Validation of a human immunoturbidimetric assay to measure canine albumin in urine and cerebrospinal fluid.     

Fabio Gentilini  Francesco Dondi  Cinzia Mastrorilli  Massimo Giunti  Claudia Calzolari  Gualtiero Gandini  Danilo Mancini  Paolo Famigli Bergamini 《Journal of veterinary diagnostic investigation》2005,17(2):179-183

The aim of this study was to validate an automated immunoturbidimetric assay used to quantify human albumin in urine and to accurately measure canine albumin concentrations in both urine and cerebrospinal fluid. The partial homology existing between human and canine albumin limited the accuracy of the human assays in measuring canine albumin without method modifications. Thus, the assay was modified by calibrating the analyzer with calibrators made in the laboratory containing known concentrations of canine albumin. To prepare the set of calibrators, the albumin concentration of pooled sera of healthy dogs was assessed in 5 replicates using the BromocresolGreen assay. Pooled samples were aliquoted and serially diluted to obtain the expected concentrations of albumin (0.5, 1, 5, 13, and 30 mg/dl) for establishing the canine calibration curve. Thereafter, the performance was assessed by analyzing canine urine and CSF The modified assay accurately quantified canine albumin in both specimens, as indicated by the following. Intra- and interassay variability was 0.92% and 2.74%, respectively; recovery was 99.66% and 99.07% in urine and 105.02% in CSF No interference was detected when hemolysate and glucose were added to urine. The test was linear within the verified range (0-225 mg/dl). These results demonstrate that the modified human albumin immunoturbidimetric assay can be a useful tool in the veterinary diagnostic laboratory. It is accurate and tends itself to automatization on chemistry analyzers.  相似文献   

Productive and biochemical responses of durum wheat to UV filtration     

Daniele Grifoni  Marco Napoli  Francesco Sabatini  Simone Orlandini  Marco Mancini  Gaetano Zipoli  Anna Dalla Marta 《Journal of Agronomy and Crop Science》2019,205(4):422-432

A UV exclusion experiment was conducted on durum wheat (Triticum durum Desf. var. Claudio) grown in pots. Plants were grown under three different radiative treatments in greenhouses covered with plastic filters: Teflon, transparent to the entire region of natural UV‐visible sunlight (TEF); polyester, transparent above 312 nm (MYL, excluding UVB) and Lee, transparent above 400 nm (LEE, excluding both UVA and UVB). Analyses have been carried out to determine the concentration of photosynthetic pigments in leaves, UV‐absorbing compounds, nitrogen and carbon in leaves, culms and spikes and proteins and gluten in grains. In particular, plants grown under UV exclusion showed a reduction in protein and dry gluten content (consequently without variation in the ratio dry gluten/protein), but at the same time, a significant increase of gluten index, which is a parameter to define the quality of gluten, was observed. The results highlighted the influence played by UV radiation on some biochemical parameters, mainly UV‐absorbing compounds, leaf nitrogen and grain protein characteristics of durum wheat cultivated under Mediterranean conditions. In particular, natural level of UV in the Mediterranean improves the characteristics of durum wheat flour important for pasta production (high dry gluten level), while the UV exclusion could improve characteristics of flour important for bread production (high gluten index).  相似文献   

Production,chemical composition,and fatty acid profile of milk from buffaloes fed with cupuaçu (Theobroma grandiflorum) cake and murumuru (Astrocaryum murumuru) cake in the Eastern Amazon     

André Guimarães Maciel e Silva  Suely Cristina Gomes de Lima  Pedro Danilo de Oliveira  Michel dos Santos Moraes  Célia Maria Costa Guimarães  Jamile Andréa Rodrigues da Silva  Alexandre Rossetto Garcia  Benjamim de Souza Nahúm  Lilaine de Sousa Neres  Gerlane Nunes Noronha  José de Brito Lourenço Júnior 《Animal Science Journal》2021,92(1):e13576

The objective was to evaluate the effect of concentrate supplementation using by-products of the Amazonian industry on milk production, milk composition, and milk fatty acid profile of dairy buffaloes. Twelve lactating buffaloes (544.5 ± 35.6 kg, 6.4 ± 2.2 years old, 59 ± 6 days in milk) were allotted in a pasture of Mombaça grass and managed under rotational grazing (4 days occupancy/28 days rest). A 3 × 3 Latin square was adopted, and each animal alternately received three supplementary treatments based on corn bran + soybean meal or cupuaçu cake or murumuru cake for 21 days per treatment. Murumuru cake increased the levels of lauric acid and myristic acid in the milk (p < 0.05). Murumuru cake reduced the unsaturated fatty acid contents in the milk compared with animals fed control diet or cupuaçu cake (24.27% vs. 25.24% vs. 25.08%). The n-6/n-3 ratio was 2.6, 1.97, and 2.0 in the control, cupuaçu, and murumuru groups, respectively. Based on this parameter, cakes made from cupuaçu as well as murumuru could be considered to be adequate for inclusion in dairy water buffalo feed. However, the murumuru cake addition requires some caution because its use induces the secretion of higher levels of lauric and myristic fatty acids that are related to human cardiovascular disease.  相似文献   

