreservation is reliable and efficient and therefore can be recommended for hatchery practice after scaling up this technique.  相似文献   

The Influence of Morphology,Follicle Size and Bcl‐2 and Bax Transcripts on the Developmental Competence of Bovine Oocytes     

THC De Bem  PR Adona  FF Bressan  LG Mesquita  MR Chiaratti  FV Meirelles  CLV Leal 《Reproduction in domestic animals》2014,49(4):576-583

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Recovery of bioactive compounds from Pinus pinaster wood by consecutive extraction stages     

Enma Conde  Wenwen Fang  Jarl Hemming  Stefan Willför  Herminia Domínguez  Juan Carlos Parajó 《Wood Science and Technology》2014,48(2):311-323

Pinus pinaster wood samples, obtained at different positions of three healthy trees, were subjected to two sequential extractions using an Accelerated Solvent Extraction instrument. The first extraction was carried out with hexane (to remove lipophilic extractives) and the second one with acetone/water (95:5 v/v) to recover bioactive phenolic compounds, the target compounds of this study. The extracted fractions were assayed for total yield and composition. The extracts contained a spectrum of phenolic compounds (simple phenolics, phenolic stilbenes, flavonoids and lignans) and non-phenolic components (juvabiones, resin and fatty acids, steryl esters and triglycerides). The fractionation effects achieved by consecutive extractions and the recovery of bioactive phenolics are discussed.  相似文献   

Rhodococcus equi-associated osteomyelitis in foals     

EC FIRTH  MR ALLEY†  H HODGE 《Australian veterinary journal》1993,70(8):304-307

Two cases of Rhodococcus equl infection in foals are described, in which osteomyelitis was a feature. Because rhodococcal infection is usually low grade and chronic, and because the signs of early metaphysitis can be subtle, any articular or periarticular swelling in a foal from a farm with a history of rhodococcosis should be strongly suspected to be associated with R equl until proven otherwise.  相似文献   

Reply     

MR ALDERTON 《Australian veterinary journal》1993,70(11):429-430

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Analysis of IgG response to experimental infection with RH Toxoplasma gondii in goats.     

M Conde  J M Caballero  E Rodríguez-Ponce  A Ruiz  J González 《Comparative immunology, microbiology and infectious diseases》2001,24(3):197-206

The IgG response of goats experimentally infected with RH Toxoplasma gondii has been analysed using an indirect ELISA and Western-blot analysis. Specific IgG antibodies were first detected at 14 days post-inoculation (p.i.), reaching a peak by day 35 p.i. and showing slight fluctuations until the end of the experiment (91 p.i.). Specific IgG showed a reactivity over a whole range of peptides (125-24 kDa approximately), but the highest reactivity was observed against a group of antigens with a molecular weight between 34 and 28 kDa, in particular against a 30 kDa fraction which is considered to represent the major surface protein of T. gondii named p30 or SAG-1.  相似文献   

Species diversity: Concept,measurement, and response to clearcutting and site-preparation     

Benee F. Swindel  Louis F. Conde  Joel E. Smith 《Forest Ecology and Management》1984,8(1):11-22

A review of aspects of diversity recently introduced in the statistical literature by Patil and Tallie is given: species diversity as an average property of a community is defined; the property averaged is identified as species rarity; and relations between common indices of diversity and associated measures of rarity are illuminated. This paper also computes common indices of diversity from substantial empirical data collected before clearcutting and site-preparation under two management regimes and for 3 years following planting of slash pine (Pinus elliottii) to illustrate that the initial response to these forest operations is increased species diversity, i.e., increased species rarity. This response may be expected whenever clearcutting and site preparation reduces but does not eliminate previously abundant, late-successional species and permits the reappearance of early-successional species, including forbs and grasses.  相似文献