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1.
Wagner M Hein I Grif K Sumerkan AB Esel D Allerberger F 《Berliner und Münchener tier?rztliche Wochenschrift》2003,116(11-12):460-466
Research on the pathogen Listeria monocytogenes is a key issue both for the clinical and the food microbiologist owing to the unique pathway of infection and the exposure of humans via contaminated foods. Although, in Austria, the incidence of listeriosis is about 870-fold lower than the incidence for Salmonella infection, the food law manages both foodborne pathogens with a comparable stringency. The current risk management is based on the assumption that environmental L. monocytogenes isolates, from which the pool of "foodborne" isolates is recruited, are of similar pathogenicity compared to clinical and outbreak isolates. This verdict became doubted in the recent years. Characterization of L. monocytogenes by virulence gene sequencing, virulence studies in vivo and in vitro and by molecular typing was considerably stimulating the discussion on virulence variability in L. monocytogenes. This article provides insights in the value of epidemiological follow-up studies by presenting a typing study on 15 cases of listeriosis observed in a district hospital in Turkey. Furthermore results from typing L. monocytogenes either by virulence gene sequencing, mismatch amplification mutation assay or by pulsed field gel electrophoresis are discussed. The close interaction of molecular microbiology with food microbiology both in applied and basic science is currently creating a new discipline of molecular food microbiology. We are convinced that veterinary medicine will contribute to this exiting development in a fruitful way. 相似文献
2.
A. C. Inkaya E. Yetim N. P. Acar S. Alp O. Erisoz Kasap F. Gunay E. M. Arsava B. Alten A. Ozkul D. Us M. Niedrig K. Ergunay 《Zoonoses and public health》2014,61(7):480-491
Toscana virus (TOSV), West Nile virus (WNV) and tickborne encephalitis virus (TBEV) are among major viral pathogens causing febrile disease and meningitis/encephalitis. The impact of these viruses was investigated at a referral centre in Ankara Province, Central Anatolia in 2012, where previous reports suggested virus circulation but with scarce information on clinical cases and vector activity. Serum and/or cerebrospinal fluid samples from 94 individuals were evaluated, in addition to field‐collected arthropod specimens that included 767 sandflies and 239 mosquitoes. Viral nucleic acids in clinical samples and arthropods were sought via specific and generic nested/real‐time PCRs, and antibody responses in clinical samples were investigated via commercial indirect immunofluorescence tests (IIFTs) and virus neutralization. A WNV antigen assay was also employed for mosquitoes. WNV neuroinvasive disease has been identified in a 63‐year‐old male via RNA detection, and the WNV strain was characterized as lineage 1. TOSV infections were diagnosed in six individuals (6.3%) via RNA or IgM detection. Partial sequences in a 23‐year‐old female, presented with fever and transient pancytopenia, were characterized as TOSV genotype A. Febrile disease with arthralgia and/or peripheral cranial nerve involvement was noted in cases with TOSV infections. Previous WNV and TOSV exposures have been observed in 5.3% and 2.1% of the subjects, respectively. No confirmed TBEV exposure could be identified. Morphological identification of the field‐collected mosquitoes revealed Culex pipiens sensu lato (74.4%), Anopheles maculipennis (20.9%), An. claviger (2.1%) and others. Sandfly species were determined as Phlebotomus papatasi (36.2%), P. halepensis (27.3%), P. major s. l. (19.3%), P. sergenti (8.9%), P. perfiliewi (4.4%), P. simici (2.6%) and others. Viral infections in arthropods could not be demonstrated. TOSV genotype A and WNV lineage 1 activity have been demonstrated as well as serologically proven exposure in patients. Presence of sandfly and mosquito species capable of virus transmission has also been revealed. 相似文献
3.