Effects of accumulate dead and alive insects in trap on the capture ofTribolium castaneum (herbst)     

P. Trematerra  F. Fontana  M. Mancini 《Anzeiger für Sch?dlingskunde》1996,69(1):3-9

In the trapping ofTribolium castaneum (Herbst), the activity of traps baited or not baited with aggregation pheromone 4,8-dimethyldecanal is influenced by the presence in the traps of accumulate specimens trapped. This fact has been observed both in trap containing alive or dead specimens. Interactions in the different substances produced by adults of Red Flour Beetle during their communication relationship are involved in this behaviour.The results obtained in the trials suggests that the presence of alive insects in traps is attractive for other free specimens, but under conditions of overcrowding quinone produced by them regulate population density by counteracting the effect of the aggregation pheromone, causing the insects to disperse or suppressing aggregation behaviour.  相似文献   

Prevalence of antibodies against Ornithobacterium rhinotracheale in broilers and breeders in Southern Brazil     

Canal CW  Leão JA  Ferreira DJ  Macagnan M  Pippi Salle CT  Back A 《Avian diseases》2003,47(3):731-737

In this investigation, we determined the prevalence of the Ornithobacerium rhinotracheale (ORT) infection in broilers and broiler breeders in southern Brazil. We also correlated the presence of antibodies in broilers with performance. Sera from 1550 broilers from 50 flocks were collected during the slaughter time in nine companies with federal veterinary inspection of the state of Rio Grande do Sul. Sera from 480 meat-type breeders of 40 flocks from 14 companies in southern Brazil were also analyzed by enzyme-linked immunosorbent assay, and the prevalence of antibodies was determined. The prevalence of ORT antibodies in broiler flocks was 63.83%, but in each individual flock only 6.52% of the birds were positive. The prevalence in broiler breeder flocks was 100.00%, and in each individual flock 94.62% of the birds were positive. There was a positive correlation between the presence of antibodies to ORT and decreased body weight in broilers. There was no significant correlation between presence of antibodies to ORT and age, lineage, efficiency index, feed conversion, and mortality. There was a positive correlation between the presence of respiratory signs and antibodies to ORT, although the reverse correlation was not significant. These results confirm that ORT is present and widespread in broilers and broiler breeders in southern Brazil.  相似文献   

Effects of accumulate dead and alive insects in trap on the capture ofTribolium castaneum (herbst)     

Prof Dr. P. Trematerra  Dr. F. Fontana  M. Mancini 《Journal of pest science》1996,69(1):3-9

In the trapping ofTribolium castaneum (Herbst), the activity of traps baited or not baited with aggregation pheromone 4,8-dimethyldecanal is influenced by the presence in the traps of accumulate specimens trapped. This fact has been observed both in trap containing alive or dead specimens. Interactions in the different substances produced by adults of Red Flour Beetle during their communication relationship are involved in this behaviour.The results obtained in the trials suggests that the presence of alive insects in traps is attractive for other free specimens, but under conditions of overcrowding quinone produced by them regulate population density by counteracting the effect of the aggregation pheromone, causing the insects to disperse or suppressing aggregation behaviour.The presence of dead insects in the traps, baited or not baited with pheromone, can be repellent for other free specimens ofT. castaneum, probably related to the presence of a residual alarm pheromone produced by the specimens before of death.With 10 figures and 3 tables  相似文献   

Epidemiological Analysis of Leptospira spp. Infection in Equids from the Brejo Paraibano Microregion of Brazil     

Ruy B. de Oliveira Filho  Karla C. Malta  Júnior M.B. Oliveira  Vania L. Assis Santana  Mabel H.V. Harrop  Danilo T. Stipp  José W. Pinheiro Júnior 《Journal of Equine Veterinary Science》2014

