To clarify the morphological and immunohistochemical characteristics in mares with granulosa theca cell tumor (GTCT), the localization of inhibin subunits (alpha, betaA, betaB) and aromatase in the granulosa cell layers and theca layers in the ovarian follicles were determined by immunohistochemical staining. The follicles were obtained from the ovaries of 6 mares with GTCT and 4 normal mares as controls. Immunohistochemically, inhibin alpha-subunit was localized in the granulosa cells of all follicles showing different sizes in all GTCT cases and betaA- subunit was localized in two GTCT cases in all sized follicles. But inhibin betaB- subunit and aromatase were not localized in GTCT cases. On the other hand, inhibin alpha-, betaA-, and betaB-subunits and aromatase were localized in the large and medium sized follicles, but inhibin betaA- and betaB-subunits and aromatase were not stained in the small sized follicles in normal cases. These findings suggest that some mares with GTCT can secrete dimeric inhibin (inhibin A), but all GTCT cases cannot secrete inhibin B. By the results of aromatase staining it is clear that testosterone is not converted into estradiol due to the lack of aromatase in the GTCT follicles. 相似文献
The present study was primarily carried out to trace the behavioral, physiological, and adrenal changes during the first stage of labor in cases of dystocia, either in parturient buffalo heifers or cows, and their clinical significance. Of the 118 investigated buffalo herds, 40 cows (dystocia = 20 and eutocia = 20) and 40 heifers (dystocia = 20 and eutocia = 20) were used in this study. Cows approaching actual birth were transferred to a calving box with straw bedding. Behavior of the studied animals was recorded from the time that the animal was moved to the calving box until the emergence of the fetal limbs in the vulval lips. If no progress in parturition was observed within two hours after the rupture of fetal sacs (end of the first stage), these cases were considered as suffering dystocia. As the fetal limbs emerged from the birth canal, animals were examined clinically to determine their average pulse and respiratory rates as well as body temperature. Blood samples were collected to determine the cow/heifers cortisol level. Results of the present study indicated that dystocia was accompanied by disturbed physiological status and cortisol levels. Practical monitoring system of parturient animals, through observing behavioral, physiological, and adrenal aspects during calving, should be regarded to expect dystocia and provide help for the animal in the appropriate time. 相似文献
Tropical Animal Health and Production - This article was originally published with incorrect affiliation 1. The correction affiliation is presented below. 相似文献
A survey of 7122 dromedary camels in 115 herds in Saudi Arabia was used to estimate the effects of herd size (HZ; <?25 vs. 25–49 vs. 50–100 vs. >?100 camels), herder/camels ratio (H/C; 1:<?25 vs. 1:25–50 vs. 1:>?50), manager experience (ME; <?5 vs. 5–10 vs. >?10 years), male/females ratio (M/F), housing system (HS; free vs. closed vs. mixed), length of the breeding season (winter vs. winter and spring vs. fall, winter and spring), age at first mating (3 vs. >?3 years), and time of mating after parturition (≤?3 vs. >?3 months) and their interactions on the overall pregnancy rate. Barren females of these herds (n?=?886) were examined for the causes of infertility. Results showed that herds with H/C of 1:<?25 had higher overall pregnancy rate (95.29%) than herds with H/C of 1:25–50 (79.84%) and those with H/C of 1:>?50 (72.79%) (p?=?0.003). Herds having ME of >?10 years revealed greater overall pregnancy rate (94.89%) than herds with ME of 5–10 years (80.54%) and those with ME of <?5 years (72.5%) (p?=?0.001). There were significant interactions between H/C × HZ (p?=?0.003), H/C × HS (p?=?0.006), and ME × HS (p?=?0.02). The overall pregnancy rate did not significantly differ between herds bred females by age of 3 years and those bred females by age >?3 years and in females bred within 3 months after parturition and in those bred after 3 months. The mean calving interval was shorter (p?=?0.008) in camels mated within 3 months of parturition (15.25?±?2.8 months) than in those mated after that time (24.33?±?6.5 months). Clinical endometritis, ovarian hydrobursitis, and vaginal adhesions were the common clinical findings in barren females. Thus, efforts to reduce the age at first mating and the interval after calving, increase the number of herders/camels, and control reproductive disorders could improve the reproductive performance and quality of camel herds in Saudi Arabia.
