Enzyme Immunoassays for the Determination of Ovine LH and FSH   总被引：2，自引：0，他引：2  

GM Peclaris  A Pappa  K Deligiannis  K Koutsotolis 《Reproduction in domestic animals》2003,38(5):367-372

The development of competitive enzyme immunoassays for ovine plasma LH (oLH) and FSH (oFSH) is described. Standards and plasma samples were preincubated with diluted antiserum to oLH or oFSH and the reacted solution (100 μl per well) was transferred to plates previously coated with oLH or oFSH, respectively. The second antibody used was anti‐rabbit IgG horseradish peroxidase. The measuring range was 0.39–50 ng/ml for each hormone and the 50% relative binding sensitivity was 9 ng/ml for oLH. The respective value for oFSH was 3.5 or 34 ng/ml with different hormone and antibody preparations used for the assay. The enzyme immunoassays were used to determine oLH and oFSH levels in plasma from ewes of two breeds during the oestrous cycle. The assays detected the first FSH surge coincident with the LH surge, the second FSH surge about 24 h later and the periodic fluctuations of FSH concentrations during the luteal phase of the oestrous cycle. These enzyme immunoassays are an efficient and economic alternative to the established radioimmunoassays (RIA) for oLH and oFSH.  相似文献   

Littoral spawning habitats of three southern Arctic charr (Salvelinus alpinus L.) populations     

Jonathan J. Low  Fran Igoe  John Davenport  Simon S. C. Harrison 《Ecology of Freshwater Fish》2011,20(4):537-547

Abstract Arctic charr populations in southern latitudes are nonmigratory, with all life‐stages limited to freshwater lakes and in‐ or out‐flowing tributaries. Although many of these populations are reported to also spawn in lake littorals, little is known about the physical characteristics of putative spawning grounds. A total of 23 discrete spawning sites within three Irish lakes were located by fyke netting of spawning adults and snorkelling in littoral habitats. Spawning sites were found to be long, narrow strips running parallel to the shore at a maximum depth of 124 cm. Spawning sites were limited to areas of coarse mineral substrate with an adequate (c. 8 cm) depth of clean interstitial spaces. In individual lakes, combined areas of spawning sites made up 0.4–0.7% of available littoral. Egg densities varied considerably between sites (33–900·eggs m^?2) and were significantly correlated with gradient and width of spawning sites. No evidence of redd digging was found. The shallow, localised and restricted nature of spawning grounds makes such populations vulnerable to anthropogenically induced postoviposition changes in surface water level, eutrophication processes such as increased lake sedimentation and elevated nutrient status.  相似文献   

Biophysical Society meeting. Crossover research yields scents and sensitivity     

Davenport RJ 《Science (New York, N.Y.)》2001,291(5511):2071-2072

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Reversibility of HIV drug resistance     

Davenport MP 《Science (New York, N.Y.)》2000,288(5470):1299

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Haemorrhagic enteritis of turkeys     

KE ARZEY  GM CROSS 《Australian veterinary journal》1990,67(11):413-413

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Clinicopathologic findings resembling hypoadrenocorticism in dogs with primary gastrointestinal disease   总被引：1，自引：0，他引：1  

S P DiBartola  S E Johnson  D J Davenport  J C Prueter  D J Chew  R G Sherding 《Journal of the American Veterinary Medical Association》1985,187(1):60-63

Nine dogs with primary gastrointestinal disease had clinical and laboratory findings resembling hypoadrenocorticism. The dogs had histories of anorexia, weakness or lethargy, diarrhea, vomiting, and weight loss. Hypothermia, dehydration, and emaciation also were detected on physical examination. Hyponatremia, hyperkalemia, and abnormally low Na/K ratios were found on laboratory evaluation, but results of ACTH-response tests were not compatible with hypoadrenocorticism. The primary diagnoses were trichuriasis and salmonellosis in 2 dogs, trichuriasis in 5 dogs, and perforated duodenal ulcer in 2 dogs. Most dogs responded to medical or surgical treatment of their primary gastrointestinal disease, and the original electrolyte abnormalities resolved. These findings emphasize the importance of the ACTH-response test in the diagnostic evaluation of dogs with clinicopathologic findings similar to those of hypoadrenocorticism.  相似文献   

COOPERATION IN SCIENCE     

Davenport CB 《Science (New York, N.Y.)》1907,25(636):361-366

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THE RESEARCHES OF ALFRED GOLDSBOROUGH MAYOR     

Davenport CB 《Science (New York, N.Y.)》1922,56(1440):134-135

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A REPLY TO DR. HERON'S STRICTURES     

Davenport CB 《Science (New York, N.Y.)》1913,38(987):773-774

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INVESTIGATIONS AT COLD SPRING HARBOR     

Davenport CB 《Science (New York, N.Y.)》1900,12(297):371-373

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