Isolation of Leptospira noguchii from sheep     

Silva EF  Brod CS  Cerqueira GM  Bourscheidt D  Seyffert N  Queiroz A  Santos CS  Ko AI  Dellagostin OA 《Veterinary microbiology》2007,121(1-2):144-149

The main goal of this study was to obtain new isolates of Leptospira spp. from sheep. A total of 10 kidney samples and 44 blood samples were collected from sheep slaughtered in Pelotas, Southern Brazil. One isolate was obtained which was identified by 16S rRNA gene sequencing and serogrouping to be Leptospira noguchii serogroup Autumnalis. Microscopic agglutination test (MAT) evaluation revealed that 4.5% of the sheep sera reacted against the Autumnalis serogroup. This is the first report of isolation of L. noguchii from sheep. Together these findings indicate that L. noguchii infections may be a potentially important veterinary problem in this domestic animal species.  相似文献   

Molecular diversity of Moraxella bovis isolated from Brazil, Argentina and Uruguay over a period of three decades     

Conceição FR  Dellagostin OA  Paolichi F  Leturia AC  Gil Turnes C 《Veterinary journal (London, England : 1997)》2004,167(1):53-58

The molecular profile of 30 Moraxella bovis strains, recovered from outbreaks of infectious bovine keratoconjunctivitis in Argentina, Brazil and Uruguay between 1974 and 2001, was determined through randomly applied polymorphic DNA (RAPD) analysis. Molecular profiles of nine strains recovered after 1990 varied from those recovered before 1990. The profiles of 13 strains (48%) differed from those of three vaccinal strains extensively used since 1984 in Argentina and Uruguay. Eight Argentinean strains, one from Brazil and two from Uruguay had identical RAPD profiles. Strains belonging to different serogroups had identical RAPD profiles, demonstrating that this technique was not able to discriminate among strains with low cross-reactivity indices. RAPD may be helpful in the primary characterization of M. bovis strains, but it does not replace serological characterization.  相似文献   

Molecular and serological characterization of Leptospira interrogans serovar Canicola isolated from dogs, swine, and bovine in Brazil     

Fabiana Miraglia  Zenaide M. de Morais  Odir A. Dellagostin  Fabiana K. Seixas  Julio C. Freitas  Francielle G. S. Zacarias  Ádina C. Delbem  Thaís S. P. Ferreira  Gisele O. Souza  Rudy A. Hartskeerl  Silvio A. Vasconcellos  Andrea M. Moreno 《Tropical animal health and production》2012,45(1):117-121

The identification of Leptospira clinical isolates through genotyping and serotyping, besides the recognition of its reservoirs, are important tools for understanding the epidemiology of leptospirosis, and they are also keys for identifying new species and serovars. Fourteen clinical isolates from animals were characterized by means of single enzyme amplified length polymorphism, variable number of tandem repeat analysis, pulsed field gel electrophoresis, and serotyping. All isolates were identified as Leptospira interrogans, serovar Canicola. Infections by this serovar occur in urban regions, where dogs represent the main maintenance hosts, whereas bovine and swine may act as reservoirs of serovar Canicola in rural areas. Both urban and rural aspects of leptospirosis, and the role of domestic animals as maintenance hosts, cannot be neglected in developing and developed countries.  相似文献   

Increased growth hormone (GH), growth hormone receptor (GHR), and insulin-like growth factor I (IGF-I) gene transcription after hyperosmotic stress in the Brazilian flounder Paralichthys orbignyanus     

Karina M. Meier  Márcio A. Figueiredo  Michel T. Kamimura  Jomar Laurino  Rodrigo Maggioni  Luciano S. Pinto  Odir A. Dellagostin  Marcelo B. Tesser  Luís A. Sampaio  Luis F. Marins 《Fish physiology and biochemistry》2009,35(3):501-509

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Uterine Progesterone Receptor and Leukaemia Inhibitory Factor mRNA Expression in Canine Pregnancy     

S Schäfer-Somi  D Klein  HB Beceriklisoy  S Sabitzer  SS Ay  AR Agaoglu  I Kücükaslan  D Kaya  OA Aksoy  S Aslan 《Reproduction in domestic animals》2009,44(S2):109-114

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Multiply-primed rolling-circle amplification (MPRCA) of PCV2 genomes: Applications on detection, sequencing and virus isolation     

Diogenes Dezen  Franciscus Antonius Maria Rijsewijk  Thais Fumaco Teixeira  Carine Lidiane Holz  Samuel Paulo Cibulski  Ana Cláudia Franco  Odir Antonio Dellagostin  Paulo M. Roehe 《Research in veterinary science》2010,88(3):436-440

Multiply-primed rolling-circle amplification (MPRCA) was used to amplify porcine circovirus type 2 (PCV2) genomes isolated from tissues of pigs with signs of post-weaning multisystemic wasting syndrome (PMWS). Two of the amplified PCV2 genomes were cloned in prokaryotic plasmids and sequenced. Both were nearly identical (1767 nt) except for one silent substitution in the region coding for the capsid protein (ORF2). In addition, they showed high nucleotide sequence similarity with PCV2 isolates from others countries (93–99%). To investigate whether the MPRCA amplified PCV2 genomes could be used to produce infectious virus, the cloned genomes were isolated from the plasmids, recircularized and used for transfection in PK-15 cells. This procedure led to the production of infectious virus to titres up to 10^5.55 TCID₅₀/mL. It was concluded that MPRCA is a useful tool to amplify PCV2 genomes aiming at sequencing and virus isolation strategies, where particularly useful is the fact that it allows straightforward construction of PCV2 infectious clones from amplified genomes. However, it was less sensitive than PCR for diagnostic purposes.  相似文献   