The functions of placental oestrogens during equine pregnancy are still unclear. Yet, they may act predominantly as local regulators of growth and differentiation in the microplacentomes. Thus, expression patterns of oestrogen receptors (ERs) α and β were investigated in the microcotyledonary placenta from pregnant mares at 110, 121, 179, 199 and 309 days of gestation by immunohistochemistry. In microplacentomes, both the ER isoforms were detected in trophoblast (T) cells, chorionic villous stroma (FS), microcaruncular epithelium (ME) and microcaruncular stroma (MS). Proportions of positive cells were 38–91% (T), 11–41% (FS), 55–89% (ME), 17–51% (MS) for ERα and 66–76% (T), 21–37% (FS), 41–68% (ME) and 24–55% (MS) for ERβ. Between days 110 and 199, proportions of cells positive for progesterone receptor (PR) varied between 19% and 62% (T), 3% and 50% (CS), 15% and 46% (ME), and 4% and 33% (MS). At day 309, PR was virtually absent in T, CS and ME (percentages < 0.1), whereas in MS 14.3% of cells were still positive. The expression of ERs and PR in equine microplacentomes gives evidence for a role of placental steroids as regulators of placental growth, differentiation and function. The detection of ERα, ERβ and PR in foetal and maternal vascular tissue suggests that placental steroids are also involved in the control of placental angiogenesis and /or vascular functions. The co-localization of ERs with aromatase in T suggests auto- or intracrine functions of oestrogens in this cell type. 相似文献
The objective of this study was to evaluate the use of transabdominal ultrasonography as a method for pregnancy diagnosis and establish some foetal biometrics guidelines of pregnancy staging. Transabdominal ultrasonography was performed weekly on six pregnant camels from the 4th to the 19th week and at 2‐week intervals from the 20th to the 52nd week of gestation. Six areas were selected for examination: caudal abdominal (CAA), inside the thigh, above the base of the udder; middle abdominal (MIA), from the base of the udder to the umbilicus; and cranial abdominal (CRA), from the umbilicus to the xiphoid cartilage. Several foetal parameters including orbital diameter (ORD), biparietal diameter (BPD), abdominal diameter (ABD), chest depth (CHD) and ruminal diameter (RUD) were measured using standardized scan plans. The relationships between the gestational age and the foetal parameters were evaluated. From the 6th to the 12th week, the conceptus was always observed through the left CAA approach. Between the 13th and 27th week, the foetus was entirely visualized by the MIA examination. From the 28th to the 52nd week, the conceptus was mainly detected through the left CRA approach. The foetal parameters were measured frequently during mid‐ and late gestation. All regression and correlation coefficients were highly significant (p < 0.0001). In conclusion, transabdominal ultrasonography was shown to be a reliable technique for pregnancy diagnosis and estimation of gestational age in dromedary camels. 相似文献
The objective of this study was to investigate the common forms of reproductive disorders and trends of fertility in cattle and buffaloes in Middle Egypt, and investigate nutritional deficiencies or imbalances as a cause of infertility. During the period from 2003 to 2006 a total of 4276 animals (2755 cattle and 1521 buffaloes) were examined, during winter and summer at three districts of Middle Egypt. Based on the owner complains, animals were categorized as anestrum, repeat breeder and those for pregnancy diagnosis. Feedstuffs of these districts were assessed for nutrient and mineral contents. Animals were examined by rectal palpation and by transrectal ultrasonography. The results showed that, ovarian inactivity was the most common cause of anestrum, whereas endometritis was the main frequent finding of repeat breeding. District, season and year affected the incidences of infertility. The pregnancy rate remained constant (cattle) or increased (buffaloes) from 2003 to 2006. The incidence of ovarian inactivity in both species decreased over the same period. In winter, the commonly used feedstuffs would be adequate to supply the animals with needs, but in summer, rations seem to be deficient in many essential nutrients. In conclusion, ovarian inactivity is the main cause of infertility in cattle and buffaloes in Middle Egypt. There is a link between poor nutrition in summer and the high incidence of ovarian inactivity. Fertility trends during the past few years are encouraging. 相似文献
