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Metlholo Andries Phukuntsi Helene Brettschneider Desiré L Dalton Teresa Kearney Jacqueline Badenhorst Antoinette Kotze 《African Zoology》2016,51(1):69-76
DNA barcoding has been proposed as a method for species identification. However, this method has been criticised for its over-reliance on a single mitochondrial gene. In this study, four mitochondrial gene regions and one nuclear gene region were used to investigate their different abilities to identify tissue associated with museum specimens of Aethomys chrysophilus, Aethomys ineptus and Micaelamys namaquensis. Aethomys chrysophilus and the more recently elevated A. ineptus are indistinguishable on morphological grounds; however, their ranges are largely parapatric with only one syntopic locality currently known. All of the mitochondrial gene regions were able to separate M. namaquensis from A. chrysophilus and A. ineptus, but they varied in their abilities to resolve differences between A. chrysophilus and A. ineptus. The sequence results identified a specimen from KwaZulu-Natal that was misclassified and should have been identified as A. ineptus. Seven specimens that had not been reclassified following the elevation of A. ineptus to species level were identified as A. ineptus. Individuals of A. chrysophilus from Malawi could not be classified as either A. chrysophilus or A. ineptus, and may be a hybrid or a new, distinct species. This study indicates that DNA barcoding may be used to separate M. namaquensis from A. chrysophilus and A. ineptus, and although it was not able to separate A. chrysophilus and A. ineptus, it did indicate specimens from Malawi may be a new cryptic species. 相似文献
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Galvez J de Souza Gracioso J Camuesco D Galvez J Vilegas W Monteiro Souza Brito AR Zarzuelo A 《Fitoterapia》2006,77(7-8):515-520
Turnera ulmifolia is a plant popularly known in Brazil and South America as chanana. Some species of Turnera are widely used in folk medicine for different types of inflammatory diseases. In this study, the preventive intestinal antiinflammatory activity of a lyophilized infusion obtained from the aerial parts of T. ulmifolia was tested in the trinitrobenzenesulphonic acid (TNBS) model of rat colitis. The results obtained revealed that pretreatment to colitic rats with the extract, at 250 and 500 mg/kg, significantly attenuated the colonic damage induced by TNBS. This beneficial effect was associated with an improvement in the colonic oxidative status, since the infusion prevented the glutathione depletion that occurred as a consequence of the colonic inflammation. On the other hand, this antioxidant activity was confirmed in in vitro studies. In conclusion, the preventive effect exerted by the lyophilized infusion of T. ulmifolia in the TNBS model of rat colitis is probably related to its antioxidant properties, due to its flavonoids content. 相似文献
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Groeneveld Desirée Tekinerdogan Bedir Garousi Vahid Catal Cagatay 《Precision Agriculture》2021,22(4):1067-1106
Precision Agriculture - Farm management information system (FMIS) is an important element of precision agriculture to support the decision making process in the agricultural business. Developing... 相似文献
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Field experiments were conducted in clay and sandy clay loam soils using flat tillage blades of varying width, depth and rake angle. Measured quantities of draft force and disturbed soil areas for the different test conditions were compared to those predicted by a model of soil wedge failure in front of narrow blades, with reasonable agreement. Both the specific draft force per unit soil area and the degree of soil loosening were observed to increase with the relative narrowness of the tillage blades and with the rake angle, as predicted by the model. 相似文献
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- 1. The lagoon fishery of Rodrigues has considerable socio‐economic importance; however, catches have declined by 50% in recent years. The bluespine unicornfish, Naso unicornis (Forsskål 1775) is an important component of the fishery.
- 2. To begin to assess whether marine reserves might benefit this species, sonic telemetry was used to track individual fish in one of Rodrigues' four marine reserves. Seven unicornfish were caught in the Grand Bassin reserve and tagged with abdominally implanted acoustic tags.
- 3. Over a period of 57 days their locations were determined up to 21 times using a hand held hydrophone and receiver. Individual minimum convex polygon (MCP) home ranges varied from ～10 000 m2 to ～274 000 m2 and were not correlated with fish size. Kernel estimates of core areas (50% utilization distribution) varied from ～5000 m2 to ～175 000 m2. All seven fish stayed within the marine reserve and the largest home range occupied less than ～2% of the area of the marine reserve.
- 4. Fish remained on the outside edge of the lagoon on the shallow reef slope and among coral patches. These findings suggest that bluespined unicornfish biomass and numbers should increase if the Grand Bassin marine reserve is closed to fishing. Long‐term monitoring will, however, be required to demonstrate this outcome. Copyright © 2010 John Wiley & Sons, Ltd.
