Recombinant Strain A/HK/Otar/6:2/2010 (H3N8) for Development of a Live Intranasal Equine Influenza Vaccine     

Olga V. Chervyakova  PhD  Vitaliy M. Strochkov  Kulyaisan T. Sultankulova  Nurlan T. Sandybayev  Abylay R. Sansyzbay  Nikolay E. Gorev  Maria V. Sergeeva  Marina V. PotapchukLudmila M. Tsybalova  DMSc  Oleg I. Kiselev 《Journal of Equine Veterinary Science》2014

Recombinant cold-adapted strain A/HK/Otar/6:2/2010 (H3N8) constructed by the method of classic genetic reassortment, inherited the hemagglutinin and neuraminidase genes from the epizootic A/equine/Otar/764/2007 (Н3N8) virus and genes of nonglycosylated proteins from attenuation donor A/Hong Kong/1/68/162/35 (H3N2). The resulted virus was of cold-adapted (efficient growth at 25°C) and temperature-sensitive (restricted replication at 39°C) phenotype. Like the parental virus of subtype H3N2, the resulted reassortant was attenuated, with limited replication in lungs of mice (1.75 lgEID₅₀/mL) versus replication in turbinate (2.5 lgEID₅₀/mL). Intranasal immunization of mice with A/HK/Otar/6:2/2010 (H3N8) reassortant did not induce animal weight reduction in contrast to epizootic A/equine/Otar/764/2007 (Н3N8) virus.  相似文献