An antisense coat protein gene confers immunity to potato leafroll virus in a genetically engineered potato   总被引：2，自引：0，他引：2  

Andrzej Pałucha  Włodzimierz Zagórski  Mirosława Chrzanowska  Danuta Hulanicka 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(3):287-293

The Bzura commercial potato cultivar was transformed by sense or antisense constructs which included the coat protein gene of potato leafroll virus RNA. In the sense construct, the coat protein gene was preceded by a leader sequence shorter than that in the subgenomic RNA formed in infected cells. The antisense construct consisted of a sequence complementary to the first 2020 nucleotides of the subgenomic RNA. Selected transformants expressing viral RNA were resistant to virus challenge by viruliferous aphids. In one line, expression of the antisense RNA prevented virus infection even after grafting with scions from infected plants and therefore this transformant might be regarded as virus immune.  相似文献   

Identification of S-haplotypes of European cultivars of sour cherry     

Anna Lisek  Danuta Kucharska  Agnieszka Głowacka  Elżbieta Rozpara 《The Journal of Horticultural Science and Biotechnology》2017,92(5):484-492

The sour cherry is known to exhibit the phenomenon of gametophytic self-incompatibility which prevents self-fertilization. In sour cherry, besides self-incompatible cultivars, there often occur self-compatible cultivars. This is due to the occurrence of natural mutations of the S-RNase or SFB genes and, consequently, loss of functionality of S-alleles. Here we present the results of the identification of S-haplotypes of 21 cultivars of sour cherry from various regions of Europe. The analyses were performed using methods based on the amplification of intron I and intron II of the S-RNase gene and fragments specific to the individual alleles of the S-RNase or SFB genes. The tested cultivars were found to contain 15 S-haplotypes: S₁, S_1?, S₄, S₆, S_6m, S_6m2, S₉, S₁₂, S₁₃, S_13?, S₂₆, S₃₅, S_36a, S_36b, and S_36b2. The most frequently occurring S-haplotypes were S_13? (61.9%), S_36a (57.1%), and S₂₆ (47.6%). On the basis of the results, 17 of the 21 cultivars were deduced to be self-compatible. The results will be of use in the production of sour cherry fruit by facilitating the selection of suitable pollinating cultivars. The results are also expected to be useful in the breeding of new cultivars of sour cherry when selecting genotypes for crosses.  相似文献   

The analysis of PAHs nitrogen derivatives in the sewage sludges of upper Silesia, Poland     

Danuta Bodzek  Beata Janoszka  Lidia Warzecra 《Water, air, and soil pollution》1996,89(3-4):417-427

The dimethylformamide extracts from sewage sludges from a chosen sewage — treatment plant in highly industrialized region of Poland were investigated. Using the column chromatography with Al₂O₃ and silicic acid as the stationary phases, the fraction of azaarenes has been separated. GC-MS analysis of azaarenes fraction made it possible to identify 10 azaarenes and several of their alkyl derivatives, which dominate in the organic matter extracted from sewage sludges. The silicic acid column chromatography in connection with the reduction of nitroarenes and derivatization with pentafluoropropionic acid anhydride yielded an enriched nitrofraction. Nitroarenes present in this fraction as amide derivatives were also analysed by GGMS method. There was found presence of 3 nitroarenes and their methyl derivatives.  相似文献   

Use of Enrichment,and Contamination Factors Together with Geoaccumulation Indexes to Evaluate the Content of Cd,Cu, and Ni in the Rybnik Water Reservoir in Poland   总被引：1，自引：0，他引：1  

Loska  Krzysztof  Cebula  Jan  Pelczar  Jacek  Wiechuła  Danuta  Kwapuliński  Jerzy 《Water, air, and soil pollution》1997,93(1-4):347-365

In this paper, the contamination degree of the Rybnik Reservoir with cadmium, copper and nickel was analyzed. Quality of the water from the reservoir was determined by drawing comparisons between the metal content in the water and both the officially permitted levels (contamination factor) and levels of metals occurring in the water of non-contaminated areas (enrichment factor). Contamination of bottom sediment with chosen metals was analyzed with reference to the metal content in mudstone (geoaccumulation index, enrichment factor, contamination factor). Trends towards changing the metal content in the bottom sediment was analyzed by determining the enrichment factor of the surface layer of the bottom sediments in relation to a deeper layer. Enrichment of the bottom sediments with metals coming from the water was also determined.  相似文献   

Molecular mapping of the AOX1a and β‐AmyI genes in potato     

Dominika Krusiewicz  Henryka Jakuczun  Iwona Wasilewicz‐Flis  Danuta Strzelczyk‐Żyta  Waldemar Marczewski 《Plant Breeding》2011,130(4):500-502

  相似文献   

Speciation of Cadmium in the Bottom Sediment of Rybnik Reservoir     

Loska  Krzysztof  Wiechuła  Danuta 《Water, air, and soil pollution》2002,141(1-4):73-89