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Hernández L Afonso D Rodríguez EM Díaz C 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(4):408-415
Three hydroxybenzoic acids: p-hydroxybenzoic acid, vanillic acid and syringic acid; six hydroxycinnamic acids: p-coumaric acid, ferulic acid and four ferulic acid derivatives and a flavonoid (apigenin) were identified and quantified in
34 accessions corresponding to 19 cultivars of wheat applying HPLC coupled to diode array detector. Considerable differences
between the wheat cultivars were observed in the phenolic contents. Some cultivars (Colorado, Del País, Barbilla, Jallado,
Raspinegro Canario and Plaganudo) could be selected according to the high levels of phenolic compounds. Ferulic acid was the
major phenolic acid compound followed by syringic and p-hydroxybenzoic acids. The proportion of ferulic acid present as dimeric forms ranged from 4.2 to 8.6% across all of the wheat
cultivars analyzed. Apigenin, p-hydroxybenzoic and syringic acids did not show significant correlations. Many correlations between the determined hydroxycinnamic
acids were observed. The ferulic acid and all the ferulic acid derivatives showed highly significant correlations, suggesting
that the concentrations of diferulic acids depend on the concentration of ferulic acid. 相似文献
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Factors controlling the erosion rates in a semi-arid zone (Bardenas Reales,NE Spain) 总被引：1，自引：0，他引：1
Bardenas Reales is an erosive depression 415 km2 in area located in the central-western part of the Ebro Depression. Tertiary sediments crop out in the margin of the basin whereas Quaternary sediments, Holocene in age, occur only in its centre. These Holocene sediments (clays and silts) are the result of the erosion and weathering of the Tertiary clays and are made up of 3 different stratigraphic units. Erosion is clearly the dominant process in the centre of the depression, generating gullies up to 10 km in long and 8 m deep. In order to analyse and quantify the erosion rates of the studied area, erosion was measured on two Soil Erosion Plots with volumetric and instrumental techniques that have been supplied for the last 12 years. The results show that the estimated erosion rates depend extrinsically on the used method and intrinsically on the time of the year in which they are measured. The most accurate values are those obtained by means of collector devices. The values obtained by the microtopographic profile gauge are overestimated, while the results of the measurement with erosion pins are clearly lower. The study area is located in a semi-arid environment, with two pluviometric maximums, in spring and autumn, associated with convective storms. In summer and winter, rainfall is related to Atlantic cyclonal fronts. Soil loss is significant during the pluviometric maximums when rainfall quantity and intensity are higher. During cyclonal periods, in contrast, water erosion is null or scarce and soil loss is related to mudflows. In addition, the erosion rates of each Holocene unit are different. The C2 Holocene unit has undergone greater erosion because of its physico-chemical properties. All the stratigraphic units have similar characteristics regarding Tertiary materials. Since 1993 the average erosion rate measured by the collector device on the Tertiary materials is 32 Mg ha− 1 year− 1 and 77 Mg ha− 1 year− 1 in the Holocene. 相似文献
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Vanderwall DK Rasmussen DM Woods GL 《Journal of the American Veterinary Medical Association》2007,231(12):1864-1867
OBJECTIVE: To determine whether IM administration of exogenous oxytocin twice daily on days 7 to 14 after ovulation blocks luteolysis and causes prolonged function of corpora lutea (CL) in mares. DESIGN: Prospective study. ANIMALS: 12 mares. PROCEDURES: Beginning on the day of ovulation (day 0), jugular blood samples were collected every other day until day 40 for determination of progesterone concentration. On day 7, mares (n = 6/group) were treated with saline (0.9% NaCl) solution (control group) or oxytocin. Beginning on day 7, control mares received 3 mL of sterile saline solution every 12 hours, IM, and oxytocin-treated mares received 60 units of oxytocin every 12 hours, IM, through day 14. Mares were considered to have prolonged CL function if progesterone concentration remained > 1.0 ng/mL continuously through day 30. RESULTS: The proportion of mares with prolonged CL function was significantly higher in the oxytocin-treated group (6/6), compared with the control group (0/6). All control mares underwent luteolysis by day 16, at which time their progesterone concentrations were < 1.0 ng/mL. In contrast, all 6 oxytocin-treated mares maintained progesterone concentrations > 1.0 ng/mL continuously through day 30. CONCLUSIONS AND CLINICAL RELEVANCE: IM administration of 60 units of oxytocin twice daily on days 7 to 14 after ovulation was an efficacious method of inhibiting luteolysis and extending CL function in mares. Disrupting luteolysis by administering exogenous oxytocin during diestrus appears to be a plausible and practical method of long-term suppression of estrus in mares. 相似文献
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