Multiple strains of Mycobacterium bovis revealed by molecular typing in a herd of cattle     

Figueiredo EE  Ramos DF  Medeiros L  Silvestre FG  Lilenbaum W  Silva JT  Paschoalin VM  Dellagostin OA 《Veterinary journal (London, England : 1997)》2012,193(1):296-298

Mycobacterium bovis isolates from an outbreak of bovine tuberculosis in a herd of cattle in Rio de Janeiro, Brazil, were analysed by spoligotyping and variable-number tandem repeat PCR analysis of the mycobacterial interspersed repetitive unit and exact tandem repeats. Molecular typing revealed a high genetic diversity of strains in the herd. The genetic diversity could be explained by the introduction of infected animals from different sources.  相似文献   

Detection of porcine Circovirus type 2 (PCV2) variants PCV2-1 and PCV2-2 in Brazilian pig population     

Janice Reis Ciacci-Zanella  Luciano S. Pinto  Lana Teixeira Fernandes  Odir Antonio Dellagostin 《Research in veterinary science》2009,87(1):157-232

In the present study whole genome of six Brazilian isolates of PCV2 were sequenced, analyzed and compared with 35 other sequences (24 from other countries and 17 from Brazil). The phylogenetic analysis showed that mostly Brazilian variants of PCV2 were grouped as PCV2-1. Two isolates among the six analyzed here could not be grouped with any other PCV2-2 analyzed in this study. One of these isolates was from an aborted fetus with myocarditis and the other from a PMWS affected pig. The results pointed here showed that both groups of PCV2 are present in Brazilian pig population without any clear geographical correlation.  相似文献   

Monoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions     

Monte LG  Conceição FR  Coutinho ML  Seixas FK  da Silva EF  Vasconcellos FA  deCastro LA  Hartleben CP  Dellagostin OA  Aleixo JA 《Comparative immunology, microbiology and infectious diseases》2011,34(5):441-446

Leptospirosis is an infectious disease caused by pathogenic spirochetes of the genus Leptospira that affects humans and a wide variety of animals. Recently the genomes of Leptospira interrogans, Leptospira borgpetersenii and Leptospira biflexa species were sequenced allowing the identification of new virulence factors involved in survival and pathogenesis of bacteria. LigA and LigB are surface-exposed bacterial adhesins whose expression is correlated with the virulence of Leptospira strains. In this study, we produced and characterized five monoclonal antibodies (MAbs) against a recombinant fragment of LigB (rLigBrep) with approximately 54 kDa that comprise the portions of LigA and LigB (domains 2–7). The 5 MAbs obtained were of the IgG1 (2) and IgG2b (3) isotypes and their affinity constants for rLigBrep ranged from 7 × 10⁷ M⁻¹ to 4 × 10⁸ M⁻¹. The MAbs were able to react with the native antigen on the L. interrogans, L. borgpetersenii and Leptospira noguchii surfaces by indirect immunofluorescence, immunoblotting and immunoelectron microscopy. These results demonstrate that the MAbs anti-rLigBrep can be useful to complement genetic studies and to aid studies aiming understanding the role of Lig proteins in Leptospira pathogenesis and the development of Lig-based vaccines and improved diagnostic tests for leptospirosis.  相似文献   

Vascular Endothelial (VEGF) and Epithelial Growth Factor (EGF) as Well as Platelet‐Activating Factor (PAF) and Receptors are Expressed in the Early Pregnant Canine Uterus     

S Schäfer‐Somi  S Sabitzer  D Klein  E Reinbacher  H Kanca  HB Beceriklisoy  OA Aksoy  I Kucukaslan  HC Macun  S Aslan 《Reproduction in domestic animals》2013,48(1):20-26

The aim of this study was to investigate the course of expression of platelet‐activating factor (PAF), PAF‐receptor (PAF‐R), epidermal growth factor (EGF), EGF‐R, vascular endothelial growth factor (VEGF), VEGF‐R1 and VEGF‐R2 in uterine tissue during canine pregnancy. For this purpose, 20 bitches were ovariohysterectomized at days 10–12 (n = 10), 18–25 (n = 5) and 28–45 (n = 5) days after mating, respectively. The pre‐implantation group was proven pregnant by embryo flushing of the uterus after the operation, the others by sonography. Five embryo negative, that is, non‐pregnant, bitches in diestrus (day 10–12) served as controls. Tissue samples from the uterus (placentation sites and horn width, respectively) were excised and snap‐frozen in liquid nitrogen after embedding in Tissue Tec^®. Extraction of mRNA for RT‐PCR was performed with Tri‐Reagent. In the embryos, mRNA from all factors except VEGF was detected. In the course of pregnancy, significantly higher expression of PAF and PAFR as well as VEGF and VEGFR2 during the pre‐implantation stage than in all other stages and a strong upregulation of EGF during implantation were characteristic. The course of EGF was in diametrical opposition to the course of the receptor. These results point towards an increased demand for VEGF, EGF and PAF during the earliest stages of canine pregnancy.  相似文献