Ceren Kütahyal? ?enol Sert Berkan ?etinkaya Ezgi Yal??nta? M. Bahad?r Acar 《Wood Science and Technology》2012,46(4):721-736
The biosorption of Ce(III) from aqueous solution by citric acid-modified Pinus brutia leaf powder was studied in a batch system as a function of initial pH, temperature, initial concentration of adsorbate, and contact time. Central composite design method was used in the experiments. Thermodynamic parameters such as standard enthalpy (ΔH 0), entropy (ΔS 0), and free energy (ΔG 0) were calculated, and the results indicated that biosorption was exothermic. The biosorption of Ce(III) on modified Pinus brutia leaf powder was investigated by Freundlich, Langmuir, and Dubinin-Radushkevich (D-R) isotherms. The results show that Ce(III) adsorption can be explained by Langmuir isotherm model, and monolayer capacity was found as 62.1?mg/g. The results suggested that the modification process enhances the biosorption capacity of the adsorbent, and modified Pinus brutia leaf powder may find promising applications for the recovery of Ce(III) from aqueous effluents. 相似文献
4.
The efforts to mitigate CO2, one of the greenhouse gases causing global warming and climate change problems, have an important place in the worldwide agenda. One of the factors that cause CO2 emissions is energy use in residential buildings, hence urban residential use is one of the areas on which mitigation efforts are focused. As land use planning determines the spatial development, density and form of residential areas, it has a direct role in the formation of residential emissions. However, these efforts to mitigate emissions could not yet be integrated with land use planning. It is possible to list the reasons as the mitigation efforts are made either on the building scale (mitigation of residential emissions by technological–architectural solutions) or on a global–regional scale (development of carbon sinks), the focus on transportation sector, and in land use planning, lack of considering the potential of urban green areas to mitigate emissions. By taking into consideration the O2 production potential of green areas, a method proposal for land use planning is developed in this study to maintain the carbon–oxygen balance in residential areas. Depending on the form and density of the residential areas developed in a plan, the necessary size of green areas in such a plan is estimated by the method proposed. The varying emission amounts in different residential areas with diverse housing types, the number of actively used residential units, and local vegetation differences are included in the estimate. Therefore, an estimation method proposal that could be easily used in land use planning and that considers local differences is presented in the paper. 相似文献
5.
Macrolides are considered to be one of the safest anti-infective groups in clinical use, with severe adverse reactions being rare. However, there are limited data about their embryotoxicity and teratogenicity. We aimed to investigate and compare the effects of these agents on embryonic growth and development. Rat embryos were cultured in vitro for 48 h in rat serum. Whole rat serum was used as a culture medium for the control group while different concentrations of spiramycin and azithromycin (1.25-6.25 microg/ml), and clarithromycin (2.5-30 microg/ml) were added to rat serum for the experimental groups. Dose-dependent effects of macrolides on embryonic developmental parameters were compared using morphological methods. Embryos were evaluated for the presence of any malformations. After morphological examination of the embryos, total DNA was extracted from the cells using standard procedures to determine fragmentation of nuclear DNA of embryonic cells. When compared with the control embryos, the macrolides significantly decreased all growth and developmental parameters dose dependently. While clarithromycin was found to cause more developmental toxicity than spiramycin and azithromycin, azitromycin was determined to have more teratogenicity potential. Compared with controls, there was no difference regarding the fragmentation of nuclear DNA of all the agents used. According to these results, when the toxic and teratogenic potential of the used agents compared, because of the lower toxic and teratogenic effects observed with spiramycin, this agent may be preferred for parturients. 相似文献
6.
Orhan Corum Duygu Durna Corum Orkun Atik Feray Altan Ayse Er Kamil Uney 《Journal of veterinary pharmacology and therapeutics》2019,42(6):654-659
The pharmacokinetics and bioavailability of levamisole were determined in red‐eared slider turtles after single intravenous (IV), intramuscular (IM), and subcutaneous (SC) administration. Nine turtles received levamisole (10 mg/kg) by each route in a three‐way crossover design with a washout period of 30 days. Blood samples were collected at time 0 (pretreatment), and at 0.25, 0.5, 1, 1.5, 3, 6, 9, 12, 18, 24, 36, and 48 hr after drug administration. Plasma levamisole concentrations were determined by a high‐performance liquid chromatography assay. Data were analyzed by noncompartmental methods. The mean elimination half‐life was 5.00, 7.88, and 9.43 hr for IV, IM, and SC routes, respectively. The total clearance and volume of distribution at steady state for the IV route were 0.14 L hr?1 kg?1 and 0.81 L/kg, respectively. For the IM and SC routes, the peak plasma concentration was 9.63 and 10.51 μg/ml, respectively, with 0.5 hr of Tmax. The bioavailability was 93.03 and 115.25% for the IM and SC routes, respectively. The IM and SC route of levamisole, which showed the high bioavailability and long t1/2?z, can be recommended as an effective way for treating nematodes in turtles. 相似文献
7.