Forms of cadmium occurrence in the bottom sediment of a heated water reservoir, polluted substantially with this metal was analysed in the paper. Apart from the analysis mentioned above, a total cadmium content in the bottom sediment and a content of cadmium dissolved in the water of the reservoir was also determined. Dependences between particular forms of cadmium occurrence and its total content in the bottom sediment were analysed. An influence of cadmium content in water on the level of particular cadmium forms in the bottom sediment and an influence of other parameters of the reservoir's water on the content of cadmium in the bottom sediment were also examined.  相似文献   

Veränderungen der Muskelfasertypenverteilung im M. longissimus dorsi von Ebern während des Wachstums, dargestellt mit verschiedenen histochemischen Methoden     

J. Wegner    Ilse  Fiedler  Danuta  Klosowska  B. Klosowski  Brigitte  Ziegan 《Anatomia, histologia, embryologia》1993,22(4):355-359

Changes in the distribution of muscle-fiber-types from the M. longissimus dorsi of male pigs. A Histochemical Approach Samples of the M. longissimus dorsi from growing up pigs were taken by a shooting-biopsy. The types of muscle-fibers could be demonstrated by two different histochemical staining procedures. With these two histochemical staining procedures significant differences between the different structure of the muscle-fibers during increasing age of the pigs could be detected.  相似文献   

The epidemiology of human enterohemorrhagic Escherichia coli infections in Lower Saxony     

Dreesman J  Pulz M 《DTW. Deutsche tier?rztliche Wochenschrift》2004,111(8):317-320

BACKGROUND: In the German federal state Lower Saxony, data on infections with enterohemorrhagic Escherichia coli (EHEC) and hemolytic uremic syndrome (HUS) are collected systematically since 1997 based on reports by physicians and laboratories. Initially the data were collected by means of a specific established surveillance system, since 2001 they are collected as part of the new infectious disease law. RESULTS: From 1997 to 2003, in Lower Saxony 880 EHEC-infections and 112 HUS-cases, 6 of whom died, were notified. This corresponds to an incidence of 1.6 EHEC-infections and 0.2 HUS-cases per 100000 person-years. No secular trend was observ-ed for the yearly number of HUS-cases, but raised numbers were observed for the years 1997 and 2002. There were strong regional differences of the EHEC/HUS-incidence from 0.7 in the district Braunschweig to 3.5 in the district Weser-Ems. For 56% of reported EHEC-cases, serotype information was available. The most frequent serogroup was O157, accounting for 30% of cases with serotype information. Of HUS-cases with known serotype, 86% were caused by O157, 20% by O157:H-. 52% of the EHEC-cases were less than 5 years old (HUS: 77%), and 68% less than 10 years (HUS: 92%). 23% of EHEC/HUS-cases could be identified as part of clusters with a mean number of 2.6 cases per cluster. These clusters almost exclusively affect-ed families. Though the data also contained information on possible sources or routes of infection, for none of the cases a microbiologically assured source was documented. CONCLUSIONS: The incidences of EHEC and HUS were higher in Lower-Saxony than in the whole of Germany. During the study period no significant trends concerning the number of HUS-cases or the distribution of serotypes were observed. More frequent serotyping and more complete information on sources or risks of infection should be achieved.  相似文献   

Occurence of PAHs in Various Elements of Environment in Zabrze (Upper Silesia,Poland)     

Bodzek  Danuta  Luks-Betlej  Krystyna  Janoszka  Beata 《Water, air, and soil pollution》1998,103(1-4):91-100

The Polycyclic Aromatic Hydrocarbons (PAHs) pollution state of the chosen town (Zabrze) of Upper Silesia, the most industrialised region in Poland, was presented. Zabrze is a town of medium size, having population twenty times bigger than a medium town polulation in Poland. In this study we determined the qualitative and quantitative composition of PAHs fraction separated from the following elements of environment: suspended dust, drinking water, soil and sewage sludge. The following analytical techniques were used: Soxhlet Extraction, Solid Phase Extraction, Gas Chromatography-Mass Spectrometry, Capillary Gas Chromatography and High Performance Liquid Chromatography. The results confirmed the significant state of examined region pollution. They stated the exceed of contents B(a)P in suspended dust. The soils in that place were heavily polluted with PAHs, about 4000 µg · kg-¹ for the sum of PAHs. The total contents of PAHs in sewage sludge of Zabrze did not exceed the analogous contents published for West European sewage sludge treatment plants and the level of determined PAHs in drinking water did not show any claims.  相似文献   

Comparison of Polycyclic Aromatic Compounds and Heavy Metals Contents in Sewage Sludges from Industrialized and Non-Industrialized Region     

Bodzek  Danuta  Janoszka  Beata 《Water, air, and soil pollution》1999,111(1-4):359-369

The total contents of PAHs, organic polar fractions and 14 major hydrocarbons containing 3–6 aromatic rings as well as heavy metal contents were determined in sewage sludges from highly industrialized (Upper Silesia), agricultural and recreational regions of Poland. Sludges from the industrial region showed markedly increased concentrations of all the organic micropollutants and 2–10 times higher levels of the following heavy metals: Pb, Cd, Cr, Cu, Fe, Zn, Ni, Mn and Co. The levels, however, did not exceed the Polish as well as European Economic Community limit values, which renders all the sludges applicable to land and some of them suitable for utilization on agricultural soils.  相似文献