QTL for IHNV resistance and growth identified in a rainbow (Oncorhynchus mykiss) × Yellowstone cutthroat (Oncorhynchus clarki bouvieri) trout cross     

Renata M. Barroso  Paul A. Wheeler  Scott E. LaPatra  Robert E. Drew  Gary H. Thorgaard   《Aquaculture (Amsterdam, Netherlands)》2008,277(3-4):156-163

Infectious hematopoietic necrosis virus (IHNV) is a major constraint to rainbow trout culture. Yellowstone cutthroat trout (Oncorhynchus clarki bouvieri) have greater resistance to this virus than do rainbow trout (O. mykiss), but the genetic mechanism of this resistance is not understood. We conducted a genome scan using a backcross of cutthroat trout into a rainbow trout background to estimate the number and locations of quantitative trait loci (QTL) associated with IHNV resistance and growth in trout. IHNV resistance was considered in terms of both survival (binary trait) and days to death (quantitative trait). The genetic map was scanned using interval mapping via two different approaches: one model considered survival alone and a second two-part model combined both survival and days to death. Three QTL were significantly (P ≤ 0.05) associated with virus resistance genome-wide, explaining 32.5% of the phenotypic variation. Cutthroat alleles at two of these QTL resulted in increased resistance to the pathogen, as expected. No growth QTL were detected in this cross. We suggest that these traits are genetically independent.  相似文献   

Serologic survey of domestic felids in the Petén region of Guatemala.     

Adrienne L A Lickey  Melissa Kennedy  Sharon Patton  Edward C Ramsay 《Journal of zoo and wildlife medicine》2005,36(1):121-123

Blood samples were analyzed from 30 domestic cats (Felis domesticus) from the Petén region of Guatemala to determine the seroprevalence of common pathogens that may pose a potential risk to native wild felids. Eight of the cats had been vaccinated previously; however, owners were unable to fully describe the type of vaccine and date of administration. In addition, blood samples were obtained from two captive margays (Leopardus wiedii). Samples were tested for antibodies to feline immunodeficiency virus, Dirofilaria immitis, feline panleukopenia virus, feline herpesvirus, feline coronavirus, canine distemper virus, and Toxoplasma gondii and for feline leukemia virus (FeLV) antigen. Fifty percent or more of the cats sampled were seropositive for feline herpesvirus (22 of 30), feline panleukopenia (15 of 30), and T. gondii (16 of 30). Five cats were positive for FeLV antigen. Both margays were seropositive for feline coronavirus and one was strongly seropositive to T. gondii. All animals were seronegative for D. immitis. This survey provides preliminary information about feline diseases endemic to the Petén region.  相似文献   

Renal adenocarcinoma, hepatocellular carcinoma, and pancreatic islet cell carcinoma in a binturong (Arctictis binturong).     

Eric Klaphake  Ahmed Shoieb  Ed Ramsay  Juergen Schumacher  Linden Craig 《Journal of zoo and wildlife medicine》2005,36(1):127-130

A 19-yr-old binturong (Arctictis binturong) with acute upper respiratory disease was euthanized. Postmortem findings included hepatocellular carcinoma, pancreatic islet cell carcinoma, and renal adenocarcinoma with metastasis to the spleen, pleura, and pericardium. A link between primary hepatic and renal neoplasms has been noted in older humans.  相似文献   

Dietary protein and the reproductive performance of cows     

Laven RA  Drew SB 《The Veterinary record》1999,145(24):687-695

Increasing a cow's intake of dietary protein intake can increase its milk production, but can also reduce its fertility. This paper reviews the effects of increasing dietary protein on the fertility of the dairy cow, and the mechanisms that may produce them. The effects vary widely, but all stages of the reproductive cycle from the return to cyclicity after parturition, to the survival of the embryo, may be affected. However, the underlying cause of the link between protein intake and fertility is unclear. Fertility could be reduced by a direct toxic effect of protein breakdown products, but alternatively the increased energy demand for their metabolism could be responsible. The effect of protein degradability is also uncertain. Excess rumen degradable protein is commonly associated with reduced fertility, but similar effects are produced by diets that contain excess rumen undegradable protein. Increasing the intake of protein of all degradabilities has significantly different effects on blood biochemistry than a reduction in the intake of energy, suggesting that not all the effects of protein are due to energy imbalance. The primary site of action of the effect is also unclear. Limited evidence suggests that it is localised to the reproductive system, but effects on the pituitary and hypothalamus, as well as the ovary and uterus, have all been postulated. It is also uncertain what toxic principle is involved. Ammonia, nitrate and urea have all been suggested, but there is no conclusive evidence. Although a high protein intake has been postulated to have an effect on fertility for over 30 years, the evidence remains inconclusive, and the aetiology and pathogenesis of the effect remain obscure.  相似文献   

THE EFFECT OF TIPPING OF HORNS AND INTERRUPTION OF JOURNEY ON BRUISING IN CATTLE     

W. R. Ramsay  B.V.Sc.  H. R. C. Meischke  B.V.Sc.  † B. Anderson  Q.D.A.H. 《Australian veterinary journal》1976,52(6):285-286