Leptospirosis is a zoonotic disease that occurs in humans and several animal species, including cattle, pigs, and horses, and it has a worldwide distribution. In equids, leptospirosis manifests through recurrent uveitis and abortion and other reproductive disorders. The objective of the present study was to investigate the epidemiological situation of Leptospira spp. infection in equids from the Brejo Paraibano microregion in the northeast of Brazil, that is, to assess the prevalence of and identify the major risk factors for infection. For this purpose, blood samples were taken from 257 equids (204 horses, 46 mules, and 7 donkeys), and a microscopic agglutination test (MAT) was used as serological analysis, with 24 serovars of Leptospira spp. as antigens. Antibodies were observed in 16.2% of horses, 13.0% of mules, and 28.6% of donkeys. Animals between the ages of 2.5 and 11 years had a risk of infection 0.4 times lower than those younger than 2.5 years old (odds ratio, 0.4; 95% confidence interval, 0.18-0.90). Further studies are needed to identify potential wild reservoirs and the main routes of infections.  相似文献   

A modelling approach to infer the effects of wind farms on landscape connectivity for bats     

Federica Roscioni  Hugo Rebelo  Danilo Russo  Maria Laura Carranza  Mirko Di Febbraro  Anna Loy 《Landscape Ecology》2014,29(5):891-903

Little is known about the potentially disrupting effects of wind farms on the habitat connectivity of flying vertebrates at the landscape scale. We developed a regional-scale model to assess the wind farm impact on bat migration and commuting routes. The model was implemented for the bat Nyctalus leisleri in a region of central Italy currently undergoing considerable wind farm development. A Species Distribution Model (SDM) for N. leisleri was generated using the MaxEnt algorithm based on 47 presence records (reduced to 19 after the autocorrelation procedure) and 10 environmental variables derived from topographic and land cover maps. We used the SDM to create a map of connectivity using the software UNICOR to identify potential commuting corridors (PCCs). The incidence of each wind farm on bat flight corridors was assessed by overlaying the existing (380) and planned (195) turbine locations onto the PCCs. The SDM was statistically robust (AUC > 0.8). Most of the corridors were concentrated in the western part of the region, which hosts the largest suitable areas for the species; most of the existing (54 %) and planned (72 %) wind farms interfered with important corridors connecting the western and the eastern parts of the region. Our results provide key information on the impact of the wind farm industry on biodiversity on a regional scale. The novel approach adopted, based on SDM and connectivity analysis, could be easily extended to other flying vertebrates and landscapes and constitutes a promising planning tool necessary for harmonizing the development of renewable energy infrastructures with issues of biodiversity conservation.  相似文献   

Mitochondrial reduction of metmyoglobin: dependence on the electron transport chain     

Tang J  Faustman C  Mancini RA  Seyfert M  Hunt MC 《Journal of agricultural and food chemistry》2005,53(13):5449-5455

Reduction of ferric myoglobin (metmyoglobin, MetMb) to its ferrous form is important for maintaining fresh meat color because only reduced myoglobin can bind oxygen to form the consumer-preferred cherry red color in fresh meat. The objective of this study was to characterize an apparent mitochondria electron transport chain (ETC)-linked pathway for MetMb reduction in vitro. MetMb was reduced in the presence of mitochondria and succinate (p < 0.05); mitochondria or succinate alone did not facilitate MetMb reduction relative to controls (p > 0.05). Flushing samples with oxygen greatly decreased MetMb reduction, while flushing with argon increased MetMb reduction when compared with controls (p < 0.05). ETC inhibitors were used to localize the site where electrons became available for MetMb reduction. MetMb reduction was increased by rotenone addition and decreased by malonic acid (p < 0.05); the reduction was completely abolished by additions of antimycin A or myxothiazol when compared with controls (p < 0.05). These results suggest that electrons become available for MetMb reduction at a site(s) between complex III and IV. Mitochondrial ETC-linked MetMb reduction increased with increased mitochondrial density and succinate concentration (p < 0.05); the greatest MetMb reduction was observed at pH 7.2 and 37 degrees C, and ETC-linked MetMb reducing activity decreased with time postmortem (p < 0.05). These results indicate that ETC-linked MetMb reduction exists but would be minimally active in postmortem muscles.  相似文献   

Does water use restrict the location of industrial air polluters?     

Danilo Santini 《Water, air, and soil pollution》1975,5(2):185-194

The paper examines water use in industrial production as a cause of geographical clustering of major air polluting industries. It shows that the majority of particulate and sulfur oxide emissions in the State of Illinois is emitted by industries using more than five billion liters of water per establishment per year. It also shows that the majority of industrial air pollutants is emitted within urban areas which are on water bodies with high and consistent volumes of water available. These and other results cited in the paper imply that any policy directly or indirectly promoting geographic dispersal as a means of reducing air pollution concentrations and population exposure rates is likely to be limited in effectiveness. It is suggested that such a policy can be effective only if at least one of two specified conditions is met. However, these conditions do not, by themselves, insure the success of such a policy. Finally, it is observed that the conjunction of air pollution with industrial water use indicates that much closer attention is needed to the relationship between air pollution and water pollution policies.  相似文献