Two pony mares were immunized against recombinant porcine inhibin alpha subunit three times with 39 day intervals. Clinical findings and endocrinological changes before immunization were taken as the control. The first significant rise in the anti-inhibin titre (P<0.05) in the circulation was found 27 days after the first injection. Maximum binding activity was reached by the 12th day after the second booster dose. The number of small, medium and large sized follicles had increased significantly compared to before immunization (11.75 +/- 4.30, 2.75 +/- 0.69 and 2.51 +/- 0.63 vs 6.50 +/- 1.43, 1.83 +/- 0.44 and 1.33 +/- 0.38, respectively), but the ovulation rate remained unchanged after immunization. The average plasma concentration of FSH and estradiol-17beta during the estrous cycle increased significantly (P<0.05) after immunization. These results suggest that immunization against inhibin is a useful tool to increase the number of ovarian follicles during the estrous cycle of pony mares. Moreover, the present study supported the concept that inhibin plays a major role in the control of follicular growth through its inhibitory effect on FSH secretion synergistically with steroid hormones. 相似文献
The aim of this study was to clarify the causes of scrotal enlargement in rams and bucks in Qassim region of Saudi Arabia. Enlarged scrotal contents of rams and bucks (n = 153) were examined by visual inspection, palpation, and ultrasonography. Blood samples were obtained and tested for Brucella sp. infection. Clinical and ultrasonographic findings showed that scrotal enlargement was mainly associated with orchitis, peri-orchitis, and epididymitis. Miscellaneous findings were scrotal hernia, scrotal hematoma, and hydrocele. The frequencies of orchitis, peri-orchitis, and epididymitis were 47.4, 21.1, and 14.1% in Awassi rams; 54.5, 21.7, and 8.7% in Najdi rams; 52.3, 20.5, and 9.1% in Ardi bucks; and 50, 16.7, and 16.7% in Damascus bucks, respectively. Orchitis was associated with no-abscess formation (23%), single-abscess formation (15.4%), and multiple-abscesses formation (61.6%). Peri-orchitis was characterized by hard consistency, atrophy of the testes, and extensive connective tissue formation. Epididymitis was observed mainly at the tail of the epididymis (82.4%) but rarely at the head (17.6%). Epididymitis was associated in many cases with abscessation (70.6%). Males with orchitis, peri-orchitis, and epididymitis were positive for Brucella melitensis and Brucella ovis in the frequency of 21.3% and 48.8%, respectively. In conclusion, scrotal enlargement in rams and bucks in Qassim region is caused mainly by inflammation of the testis and/or epididymis and associated tremendously with brucellosis seropositivity.
The nutritive value of soybean alone, supplemented with methionine+tryptophan or with bread was investigated. The results showed high Net Protein Utilization (N.P.U.) values for the autoclaved soybean diet (61.5) compared with that of raw soybean supplemented with methionine+tryptophan (57.7). It was found that rats fed the diets of raw soybean supplemented with methionine and tryptophan survived and the growth was improved to some extent.The N.P.U. values of the autoclaved soybean supplemented with methionine and tryptophan (67.4) was in close for that of casein (70.6).The effectiveness of soybean as a supplement to bread at a protein level of 50% was elucidated. The N.P.U. values of the autoclaved soybean supplemented with bread (65.8) was higher than that of either soybean or bread alone which showed the values of 61.5 and 47.0 respectively. 相似文献
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