  相似文献   

Porcine leptin inhibits lipogenesis in porcine adipocytes   总被引：6，自引：0，他引：6  

Ramsay TG 《Journal of animal science》2003,81(12):3008-3017

The present study examined whether recombinant porcine leptin alters lipid synthesis in porcine adipocytes. The stromal-vascular cell fraction of neonatal pig subcutaneous adipose tissue was isolated by collagenase digestion, filtration, and subsequent centrifugation. These cells were seeded on 25-cm2 tissue culture flasks and proliferated to confluency in 10% (vol/vol) fetal bovine serum in Dulbecco's modified Eagle medium/F12 (DMEM/F12, 50:50). Cultures were differentiated using 2.5% pig serum (vol/vol), 10 nM insulin, 100 nM hydrocortisone. After 7 d of lipid filling, cultures were washed free of this medium, incubated overnight in DMEM/F12 containing 2% pig serum (vol/vol), and then used for experiments. Acute experiments assessed U-(14)C-glucose or 1-(14)C-palmitate metabolism in cultures exposed to porcine leptin (0 to 1,000 ng/mL medium) for 4 h. Chronic experiments used cultures incubated with 0 to 1,000 ng porcine leptin/mL medium for 44 h before measurements of U-(14)C-glucose and 1-(14)C-palmitate oxidation and incorporation into lipid. Another experiment examined whether chronic leptin treatment alters insulin responsiveness by including insulin (10 nM) with incubations containing leptin. Leptin had no acute effects on glucose oxidation or conversion to lipid (P > 0.05). Acute leptin treatment decreased palmitate incorporation into lipids up to 45% (P < 0.05). Chronic leptin exposure decreased glucose oxidation (21%), total lipid synthesis (18%), and fatty acid synthesis (23%) at 100 ng/mL medium (P < 0.05). Insulin increased rates of glucose oxidation, total lipid, and fatty acid synthesis (P < 0.05); however, chronic exposure to 10 ng leptin/mL medium decreased the effectiveness of 10 nM insulin to affect these measures of glucose metabolism by approximately 18 to 46% (P < 0.05). Higher concentrations of leptin inhibited all effects of insulin on glucose metabolism (P < 0.05). Chronic exposure to leptin increased palmitate oxidation by 36% (P < 0.05). Chronic leptin exposure decreased palmitate incorporation into total lipids by 40% at 100 ng/mL medium (P < 0.05). Lipoprotein lipase activity was not affected (P > 0.05) by leptin. These data indicate that leptin functions to promote partitioning of energy away from lipid accretion within porcine adipose tissue by inhibiting glucose oxidation and lipogenesis indirectly, by decreasing insulin-mediated stimulation of lipogenesis, and by stimulating fatty acid oxidation while inhibiting fatty acid esterification.  相似文献   

CSF virus in East Anglia: where from?     

Sandvik T  Drew T  Paton D 《The Veterinary record》2000,147(9):251

  相似文献   

GEOPHYSICS: Enhanced: A Strained Earth, Past and Present     

Ramsay JG 《Science (New York, N.Y.)》2000,288(5474):2139-2141

Plate tectonics leads to deformation within converging or transforming plates, but the rates at which this happens are difficult to evaluate. In this Perspective, Ramsay highlights a new technique reported by Müller et al. for overcoming this problem in past and present mountain-building regions, which have particularly complex deformation patterns.  相似文献   

Oral eosinophilic granulomas in tigers (Panthera tigris)--a collection of 16 cases.     

John M Sykes  Michael M Garner  Leah L Greer  Nancy P Lung  Rob L Coke  Frank Ridgley  Mitch Bush  Richard J Montali  Ben Okimoto  Robert Schmidt  Jack L Allen  Bruce A Rideout  Patricia A Pesavento  Edward C Ramsay 《Journal of zoo and wildlife medicine》2007,38(2):300-308

Oral eosinophilic granulomas were diagnosed in 16 tigers (Panthera tigris). All lesions were located on the hard or soft palate and typically consisted of flat or slightly raised circular ulcers. Histologic features of these lesions were essentially identical to those seen in oral eosinophilic granulomas of domestic cats and dogs. No clinical signs were noted in eight cases, though various degrees of inappetence, excessive salivation, and dysphagia were noted in the other eight tigers. Six cases were not treated. Treatment for the remaining 10 cases centered on corticosteroids and additional treatments included surgical removal, cryotherapy, antibiotics, and chlorpheniramine. Treatment with corticosteroids did appear to be effective in some cases, though lesions would worsen after cessation of therapy and no cases were cured. In addition, three cases developed complications possibly related to this corticosteroid therapy. The etiology of these lesions remains unknown, though an underlying allergic condition is likely.  相似文献   

AN UNDISCOVERED GAS     

Ramsay W 《Science (New York, N.Y.)》1897,6(144):493-502

  相似文献