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1.
Haematological and biochemical investigations were performed on 14 koalas with uncomplicated cystitis, 8 with complicated cystitis, 8 with conjunctivitis, 8 with lymphosarcoma, and 14 with miscellaneous diseases. Changes were limited and inconsistent in individual koalas with uncomplicated cystitis and conjunctivitis. In contrast, individual koalas with complicated cystitis were more likely to have anaemia, leukocytosis due to neutrophilia, hypoproteinaemia due to hypoalbuminaemia, and azotaemia due to elevated urea concentration. Although these changes were non-specific they did allow assessment of prognosis for survival and response to treatment. Koalas with lymphosarcoma were invariably anaemic, leukaemic, azotaemic and hypoalbuminaemic. Elevated enzymes (aspartate transaminase [AST]. lactate dehydrogenase [LD] and gamma glutamyl transferase [GGT]) were more common in koalas with lymphosarcoma. Koalas affected by miscellaneous conditions showed variable changes but once again anaemia, leukocytosis, azotaemia, elevated AST and LD, and hypoalbuminaemia were not uncommon. On the basis of these findings a minimal profile is suggested for the investigation of sick koalas and would include haematocrit, total and differential leukocyte counts, urea, total protein and albumin concentrations and AST, GGT and LD activities.  相似文献   
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OBJECTIVE: To evaluate effects of sedation on stability of resistance of the respiratory system (RRS) and measures of resting energy expenditure (REE) by use of open-flow indirect calorimetry (IC) and treatment with aerosolized albuterol on REE in horses with recurrent airway obstruction (RAO). ANIMALS: 9 clinically normal horses and 8 horses with RAO. PROCEDURE: In phase 1, RRS was measured by using forced oscillometry (FOT) in 5 clinically normal horses before and after sedation with xylazine. In phase 2, REE was measured in 4 clinically normal horses between 20 and 25 minutes and again 35 to 40 minutes after sedation with xylazine. In phase 3, IC was performed between 20 and 25 minutes and FOT was performed between 30 and 35 minutes after xylazine administration in 8 horses with RAO; after administration of 450 microg of albuterol, IC and FOT were repeated. RESULTS: In phase 1, RRS values were significantly lower 5 and 10 minutes after sedation. In phase 2, diminishing sedation did not significantly affect REE. In phase 3, there was a significant decrease in mean RRS (1.15 +/- 0.25 vs 0.84 +/- 0.14 cm H20/L/s) and REE (30.68 +/- 17.89 vs 27.46 = 16.54 kcal/kg/d) after albuterol administration. CONCLUSIONS AND CLINICAL RELEVANCE: FOT and IC are useful in obtaining repeatable measurements of RRS and REE, respectively, in sedated horses. Concurrent bronchodilation and decreased REE after albuterol administration suggest that increased work of breathing as a result of airway obstruction may contribute to increased energy demands in horses with RAO.  相似文献   
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Bonamia spp. parasites threaten flat oyster (Ostrea spp.) farming worldwide. Understanding test performance is important for designing surveillance and interpreting diagnostic results. Following a pilot survey which found low Bonamia sp. intensity in farmed Ostrea angasi, we tested further oysters (= 100–150) from each of three farms for Bonamia sp. using heart smear, histology and qPCR. We used a Bayesian Latent Class Model to assess diagnostic sensitivity (DSe) and specificity (DSp) of these tests individually or in combination, and to assess prevalence. Histology was the best individual test (DSe 0.76, DSp 0.93) compared to quantitative polymerase chain reaction (qPCR) (DSe 0.69, DSp 0.93) and heart smear (DSe 0.61, DSp 0.60). Histology combined with qPCR and defining a positive from either test as an infected case maximized test performance (DSe 0.91, DSp 0.88). Prevalence was higher at two farms in a high‐density oyster growing region than at a farm cultivating oysters at lower density. Parasite intensities were lower than in New Zealand and European studies, and this is probably contributed to differences in the performance of test when compared to other studies. Understanding diagnostic test performance in different populations can support the development of improved Bonamia surveillance programs.  相似文献   