Ozgun Kalkisim Zuhal Okcu Besim Karabulut Duygu Ozdes Celal Duran 《Erwerbs-Obstbau》2018,60(2):173-181
The aim of the present research was to investigate the fruit quality of twenty different local pear varieties (Pyrus communis L.), namely Ahlat, Ankara, Arpa, B?ld?rc?n, Çermai, Cinci, Gelin Bo?an, Hac? Hamza, Hah?r, Kabak, K?z?l, Kokulu, Mehrani, Menendi, Sulu, ?algam, Tokat Sultan?, Tur?u, Yaz, and Yaz Meyri?i, grown in Gumushane province in terms of pomological and morphological characteristics and chemical compositions. The fruit mass, fruit width and length, fruit stem thickness and length, fruit kernel width and length, hardness of pulp, number of seeds, leaf width and length, leaf stem length and thickness, and water soluble dry matter (WSDM) of the pear fruits have been determined as pomological and morphological characteristics. On the other hand, the chemical compositions of the pear varieties have been evaluated in terms of protein, ash, sucrose, fructose, glucose, total sugar, titratable acidity, moisture, and mineral element levels.Both pomological and morphological results demonstrated that the local pear varieties are important in terms of rehabilitation studies and detailed selection studies on these local varieties should be performed. The chemical analyses result of the pear varieties revealed that there is no component that may be harmful to human health when consumed, and also these varieties contains the necessary amount of mineral elements. 相似文献
8.
Ozgun Kalkisim Duygu Ozdes Zuhal Okcu Besim Karabulut Hasan Basri Senturk 《Erwerbs-Obstbau》2016,58(1):41-48
The present study was conducted in order to determine the pomological and morphological characteristics and chemical compositions of some local apple varieties grown in Gumushane. As a result of field work in Gumushane province, sixteen different apples, which are Summer Apple, English apple, Green Belly, Black Belly and Yellow Belly Apples, Amasya Marble, Bey Apple, Chest Apple, Mahsusa, Arabian Girl, Willow Apple, Tav?anba??, Yellow H?d?r, Local Marble, Bride Apple, and Fatty Apple were collected in order to analyze. The average results of pomological and morphological properties and chemical compositions were obtained as following: fruit mass; 127.36 g, fruit width; 65.23 mm, fruit length; 57.30 mm, fruit stalk thickness; 2.17 mm, fruit stalk length; 17.63 mm, stalk pit depth; 12.75 mm, flower pit depth; 10.29 mm, fruit firmness; 13.79 kg/cm2, seed house width; 15.89 mm, seed house length; 17.39 mm, number of seeds; 4.3, water soluble dry matter content; 14.53?%, ash content; 1.11?%, pH; 3.8, titratable acidity; 0.69 g/L, water content; 83.8?%, reducing sugar; 5.04 g/100 g, sucrose; 2.25 g/100 g, and the amount of total sugars; 7.29 g/100. The fruit peel color was determined by considering the light transmittance (L). The average L values were ranged between 112.06 and 66.32. On the other hand among the mineral elements, potassium and manganese amounts were determined as the highest and the lowest, respectively. 相似文献
9.
This study was carried out in Arsin (Trabzon/Turkey) in 2011. The effects of different harvest time and altitudes on the quality of the nuts have been investigated. The study was performed on Fo?a hazelnut and the harvest process has been conducted at three terms, which are on normal harvest time and ten days before and after harvest time. The harvested nuts were dried in the shade on the concrete floor until their moisture content decreased to 5?%. Some properties of nuts including yield, fruit weight, internal weight, shell thickness, and protein, oleic, and linoleic acid amounts have been investigated. As evaluated all of the fruit properties it can be concluded that 11 August is the most suitable harvest date for coast zone. On the other hand, no significant differences were obtained in the point of protein, oleic, and linoleic acid amounts for different harvest time and altitudes. 相似文献
10.