In vitro studies of Norwegian Red bovine semen immobilized and cryopreserved in alginate solid gel network          下载免费PDF全文

AH Alm‐Kristiansen  ER Gaustad  G Bai  FB Standerholen  G Klinkenberg  E Kommisrud  KE Waterhouse 《Reproduction in domestic animals》2018,53(2):365-370

Development of new semen cryopreservation techniques improving sperm survival and ensuring availability of viable spermatozoa for a prolonged time‐period after AI is promising tools to reduce sensitivity of timing of AI and enhance overall fertility. The SpermVital^® technology utilizes immobilization of bull spermatozoa in a solid network of alginate gel prior to freezing, which will provide a gradual release of spermatozoa after AI. The objective of this study was to compare post‐thaw sperm quality and in vitro sperm survival over time of Norwegian Red bull semen processed by the SpermVital^® (SV) technology, the first commercialized production line of SpermVital^® (C) and by conventional procedure applying Biladyl^® extender (B). Post‐thaw sperm motility was not significantly different between SV, C and B semen (p > .05). However, sperm viability and acrosome intactness were higher for SV than C and B semen (p < .05). Small differences in DNA quality were observed (p < .05). Sperm viability after storage in uterus ex vivo was higher for SV than for C semen (p < .05). Furthermore, sperm survival in vitro over time at physiological temperature was significantly higher for SV semen than C semen as well as B semen during the incubation period of 48 hr (p < .05). In conclusion, the SpermVital^® technology is improved and is more efficient in conserving post‐thaw sperm quality and results in higher sperm viability over time in vitro for SV than for C and B semen.  相似文献   

Bordetella bronchiseptica: a re-assessment of its role in canine respiratory disease   总被引：3，自引：0，他引：3  

N G Wright  H Thompson  D Taylor  H J Cornwell 《The Veterinary record》1973,93(18):486-487

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Continuous low level feed administration of pyrantel tartrate in lambs. II. Comparison with routine drenching in the prophylaxis of natural infections     

R L Cornwell  R M Jones 《The British veterinary journal》1969,125(7):330-339

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Activity of pyrantel against Parascaris equorum     

R L Cornwell  R M Jones 《The Veterinary record》1969,85(7):196-197

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Canine distemper: current concepts in laboratory and clinical diagnosis   总被引：6，自引：0，他引：6  

N G Wright  H J Cornwell  H Thompson  I M Lauder 《The Veterinary record》1974,94(5):86-92

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Immunity to dictyocaulus viviparus in calves. Vaccination with larvae attenuated by triethylene melamine (TEM) or x-irradiation and protection against field challenge     

R L Cornwell  R M Jones 《Research in veterinary science》1970,11(6):569-574

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木制家具出口呼唤“中国品牌”     

享尔 《国际木业》2007,37(3):28-29

近两年，我国木制家具出口可以说是超常规发展。2004年我国出口木制家具为129亿件，到了2005年，达到了1．49亿件，增幅高达16％；至2006年，尽管国际上欧美一些发达国家对我国家具出口“反倾销”和知识产权侵权诉讼接连不断，国内下半年又有国家出口退税率下调等显然不利于家具出口的政策出台，但所有这些内外在因素的合力，似乎都未能构成对我国家具出口大潮本质上的阻扼。  相似文献   

Experimental Infection of Rainbow Trout,Oncorhynchus mykiss,and Hybrid Striped Bass,Morone chrysops ♂ × Morone saxatilis ♀, with Viral Hemorrhagic Septicemia Virus Genotype IVb     

Emily R. Cornwell  Eileen A. Penner  Rodman G. Getchell  Geoffrey H. Groocock  Paul R. Bowser 《Journal of the World Aquaculture Society》2013,44(5):669-681

The emergence of a new sublineage of viral hemorrhagic septicemia virus (VHSV) within the Laurentian Great Lakes has caused concern for aquaculture in the United States. Because of the occurrence of VHSV in a new geographic location, new host species have been identified and the complete host range has not been determined. This study confirmed the high resistance of rainbow trout, Oncorhynchus mykiss, to VHSV type IVb infection. In addition, the experimental susceptibility of hybrid striped bass, Morone chrysops ♂ × Morone saxatilis ♀, to VHSV type IVb infection was examined but determined to be highly dependent on age of fish and exposure temperature. No mortality was observed in adult fish infected via intraperitoneal (IP) injection at 15 C, whereas yearling fish infected via IP injection under the same conditions experienced 20.8% mortality. Among yearling fish infected via IP injection, mortality increased to 100% when exposure to VHSV occurred at 10 C. An LD₅₀ for yearling hybrid striped bass exposed to VHSV at 10 C by IP injection was determined to be 1.4 × 10⁴ pfu (SE = 2.1). Thus, at 10 C, yearling hybrid striped bass experience a high mortality when exposed to VHSV IVb by IP injection.  相似文献   

Studies of parvovirus vaccination in the dog: the performance of live attenuated feline parvovirus vaccines     

H Thompson  I A McCandlish  H J Cornwell  L Macartney  N S Maxwell  A F Weipers  I R Wills  J A Black  A C Mackenzie 《The Veterinary record》1988,122(16):378-385

The performance of three live attenuated feline parvovirus vaccines licensed for use in the dog was studied. At the end of the primary vaccination course 67 per cent of dogs had inadequate antibody levels (less than or equal to 32) as measured by a haemagglutination inhibition test. Interference by maternal antibody accounted for some of the failures but the fact that there was no significant difference in performance between dogs vaccinated at 12 weeks or 16 weeks of age indicated that maternal antibody was not the only factor.  相似文献   

Presence of antibody cross-reacting with canine parvovirus in the sera of dogs from Greece   总被引：2，自引：0，他引：2  

G Koptopoulos  O Papadopoulos  M Papanastasopoulou  H J Cornwell 《The Veterinary record》1986,118(12):332-333

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