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We investigated the efficacy of praziquantel (PZQ) and fenbendazole (FBZ), each administered by bath and orally, against the monogenean Lepidotrema bidyana Murray, a gill parasite of the freshwater fish silver perch, Bidyanus bidyanus (Mitchell). PZQ and FBZ were each administered by bath at 10 mg L?1 for 48 h and on surface‐coated feed pellets at 75 mg kg?1 per body weight (BW) per day for 6 days. Bath treatments of PZQ and FBZ had an efficacy of 99% and 91%, respectively, against adult L. bidyana. Oral treatments of PZQ and FBZ had an efficacy of 79% and 95%, respectively, against adult L. bidyana. Fish rejected feed pellets surface‐coated with PZQ, suggesting that palatability of surface‐coated PZQ‐medicated feed is poor, which undermined efficacy. In all trials, some juvenile parasites were present on fish after treatment during efficacy assessment, indicating that efficacy may be lower against juvenile parasites or that recruitment occurred post‐treatment, demonstrating that repeat treatments are necessary to effectively control L. bidyana in aquaculture.  相似文献   
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Dioncopseudobenedenia Yamaguti, 1965 (Monogenea: Capsalidae) is redefined. Dioncopseudobenedenia kala Yamaguti, 1965 (type species) is redescribed from type material from Hawaii and from new specimens from Heron Island, Queensland, Australia and New Caledonia. We made detailed observations on D. macracantha Yamaguti, 1968 from type material from Hawaii, and from new material from Heron Island and New Caledonia. Dioncopseudobenedenia ancoralis sp. n. is described from the gill chamber of Siganus lineatus (Valenciennes) from Green Island and Heron Island, Australia and from New Caledonia. This study confirms that only one pair of large central sclerites is present on the haptor in Dioncopseudobenedenia species. The male copulatory organ in species of Dioncopseudobenedenia is a penis contained in a fluid-filled space (= penis canal) with weakly muscular walls. Dioncopseudobenedenia kala and D. ancoralis bear a sclerite at the tip of the penis. In D. macracantha, the structure of the penis, which has no terminal sclerite, indicates it may combine the functions of a penis and a cirrus. Dioncopseudobenedenia is compared with Calicobenedenia Kritsky et Fennessy, 1999, the other capsalid genus with a single pair of large sclerites on the haptor. The large haptoral sclerites in species of Dioncopseudobenedenia resemble accessory sclerites, whereas those of C. polyprioni Kritsky et Fennessy, 1999 resemble hamuli. Observations of oncomiracidia confirmed that the large haptoral sclerites in D. kala are accessory sclerites. Haptoral morphology suggests that different Dioncopseudobenedenia spp. employ different means of attachment. Mating behaviour was observed twice between two different pairs of D. kala specimens from Heron Island. Two preserved specimens from Nouméa, New Caledonia had structures near the dorsal vaginal pore that we interpret as spermatophores. This is the first report of spermatophores in a capsalid inhabiting the gill chamber. The geographic distribution of Dioncopseudobenedenia spp. is discussed.  相似文献   
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Supplementation of exogenous growth hormone (GH) during prepuberty advances onset of spermatogenesis in boars, but the mechanism of action is unknown. The present study is an investigation of the presence and characteristics of testicular growth hormone receptors (GHR). A total of 36 boars were castrated, three boars every 10 days, between the ages of 10 and 120 days. Testicular membrane preparations of 10, 20, 30, 50, 70, 100 and 120‐day‐old boars were used to determine 125I‐bGH binding and Scatchard analysis. Liver from a 60‐kg barrow was used for comparison. Specific 125I‐bGH binding to testicular membrane preparations occurred in all age groups with the exception of 20‐day‐old boars at levels of 30–40% of liver binding. At 30 days of age the unlabelled bGH at 1.1 ng/tube achieved half maximal inhibition (ID50). Results of Scatchard analysis indicated a single class of binding sites. Binding affinity was 2.89 × 109m with a binding capacity of 12 fmole/mg membrane protein. The results from this study suggest that GH may act directly on the cells of the prepubertal boar testis.  相似文献   
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Three visible lesions were examined from two specimens of southern bluefin tuna. The lesions were examined grossly and two were identified as lipomas, the third bore similarities to a schwannoma. Histopathology confirmed that two consisted of mature adipocytes consistent with a diagnosis of lipoma. The third lesion consisted of spindle cells in Antoni A and B patterns and was tentatively diagnosed as a malignant schwannoma. Immunohistochemistry identified both S100 and glial fibrillary acid protein expression within the lesion, which, together with the histopathological appearance, is consistent with a diagnosis of neurofibrosarcoma.  相似文献   
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